TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.462
(823 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Aven... 207 2e-054
gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Aven... 206 3e-054
gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Aven... 205 6e-054
gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Aven... 205 8e-054
gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Aven... 205 8e-054
gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Aven... 203 3e-053
gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Aven... 157 2e-039
gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Aven... 116 6e-027
gb|CN816143.1|CN816143 HRO4517_E01_I01ZS5 Lib AA071E1X Aven... 96 4e-025
>gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4408_D06_H12, mRNA
sequence
Length = 415
Score = 207 bits (446), Expect = 2e-054
Identities = 91/93 (97%), Positives = 93/93 (100%)
Frame = +2 / +2
Query: 263 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 442
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSRE
Sbjct: 41 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 220
Query: 443 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSN*A 541
IQTSVRLVLPGELAKHAVSEGTKAVTKFTS+*A
Sbjct: 221 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*A 319
Score = 180 bits (388), Expect = 2e-046
Identities = 82/87 (94%), Positives = 83/87 (95%)
Frame = -2 / -2
Query: 528 VNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRASLAASPASFSKMSLMKEF 349
VNLVTALVPSETACLASSPGRTRRTEV ISR+VMVGFLL RASLA SPASFSKMSLMKEF
Sbjct: 306 VNLVTALVPSETACLASSPGRTRRTEVCISREVMVGFLL*RASLADSPASFSKMSLMKEF 127
Query: 348 MMDMALEDMPMSGCTCFSTLKM*IL*V 268
MMDMALEDMPMSGCTC STLKM*IL*V
Sbjct: 126 MMDMALEDMPMSGCTCLSTLKM*IL*V 46
>gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4527_G09_N17, mRNA sequence
Length = 412
Score = 206 bits (445), Expect = 3e-054
Identities = 91/92 (98%), Positives = 92/92 (100%)
Frame = +2 / +1
Query: 263 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 442
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE
Sbjct: 28 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 207
Query: 443 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSN* 538
IQTSVRLVLPGELAKHAVSEGTKAVTKFTS+*
Sbjct: 208 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 303
Score = 188 bits (406), Expect = 7e-049
Identities = 83/87 (95%), Positives = 86/87 (98%)
Frame = -2 / -3
Query: 528 VNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRASLAASPASFSKMSLMKEF 349
VNLVTALVPS+TACLASSPGRTRRTEVWISR+VMVGFLL RASLAASPASFSKMSLMKEF
Sbjct: 293 VNLVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLL*RASLAASPASFSKMSLMKEF 114
Query: 348 MMDMALEDMPMSGCTCFSTLKM*IL*V 268
MMDMALE+MPMSGCTCFSTLKM*IL*V
Sbjct: 113 MMDMALEEMPMSGCTCFSTLKM*IL*V 33
Score = 96.4 bits (204), Expect = 5e-021
Identities = 40/46 (86%), Positives = 41/46 (89%)
Frame = -1 / -2
Query: 415 VVPRQLGGLAGQLLKDVVDEGVHDGHGLGGYADVGVHLLQHLEDVD 278
VV RQLGGLA + LKDVVDEGVHDGHGLGG ADV VHLLQHLEDVD
Sbjct: 180 VVARQLGGLASEFLKDVVDEGVHDGHGLGGDADVRVHLLQHLEDVD 43
>gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_A08_B15, mRNA sequence
Length = 655
Score = 205 bits (443), Expect = 6e-054
Identities = 90/93 (96%), Positives = 93/93 (100%)
Frame = +2 / +3
Query: 263 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 442
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSRE
Sbjct: 81 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 260
Query: 443 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSN*A 541
IQTSVRLVLPGELAKHAVSEGTKAVTKFTS+*+
Sbjct: 261 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*S 359
Score = 179 bits (386), Expect = 4e-046
Identities = 77/85 (90%), Positives = 80/85 (94%)
Frame = -2 / -1
Query: 528 VNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRASLAASPASFSKMSLMKEF 349
VN VTALVPS+TACLASSPGRTRRTEVWISR+VMVGFLLY AS A SPASFSKMSLMKEF
Sbjct: 346 VNFVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLLYLASFADSPASFSKMSLMKEF 167
Query: 348 MMDMALEDMPMSGCTCFSTLKM*IL 274
MMDMALE+MPMSGCTCF TLKM*IL
Sbjct: 166 MMDMALEEMPMSGCTCFRTLKM*IL 92
>gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4507_C01_F01, mRNA sequence
Length = 365
Score = 205 bits (442), Expect = 8e-054
Identities = 90/92 (97%), Positives = 92/92 (100%)
Frame = +2 / +3
Query: 263 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 442
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSRE
Sbjct: 69 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 248
Query: 443 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSN* 538
IQTSVRLVLPGELAKHAVSEGTKAVTKFTS+*
Sbjct: 249 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 344
Score = 180 bits (388), Expect = 2e-046
Identities = 80/87 (91%), Positives = 82/87 (94%)
Frame = -2 / -2
Query: 528 VNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRASLAASPASFSKMSLMKEF 349
