TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBD3a09.xg.3.1
(1105 letters)
Database: At_nucl_with_EST.fasta
1,013,581 sequences; 908,940,872 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BT002376.1| Arabidopsis thaliana unknown protein (At1g17... 143 3e-069
gb|BT009671.1| Arabidopsis thaliana At1g17330 mRNA, complet... 143 3e-069
ref|NM_101594.1| Arabidopsis thaliana catalytic AT1G17330 m... 143 3e-069
emb|BX818451.1|CNS0AE1F Arabidopsis thaliana Full-length cD... 115 4e-050
gb|AE005172.1| Arabidopsis thaliana chromosome 1, top arm c... 72 3e-043
gb|AC007843.6|AC007843 Arabidopsis thaliana chromosome I BA... 72 3e-043
>gb|BT002376.1| Arabidopsis thaliana unknown protein (At1g17330) mRNA, complete cds
Length = 935
Score = 143 bits (306), Expect(4) = 3e-069
Identities = 57/99 (57%), Positives = 77/99 (77%)
Frame = +1 / +1
Query: 355 VVEMFLQEVGLDEGQKDEIVAIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIGAIG 534
+VE FL + G++E +K +I+ II GMGFK+E++ ++ E EF +VQDADRLDAIGAIG
Sbjct: 289 LVENFLDDEGIEETKKTKILTIINGMGFKDELAGVALCESLPEFGVVQDADRLDAIGAIG 468
Query: 535 IARCFTYGGSKNSALHDPRILPRDSLSKEKYMSKEEKQT 651
IARCFT+GGS+N LHDP I PR L+KE+Y+ +EE+ T
Sbjct: 469 IARCFTFGGSRNRVLHDPEIKPRTELTKEQYIKREEQTT 585
Score = 106 bits (226), Expect(4) = 3e-069
Identities = 40/51 (78%), Positives = 46/51 (90%)
Frame = +1 / +1
Query: 634 KEEKQTSINHFHEKLFKLKDMMKTEAGKRRAEKRHRFMEEFVAEFYEEWSG 786
K E+QT+INHFHEKL KLK +MKTEAGKRRAEKRH+FMEE++ EFYEEW G
Sbjct: 565 KREEQTTINHFHEKLLKLKKLMKTEAGKRRAEKRHKFMEEYLKEFYEEWDG 717
Score = 91.8 bits (194), Expect(3) = 2e-032
Identities = 54/102 (52%), Positives = 60/102 (58%)
Frame = -3 / -1
Query: 650 VCFSSLDMYFSFDKLSRGRILGSCNALFLLPPYVKHLAIPIAPIASRRSASCTIANSRVG 471
V SSL MY S RG I GS + LFLLPP VK AIP+APIASRRSAS T NS
Sbjct: 584 VVCSSLLMYCSLVSSVRGLISGS*STLFLLPPNVKQRAIPMAPIASRRSAS*TTPNSGRD 405
Query: 470 STIDLFETSFLNPIPLIIATISSFWPSSNPTS*RNISTTLIS 345
S S L PIPL+I +I F SS P+S R ST+ S
Sbjct: 404 SQSATPANSSLKPIPLMIVSIFVFLVSSIPSSSRKFSTSFSS 279
Score = 44.6 bits (91), Expect(3) = 2e-032
Identities = 20/47 (42%), Positives = 27/47 (57%)
Frame = -2 / -3
Query: 795 SSPATPLFVEFSHKFLHEPMPFLSSPFPGLCLHHVLQFKKLLMKMID 655
SS PLF+E HE +PFL PF L H +L ++ LMK+I+
Sbjct: 726 SS*PIPLFIELLKILFHELVPFLCPPFTRLSFHQLL*LEEFLMKVIN 586
Score = 43.7 bits (89), Expect(3) = 2e-032
Identities = 21/43 (48%), Positives = 22/43 (51%)
Frame = -2 / -3
Query: 258 EALFGCERERKVSDAERVRGVVGGIAPAHLPLHQLLRSPHRRR 130
E E KVSD V GV+ I H LHQLLR PHR R
Sbjct: 192 ETFLASNGESKVSDPPHVPGVMRSIVSFHRLLHQLLRLPHRLR 64
Score = 42.3 bits (86), Expect(4) = 3e-069
Identities = 17/30 (56%), Positives = 22/30 (73%)
Frame = +1 / +1
Query: 268 DRLLTVELAALLHDVGDYKYTKNNVEDMSV 357
D + VELAALLHD+GDYKY ++ E+ V
Sbjct: 205 DSMEIVELAALLHDIGDYKYIRDPSEEKLV 294
Score = 30.9 bits (61), Expect(4) = 3e-069
