TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2647895.2.1
(709 letters)
Database: At_nucl_with_EST.fasta
1,013,581 sequences; 908,940,872 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV783910.1|AV783910 AV783910 RAFL5 Arabidopsis thaliana ... 125 1e-027
gb|AF090372.1|AF090372 Arabidopsis thaliana SAM:cycloarteno... 125 1e-027
gb|AY120716.1| Arabidopsis thaliana 24-sterol C-methyltrans... 125 1e-027
gb|AF494289.1| Arabidopsis thaliana cephalopod (CPH) mRNA, ... 125 1e-027
gb|BT000058.1| Arabidopsis thaliana 24-sterol C-methyltrans... 125 1e-027
emb|CQ804624.1| Sequence 1035 from Patent WO2004035798 125 1e-027
gb|AF195648.1| Arabidopsis thaliana sterol methyltransferas... 125 1e-027
gb|BT015538.1| Arabidopsis thaliana At5g13710 mRNA sequence 125 1e-027
ref|NM_121374.2| Arabidopsis thaliana SMT1 (STEROL METHYLTR... 125 1e-027
emb|BX831439.1|CNS0A23U Arabidopsis thaliana Full-length cD... 123 5e-027
emb|BX833706.1|CNS0A26T Arabidopsis thaliana Full-length cD... 123 5e-027
emb|BX830674.1|CNS0A0QH Arabidopsis thaliana Full-length cD... 120 2e-026
>gb|AV783910.1|AV783910 AV783910 RAFL5 Arabidopsis thaliana cDNA clone RAFL05-14-G19 3',
mRNA sequence
Length = 696
Score = 125 bits (268), Expect = 1e-027
Identities = 52/117 (44%), Positives = 72/117 (61%)
Frame = -1 / -1
Query: 634 EXELGDXXXDIXXXRQCVQAXKXXGFEVVFAKDLAQVFPCPWYRVMDPXXFSWAHFQCPR 455
E E+GD DI +C++A K GFEV++ KDLA+ P PWY +D FS + F+
Sbjct: 525 EIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTA 346
Query: 454 PGRIVTRAIVNTLEFLRIAPAGSMGAYNVLMSASDGLLKGGREGIFTATFFVLGRKP 284
GR +T+ +V LE++R+AP GS N L A++GL+ GGR IFT +F L RKP
Sbjct: 345 VGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 175
>gb|AF090372.1|AF090372 Arabidopsis thaliana SAM:cycloartenol-C24-methyltransferase (SMT1)
mRNA, complete cds
Length = 1269
Score = 125 bits (268), Expect = 1e-027
Identities = 52/117 (44%), Positives = 72/117 (61%)
Frame = -1 / +1
Query: 634 EXELGDXXXDIXXXRQCVQAXKXXGFEVVFAKDLAQVFPCPWYRVMDPXXFSWAHFQCPR 455
E E+GD DI +C++A K GFEV++ KDLA+ P PWY +D FS + F+
Sbjct: 697 EIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTA 876
Query: 454 PGRIVTRAIVNTLEFLRIAPAGSMGAYNVLMSASDGLLKGGREGIFTATFFVLGRKP 284
GR +T+ +V LE++R+AP GS N L A++GL+ GGR IFT +F L RKP
Sbjct: 877 VGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 1047
>gb|AY120716.1| Arabidopsis thaliana 24-sterol C-methyltransferase (At5g13710) mRNA,
complete cds
Length = 1245
Score = 125 bits (268), Expect = 1e-027
Identities = 52/117 (44%), Positives = 72/117 (61%)
Frame = -1 / +2
Query: 634 EXELGDXXXDIXXXRQCVQAXKXXGFEVVFAKDLAQVFPCPWYRVMDPXXFSWAHFQCPR 455
E E+GD DI +C++A K GFEV++ KDLA+ P PWY +D FS + F+
Sbjct: 722 EIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTA 901
Query: 454 PGRIVTRAIVNTLEFLRIAPAGSMGAYNVLMSASDGLLKGGREGIFTATFFVLGRKP 284
GR +T+ +V LE++R+AP GS N L A++GL+ GGR IFT +F L RKP
Sbjct: 902 VGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 1072
>gb|AF494289.1| Arabidopsis thaliana cephalopod (CPH) mRNA, complete cds
Length = 1080
Score = 125 bits (268), Expect = 1e-027
Identities = 52/117 (44%), Positives = 72/117 (61%)
Frame = -1 / +1
