TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.448
(911 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821219.1|CN821219 HRO4428_H01_P02ZS5 Lib AA069E1X Aven... 258 1e-069
gb|CN816185.1|CN816185 HRO4517_H07_O13ZS5 Lib AA071E1X Aven... 161 1e-040
gb|CN819635.1|CN819635 HRO4402_D11_G22ZS5 Lib AA069E1X Aven... 140 4e-034
>gb|CN821219.1|CN821219 HRO4428_H01_P02ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4428_H01_P02, mRNA
sequence
Length = 576
Score = 258 bits (557), Expect = 1e-069
Identities = 105/112 (93%), Positives = 111/112 (99%)
Frame = -1 / +3
Query: 692 FPARARRRFQRGLKRKPMALIKKLRKAKKDAPAGEKPEPVKTHLRNMIIVPEMIGSIVGV 513
FPARARRRF+RGLKRKPMAL+KKLRKAKKDAPAGEKPEPV+THLRNMIIVPEMIGS++GV
Sbjct: 12 FPARARRRFKRGLKRKPMALVKKLRKAKKDAPAGEKPEPVRTHLRNMIIVPEMIGSLIGV 191
Query: 512 YNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK*AV 357
YNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK* +
Sbjct: 192 YNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK*VM 347
Score = 234 bits (505), Expect = 2e-062
Identities = 100/109 (91%), Positives = 101/109 (92%)
Frame = +1 / -3
Query: 364 HFRGINREEWVAPIPGLPCLTGL*EMENSAR*WPIISGLISTWLKVLPL*TPTMLPIISG 543
H RG+NREEWVAPIPGLPC TGL*EMENSAR*WPIISGLISTWLKVLPL*TP LPIISG
Sbjct: 340 HLRGMNREEWVAPIPGLPCFTGL*EMENSAR*WPIISGLISTWLKVLPL*TPIRLPIISG 161
Query: 544 TMIMLRRCVLTGSGFSPAGASFFALRSFLMSAMGFLFRPLWNLLLARAG 690
TMIMLRRCVLTGSGFSPAGASFFALRSFL SAMGFL RPL NLL ARAG
Sbjct: 160 TMIMLRRCVLTGSGFSPAGASFFALRSFLTSAMGFLLRPLLNLLRARAG 14
Score = 198 bits (426), Expect = 1e-051
Identities = 83/112 (74%), Positives = 95/112 (84%)
Frame = +3 / -2
Query: 357 NSSFQRNKPRGVGGTDTGPAMLDRLIGDGELSKVVADHLRLDLNLVEGLAVVDPNNASNH 536
++SF+R++P GVGGTDTGP ML RL+GDGELSKV+A+HLRLDLNLVEGLA+VD + A+NH
Sbjct: 347 HNSFERDEPGGVGGTDTGPTMLHRLVGDGELSKVMANHLRLDLNLVEGLAIVDTDKAANH 168
Query: 537 LRDNDHVTEMCLDWLWFLTSRSILFRLAQLLDECHGLPLQTPLEPSPGARGE 692
LRDNDHV EMC L LTSR ILFRLAQLLDE HGLPL+T LEPS G GE
Sbjct: 167 LRDNDHVAEMCPHRLRLLTSRGILFRLAQLLDERHGLPLETSLEPSAGTGGE 12
Score = 188 bits (405), Expect = 1e-048
Identities = 80/111 (72%), Positives = 88/111 (79%)
Frame = -3 / +2
Query: 693 LPRARQEKVPEGSEEEAHGTHQEAAQGEKGCSCW*ETRASQDTSP*HDHCP*DDWKHCWG 514
+PR +KV E S+EEAHG QEAAQGEKGC CW*E A +DTSP HDHCP*DDW+
Sbjct: 11 VPRPCPQKVQERSQEEAHGARQEAAQGEKGCPCW*EA*ACEDTSPQHDHCP*DDWQPYRC 190
Query: 513 LQRQDLQPG*DQA*DDRPLPC*VLHLL*AGQAWQARYRCHPLLAVYSSEMS 361
LQ QDLQPG*DQA*DD PLPC*VLHLL A +AW ARYRCHPLL V+ S+MS