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = +1 / +1
Query: 178 GRDASHDAAHALRVRD 225
G DASHDA H RVRD
Sbjct: 112 GNDASHDAWHVWRVRD 159
>gb|BT009671.1| Arabidopsis thaliana At1g17330 mRNA, complete cds
Length = 669
Score = 143 bits (306), Expect(4) = 3e-069
Identities = 57/99 (57%), Positives = 77/99 (77%)
Frame = +1 / +1
Query: 355 VVEMFLQEVGLDEGQKDEIVAIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIGAIG 534
+VE FL + G++E +K +I+ II GMGFK+E++ ++ E EF +VQDADRLDAIGAIG
Sbjct: 232 LVENFLDDEGIEETKKTKILTIINGMGFKDELAGVALCESLPEFGVVQDADRLDAIGAIG 411
Query: 535 IARCFTYGGSKNSALHDPRILPRDSLSKEKYMSKEEKQT 651
IARCFT+GGS+N LHDP I PR L+KE+Y+ +EE+ T
Sbjct: 412 IARCFTFGGSRNRVLHDPEIKPRTELTKEQYIKREEQTT 528
Score = 106 bits (226), Expect(4) = 3e-069
Identities = 40/51 (78%), Positives = 46/51 (90%)
Frame = +1 / +1
Query: 634 KEEKQTSINHFHEKLFKLKDMMKTEAGKRRAEKRHRFMEEFVAEFYEEWSG 786
K E+QT+INHFHEKL KLK +MKTEAGKRRAEKRH+FMEE++ EFYEEW G
Sbjct: 508 KREEQTTINHFHEKLLKLKKLMKTEAGKRRAEKRHKFMEEYLKEFYEEWDG 660
Score = 91.8 bits (194), Expect(3) = 2e-032
Identities = 54/102 (52%), Positives = 60/102 (58%)
Frame = -3 / -2
Query: 650 VCFSSLDMYFSFDKLSRGRILGSCNALFLLPPYVKHLAIPIAPIASRRSASCTIANSRVG 471
V SSL MY S RG I GS + LFLLPP VK AIP+APIASRRSAS T NS
Sbjct: 527 VVCSSLLMYCSLVSSVRGLISGS*STLFLLPPNVKQRAIPMAPIASRRSAS*TTPNSGRD 348
Query: 470 STIDLFETSFLNPIPLIIATISSFWPSSNPTS*RNISTTLIS 345
S S L PIPL+I +I F SS P+S R ST+ S
Sbjct: 347 SQSATPANSSLKPIPLMIVSIFVFLVSSIPSSSRKFSTSFSS 222
Score = 44.6 bits (91), Expect(3) = 2e-032
Identities = 20/47 (42%), Positives = 27/47 (57%)
Frame = -2 / -1
Query: 795 SSPATPLFVEFSHKFLHEPMPFLSSPFPGLCLHHVLQFKKLLMKMID 655
SS PLF+E HE +PFL PF L H +L ++ LMK+I+
Sbjct: 669 SS*PIPLFIELLKILFHELVPFLCPPFTRLSFHQLL*LEEFLMKVIN 529
Score = 43.7 bits (89), Expect(3) = 2e-032
Identities = 21/43 (48%), Positives = 22/43 (51%)
Frame = -2 / -1
Query: 258 EALFGCERERKVSDAERVRGVVGGIAPAHLPLHQLLRSPHRRR 130
E E KVSD V GV+ I H LHQLLR PHR R
Sbjct: 135 ETFLASNGESKVSDPPHVPGVMRSIVSFHRLLHQLLRLPHRLR 7
Score = 42.3 bits (86), Expect(4) = 3e-069
Identities = 17/30 (56%), Positives = 22/30 (73%)
Frame = +1 / +1
Query: 268 DRLLTVELAALLHDVGDYKYTKNNVEDMSV 357
D + VELAALLHD+GDYKY ++ E+ V
Sbjct: 148 DSMEIVELAALLHDIGDYKYIRDPSEEKLV 237
Score = 30.9 bits (61), Expect(4) = 3e-069
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = +1 / +1
Query: 178 GRDASHDAAHALRVRD 225
G DASHDA H RVRD
Sbjct: 55 GNDASHDAWHVWRVRD 102
>ref|NM_101594.1| Arabidopsis thaliana catalytic AT1G17330 mRNA, complete cds
Length = 669
Score = 143 bits (306), Expect(4) = 3e-069
Identities = 57/99 (57%), Positives = 77/99 (77%)
Frame = +1 / +1
Query: 355 VVEMFLQEVGLDEGQKDEIVAIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIGAIG 534
+VE FL + G++E +K +I+ II GMGFK+E++ ++ E EF +VQDADRLDAIGAIG
