TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.149
(1197 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815339.1|CN815339 HRO4508_F01_L02ZS5 Lib AA071E1X Aven... 275 4e-082
gb|AF117889.1|AF117889 Avena fatua 40S ribosomal protein S3... 153 5e-038
gb|CN821129.1|CN821129 HRO4427_G12_N23ZS5 Lib AA069E1X Aven... 127 5e-030
gb|CN816113.1|CN816113 HRO4517_B05_C09ZS5 Lib AA071E1X Aven... 117 3e-027
>gb|CN815339.1|CN815339 HRO4508_F01_L02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_F01_L02, mRNA sequence
Length = 525
Score = 275 bits (596), Expect(2) = 4e-082
Identities = 112/124 (90%), Positives = 121/124 (97%)
Frame = -3 / +1
Query: 745 KKWQTLIEAHVDVKTTDNYMLRLFCIGFTKRRPNQVKRTCYAQASQIRQIRRKMVEIMIN 566
KKWQTLIEAHVDVKTTDNYMLRLFCIGFTKRRPNQVKRTCYAQASQIRQIRRKMVEIM N
Sbjct: 25 KKWQTLIEAHVDVKTTDNYMLRLFCIGFTKRRPNQVKRTCYAQASQIRQIRRKMVEIMAN 204
Query: 565 QASTCDLKELVSKFIPEVIGKEIEKSTSSIFPLQNVFIRKVKILKAPKFDLGKLMEVHGD 386
ASTCDLKELV+KFIPEVIGK+IEK+TSSIFPLQNV++RKVKILK+PKFD+GKLMEVHGD
Sbjct: 205 HASTCDLKELVAKFIPEVIGKDIEKATSSIFPLQNVYVRKVKILKSPKFDIGKLMEVHGD 384
Query: 385 YKED 374
Y ++
Sbjct: 385 YNKE 396
Score = 168 bits (361), Expect(2) = 6e-046
Identities = 76/109 (69%), Positives = 81/109 (74%)
Frame = +3 / -2
Query: 417 SNLGALSIFTLRMKTF*SGKMLEVDFSISLPITSGMNFETSSFRSHVEAWLXXXXXXXXX 596
SN G IFTLR TF SGKMLEV FSIS P+TSG+NF TSSF+S VEAWL
Sbjct: 353 SNFGDFRIFTLRTYTFWSGKMLEVAFSISFPMTSGINFATSSFKSQVEAWLAMISTIFRR 174
Query: 597 XXXXWDA*A*QVLLTWFGRLLVKPMQNKRNM*LSVVLTSTWASINVCHF 743
W A*A*QV TWFGRLLVKPMQN R+M*LSVVLTSTWASI+VCHF
Sbjct: 173 ICLIWLA*A*QVRFTWFGRLLVKPMQNSRSM*LSVVLTSTWASISVCHF 27
Score = 143 bits (306), Expect = 7e-035
Identities = 64/123 (52%), Positives = 89/123 (72%)
Frame = +2 / -1
Query: 374 ILFVVAMNLHELPKIELGGLEYLHLADEDVLKWEDAGGRLLNLLTNNFWNELRNKFFQVT 553
+L VV + LH+LP IEL L+ L+LAD +L+WE+A LLN+ +N+ ++ N+ QVT
Sbjct: 396 LLVVVTVYLHQLPNIELW*LQDLYLADIHILEWENA*SCLLNIFSNDLRDKFCNQLLQVT 217
Query: 554 CRSLVDHNLNHLTADLTDLGCLSIAGSLDLVWAPLGEADAEQTQHVVVGCLDINMGFD*C 733
S+V H+L+HL A+L+DL LSIA +L LVW L EA+AEQ +HV+V L+INMG +*C
Sbjct: 216 G*SVVGHDLDHLPANLSDLAGLSIASTLHLVWTSLSEANAEQPEHVIVSSLNINMGLN*C 37
Query: 734 LPL 742
LPL
Sbjct: 36 LPL 28
Score = 45.1 bits (92), Expect(2) = 4e-082
Identities = 20/33 (60%), Positives = 22/33 (66%)
Frame = -3 / +1
Query: 382 KEDVGVKLERPVEGDEAGQEVAAAE*FVCYFYI 284
KED+GVKL+RP EGDEA AE* C F I
Sbjct: 391 KEDIGVKLDRPAEGDEAMATEVTAE*SWCCFLI 489
Score = 31.8 bits (63), Expect(2) = 6e-046
Identities = 16/36 (44%), Positives = 21/36 (58%)
Frame = +2 / -1
Query: 281 LDIEVANKLLGSSNLLPSLISLDRPLKLHTNILFVV 388
L+ E LL + LIS RP++LHT+IL VV
Sbjct: 492 LN*ETTPTLLSCNLRCHRLISFSRPIQLHTDILLVV 385
>gb|AF117889.1|AF117889 Avena fatua 40S ribosomal protein S3A mRNA, partial cds
Length = 382
Score = 153 bits (329), Expect = 5e-038
Identities = 62/72 (86%), Positives = 68/72 (94%)
Frame = -3 / +2
Query: 550 DLKELVSKFIPEVIGKEIEKSTSSIFPLQNVFIRKVKILKAPKFDLGKLMEVHGDYKEDV 371
DLKELV KFIPE+IGKEIEK+T SIFPLQNV++RKVKILKAPKFDLGKLMEVHGDYKEDV
Sbjct: 2 DLKELVQKFIPELIGKEIEKATPSIFPLQNVYVRKVKILKAPKFDLGKLMEVHGDYKEDV 181
Query: 370 GVKLERPVEGDE 335
G KL+RP +GDE
Sbjct: 182 GTKLDRPADGDE 217
>gb|CN821129.1|CN821129 HRO4427_G12_N23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_G12_N23, mRNA
sequence
Length = 497
Score = 127 bits (271), Expect = 5e-030
Identities = 50/58 (86%), Positives = 56/58 (96%)
Frame = -3 / +3
Query: 508 GKEIEKSTSSIFPLQNVFIRKVKILKAPKFDLGKLMEVHGDYKEDVGVKLERPVEGDE 335
GKEIEK+T+SIFPLQNV++RKVKILKAPKFDLGKLMEVHGDYKEDVG KL+RP +GDE
Sbjct: 12 GKEIEKATASIFPLQNVYVRKVKILKAPKFDLGKLMEVHGDYKEDVGTKLDRPADGDE 185
>gb|CN816113.1|CN816113 HRO4517_B05_C09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_B05_C09, mRNA sequence
Length = 846
Score = 117 bits (251), Expect = 3e-027
Identities = 46/54 (85%), Positives = 52/54 (96%)
Frame = -3 / +2
Query: 496 EKSTSSIFPLQNVFIRKVKILKAPKFDLGKLMEVHGDYKEDVGVKLERPVEGDE 335
EK+T+SIFPLQNV++RKVKILKAPKFDLGKLMEVHGDYKEDVG KL+RP +GDE
Sbjct: 539 EKATASIFPLQNVYVRKVKILKAPKFDLGKLMEVHGDYKEDVGTKLDRPADGDE 700
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,292,608
Number of Sequences: 8143
Number of extensions: 192589
Number of successful extensions: 1267
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 428055030
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)