BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCF4h05.yg.2.1
(697 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG054341.1|BG054341 OV2_3_A09.b1_A002 Ovary 2 (OV2) Sorg... 385 e-105
gb|CF074169.1|CF074169 FE1_21_G07.g1_A002 Iron-deficient se... 115 6e-024
gb|CL170430.1|CL170430 104_371_10813804_116_31791_124 Sorgh... 68 1e-009
gb|CW243717.1|CW243717 104_704_11220201_148_37578_041 Sorgh... 60 3e-007
gb|BG559259.1|BG559259 RHIZ2_52_F03.b1_A003 Rhizome2 (RHIZ2... 42 0.067
>gb|BG054341.1|BG054341 OV2_3_A09.b1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA sequence
Length = 528
Score = 385 bits (194), Expect = e-105
Identities = 203/206 (98%)
Strand = Plus / Minus
Query: 66 tgaaaaagtcaaaaagttgtccgagtctatatatgtctcggctaagaacttgttcaccat 125
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 528 tgaaaaagtcaaaaagttgtccgagtctatatatgtctcggctaagaacttgttcaccat 469
Query: 126 ttccaccagcaacttttccttcaaatagtgcaatctgattaagtccaacatccctacctt 185
||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct: 468 ttccaccagcaacttttccttcaaagagtgcaatctgattaagtccaacatccctacctt 409
Query: 186 tgccaacctgaatatattcatgatgagttatgcatccttgacgcagagttgagttgaatc 245
|||||||||||||||| ||||||||||||||||||||||||||||| |||||||||||||
Sbjct: 408 tgccaacctgaatatactcatgatgagttatgcatccttgacgcagcgttgagttgaatc 349
Query: 246 ctgcatatatatcctcactgatattg 271
||||||||||||||||||||||||||
Sbjct: 348 ctgcatatatatcctcactgatattg 323
>gb|CF074169.1|CF074169 FE1_21_G07.g1_A002 Iron-deficient seedlings Sorghum bicolor cDNA
clone FE1_21_G07_A002 5', mRNA sequence
Length = 580
Score = 115 bits (58), Expect = 6e-024
Identities = 183/224 (81%), Gaps = 3/224 (1%)
Strand = Plus / Minus
Query: 248 gcatatatatcctcactgatattgatgctgcgagaggccttactaattccaccccttgtt 307
||||||||||| ||||| ||||| || || || ||||| ||||| ||||| | |||
Sbjct: 580 gcatatatatcttcacttatattaatcacacgggacgccttgctaatgccacctcgggtt 521
Query: 308 atatgaaaaattctatcaaaaacatctggatgaccatagtgcattctcactttcagcggg 367
|| ||||| || | |||||||||||| ||||| ||||||||||||| ||||| | ||
Sbjct: 520 atgtgaaatatccgatcaaaaacatcaggatgtccatagtgcattcgaactttga---gg 464
Query: 368 ttagcaagaacacgctgtcctaatgtcacaaaacttgtctcctgattagacataaatgag 427
| ||||||||||||||| || || || |||||||| |||||||| || |||||||||||
Sbjct: 463 taagcaagaacacgctgccccaaagtaacaaaactagtctcctgtttcgacataaatgac 404
Query: 428 gccagggaggaaacacttcccgtgaagacatgctccctaacacc 471
||||| || || ||||| |||||||| ||||||||||| |||||
Sbjct: 403 gccagcgaagagacactccccgtgaaaacatgctccctcacacc 360
>gb|CL170430.1|CL170430 104_371_10813804_116_31791_124 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10813804, DNA
sequence
Length = 746
Score = 67.9 bits (34), Expect = 1e-009
Identities = 76/90 (84%)
Strand = Plus / Minus
Query: 322 atcaaaaacatctggatgaccatagtgcattctcactttcagcgggttagcaagaacacg 381
||||||||||||||| || ||||| |||||||| ||||| || || | ||| ||||||||
Sbjct: 144 atcaaaaacatctgggtggccataatgcattctgactttaagtggatcagccagaacacg 85
Query: 382 ctgtcctaatgtcacaaaacttgtctcctg 411
||| || | || |||||||||||||||||
Sbjct: 84 ctgccccagagtgacaaaacttgtctcctg 55
Score = 42.1 bits (21), Expect = 0.067
Identities = 75/93 (80%)
Strand = Plus / Minus
Query: 115 ttgttcaccatttccaccagcaacttttccttcaaatagtgcaatctgattaagtccaac 174
|||||||||||| |||| |||||| || ||||||| | || | ||| || || || ||
Sbjct: 351 ttgttcaccattgccactagcaaccttggcttcaaacattgagacctggttgagccccac 292
Query: 175 atccctacctttgccaacctgaatatattcatg 207
||| |||||||| |||||||| ||||| |||||
Sbjct: 291 atctctacctttaccaacctggatatactcatg 259
>gb|CW243717.1|CW243717 104_704_11220201_148_37578_041 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11220201, DNA
sequence
Length = 587
Score = 60.0 bits (30), Expect = 3e-007
Identities = 54/62 (87%)
Strand = Plus / Minus
Query: 287 ttactaattccaccccttgttatatgaaaaattctatcaaaaacatctggatgaccatag 346
||||| ||||| || ||||||| |||||| || | |||||||| ||||||||||||||||
Sbjct: 572 ttacttattccgcctcttgttaaatgaaatatccgatcaaaaatatctggatgaccatag 513
Query: 347 tg 348
||
Sbjct: 512 tg 511
>gb|BG559259.1|BG559259 RHIZ2_52_F03.b1_A003 Rhizome2 (RHIZ2) Sorghum propinquum cDNA, mRNA
sequence
Length = 456
Score = 42.1 bits (21), Expect = 0.067
Identities = 30/33 (90%)
Strand = Plus / Minus
Query: 322 atcaaaaacatctggatgaccatagtgcattct 354
||||||||||||||| || ||||| ||||||||
Sbjct: 44 atcaaaaacatctgggtggccataatgcattct 12
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 144,445
Number of Sequences: 832831
Number of extensions: 144445
Number of successful extensions: 37781
Number of sequences better than 0.5: 5
Number of HSP's better than 0.5 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 37766
Number of HSP's gapped (non-prelim): 14
length of query: 697
length of database: 491,359,669
effective HSP length: 20
effective length of query: 677
effective length of database: 474,703,049
effective search space: 321373964173
effective search space used: 321373964173
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)