BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF13b04.yg.2.1
(370 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DV162815.1|DV162815 P3-J15 Sorghum bicolor greenbug-resp... 52 4e-005
gb|BF657000.1|BF657000 OV2_19_G06.b1_A002 Ovary 2 (OV2) Sor... 48 6e-004
gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) So... 48 6e-004
gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) So... 48 6e-004
gb|CF675663.1|CF675663 MtN3 Subtractive cDNA library from s... 46 0.002
gb|DR831467.1|DR831467 MM71 Sorghum bicolor greenbug-respon... 46 0.002
gb|DV162814.1|DV162814 P1-N10 Sorghum bicolor greenbug-resp... 46 0.002
gb|DV162819.1|DV162819 P5-B11 Sorghum bicolor greenbug-resp... 46 0.002
gb|CF675626.1|CF675626 THAU2 Subtractive cDNA library from ... 44 0.009
gb|DR831427.1|DR831427 MT54 Sorghum bicolor greenbug-respon... 44 0.009
gb|DR831428.1|DR831428 MM77 Sorghum bicolor greenbug-respon... 44 0.009
gb|DR831556.2|DR831556 MT75 Sorghum bicolor greenbug-respon... 44 0.009
gb|DV162795.1|DV162795 P1-M15 Sorghum bicolor greenbug-resp... 42 0.035
gb|DV162819.2|DV162819 P5-B11 Sorghum bicolor greenbug-resp... 42 0.035
gb|CW453485.1|CW453485 fsbb001f197i03f0 Sorghum methylation... 40 0.14
gb|CF675675.1|CF675675 RUBISCO Subtractive cDNA library fro... 40 0.14
gb|DR831561.1|DR831561 MT14 Sorghum bicolor greenbug-respon... 40 0.14
gb|DV162820.1|DV162820 P3-G6 Sorghum bicolor greenbug-respo... 40 0.14
>gb|DV162815.1|DV162815 P3-J15 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 229
Score = 52.0 bits (26), Expect = 4e-005
Identities = 26/26 (100%)
Strand = Plus / Minus
Query: 345 gtggtacctcggccgcgaccacgcta 370
||||||||||||||||||||||||||
Sbjct: 43 gtggtacctcggccgcgaccacgcta 18
>gb|BF657000.1|BF657000 OV2_19_G06.b1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA
sequence
Length = 455
Score = 48.1 bits (24), Expect = 6e-004
Identities = 102/128 (79%)
Strand = Plus / Plus
Query: 219 tttattacaatgcagttccaaatgtgctctgtattttttactttctcacttggaacaaga 278
||||||||||||||| |||| | || || || ||||| || || || |||||||||||
Sbjct: 257 tttattacaatgcagctccagttctgttcggtgtttttcaccttttcgcttggaacaagg 316
Query: 279 acccactactttggccgtgcaatattacatggaggtgcaaagtatagagcaaccggtaga 338
|| || |||||||| | |||| | ||||| ||||||||||||| ||| || || |||
Sbjct: 317 acacattactttggtagaacaatccttcatggtggtgcaaagtataaagctactggaaga 376
Query: 339 ggttttgt 346
||||||||
Sbjct: 377 ggttttgt 384
>gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 502
Score = 48.1 bits (24), Expect = 6e-004
Identities = 102/128 (79%)
Strand = Plus / Plus
Query: 219 tttattacaatgcagttccaaatgtgctctgtattttttactttctcacttggaacaaga 278
||||||||||||||| |||| | || || || ||||| || || || |||||||||||
Sbjct: 8 tttattacaatgcagctccagttctgttcggtgtttttcaccttttcgcttggaacaagg 67
Query: 279 acccactactttggccgtgcaatattacatggaggtgcaaagtatagagcaaccggtaga 338
|| || |||||||| | |||| | ||||| ||||||||||||| ||| || || |||
Sbjct: 68 acacattactttggtagaacaatccttcatggtggtgcaaagtataaagctactggaaga 127
Query: 339 ggttttgt 346
||||||||
Sbjct: 128 ggttttgt 135
>gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 631
Score = 48.1 bits (24), Expect = 6e-004
Identities = 102/128 (79%)
Strand = Plus / Plus
Query: 219 tttattacaatgcagttccaaatgtgctctgtattttttactttctcacttggaacaaga 278
||||||||||||||| |||| | || || || ||||| || || || |||||||||||
Sbjct: 8 tttattacaatgcagctccagttctgttcggtgtttttcaccttttcgcttggaacaagg 67
Query: 279 acccactactttggccgtgcaatattacatggaggtgcaaagtatagagcaaccggtaga 338
|| || |||||||| | |||| | ||||| ||||||||||||| ||| || || |||
Sbjct: 68 acacattactttggtagaacaatccttcatggtggtgcaaagtataaagctactggaaga 127
Query: 339 ggttttgt 346
||||||||
Sbjct: 128 ggttttgt 135
>gb|CF675663.1|CF675663 MtN3 Subtractive cDNA library from sorghum infested by greenbug
aphids Sorghum bicolor cDNA clone MtN3 similar to
MtN3-like protein, mRNA sequence
Length = 194
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Plus
Query: 348 gtacctcggccgcgaccacgcta 370
|||||||||||||||||||||||
Sbjct: 143 gtacctcggccgcgaccacgcta 165