VN VTALVPSETACLASSPGRTRRTEVWISR+VMVGFLL RASLA SPASFSKMSLMKEF
Sbjct: 334 VNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFLL*RASLADSPASFSKMSLMKEF 155
Query: 348 MMDMALEDMPMSGCTCFSTLKM*IL*V 268
MMDMALE+MPMSGCTC TLKM*IL*V
Sbjct: 154 MMDMALEEMPMSGCTCLRTLKM*IL*V 74
>gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_B07_C14, mRNA
sequence
Length = 454
Score = 205 bits (442), Expect = 8e-054
Identities = 90/92 (97%), Positives = 92/92 (100%)
Frame = +2 / +1
Query: 263 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 442
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSRE
Sbjct: 13 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 192
Query: 443 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSN* 538
IQTSVRLVLPGELAKHAVSEGTKAVTKFTS+*
Sbjct: 193 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 288
Score = 180 bits (388), Expect = 2e-046
Identities = 80/87 (91%), Positives = 82/87 (94%)
Frame = -2 / -3
Query: 528 VNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRASLAASPASFSKMSLMKEF 349
VN VTALVPSETACLASSPGRTRRTEVWISR+VMVGFLL RASLA SPASFSKMSLMKEF
Sbjct: 278 VNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFLL*RASLADSPASFSKMSLMKEF 99
Query: 348 MMDMALEDMPMSGCTCFSTLKM*IL*V 268
MMDMALE+MPMSGCTC TLKM*IL*V
Sbjct: 98 MMDMALEEMPMSGCTCLRTLKM*IL*V 18
>gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4516_H02_P04, mRNA sequence
Length = 472
Score = 203 bits (438), Expect = 3e-053
Identities = 89/91 (97%), Positives = 91/91 (100%)
Frame = +2 / +2
Query: 266 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSREI 445
ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSREI
Sbjct: 11 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREI 190
Query: 446 QTSVRLVLPGELAKHAVSEGTKAVTKFTSN* 538
QTSVRLVLPGELAKHAVSEGTKAVTKFTS+*
Sbjct: 191 QTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 283
Score = 171 bits (368), Expect = 1e-043
Identities = 77/87 (88%), Positives = 80/87 (91%)
Frame = -2 / -2
Query: 528 VNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRASLAASPASFSKMSLMKEF 349
VNLVTALVPSETACLASSPG TRRTEVWIS +VMVGFLLY ASLA SPASFSK+SLMKEF
Sbjct: 273 VNLVTALVPSETACLASSPGSTRRTEVWISLEVMVGFLLYLASLADSPASFSKISLMKEF 94
Query: 348 MMDMALEDMPMSGCTCFSTLKM*IL*V 268
MMDMALE+MPMSG TC TLKM*IL*V
Sbjct: 93 MMDMALEEMPMSGWTCLRTLKM*IL*V 13
>gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_C10_F20, mRNA sequence
Length = 360
Score = 157 bits (338), Expect = 2e-039
Identities = 71/74 (95%), Positives = 73/74 (98%)
Frame = +2 / +2
Query: 320 GISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS 499
GISS AMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS
Sbjct: 11 GISS*AMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS 190
Query: 500 EGTKAVTKFTSN*A 541
EGTKAVTKFTS+*A
Sbjct: 191 EGTKAVTKFTSS*A 232
Score = 143 bits (307), Expect = 3e-035
Identities = 65/71 (91%), Positives = 67/71 (94%)
Frame = -2 / -1
Query: 528 VNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRASLAASPASFSKMSLMKEF 349
VNLVTALVPSETACLASSPGRTRRTEV ISR+VMVGFLL RASLA SPASFSKMSLMKEF
Sbjct: 219 VNLVTALVPSETACLASSPGRTRRTEVCISREVMVGFLL*RASLADSPASFSKMSLMKEF 40
Query: 348 MMDMALEDMPM 316
MMDMA EDMP+
Sbjct: 39 MMDMA*EDMPL 7
>gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_F01_K01, mRNA sequence
Length = 361
Score = 116 bits (247), Expect = 6e-027
Identities = 52/55 (94%), Positives = 54/55 (98%)
Frame = +2 / +3
Query: 374 EKLAGEAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSN* 538
EKLA E+AKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTS+*
Sbjct: 12 EKLACESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 176
Score = 104 bits (221), Expect = 2e-023
Identities = 46/52 (88%), Positives = 48/52 (92%)
Frame = -2 / -1
Query: 528 VNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRASLAASPASFS 373
VNLVTALVPSETACLASSPGRTRRTEVWIS +VMVGFLLY ASLA S A+FS
Sbjct: 166 VNLVTALVPSETACLASSPGRTRRTEVWISLEVMVGFLLYLASLADSQANFS 11
>gb|CN816143.1|CN816143 HRO4517_E01_I01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_E01_I01, mRNA sequence
Length = 384
Score = 95.9 bits (203), Expect(2) = 4e-025
Identities = 45/54 (83%), Positives = 47/54 (87%)
Frame = +2 / +2
Query: 416 KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSN*ARCGIRGSVRRVL 577
KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTS+* R GS R +L
Sbjct: 56 KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*VRFAPPGSGRWLL 217
Score = 33.6 bits (67), Expect(2) = 4e-025
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = +2 / +3
Query: 371 FEKLAGEAAKLARYNKKP 424
FEKL+GE+AKLAR+ + P
Sbjct: 12 FEKLSGESAKLARFTRSP 65
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,751,105
Number of Sequences: 8143
Number of extensions: 47555
Number of successful extensions: 410
Number of sequences better than 1.0e-020: 9
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 279987740
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)