TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493528.2.1
(627 letters)
Database: At_nucl_with_EST.fasta
1,013,581 sequences; 908,940,872 total letters
Score E
Sequences producing significant alignments: (bits) Value
ref|NM_120766.3| Arabidopsis thaliana unknown protein AT5G0... 295 1e-078
emb|BX831519.1|CNS0A0W1 Arabidopsis thaliana Full-length cD... 293 4e-078
emb|BX832407.1|CNS0A0ZV Arabidopsis thaliana Full-length cD... 289 5e-077
gb|CB259439.1|CB259439 50-E9537-013-002-C14-T7R MPIZ-ADIS-0... 233 4e-060
dbj|AP002032.1| Arabidopsis thaliana genomic DNA, chromosom... 116 8e-053
gb|CB259351.1|CB259351 58-E9537-013-002-C16-T7R MPIZ-ADIS-0... 191 2e-047
dbj|BA000015.5| Arabidopsis thaliana DNA, chromosome 5, com... 116 6e-025
ref|NC_003076.4| Arabidopsis thaliana chromosome 5, complet... 116 6e-025
>ref|NM_120766.3| Arabidopsis thaliana unknown protein AT5G06830 mRNA, complete cds
Length = 1819
Score = 295 bits (638), Expect = 1e-078
Identities = 129/176 (73%), Positives = 153/176 (86%)
Frame = -2 / +2
Query: 596 DRSLLLEKEYRNNILDDLLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQQHAPDSL 417
+RS LLE EYRN ILDDL EVK+FL QRL E+RN DT SLQH VQAVSP VLQQ++P+++
Sbjct: 1187 ERSQLLETEYRNKILDDLYEVKAFLNQRLIELRNEDTLSLQHHVQAVSPMVLQQYSPETI 1366
Query: 416 ENMLVEISSAISLLTNQKTLDLIMILNSKRFLDRLVSSLEEKKHHEVKLREGLGDLSVKR 237
E M+V+IS AISLLTN+K+ DLIMILNSKRFLDRLVS LEEKKH EVKLRE L D+ +R
Sbjct: 1367 EPMVVDISMAISLLTNKKSRDLIMILNSKRFLDRLVSELEEKKHREVKLRESLKDVGRRR 1546
Query: 236 MELQNALSSSWPKQEAAITKTRELKKLCESTLSSVFDGRPVYIIGEINTLLSSSVS 69
MELQN+LS+ WPKQEAA++KTRELK+LCE++LSS+FDGRPV I GEINTLL++ VS
Sbjct: 1547 MELQNSLSAIWPKQEAALSKTRELKELCETSLSSMFDGRPVNIRGEINTLLNAGVS 1714
Score = 185 bits (399), Expect = 9e-046
Identities = 93/175 (53%), Positives = 109/175 (62%)
Frame = +1 / -2
Query: 73 TLELSKVLISPIIYTGLPSKTXXXXXXXXXXXXXXXXIAASCFGHDDDSAFCSSIRLTDK 252
T + VLISP+I+TGLPS AASCFGH +S FCSS+RL
Sbjct: 1710 TPAFNNVLISPLIFTGLPSNMEERLVSHSSLSSLVFESAASCFGHIAESEFCSSMRLLPT 1531
Query: 253 SPKPXXXXXXXXXXXXKLETNLSRNLLELRIIIRSRVF*LVSNEIADEISTSMFSKLSGA 432
S K ET+LSRNLLELRI+IRSR F VSNE+A E+ST++ S +SG
Sbjct: 1530 SFKLSLNFTSRCFFSSSSETSLSRNLLELRIMIRSRDFLFVSNEMAMEMSTTIGSMVSGE 1351
Query: 433 CCWRTKGETACT*CCRDDVSAFLISPKR*VKNDFTSSKSSRILFLYSFSSNKLLS 597
C RT GETACT CC+D VS+FL S KR KN TS KSSRILFLYS S++ LLS
Sbjct: 1350 YC*RTMGETACTWCCKDSVSSFLSSIKRWFKNALTSYKSSRILFLYSVSNSWLLS 1186
>emb|BX831519.1|CNS0A0W1 Arabidopsis thaliana Full-length cDNA Complete sequence from clone
GSLTPGH12ZB08 of Hormone Treated Callus of strain col-0
of Arabidopsis thaliana (thale cress)
Length = 1792
