BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QCM12h10.yg.2.1
         (339 letters)

Database: Sorghum_nucl_with_EST.fasta 
           832,831 sequences; 491,359,669 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CD428230.1|CD428230  ETH1_28_F10.g1_A002 Ethylene-treated...   327   4e-088
gb|BF657000.1|BF657000  OV2_19_G06.b1_A002 Ovary 2 (OV2) Sor...    44   0.008
gb|BG355549.1|BG355549  EM1_17_G07.b1_A002 Embryo 1 (EM1) So...    44   0.008
gb|BI643696.1|BI643696  EM1_19_F12.b1_A002 Embryo 1 (EM1) So...    44   0.008
gb|CF482724.1|CF482724  POL1_8_F04.g1_A002 Pollen Sorghum bi...    44   0.008
>gb|CD428230.1|CD428230 ETH1_28_F10.g1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA
           clone ETH1_28_F10_A002 5', mRNA sequence
          Length = 533

 Score =  327 bits (165), Expect = 4e-088
 Identities = 213/235 (90%)
 Strand = Plus / Minus

                                                                       
Query: 1   acactgttttcaaccaatcaaaaccagannnnntaaaagcaaatggagccataatccatg 60
           ||||||||||||||||| ||||||||||     |||||||||||||||||||||||||||
Sbjct: 243 acactgttttcaaccaaccaaaaccagatggattaaaagcaaatggagccataatccatg 184

                                                                       
Query: 61  ataccaccagaaaccagctagannnnnnnnnnnntatgtaaacaagtgtattcttggcaa 120
           ||||||||||||||||||||||            ||||||||||||||||||||||||||
Sbjct: 183 ataccaccagaaaccagctagatatattcatgattatgtaaacaagtgtattcttggcaa 124

                                                                       
Query: 121 taacactgtgtgcagcatacacagtcaatattattccaagctctattgcctttatgaagt 180
           |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct: 123 taacactgtgtgcagcatacacagtcaatattattccaagttctattgcctttatgaagt 64

                                                                  
Query: 181 ggcccctagcatacagcntgtaattttcagcaaaactcttgtgctgcacaacaaa 235
           ||| ||||||||||||| |||||||||||||||| ||||||||||||||||||||
Sbjct: 63  ggctcctagcatacagcctgtaattttcagcaaatctcttgtgctgcacaacaaa 9
>gb|BF657000.1|BF657000 OV2_19_G06.b1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA
           sequence
          Length = 455

 Score = 44.1 bits (22), Expect = 0.008
 Identities = 22/22 (100%)
 Strand = Plus / Minus

                                 
Query: 258 tttgcaccaccatgaaggattg 279
           ||||||||||||||||||||||
Sbjct: 357 tttgcaccaccatgaaggattg 336
>gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
           sequence
          Length = 502

 Score = 44.1 bits (22), Expect = 0.008
 Identities = 22/22 (100%)
 Strand = Plus / Minus

                                 
Query: 258 tttgcaccaccatgaaggattg 279
           ||||||||||||||||||||||
Sbjct: 108 tttgcaccaccatgaaggattg 87
>gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
           sequence
          Length = 631

 Score = 44.1 bits (22), Expect = 0.008
 Identities = 22/22 (100%)
 Strand = Plus / Minus

                                 
Query: 258 tttgcaccaccatgaaggattg 279
           ||||||||||||||||||||||
Sbjct: 108 tttgcaccaccatgaaggattg 87
>gb|CF482724.1|CF482724 POL1_8_F04.g1_A002 Pollen Sorghum bicolor cDNA clone
           POL1_8_F04_A002 5', mRNA sequence
          Length = 773

 Score = 44.1 bits (22), Expect = 0.008
 Identities = 22/22 (100%)
 Strand = Plus / Minus

                                 
Query: 258 tttgcaccaccatgaaggattg 279
           ||||||||||||||||||||||
Sbjct: 34  tttgcaccaccatgaaggattg 13
  Database: Sorghum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:54 PM
  Number of letters in database: 491,359,669
  Number of sequences in database:  832,831
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 62,721
Number of Sequences: 832831
Number of extensions: 62721
Number of successful extensions: 16149
Number of sequences better than  0.5: 5
Number of HSP's better than  0.5 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 16139
Number of HSP's gapped (non-prelim): 10
length of query: 339
length of database: 491,359,669
effective HSP length: 19
effective length of query: 320
effective length of database: 475,535,880
effective search space: 152171481600
effective search space used: 152171481600
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)