Sbjct: 232 LVENFLDDEGIEETKKTKILTIINGMGFKDELAGVALCESLPEFGVVQDADRLDAIGAIG 411
Query: 535 IARCFTYGGSKNSALHDPRILPRDSLSKEKYMSKEEKQT 651
IARCFT+GGS+N LHDP I PR L+KE+Y+ +EE+ T
Sbjct: 412 IARCFTFGGSRNRVLHDPEIKPRTELTKEQYIKREEQTT 528
Score = 106 bits (226), Expect(4) = 3e-069
Identities = 40/51 (78%), Positives = 46/51 (90%)
Frame = +1 / +1
Query: 634 KEEKQTSINHFHEKLFKLKDMMKTEAGKRRAEKRHRFMEEFVAEFYEEWSG 786
K E+QT+INHFHEKL KLK +MKTEAGKRRAEKRH+FMEE++ EFYEEW G
Sbjct: 508 KREEQTTINHFHEKLLKLKKLMKTEAGKRRAEKRHKFMEEYLKEFYEEWDG 660
Score = 91.8 bits (194), Expect(3) = 2e-032
Identities = 54/102 (52%), Positives = 60/102 (58%)
Frame = -3 / -2
Query: 650 VCFSSLDMYFSFDKLSRGRILGSCNALFLLPPYVKHLAIPIAPIASRRSASCTIANSRVG 471
V SSL MY S RG I GS + LFLLPP VK AIP+APIASRRSAS T NS
Sbjct: 527 VVCSSLLMYCSLVSSVRGLISGS*STLFLLPPNVKQRAIPMAPIASRRSAS*TTPNSGRD 348
Query: 470 STIDLFETSFLNPIPLIIATISSFWPSSNPTS*RNISTTLIS 345
S S L PIPL+I +I F SS P+S R ST+ S
Sbjct: 347 SQSATPANSSLKPIPLMIVSIFVFLVSSIPSSSRKFSTSFSS 222
Score = 44.6 bits (91), Expect(3) = 2e-032
Identities = 20/47 (42%), Positives = 27/47 (57%)
Frame = -2 / -1
Query: 795 SSPATPLFVEFSHKFLHEPMPFLSSPFPGLCLHHVLQFKKLLMKMID 655
SS PLF+E HE +PFL PF L H +L ++ LMK+I+
Sbjct: 669 SS*PIPLFIELLKILFHELVPFLCPPFTRLSFHQLL*LEEFLMKVIN 529
Score = 43.7 bits (89), Expect(3) = 2e-032
Identities = 21/43 (48%), Positives = 22/43 (51%)
Frame = -2 / -1
Query: 258 EALFGCERERKVSDAERVRGVVGGIAPAHLPLHQLLRSPHRRR 130
E E KVSD V GV+ I H LHQLLR PHR R
Sbjct: 135 ETFLASNGESKVSDPPHVPGVMRSIVSFHRLLHQLLRLPHRLR 7
Score = 42.3 bits (86), Expect(4) = 3e-069
Identities = 17/30 (56%), Positives = 22/30 (73%)
Frame = +1 / +1
Query: 268 DRLLTVELAALLHDVGDYKYTKNNVEDMSV 357
D + VELAALLHD+GDYKY ++ E+ V
Sbjct: 148 DSMEIVELAALLHDIGDYKYIRDPSEEKLV 237
Score = 30.9 bits (61), Expect(4) = 3e-069
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = +1 / +1
Query: 178 GRDASHDAAHALRVRD 225
G DASHDA H RVRD
Sbjct: 55 GNDASHDAWHVWRVRD 102
>emb|BX818451.1|CNS0AE1F Arabidopsis thaliana Full-length cDNA Complete sequence from clone
GSLTSIL79ZG09 of Silique of strain col-0 of Arabidopsis
thaliana (thale cress)
Length = 545
Score = 115 bits (245), Expect(2) = 4e-050
Identities = 45/73 (61%), Positives = 58/73 (79%)
Frame = +1 / +2
Query: 433 GFKNEVSNKSIVEPTLEFAIVQDADRLDAIGAIGIARCFTYGGSKNSALHDPRILPRDSL 612
GFK+E++ ++ E EF +VQDADRLDAIGAIGIARCFT+GGS+N LHDP I PR L
Sbjct: 2 GFKDELAGVALCESLPEFGVVQDADRLDAIGAIGIARCFTFGGSRNRVLHDPEIKPRTEL 181
Query: 613 SKEKYMSKEEKQT 651
+KE+Y+ +EE+ T
Sbjct: 182 TKEQYIKREEQTT 220
Score = 106 bits (226), Expect(2) = 4e-050
Identities = 40/51 (78%), Positives = 46/51 (90%)
Frame = +1 / +2
Query: 634 KEEKQTSINHFHEKLFKLKDMMKTEAGKRRAEKRHRFMEEFVAEFYEEWSG 786
K E+QT+INHFHEKL KLK +MKTEAGKRRAEKRH+FMEE++ EFYEEW G
Sbjct: 200 KREEQTTINHFHEKLLKLKKLMKTEAGKRRAEKRHKFMEEYLKEFYEEWDG 352