Query: 634 EXELGDXXXDIXXXRQCVQAXKXXGFEVVFAKDLAQVFPCPWYRVMDPXXFSWAHFQCPR 455
E E+GD DI +C++A K GFEV++ KDLA+ P PWY +D FS + F+
Sbjct: 724 EIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTA 903
Query: 454 PGRIVTRAIVNTLEFLRIAPAGSMGAYNVLMSASDGLLKGGREGIFTATFFVLGRKP 284
GR +T+ +V LE++R+AP GS N L A++GL+ GGR IFT +F L RKP
Sbjct: 904 VGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 1074
>gb|BT000058.1| Arabidopsis thaliana 24-sterol C-methyltransferase (At5g13710) mRNA,
complete cds
Length = 1108
Score = 125 bits (268), Expect = 1e-027
Identities = 52/117 (44%), Positives = 72/117 (61%)
Frame = -1 / +1
Query: 634 EXELGDXXXDIXXXRQCVQAXKXXGFEVVFAKDLAQVFPCPWYRVMDPXXFSWAHFQCPR 455
E E+GD DI +C++A K GFEV++ KDLA+ P PWY +D FS + F+
Sbjct: 655 EIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTA 834
Query: 454 PGRIVTRAIVNTLEFLRIAPAGSMGAYNVLMSASDGLLKGGREGIFTATFFVLGRKP 284
GR +T+ +V LE++R+AP GS N L A++GL+ GGR IFT +F L RKP
Sbjct: 835 VGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 1005
>emb|CQ804624.1| Sequence 1035 from Patent WO2004035798
Length = 1011
Score = 125 bits (268), Expect = 1e-027
Identities = 52/117 (44%), Positives = 72/117 (61%)
Frame = -1 / +1
Query: 634 EXELGDXXXDIXXXRQCVQAXKXXGFEVVFAKDLAQVFPCPWYRVMDPXXFSWAHFQCPR 455
E E+GD DI +C++A K GFEV++ KDLA+ P PWY +D FS + F+
Sbjct: 655 EIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTA 834
Query: 454 PGRIVTRAIVNTLEFLRIAPAGSMGAYNVLMSASDGLLKGGREGIFTATFFVLGRKP 284
GR +T+ +V LE++R+AP GS N L A++GL+ GGR IFT +F L RKP
Sbjct: 835 VGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 1005
>gb|AF195648.1| Arabidopsis thaliana sterol methyltransferase SMT1 (SMT1) mRNA,
complete cds
Length = 1257
Score = 125 bits (268), Expect = 1e-027
Identities = 52/117 (44%), Positives = 72/117 (61%)
Frame = -1 / +3
Query: 634 EXELGDXXXDIXXXRQCVQAXKXXGFEVVFAKDLAQVFPCPWYRVMDPXXFSWAHFQCPR 455
E E+GD DI +C++A K GFEV++ KDLA+ P PWY +D FS + F+
Sbjct: 708 EIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTA 887
Query: 454 PGRIVTRAIVNTLEFLRIAPAGSMGAYNVLMSASDGLLKGGREGIFTATFFVLGRKP 284
GR +T+ +V LE++R+AP GS N L A++GL+ GGR IFT +F L RKP
Sbjct: 888 VGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 1058
>gb|BT015538.1| Arabidopsis thaliana At5g13710 mRNA sequence
Length = 1008
Score = 125 bits (268), Expect = 1e-027
Identities = 52/117 (44%), Positives = 72/117 (61%)
Frame = -1 / +1
Query: 634 EXELGDXXXDIXXXRQCVQAXKXXGFEVVFAKDLAQVFPCPWYRVMDPXXFSWAHFQCPR 455
E E+GD DI +C++A K GFEV++ KDLA+ P PWY +D FS + F+
Sbjct: 652 EIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTA 831
Query: 454 PGRIVTRAIVNTLEFLRIAPAGSMGAYNVLMSASDGLLKGGREGIFTATFFVLGRKP 284
GR +T+ +V LE++R+AP GS N L A++GL+ GGR IFT +F L RKP
Sbjct: 832 VGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 1002
>ref|NM_121374.2| Arabidopsis thaliana SMT1 (STEROL METHYLTRANSFERASE 1) AT5G13710
(SMT1) mRNA, complete cds
Length = 1462
Score = 125 bits (268), Expect = 1e-027
Identities = 52/117 (44%), Positives = 72/117 (61%)
Frame = -1 / +3
Query: 634 EXELGDXXXDIXXXRQCVQAXKXXGFEVVFAKDLAQVFPCPWYRVMDPXXFSWAHFQCPR 455
E E+GD DI +C++A K GFEV++ KDLA+ P PWY +D FS + F+