Score = 293 bits (634), Expect = 4e-078
Identities = 128/176 (72%), Positives = 152/176 (86%)
Frame = -2 / +1
Query: 596 DRSLLLEKEYRNNILDDLLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQQHAPDSL 417
+RS LLE EYRN ILDDL EVK+FL QRL E+RN DT SLQH VQAVSP VLQQ++P+++
Sbjct: 1171 ERSQLLETEYRNKILDDLYEVKAFLNQRLIELRNEDTLSLQHHVQAVSPMVLQQYSPETI 1350
Query: 416 ENMLVEISSAISLLTNQKTLDLIMILNSKRFLDRLVSSLEEKKHHEVKLREGLGDLSVKR 237
E M+V+IS AISLLTN+K+ DLIMILNSKRFLDRLVS LEEKKH EVKLRE L D+ +R
Sbjct: 1351 EPMVVDISMAISLLTNKKSRDLIMILNSKRFLDRLVSELEEKKHREVKLRESLKDVGRRR 1530
Query: 236 MELQNALSSSWPKQEAAITKTRELKKLCESTLSSVFDGRPVYIIGEINTLLSSSVS 69
MELQN+LS+ WPKQEA ++KTRELK+LCE++LSS+FDGRPV I GEINTLL++ VS
Sbjct: 1531 MELQNSLSAIWPKQEAVLSKTRELKELCETSLSSMFDGRPVNIRGEINTLLNAGVS 1698
Score = 183 bits (395), Expect = 3e-045
Identities = 92/175 (52%), Positives = 108/175 (61%)
Frame = +1 / -3
Query: 73 TLELSKVLISPIIYTGLPSKTXXXXXXXXXXXXXXXXIAASCFGHDDDSAFCSSIRLTDK 252
T + VLISP+I+TGLPS ASCFGH +S FCSS+RL
Sbjct: 1694 TPAFNNVLISPLIFTGLPSNMEERLVSHSSLSSLVFESTASCFGHIAESEFCSSMRLLPT 1515
Query: 253 SPKPXXXXXXXXXXXXKLETNLSRNLLELRIIIRSRVF*LVSNEIADEISTSMFSKLSGA 432
S K ET+LSRNLLELRI+IRSR F VSNE+A E+ST++ S +SG
Sbjct: 1514 SFKLSLNFTSRCFFSSSSETSLSRNLLELRIMIRSRDFLFVSNEMAMEMSTTIGSMVSGE 1335
Query: 433 CCWRTKGETACT*CCRDDVSAFLISPKR*VKNDFTSSKSSRILFLYSFSSNKLLS 597
C RT GETACT CC+D VS+FL S KR KN TS KSSRILFLYS S++ LLS
Sbjct: 1334 YC*RTMGETACTWCCKDSVSSFLSSIKRWFKNALTSYKSSRILFLYSVSNSWLLS 1170
>emb|BX832407.1|CNS0A0ZV Arabidopsis thaliana Full-length cDNA Complete sequence from clone
GSLTPGH70ZF08 of Hormone Treated Callus of strain col-0
of Arabidopsis thaliana (thale cress)
Length = 1819
Score = 289 bits (626), Expect = 5e-077
Identities = 127/176 (72%), Positives = 151/176 (85%)
Frame = -2 / +2
Query: 596 DRSLLLEKEYRNNILDDLLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQQHAPDSL 417
+RS LLE EYRN LDDL EVK+FL QRL E+RN DT SLQH VQAVSP VLQQ++P+++
Sbjct: 1187 ERSQLLETEYRNKNLDDLYEVKAFLNQRLIELRNEDTLSLQHHVQAVSPMVLQQYSPETI 1366
Query: 416 ENMLVEISSAISLLTNQKTLDLIMILNSKRFLDRLVSSLEEKKHHEVKLREGLGDLSVKR 237
E M+V+IS AISLLTN+K+ DLIMILNSKRFLDRLVS LEEKKH EVKLRE L D+ +R
Sbjct: 1367 EPMVVDISMAISLLTNKKSRDLIMILNSKRFLDRLVSELEEKKHREVKLRESLKDVGRRR 1546
Query: 236 MELQNALSSSWPKQEAAITKTRELKKLCESTLSSVFDGRPVYIIGEINTLLSSSVS 69
MELQN+LS+ WPKQEA ++KTRELK+LCE++LSS+FDGRPV I GEINTLL++ VS
Sbjct: 1547 MELQNSLSAIWPKQEAELSKTRELKELCETSLSSMFDGRPVNIRGEINTLLNAGVS 1714
Score = 182 bits (392), Expect = 9e-045
Identities = 91/175 (52%), Positives = 108/175 (61%)
Frame = +1 / -2
Query: 73 TLELSKVLISPIIYTGLPSKTXXXXXXXXXXXXXXXXIAASCFGHDDDSAFCSSIRLTDK 252