>gb|AE005172.1| Arabidopsis thaliana chromosome 1, top arm complete sequence
Length = 14221815
Score = 72.1 bits (151), Expect(4) = 3e-043
Identities = 26/45 (57%), Positives = 35/45 (77%)
Frame = +1 / +1
Query: 517 AIGAIGIARCFTYGGSKNSALHDPRILPRDSLSKEKYMSKEEKQT 651
++ IGIARCFT+GGS+N LHDP I PR L+KE+Y+ +EE+ T
Sbjct: 5931058 SLSVIGIARCFTFGGSRNRVLHDPEIKPRTELTKEQYIKREEQTT 5931192
Score = 60.2 bits (125), Expect(4) = 3e-043
Identities = 21/27 (77%), Positives = 25/27 (92%)
Frame = +1 / +2
Query: 706 EAGKRRAEKRHRFMEEFVAEFYEEWSG 786
+AGKRRAEKRH+FMEE++ EFYEEW G
Sbjct: 5931323 QAGKRRAEKRHKFMEEYLKEFYEEWDG 5931403
Score = 51.9 bits (107), Expect(4) = 3e-043
Identities = 21/34 (61%), Positives = 27/34 (79%)
Frame = +1 / +2
Query: 433 GFKNEVSNKSIVEPTLEFAIVQDADRLDAIGAIG 534
GFK+E++ ++ E EF +VQDADRLDAIGAIG
Sbjct: 5930843 GFKDELAGVALCESLPEFGVVQDADRLDAIGAIG 5930944
Score = 50.1 bits (103), Expect(4) = 3e-043
Identities = 19/25 (76%), Positives = 22/25 (88%)
Frame = +1 / +1
Query: 634 KEEKQTSINHFHEKLFKLKDMMKTE 708
K E+QT+INHFHEKL KLK +MKTE
Sbjct: 5931172 KREEQTTINHFHEKLLKLKKLMKTE 5931246
>gb|AC007843.6|AC007843 Arabidopsis thaliana chromosome I BAC F28G4 genomic sequence, complete
sequence
Length = 84974
Score = 72.1 bits (151), Expect(4) = 3e-043
Identities = 26/45 (57%), Positives = 35/45 (77%)
Frame = +1 / -1
Query: 517 AIGAIGIARCFTYGGSKNSALHDPRILPRDSLSKEKYMSKEEKQT 651
++ IGIARCFT+GGS+N LHDP I PR L+KE+Y+ +EE+ T
Sbjct: 84374 SLSVIGIARCFTFGGSRNRVLHDPEIKPRTELTKEQYIKREEQTT 84240
Score = 60.2 bits (125), Expect(4) = 3e-043
Identities = 21/27 (77%), Positives = 25/27 (92%)
Frame = +1 / -2
Query: 706 EAGKRRAEKRHRFMEEFVAEFYEEWSG 786
+AGKRRAEKRH+FMEE++ EFYEEW G
Sbjct: 84109 QAGKRRAEKRHKFMEEYLKEFYEEWDG 84029
Score = 51.9 bits (107), Expect(4) = 3e-043
Identities = 21/34 (61%), Positives = 27/34 (79%)
Frame = +1 / -2
Query: 433 GFKNEVSNKSIVEPTLEFAIVQDADRLDAIGAIG 534
GFK+E++ ++ E EF +VQDADRLDAIGAIG
Sbjct: 84589 GFKDELAGVALCESLPEFGVVQDADRLDAIGAIG 84488
Score = 50.1 bits (103), Expect(4) = 3e-043
Identities = 19/25 (76%), Positives = 22/25 (88%)
Frame = +1 / -1
Query: 634 KEEKQTSINHFHEKLFKLKDMMKTE 708
K E+QT+INHFHEKL KLK +MKTE
Sbjct: 84260 KREEQTTINHFHEKLLKLKKLMKTE 84186
Database: At_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:09 PM
Number of letters in database: 908,940,872
Number of sequences in database: 1,013,581
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 2,087,975,154
Number of Sequences: 1013581
Number of extensions: 28644647
Number of successful extensions: 184735
Number of sequences better than 1.0e-020: 7
length of database: 302,980,290
effective HSP length: 57
effective length of database: 245,206,173
effective search space used: 76013913630
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)