Sbjct: 753 EIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTA 932
Query: 454 PGRIVTRAIVNTLEFLRIAPAGSMGAYNVLMSASDGLLKGGREGIFTATFFVLGRKP 284
GR +T+ +V LE++R+AP GS N L A++GL+ GGR IFT +F L RKP
Sbjct: 933 VGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 1103
>emb|BX831439.1|CNS0A23U Arabidopsis thaliana Full-length cDNA Complete sequence from clone
GSLTLS90ZE01 of Adult vegetative tissue of strain col-0
of Arabidopsis thaliana (thale cress)
Length = 1198
Score = 123 bits (264), Expect = 5e-027
Identities = 51/117 (43%), Positives = 71/117 (60%)
Frame = -1 / +3
Query: 634 EXELGDXXXDIXXXRQCVQAXKXXGFEVVFAKDLAQVFPCPWYRVMDPXXFSWAHFQCPR 455
E E+GD DI +C++A K GFEV++ KDLA+ P PWY +D FS + F+
Sbjct: 681 EIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTA 860
Query: 454 PGRIVTRAIVNTLEFLRIAPAGSMGAYNVLMSASDGLLKGGREGIFTATFFVLGRKP 284
GR +T+ +V LE++R+AP GS N L A++GL+ GGR IFT +F RKP
Sbjct: 861 VGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMYFFFARKP 1031
>emb|BX833706.1|CNS0A26T Arabidopsis thaliana Full-length cDNA Complete sequence from clone
GSLTSIL72ZA01 of Silique of strain col-0 of Arabidopsis
thaliana (thale cress)
Length = 724
Score = 123 bits (264), Expect = 5e-027
Identities = 52/117 (44%), Positives = 71/117 (60%)
Frame = -1 / +3
Query: 634 EXELGDXXXDIXXXRQCVQAXKXXGFEVVFAKDLAQVFPCPWYRVMDPXXFSWAHFQCPR 455
E E+GD DI +C++A K GFEV++ KDLA+ P PWY +D FS + F+
Sbjct: 171 EIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTA 350
Query: 454 PGRIVTRAIVNTLEFLRIAPAGSMGAYNVLMSASDGLLKGGREGIFTATFFVLGRKP 284
GR +T+ +V LE++R+AP GS N L A++GL+ GGR IFT F L RKP
Sbjct: 351 VGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMHFFLARKP 521
>emb|BX830674.1|CNS0A0QH Arabidopsis thaliana Full-length cDNA Complete sequence from clone
GSLTLS13ZC03 of Adult vegetative tissue of strain col-0
of Arabidopsis thaliana (thale cress)
Length = 1283
Score = 120 bits (257), Expect(2) = 2e-026
Identities = 51/117 (43%), Positives = 71/117 (60%)
Frame = -1 / +1
Query: 634 EXELGDXXXDIXXXRQCVQAXKXXGFEVVFAKDLAQVFPCPWYRVMDPXXFSWAHFQCPR 455
E E+GD I +C++A K GFEV++ KDLA+ P PWY +D FS + F+
Sbjct: 706 EIEIGDGLPYIILTTKCLEALKKEGFEVIWEKDLAKDSPFPWYLPLDKNHFSLSSFRLTA 885
Query: 454 PGRIVTRAIVNTLEFLRIAPAGSMGAYNVLMSASDGLLKGGREGIFTATFFVLGRKP 284
GR +T+ +V LE++R+AP GS N L A++GL+ GGR IFT +F L RKP
Sbjct: 886 VGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKP 1056
Score = 23.5 bits (45), Expect(2) = 2e-026
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = -3 / +1
Query: 140 CAVCWPAGSCSRSSLYLLVSL 78
CA+C + SC+ ++ YL + L
Sbjct: 1216 CALCLKSESCAHATKYLGIFL 1278
Database: At_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:09 PM
Number of letters in database: 908,940,872
Number of sequences in database: 1,013,581
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,269,982,614
Number of Sequences: 1013581
Number of extensions: 19281808
Number of successful extensions: 169850
Number of sequences better than 1.0e-020: 12
length of database: 302,980,290
effective HSP length: 56
effective length of database: 246,219,754
effective search space used: 44073335966
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)