T + VLISP+I+TGLPS +ASCFGH +S FCSS+RL
Sbjct: 1710 TPAFNNVLISPLIFTGLPSNMEDRLVSHSSLSSLVFESSASCFGHIAESEFCSSMRLLPT 1531
Query: 253 SPKPXXXXXXXXXXXXKLETNLSRNLLELRIIIRSRVF*LVSNEIADEISTSMFSKLSGA 432
S K ET+LSRNLLELRI+IRSR F VSNE+A E+ST++ S +SG
Sbjct: 1530 SFKLSLNFTSRCFFSSSSETSLSRNLLELRIMIRSRDFLFVSNEMAMEMSTTIGSMVSGE 1351
Query: 433 CCWRTKGETACT*CCRDDVSAFLISPKR*VKNDFTSSKSSRILFLYSFSSNKLLS 597
C RT GETACT CC+D VS+FL S KR KN TS KSSR LFLYS S++ LLS
Sbjct: 1350 YC*RTMGETACTWCCKDSVSSFLSSIKRWFKNALTSYKSSRFLFLYSVSNSWLLS 1186
>gb|CB259439.1|CB259439 50-E9537-013-002-C14-T7R MPIZ-ADIS-013 Arabidopsis thaliana cDNA
clone MPIZp770C142Q 5-PRIME, mRNA sequence
Length = 658
Score = 233 bits (503), Expect = 4e-060
Identities = 102/140 (72%), Positives = 120/140 (85%)
Frame = -2 / +1
Query: 596 DRSLLLEKEYRNNILDDLLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQQHAPDSL 417
+RS LLE EYRN ILDDL EVK+FL QRL E+RN DT SLQH VQAVSP VLQQ++P+++
Sbjct: 238 ERSQLLETEYRNKILDDLYEVKAFLNQRLIELRNEDTLSLQHHVQAVSPMVLQQYSPETI 417
Query: 416 ENMLVEISSAISLLTNQKTLDLIMILNSKRFLDRLVSSLEEKKHHEVKLREGLGDLSVKR 237
E M+V+IS AISLLTN+K+ DLIMILNSKRFLDRLVS LEEKKH EVKLRE L D+ +R
Sbjct: 418 EPMVVDISMAISLLTNKKSRDLIMILNSKRFLDRLVSELEEKKHREVKLRESLKDVGRRR 597
Query: 236 MELQNALSSSWPKQEAAITK 177
MELQN+LS+ WPKQEAA++K
Sbjct: 598 MELQNSLSAIWPKQEAALSK 657
Score = 164 bits (353), Expect = 2e-039
Identities = 81/137 (59%), Positives = 94/137 (68%)
Frame = +1 / -3
Query: 187 AASCFGHDDDSAFCSSIRLTDKSPKPXXXXXXXXXXXXKLETNLSRNLLELRIIIRSRVF 366
AASCFGH +S FCSS+RL S K ET+LSRNLLELRI+IRSR F
Sbjct: 647 AASCFGHIAESEFCSSMRLLPTSFKLSLNFTSRCFFSSSSETSLSRNLLELRIMIRSRDF 468
Query: 367 *LVSNEIADEISTSMFSKLSGACCWRTKGETACT*CCRDDVSAFLISPKR*VKNDFTSSK 546
VSNE+A E+ST++ S +SG C RT GETACT CC+D VS+FL S KR KN TS K
Sbjct: 467 LFVSNEMAMEMSTTIGSMVSGEYC*RTMGETACTWCCKDSVSSFLSSIKRWFKNALTSYK 288
Query: 547 SSRILFLYSFSSNKLLS 597
SSRILFLYS S++ LLS
Sbjct: 287 SSRILFLYSVSNSWLLS 237
>dbj|AP002032.1| Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MPH15
Length = 71277
Score = 116 bits (248), Expect(4) = 8e-053
Identities = 51/73 (69%), Positives = 63/73 (86%)
Frame = -2 / -1
Query: 545 LLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQQHAPDSLENMLVEISSAISLLTNQ 366
L +VK+FL QRL E+RN DT SLQH VQAVSP VLQQ++P+++E M+V+IS AISLLTN+
Sbjct: 67815 LCQVKAFLNQRLIELRNEDTLSLQHHVQAVSPMVLQQYSPETIEPMVVDISMAISLLTNK 67636
Query: 365 KTLDLIMILNSKR 327
K+ DLIMILNSKR
Sbjct: 67635 KSRDLIMILNSKR 67597
Score = 94.5 bits (200), Expect(4) = 2e-028
Identities = 46/78 (58%), Positives = 56/78 (71%)
Frame = +1 / +2
Query: 325 NLLELRIIIRSRVF*LVSNEIADEISTSMFSKLSGACCWRTKGETACT*CCRDDVSAFLI 504