Sbjct: 191 LQWQDLQPG*DQA*DDWPLPC*VLHLLQACEAW*ARYRCHPLLPVHPSQMS 343
>gb|CN816185.1|CN816185 HRO4517_H07_O13ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_H07_O13, mRNA sequence
Length = 416
Score = 161 bits (347), Expect = 1e-040
Identities = 64/71 (90%), Positives = 69/71 (97%)
Frame = -1 / +2
Query: 575 VKTHLRNMIIVPEMIGSIVGVYNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGA 396
V+THLRNMII+PEMIGS+VG+YNGK FN VEIKPEMIGHYLAEFS+SYKPVKHGRPGIGA
Sbjct: 11 VRTHLRNMIIMPEMIGSVVGIYNGKAFNSVEIKPEMIGHYLAEFSLSYKPVKHGRPGIGA 190
Query: 395 THSSRFIPLK* 363
THSSRFIPLK*
Sbjct: 191 THSSRFIPLK* 223
Score = 137 bits (293), Expect = 3e-033
Identities = 60/72 (83%), Positives = 62/72 (86%)
Frame = +1 / -3
Query: 361 AHFRGINREEWVAPIPGLPCLTGL*EMENSAR*WPIISGLISTWLKVLPL*TPTMLPIIS 540
+H G+N EEWVAPIPGLPCLTGL*E ENSAR*WPIISGLIST LK LPL* PT LPIIS
Sbjct: 225 SHLSGMNLEEWVAPIPGLPCLTGL*ERENSAR*WPIISGLISTELKALPL*IPTTLPIIS 46
Query: 541 GTMIMLRRCVLT 576
G MIMLRR VLT
Sbjct: 45 GMMIMLRRWVLT 10
Score = 105 bits (224), Expect = 1e-023
Identities = 44/68 (64%), Positives = 58/68 (85%)
Frame = +3 / -2
Query: 363 SFQRNKPRGVGGTDTGPAMLDRLIGDGELSKVVADHLRLDLNLVEGLAVVDPNNASNHLR 542
S + N+P GVGGT+T P++LD L+G+GEL +VVA+HLRLDL+ VEGLAVVD ++ASNHLR
Sbjct: 223 SLEWNEP*GVGGTNTRPSVLDWLVGEGELCQVVANHLRLDLDGVEGLAVVDTDDASNHLR 44
Query: 543 DNDHVTEM 566
+DHV E+
Sbjct: 43 HDDHVAEV 20
>gb|CN819635.1|CN819635 HRO4402_D11_G22ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4402_D11_G22, mRNA
sequence
Length = 354
Score = 140 bits (300), Expect = 4e-034
Identities = 55/61 (90%), Positives = 59/61 (96%)
Frame = -1 / +3
Query: 545 VPEMIGSIVGVYNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK 366
+PEMIGS+VG+YNGK FN VEIKPEMIGHYLAEFS+SYKPVKHGRPGIGATHSSRFIPLK
Sbjct: 6 MPEMIGSVVGIYNGKAFNSVEIKPEMIGHYLAEFSLSYKPVKHGRPGIGATHSSRFIPLK 185
Query: 365 * 363
*
Sbjct: 186 * 188
Score = 119 bits (255), Expect = 6e-028
Identities = 51/61 (83%), Positives = 53/61 (86%)
Frame = +1 / -3
Query: 361 AHFRGINREEWVAPIPGLPCLTGL*EMENSAR*WPIISGLISTWLKVLPL*TPTMLPIIS 540
+H G+N EEWVAPIPGLPCLTGL*E ENSAR*WPIISGLIST LK LPL* PT LPIIS
Sbjct: 190 SHLSGMNLEEWVAPIPGLPCLTGL*ERENSAR*WPIISGLISTELKALPL*IPTTLPIIS 11
Query: 541 G 543
G
Sbjct: 10 G 8
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,182,150
Number of Sequences: 8143
Number of extensions: 122098
Number of successful extensions: 792
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 316507880
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)