TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.130
(860 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821219.1|CN821219 HRO4428_H01_P02ZS5 Lib AA069E1X Aven... 257 2e-069
gb|CN816185.1|CN816185 HRO4517_H07_O13ZS5 Lib AA071E1X Aven... 161 1e-040
gb|CN819635.1|CN819635 HRO4402_D11_G22ZS5 Lib AA069E1X Aven... 140 3e-034
>gb|CN821219.1|CN821219 HRO4428_H01_P02ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4428_H01_P02, mRNA
sequence
Length = 576
Score = 257 bits (556), Expect = 2e-069
Identities = 105/110 (95%), Positives = 110/110 (100%)
Frame = +3 / +3
Query: 222 FPARARRRFQRGLKRKPMALIKKLRKAKKDAPAGEKPEPVKTHLRNMIIVPEMIGSIVGV 401
FPARARRRF+RGLKRKPMAL+KKLRKAKKDAPAGEKPEPV+THLRNMIIVPEMIGS++GV
Sbjct: 12 FPARARRRFKRGLKRKPMALVKKLRKAKKDAPAGEKPEPVRTHLRNMIIVPEMIGSLIGV 191
Query: 402 YNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK* 551
YNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK*
Sbjct: 192 YNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK* 341
Score = 234 bits (505), Expect = 2e-062
Identities = 100/109 (91%), Positives = 101/109 (92%)
Frame = -2 / -3
Query: 550 HFRGINREEWVAPIPGLPCLTGL*EMENSAR*WPIISGLISTWLKVLPL*TPTMLPIISG 371
H RG+NREEWVAPIPGLPC TGL*EMENSAR*WPIISGLISTWLKVLPL*TP LPIISG
Sbjct: 340 HLRGMNREEWVAPIPGLPCFTGL*EMENSAR*WPIISGLISTWLKVLPL*TPIRLPIISG 161
Query: 370 TMIMLRRCVLTGSGFSPAGASFFALRSFLMSAMGFLFRPLWNLLLARAG 224
TMIMLRRCVLTGSGFSPAGASFFALRSFL SAMGFL RPL NLL ARAG
Sbjct: 160 TMIMLRRCVLTGSGFSPAGASFFALRSFLTSAMGFLLRPLLNLLRARAG 14
Score = 206 bits (444), Expect = 5e-054
Identities = 86/111 (77%), Positives = 97/111 (87%)
Frame = -1 / -2
Query: 554 SSFQRNKPRGVGGTDTGPAVLDRLVGDGELSKVVADHLRLDLNLVEGLAVVDTNNASNHL 375
+SF+R++P GVGGTDTGP +L RLVGDGELSKV+A+HLRLDLNLVEGLA+VDT+ A+NHL
Sbjct: 344 NSFERDEPGGVGGTDTGPTMLHRLVGDGELSKVMANHLRLDLNLVEGLAIVDTDKAANHL 165
Query: 374 RDNDHVTEMCLDWLRLLTSRSILFRLAQFLDERHGLPLQTPLEPSPGARGE 222
RDNDHV EMC LRLLTSR ILFRLAQ LDERHGLPL+T LEPS G GE
Sbjct: 164 RDNDHVAEMCPHRLRLLTSRGILFRLAQLLDERHGLPLETSLEPSAGTGGE 12
Score = 193 bits (415), Expect = 5e-050
Identities = 81/106 (76%), Positives = 89/106 (83%)
Frame = +2 / +2
Query: 239 EKVPEGSEEEAHGAHQETAQGEKGCSCW*EA*ASQDTSP*HDHCP*DDWKHCWCLQRQDL 418
+KV E S+EEAHGA QE AQGEKGC CW*EA*A +DTSP HDHCP*DDW+ CLQ QDL
Sbjct: 29 QKVQERSQEEAHGARQEAAQGEKGCPCW*EA*ACEDTSPQHDHCP*DDWQPYRCLQWQDL 208
Query: 419 QPG*DQA*DDRPLPC*VLHLLQAGQARQARYRCHPLLAVYSSEMSW 556
QPG*DQA*DD PLPC*VLHLLQA +A ARYRCHPLL V+ S+MS+
Sbjct: 209 QPG*DQA*DDWPLPC*VLHLLQACEAW*ARYRCHPLLPVHPSQMSY 346
>gb|CN816185.1|CN816185 HRO4517_H07_O13ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_H07_O13, mRNA sequence