+LLELRI+IRSR F VSNE+A E+ST++ S +SG C RT GETACT CC+D VS+FL
Sbjct: 67595 HLLELRIMIRSRDFLFVSNEMAMEMSTTIGSMVSGEYC*RTMGETACTWCCKDSVSSFLS 67774
Query: 505 SPKR*VKNDFTSSKSSRI 558
S KR KN T K + I
Sbjct: 67775 SIKRWFKNALT*HKRNDI 67828
Score = 87.7 bits (185), Expect(2) = 4e-021
Identities = 41/70 (58%), Positives = 47/70 (67%)
Frame = -2 / -1
Query: 404 VEISSAISLLTNQKTLDLIMILNSKRFLDRLVSSLEEKKHHEVKLREGLGDLSVKRMELQ 225
V I + +T T I I S RFLDRLVS LEEKKH EVKLRE L D+ +RMELQ
Sbjct: 67593 VRIKNLCQGITVSTTASDICICLSNRFLDRLVSELEEKKHREVKLRESLKDVGRRRMELQ 67414
Query: 224 NALSSSWPKQ 195
N+LS+ WPKQ
Sbjct: 67413 NSLSAIWPKQ 67384
Score = 76.7 bits (161), Expect(4) = 8e-053
Identities = 32/43 (74%), Positives = 40/43 (93%)
Frame = -2 / -1
Query: 197 QEAAITKTRELKKLCESTLSSVFDGRPVYIIGEINTLLSSSVS 69
QEAA++KTRELK+LCE++LSS+FDGRPV I GEINTLL++ VS
Sbjct: 67269 QEAALSKTRELKELCETSLSSMFDGRPVNIRGEINTLLNAGVS 67141
Score = 39.1 bits (79), Expect(4) = 8e-053
Identities = 18/26 (69%), Positives = 19/26 (73%)
Frame = -2 / -2
Query: 596 DRSLLLEKEYRNNILDDLLEVKSFLT 519
+RS LLE EYRN ILDDL EV LT
Sbjct: 67946 ERSQLLETEYRNKILDDLYEVLLSLT 67869
Score = 35.4 bits (71), Expect(2) = 4e-021
Identities = 16/45 (35%), Positives = 25/45 (55%)
Frame = -1 / -3
Query: 216 VIIMAKARGSNYKNKGTKEAV*VDTVVCF*WKTSVYNWRDQYFTE 82
+I++ ++NK T+ A+* ++ *WKT Y RDQY E
Sbjct: 67288 LILVLNTGSCTFENKRTQGAM*DKSLFHV*WKTCEYKRRDQYIVE 67154
Score = 34.1 bits (68), Expect(4) = 2e-028
Identities = 16/47 (34%), Positives = 21/47 (44%)
Frame = +1 / +2
Query: 73 TLELSKVLISPIIYTGLPSKTXXXXXXXXXXXXXXXXIAASCFGHDD 213
T + VLISP+I+TGLPS AASC + +
Sbjct: 67145 TPAFNNVLISPLIFTGLPSNMEERLVSHSSLSSLVFESAASCIQYQN 67285
Score = 33.6 bits (67), Expect(4) = 8e-053
Identities = 14/39 (35%), Positives = 25/39 (64%)
Frame = -3 / -2
Query: 310 FQA*KRRSITK*NYGKV*VIYLLSVWSCKTHCHHHGQSK 194
FQ+ +RRS K*+ + * +++ WSC+ H +GQ++
Sbjct: 67499 FQSWRRRSTVK*S*ERA*KMWVEDAWSCRIHFLLYGQNR 67383
Score = 30.9 bits (61), Expect(4) = 2e-028
Identities = 14/30 (46%), Positives = 15/30 (50%)
Frame = +2 / +2
Query: 533 LPRVNHPEYYSYILSPATNFYLQLSFETEL 622
+P NH YSYILSP L S EL
Sbjct: 67883 VPHTNHQGSYSYILSPTAGSSLLHSLRLEL 67972
Score = 23.1 bits (44), Expect(4) = 2e-028
Identities = 17/50 (34%), Positives = 24/50 (48%)
Frame = +3 / +1
Query: 201 WP***QCVLQLHTLNR*IT*TFP*FYFVMLLLF*A*N*SIQKPFRVKDHY 350
WP + +LQLH + I * +F +LLL *N I + R + Y
Sbjct: 67390 WPYSRK*ILQLHASSTHIFQALS*LHFTVLLLLQL*NKPI*ESVRQTNTY 67539
>gb|CB259351.1|CB259351 58-E9537-013-002-C16-T7R MPIZ-ADIS-013 Arabidopsis thaliana cDNA
clone MPIZp770C162Q 5-PRIME, mRNA sequence
Length = 579
