BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3665525.2.1
(620 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF473884.1|BF473884 WHE0932_D09_H18ZS Wheat 5-15 DAP spi... 387 e-106
gb|CV066458.1|CV066458 WNEL4b5 Wheat EST endosperm library ... 371 e-101
gb|CA657314.1|CA657314 wlm0.pk0034.f11 wlm0 Triticum aestiv... 159 3e-037
gb|CA659140.1|CA659140 wlm1.pk0003.b6 wlm1 Triticum aestivu... 40 0.18
>gb|BF473884.1|BF473884 WHE0932_D09_H18ZS Wheat 5-15 DAP spike cDNA library Triticum
aestivum cDNA clone WHE0932_D09_H18, mRNA sequence
Length = 651
Score = 387 bits (195), Expect = e-106
Identities = 330/375 (88%)
Strand = Plus / Minus
Query: 246 gttggaccacctgtgcattcttttctttgatgaagctctagcatttttacctctgggtgg 305
||||||||||| ||||| ||||| | || |||||||||||||| |||||||| ||||||
Sbjct: 421 gttggaccacccgtgcactctttccgctggtgaagctctagcatctttacctccgggtgg 362
Query: 306 catagccctgagcattttggaatgtcccaaacagcaactgaagactccgtgcagtcaggt 365
||||||||||||||||||||||||||||| ||||||| ||| ||| | ||||| || |||
Sbjct: 361 catagccctgagcattttggaatgtcccagacagcaattgaggacgctgtgcactcgggt 302
Query: 366 tcacagagcgcatgtgtgttctcatggttgacgttcatagcaagcatccacgatccaatt 425
|| ||| || || |||||||| ||||||||||||||||| |||||||| ||||||||
Sbjct: 301 tcggtgagagcgtgcgtgttctcgtggttgacgttcatagcgagcatccaggatccaata 242
Query: 426 gtaacgtcctagatactaaacatacggaaactgttgttcctcaaagcaaccaagctagcc 485
||||| |||| | | | ||||| |||||||||||||| |||| |||||||||||| |||
Sbjct: 241 gtaacatcctcgttgttgaacatccggaaactgttgtttctcacagcaaccaagcttgcc 182
Query: 486 accacgtcagctgataaagcataaattggcccatatgcatgaagaaagtattctgatccg 545
||||| |||||||||||||||||||| ||||||||||||||||| ||||||||||||||
Sbjct: 181 accacatcagctgataaagcataaataggcccatatgcatgaaggaagtattctgatcca 122
Query: 546 agtaagaaggattggggctcataccatttcaacttaggatcagtaaaaacaggccccttc 605
|||| |||||| || ||||| ||||||||||| || ||||||||||||||||||||||||
Sbjct: 121 agtaggaaggactgtggctcgtaccatttcaatttgggatcagtaaaaacaggccccttc 62
Query: 606 ttcatgcatccaatg 620
|||||||||||||||
Sbjct: 61 ttcatgcatccaatg 47
>gb|CV066458.1|CV066458 WNEL4b5 Wheat EST endosperm library Triticum aestivum cDNA clone
WNEL4b5 5' similar to Oryza sativa, mRNA sequence
Length = 546
Score = 371 bits (187), Expect = e-101
Identities = 328/375 (87%)
Strand = Plus / Minus
Query: 246 gttggaccacctgtgcattcttttctttgatgaagctctagcatttttacctctgggtgg 305
||||||||||| ||||| ||||| | || |||||||| ||||| |||||||| ||||||
Sbjct: 378 gttggaccacccgtgcactctttccgctggtgaagctccagcatctttacctccgggtgg 319
Query: 306 catagccctgagcattttggaatgtcccaaacagcaactgaagactccgtgcagtcaggt 365
||||||||||||||||||||||| ||||| ||||||| ||| ||| | ||||| || |||
Sbjct: 318 catagccctgagcattttggaatatcccagacagcaattgaggacgctgtgcactcgggt 259
Query: 366 tcacagagcgcatgtgtgttctcatggttgacgttcatagcaagcatccacgatccaatt 425
|| || || ||||| |||||||| |||||||||||||||||||||||||| ||||||||
Sbjct: 258 tcgcacagagcatgcgtgttctcgtggttgacgttcatagcaagcatccaggatccaata 199