Length = 416
Score = 161 bits (347), Expect = 1e-040
Identities = 64/71 (90%), Positives = 69/71 (97%)
Frame = +3 / +2
Query: 339 VKTHLRNMIIVPEMIGSIVGVYNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGA 518
V+THLRNMII+PEMIGS+VG+YNGK FN VEIKPEMIGHYLAEFS+SYKPVKHGRPGIGA
Sbjct: 11 VRTHLRNMIIMPEMIGSVVGIYNGKAFNSVEIKPEMIGHYLAEFSLSYKPVKHGRPGIGA 190
Query: 519 THSSRFIPLK* 551
THSSRFIPLK*
Sbjct: 191 THSSRFIPLK* 223
Score = 137 bits (293), Expect = 3e-033
Identities = 60/72 (83%), Positives = 62/72 (86%)
Frame = -2 / -3
Query: 553 AHFRGINREEWVAPIPGLPCLTGL*EMENSAR*WPIISGLISTWLKVLPL*TPTMLPIIS 374
+H G+N EEWVAPIPGLPCLTGL*E ENSAR*WPIISGLIST LK LPL* PT LPIIS
Sbjct: 225 SHLSGMNLEEWVAPIPGLPCLTGL*ERENSAR*WPIISGLISTELKALPL*IPTTLPIIS 46
Query: 373 GTMIMLRRCVLT 338
G MIMLRR VLT
Sbjct: 45 GMMIMLRRWVLT 10
Score = 110 bits (234), Expect = 4e-025
Identities = 47/68 (69%), Positives = 59/68 (86%)
Frame = -1 / -2
Query: 551 SFQRNKPRGVGGTDTGPAVLDRLVGDGELSKVVADHLRLDLNLVEGLAVVDTNNASNHLR 372
S + N+P GVGGT+T P+VLD LVG+GEL +VVA+HLRLDL+ VEGLAVVDT++ASNHLR
Sbjct: 223 SLEWNEP*GVGGTNTRPSVLDWLVGEGELCQVVANHLRLDLDGVEGLAVVDTDDASNHLR 44
Query: 371 DNDHVTEM 348
+DHV E+
Sbjct: 43 HDDHVAEV 20
>gb|CN819635.1|CN819635 HRO4402_D11_G22ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4402_D11_G22, mRNA
sequence
Length = 354
Score = 140 bits (300), Expect = 3e-034
Identities = 55/61 (90%), Positives = 59/61 (96%)
Frame = +3 / +3
Query: 369 VPEMIGSIVGVYNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK 548
+PEMIGS+VG+YNGK FN VEIKPEMIGHYLAEFS+SYKPVKHGRPGIGATHSSRFIPLK
Sbjct: 6 MPEMIGSVVGIYNGKAFNSVEIKPEMIGHYLAEFSLSYKPVKHGRPGIGATHSSRFIPLK 185
Query: 549 * 551
*
Sbjct: 186 * 188
Score = 119 bits (255), Expect = 5e-028
Identities = 51/61 (83%), Positives = 53/61 (86%)
Frame = -2 / -3
Query: 553 AHFRGINREEWVAPIPGLPCLTGL*EMENSAR*WPIISGLISTWLKVLPL*TPTMLPIIS 374
+H G+N EEWVAPIPGLPCLTGL*E ENSAR*WPIISGLIST LK LPL* PT LPIIS
Sbjct: 190 SHLSGMNLEEWVAPIPGLPCLTGL*ERENSAR*WPIISGLISTELKALPL*IPTTLPIIS 11
Query: 373 G 371
G
Sbjct: 10 G 8
Score = 99.1 bits (210), Expect = 9e-022
Identities = 43/60 (71%), Positives = 53/60 (88%)
Frame = -1 / -2
Query: 551 SFQRNKPRGVGGTDTGPAVLDRLVGDGELSKVVADHLRLDLNLVEGLAVVDTNNASNHLR 372
S + N+P GVGGT+T P+VLD LVG+GEL +VVA+HLRLDL+ VEGLAVVDT++ASNHLR
Sbjct: 188 SLEWNEP*GVGGTNTRPSVLDWLVGEGELCQVVANHLRLDLDGVEGLAVVDTDDASNHLR 9
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,140,393
Number of Sequences: 8143
Number of extensions: 102792
Number of successful extensions: 741
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 295813134
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)