TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.280
(3445 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815444.1|CN815444 HRO4509_G04_M07ZS5 Lib AA071E1X Aven... 234 6e-062
>gb|CN815444.1|CN815444 HRO4509_G04_M07ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_G04_M07, mRNA sequence
Length = 630
Score = 234 bits (506), Expect = 6e-062
Identities = 93/106 (87%), Positives = 102/106 (96%)
Frame = +2 / +3
Query: 2177 IIVDGVSGLHIDPYHSDKAADILVNFFDKCKADPSYWDKISQGGLQRIYEKYTWKLYSER 2356
IIV+ VSGLHIDPYHSDKAADILVNFF+K KADP+YWDK+S+GGL+RIYEKYTWKLYSER
Sbjct: 12 IIVNCVSGLHIDPYHSDKAADILVNFFEKSKADPTYWDKMSEGGLKRIYEKYTWKLYSER 191
Query: 2357 LMTLTGVYGFWKYVSNLERRETRRYIEMFYALKYRSLASQVPLSFD 2494
LMTLTGVYGFWKYVSNLERRETRRY+EMFYALKYRSLA+ VPL+ D
Sbjct: 192 LMTLTGVYGFWKYVSNLERRETRRYLEMFYALKYRSLAAAVPLAVD 329
Score = 164 bits (352), Expect = 1e-040
Identities = 78/106 (73%), Positives = 83/106 (78%)
Frame = -2 / -3
Query: 2493 SKDSGTWLARLRYFRA*NIXXXXXXXXXXXXLTYFQNPYTPVRVISLSE*SFQVYFS*IL 2314
S +GT ARLRYFRA*NI LTYFQNPYTPVRVISLSE SFQVYFS*IL
Sbjct: 328 STANGTAAARLRYFRA*NISR*RRVSRLSRLLTYFQNPYTPVRVISLSEYSFQVYFS*IL 149
Query: 2313 CRPPCEILSQ*LGSALHLSKKLTRISAALSLW*GSMCRPDTPSTMI 2176
RPP +ILSQ*+GSAL SKKLT+ISAALSLW*GSMC+PDT TMI
Sbjct: 148 LRPPSDILSQ*VGSALDFSKKLTKISAALSLW*GSMCKPDTQFTMI 11
Score = 144 bits (310), Expect = 6e-035
Identities = 62/106 (58%), Positives = 76/106 (71%)
Frame = -1 / -2
Query: 2494 IEGQRNLACQATVLQGVEHFDVAAGLAPLQVAHVLPEPVHAGQGHQPLGVELPGVLLIDS 2315
++ Q N QA VLQ VEH VAA L PLQV HV PE ++ GQGHQPL V+LPGVLLI+S
Sbjct: 329 VDSQWNGGRQAAVLQSVEHLQVAASLTPLQVTHVFPESIYTGQGHQPLRVQLPGVLLINS 150
Query: 2314 LQAAL*DLVPVARICLAFVKEVDQDIRGLVAVVRVNVQARYPVHDD 2177
L+A+ VPV + L +KEVDQDI LV VVR++VQ R+ +HDD
Sbjct: 149 LEASFRHFVPVGWVRLGLLKEVDQDICSLVTVVRIDVQTRHTIHDD 12
Score = 133 bits (284), Expect = 2e-031
Identities = 62/98 (63%), Positives = 72/98 (73%)
Frame = +1 / +2
Query: 2176 DHRGRGIWPAH*PLPQRQGRGYPGQLL*QMQGRSELLGQDLTGRPAENL*EVHLEALLRE 2355
DHR +W AH LPQ QG Y GQLL*++QG LLGQ++ RP ENL*EVHLEA+L E
Sbjct: 11 DHRELCVWFAHRSLPQ*QGCRYLGQLL*EVQGGPNLLGQNVGRRPQENL*EVHLEAVLGE 190
Query: 2356 ADDPDRRVRVLEVREQPGEARDPPLHRNVLRPEVP*PG 2469
ADDPDR + +LE+RE PGEA D PL +VLR EVP PG
Sbjct: 191 ADDPDRCIWILEIRE*PGEA*DSPLPGDVLRSEVPQPG 304
Score = 111 bits (236), Expect = 1e-024
Identities = 51/84 (60%), Positives = 60/84 (71%)
Frame = +3 / +1
Query: 2229 RPRISWSTSLTNARQIRATGTRSHRAACRESMRSTPGSSTPRG**P*PACTGSGST*ATW 2408
R +ISWSTSL + R+ + TGT+ + A RE MRSTPGS T RG**P*P SG+T* TW
Sbjct: 64 RLQISWSTSLRSPRRTQPTGTKCRKEASREFMRSTPGSCTRRG**P*PVYMDSGNT*VTW 243
Query: 2409 RGARPAATSKCSTP*STVAWQARF 2480
RG R AAT +CST *ST AW+ F
Sbjct: 244 RGVRLAATWRCSTL*STAAWRPPF 315
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 39,810,792
Number of Sequences: 8143
Number of extensions: 634195
Number of successful extensions: 3336
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 47
effective length of database: 1,184,766
effective search space used: 1303242600
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)