>gb|DR831467.1|DR831467 MM71 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 751
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 348 gtacctcggccgcgaccacgcta 370
|||||||||||||||||||||||
Sbjct: 127 gtacctcggccgcgaccacgcta 105
>gb|DV162814.1|DV162814 P1-N10 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 198
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Plus
Query: 348 gtacctcggccgcgaccacgcta 370
|||||||||||||||||||||||
Sbjct: 95 gtacctcggccgcgaccacgcta 117
>gb|DV162819.1|DV162819 P5-B11 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 234
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtacata 23
|||||||||||||||||||||||
Sbjct: 84 gcggccgcccgggcaggtacata 62
>gb|CF675626.1|CF675626 THAU2 Subtractive cDNA library from sorghum infested by greenbug
aphids Sorghum bicolor cDNA clone THAU2 similar to
Thaumatin-like protein, mRNA sequence
Length = 747
Score = 44.1 bits (22), Expect = 0.009
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtacat 22
||||||||||||||||||||||
Sbjct: 204 gcggccgcccgggcaggtacat 225
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 211 gcggccgcccgggcaggtac 192
>gb|DR831427.1|DR831427 MT54 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 199
Score = 44.1 bits (22), Expect = 0.009
Identities = 22/22 (100%)
Strand = Plus / Minus
Query: 348 gtacctcggccgcgaccacgct 369
||||||||||||||||||||||
Sbjct: 22 gtacctcggccgcgaccacgct 1
>gb|DR831428.1|DR831428 MM77 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 299
Score = 44.1 bits (22), Expect = 0.009
Identities = 22/22 (100%)
Strand = Plus / Minus
Query: 346 tggtacctcggccgcgaccacg 367
||||||||||||||||||||||
Sbjct: 24 tggtacctcggccgcgaccacg 3
>gb|DR831556.2|DR831556 MT75 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 309
Score = 44.1 bits (22), Expect = 0.009
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 348 gtacctcggccgcgaccacgct 369
||||||||||||||||||||||
Sbjct: 205 gtacctcggccgcgaccacgct 226
>gb|DV162795.1|DV162795 P1-M15 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 282
Score = 42.1 bits (21), Expect = 0.035
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtaca 21
|||||||||||||||||||||
Sbjct: 32 gcggccgcccgggcaggtaca 52
>gb|DV162819.2|DV162819 P5-B11 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 172
Score = 42.1 bits (21), Expect = 0.035
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtaca 21
|||||||||||||||||||||
Sbjct: 21 gcggccgcccgggcaggtaca 1
>gb|CW453485.1|CW453485 fsbb001f197i03f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f197i03, DNA
sequence
Length = 512
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 51 ttatctggtgttggagaatc 70
||||||||||||||||||||
Sbjct: 313 ttatctggtgttggagaatc 332
>gb|CF675675.1|CF675675 RUBISCO Subtractive cDNA library from sorghum infested by greenbug
aphids Sorghum bicolor cDNA clone RUBISCO similar to
Ribulose 1,5-biphosphate carboxylase, mRNA sequence
Length = 638
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 225 gcggccgcccgggcaggtac 244
>gb|DR831561.1|DR831561 MT14 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 112
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 2 gcggccgcccgggcaggtac 21
>gb|DV162820.1|DV162820 P3-G6 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 223
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 119 gcggccgcccgggcaggtac 100
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 97,376
Number of Sequences: 832831
Number of extensions: 97376
Number of successful extensions: 26701
Number of sequences better than 0.5: 18
Number of HSP's better than 0.5 without gapping: 18
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 26673
Number of HSP's gapped (non-prelim): 22
length of query: 370
length of database: 491,359,669
effective HSP length: 19
effective length of query: 351
effective length of database: 475,535,880
effective search space: 166913093880
effective search space used: 166913093880
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)