Query: 426 gtaacgtcctagatactaaacatacggaaactgttgttcctcaaagcaaccaagctagcc 485
||||| |||| | | ||||| || ||||||||||| |||| |||||||| ||| |||
Sbjct: 198 gtaacatcctcattgttgaacattcgaaaactgttgtttctcagagcaaccaggcttgcc 139
Query: 486 accacgtcagctgataaagcataaattggcccatatgcatgaagaaagtattctgatccg 545
||||| |||||||||||||||||||| ||||||||||||||||| ||||||||||||||
Sbjct: 138 accacatcagctgataaagcataaataggcccatatgcatgaaggaagtattctgatcca 79
Query: 546 agtaagaaggattggggctcataccatttcaacttaggatcagtaaaaacaggccccttc 605
|||| |||||| || ||||| ||||||||||| || ||||||||||||||||||||||||
Sbjct: 78 agtaggaaggactgtggctcgtaccatttcaatttgggatcagtaaaaacaggccccttc 19
Query: 606 ttcatgcatccaatg 620
|||||||||||||||
Sbjct: 18 ttcatgcatccaatg 4
>gb|CA657314.1|CA657314 wlm0.pk0034.f11 wlm0 Triticum aestivum cDNA clone wlm0.pk0034.f11
5' end, mRNA sequence
Length = 463
Score = 159 bits (80), Expect = 3e-037
Identities = 216/252 (85%), Gaps = 7/252 (2%)
Strand = Plus / Minus
Query: 375 gcatgtgtgttctcatggttgacgttcatagcaagcat-ccacgatccaattgtaacgtc 433
||||| |||||||| ||||||||||||||||||||||| ||| | ||||| ||||| ||
Sbjct: 388 gcatgcgtgttctcgtggttgacgttcatagcaagcatcccagaacccaatagtaacatc 329
Query: 434 ctagatactaaacatacggaaactgttgttcctcaa-agcaaccaagctagccacca-cg 491
|| | | ||||| ||||| ||||||| |||| |||||||| ||| ||||||| |
Sbjct: 328 ctcattgttgaacatt-ggaaaatgttgtttctcaggagcaaccaggcttgccaccaaca 270
Query: 492 tcagct-gataaagcataaattggcccatatgcatgaagaaagtattct-gatccga-gt 548
|||||| |||||||||||||| ||||||||||||||||| ||||||||| ||||| | ||
Sbjct: 269 tcagcttgataaagcataaataggcccatatgcatgaaggaagtattcttgatcccaagt 210
Query: 549 aagaaggattggggctcataccatttcaacttaggatcagtaaaaacaggccccttcttc 608
| |||||| || ||||| ||||||||||| || |||||||||||||||||||||||||||
Sbjct: 209 aggaaggactgtggctcgtaccatttcaatttgggatcagtaaaaacaggccccttcttc 150
Query: 609 atgcatccaatg 620
||||||||||||
Sbjct: 149 atgcatccaatg 138
>gb|CA659140.1|CA659140 wlm1.pk0003.b6 wlm1 Triticum aestivum cDNA clone wlm1.pk0003.b6 5'
end, mRNA sequence
Length = 538
Score = 40.1 bits (20), Expect = 0.18
Identities = 44/52 (84%)
Strand = Plus / Minus
Query: 246 gttggaccacctgtgcattcttttctttgatgaagctctagcatttttacct 297
||||||||||| ||||| ||||| | || |||||||| ||||| |||||||
Sbjct: 52 gttggaccacccgtgcactctttccgctggtgaagctccagcatctttacct 1
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 94,558
Number of Sequences: 636343
Number of extensions: 94558
Number of successful extensions: 23479
Number of sequences better than 0.5: 4
Number of HSP's better than 0.5 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 23468
Number of HSP's gapped (non-prelim): 9
length of query: 620
length of database: 367,240,239
effective HSP length: 19
effective length of query: 601
effective length of database: 355,149,722
effective search space: 213444982922
effective search space used: 213444982922
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)