TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.202
(3202 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815444.1|CN815444 HRO4509_G04_M07ZS5 Lib AA071E1X Aven... 213 1e-055
>gb|CN815444.1|CN815444 HRO4509_G04_M07ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_G04_M07, mRNA sequence
Length = 630
Score = 213 bits (460), Expect = 1e-055
Identities = 84/114 (73%), Positives = 101/114 (88%)
Frame = -2 / +3
Query: 864 IIVHGVSGYHIDPYQGDKASALLVDFFDKCQAEPSHWSKISQGGLQRIEEKYTWKLYSER 685
IIV+ VSG HIDPY DKA+ +LV+FF+K +A+P++W K+S+GGL+RI EKYTWKLYSER
Sbjct: 12 IIVNCVSGLHIDPYHSDKAADILVNFFEKSKADPTYWDKMSEGGLKRIYEKYTWKLYSER 191
Query: 684 LMTLTGVYGFWKYVSNLERRETRRYLEMLYALKYRTMASTVPLAVEGEPSSK*C 523
LMTLTGVYGFWKYVSNLERRETRRYLEM YALKYR++A+ VPLAV+GE + *C
Sbjct: 192 LMTLTGVYGFWKYVSNLERRETRRYLEMFYALKYRSLAAAVPLAVDGESTDN*C 353
Score = 108 bits (231), Expect(2) = 9e-031
Identities = 52/74 (70%), Positives = 55/74 (74%)
Frame = +2 / -3
Query: 644 TYFQNPYTPVRVISLSEYSFQVYFSSIRWSPPWEILLQWLGSAWHLSKKSTSRADALSPW 823
TYFQNPYTPVRVISLSEYSFQVYFS I PP +IL Q +GSA SKK T + ALS W
Sbjct: 232 TYFQNPYTPVRVISLSEYSFQVYFS*ILLRPPSDILSQ*VGSALDFSKKLTKISAALSLW 53
Query: 824 *GSMW*PDTPCTMI 865
*GSM PDT TMI
Sbjct: 52 *GSMCKPDTQFTMI 11
Score = 99.6 bits (211), Expect(2) = 1e-025
Identities = 40/70 (57%), Positives = 52/70 (74%)
Frame = +1 / -2
Query: 523 ASLAGGLSLHGQRHGARHGAVLERVQHLQVPPGLAPLQVGHVLPEPVHAGEGHQPLRVQL 702
A + G L++ Q +G R AVL+ V+HLQV L PLQV HV PE ++ G+GHQPLRVQL
Sbjct: 353 ALIVGALAVDSQWNGGRQAAVLQSVEHLQVAASLTPLQVTHVFPESIYTGQGHQPLRVQL 174
Query: 703 PGVLLLDTLE 732
PGVLL+++LE
Sbjct: 173 PGVLLINSLE 144
Score = 98.7 bits (209), Expect = 5e-021
Identities = 48/95 (50%), Positives = 58/95 (61%)
Frame = -1 / +2
Query: 865 DHRARRVWLPHRPLPGRQGVGPARGLLRQVPGGAEPLEQDLPGRAPAYRGEVHLEAVLGE 686
DHR VW HR LP QG LL +V GG L Q++ R EVHLEAVLGE
Sbjct: 11 DHRELCVWFAHRSLPQ*QGCRYLGQLL*EVQGGPNLLGQNVGRRPQENL*EVHLEAVLGE 190
Query: 685 ADDPHRRVRVLEVRVQPGEARDPAVPGDAVRAQVP 581
ADDP R + +LE+R PGEA D +PGD +R++VP
Sbjct: 191 ADDPDRCIWILEIRE*PGEA*DSPLPGDVLRSEVP 295
Score = 41.8 bits (85), Expect(2) = 9e-031
Identities = 19/41 (46%), Positives = 23/41 (56%)
Frame = +3 / -1
Query: 519 SRITCWRALPPRPAARCSPWCGT*ARTASPGTAGSRASPGW 641
SRI C + +P R P CGT R SPG+ S ASPG+
Sbjct: 357 SRINCRCSRRRQPMERRPPGCGTSERRTSPGSGESHASPGY 235
Score = 34.1 bits (68), Expect(2) = 1e-025
Identities = 15/33 (45%), Positives = 17/33 (51%)
Frame = +3 / -1
Query: 750 CSSGSAPPGTCRRSPRAGPTPCRPGKGRCGSQT 848
C S PP T +RS PC GK RC +QT
Sbjct: 126 CPSRLGPPWTSQRS*PRYLQPCHCGKDRCANQT 28
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 37,920,276
Number of Sequences: 8143
Number of extensions: 627665
Number of successful extensions: 4280
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 47
effective length of database: 1,184,766
effective search space used: 1207276554
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)