Score = 191 bits (412), Expect = 2e-047
Identities = 85/113 (75%), Positives = 97/113 (85%)
Frame = -2 / +3
Query: 596 DRSLLLEKEYRNNILDDLLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQQHAPDSL 417
+RS LLE EYRN ILDDL EVK+FL QRL E+RN DT SLQH VQAVSP VLQQ++P+++
Sbjct: 237 ERSQLLETEYRNKILDDLYEVKAFLNQRLIELRNEDTLSLQHHVQAVSPMVLQQYSPETI 416
Query: 416 ENMLVEISSAISLLTNQKTLDLIMILNSKRFLDRLVSSLEEKKHHEVKLREGL 258
E M+V+IS AISLLTN+K+ DLIMILNSKRFLDRLVS LEEKKH EVKLRE L
Sbjct: 417 EPMVVDISMAISLLTNKKSRDLIMILNSKRFLDRLVSELEEKKHREVKLRESL 575
Score = 135 bits (290), Expect = 1e-030
Identities = 65/97 (67%), Positives = 76/97 (78%)
Frame = +1 / -3
Query: 307 ETNLSRNLLELRIIIRSRVF*LVSNEIADEISTSMFSKLSGACCWRTKGETACT*CCRDD 486
ET+LSRNLLELRI+IRSR F VSNE+A E+ST++ S +SG C RT GETACT CC+D
Sbjct: 526 ETSLSRNLLELRIMIRSRDFLFVSNEMAMEMSTTIGSMVSGEYC*RTMGETACTWCCKDS 347
Query: 487 VSAFLISPKR*VKNDFTSSKSSRILFLYSFSSNKLLS 597
VS+FL S KR KN TS KSSRILFLYS S++ LLS
Sbjct: 346 VSSFLSSIKRWFKNALTSYKSSRILFLYSVSNSWLLS 236
>dbj|BA000015.5| Arabidopsis thaliana DNA, chromosome 5, complete sequence
Length = 23810767
Score = 116 bits (248), Expect = 6e-025
Identities = 51/73 (69%), Positives = 63/73 (86%)
Frame = -2 / -2
Query: 545 LLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQQHAPDSLENMLVEISSAISLLTNQ 366
L +VK+FL QRL E+RN DT SLQH VQAVSP VLQQ++P+++E M+V+IS AISLLTN+
Sbjct: 1956600 LCQVKAFLNQRLIELRNEDTLSLQHHVQAVSPMVLQQYSPETIEPMVVDISMAISLLTNK 1956421
Query: 365 KTLDLIMILNSKR 327
K+ DLIMILNSKR
Sbjct: 1956420 KSRDLIMILNSKR 1956382
>ref|NC_003076.4| Arabidopsis thaliana chromosome 5, complete sequence
Length = 26992728
Score = 116 bits (248), Expect = 6e-025
Identities = 51/73 (69%), Positives = 63/73 (86%)
Frame = -2 / -3
Query: 545 LLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQQHAPDSLENMLVEISSAISLLTNQ 366
L +VK+FL QRL E+RN DT SLQH VQAVSP VLQQ++P+++E M+V+IS AISLLTN+
Sbjct: 2117563 LCQVKAFLNQRLIELRNEDTLSLQHHVQAVSPMVLQQYSPETIEPMVVDISMAISLLTNK 2117384
Query: 365 KTLDLIMILNSKR 327
K+ DLIMILNSKR
Sbjct: 2117383 KSRDLIMILNSKR 2117345
Database: At_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:09 PM
Number of letters in database: 908,940,872
Number of sequences in database: 1,013,581
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,194,595,540
Number of Sequences: 1013581
Number of extensions: 14620048
Number of successful extensions: 130412
Number of sequences better than 1.0e-020: 11
length of database: 302,980,290
effective HSP length: 55
effective length of database: 247,233,335
effective search space used: 37826700255
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)