BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAE40a10.yg.2.1
(709 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) So... 936 0.0
gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) So... 868 0.0
gb|CF482724.1|CF482724 POL1_8_F04.g1_A002 Pollen Sorghum bi... 759 0.0
gb|BF657000.1|BF657000 OV2_19_G06.b1_A002 Ovary 2 (OV2) Sor... 484 e-135
gb|BZ338573.1|BZ338573 ia96c08.g1 WGS-SbicolorF (JM107 adap... 174 7e-042
gb|CL170431.1|CL170431 104_371_10813804_148_31790_124 Sorgh... 64 2e-008
gb|CD219775.1|CD219775 CCC1_58_A07.g1_A007 Callus culture/c... 64 2e-008
gb|CW256112.1|CW256112 104_721_11226607_148_35144_032 Sorgh... 62 7e-008
gb|DV162795.1|DV162795 P1-M15 Sorghum bicolor greenbug-resp... 44 0.017
gb|CW476266.1|CW476266 fsbb001f233a20k0 Sorghum methylation... 42 0.068
gb|CF675626.1|CF675626 THAU2 Subtractive cDNA library from ... 42 0.068
gb|DV162819.1|DV162819 P5-B11 Sorghum bicolor greenbug-resp... 42 0.068
gb|DV162819.2|DV162819 P5-B11 Sorghum bicolor greenbug-resp... 42 0.068
gb|CF675675.1|CF675675 RUBISCO Subtractive cDNA library fro... 40 0.27
gb|DR831561.1|DR831561 MT14 Sorghum bicolor greenbug-respon... 40 0.27
gb|DV162820.1|DV162820 P3-G6 Sorghum bicolor greenbug-respo... 40 0.27
>gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 631
Score = 936 bits (472), Expect = 0.0
Identities = 526/544 (96%)
Strand = Plus / Minus
Query: 17 gtacacaatgccatactgaaacatgagaaatctaaggctgaggattgtctcaagaatacg 76
||||||||||||||||||||| ||||||||||||||||| ||||||||||||||||||||
Sbjct: 544 gtacacaatgccatactgaaatatgagaaatctaaggctcaggattgtctcaagaatacg 485
Query: 77 tcctctgaatgtctggatatgcgcctgctcctcatcccaccaagattcccaactgttgtc 136
|||||||||||||||||||||| |||||||||||||||||||||||||||||||| | ||
Sbjct: 484 tcctctgaatgtctggatatgctcctgctcctcatcccaccaagattcccaactgctatc 425
Query: 137 accctttacaccaactccacctttataaagtaaccagtttgtccagtcatcaaagtcctc 196
|||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct: 424 gccctttacaccaactccacctttataaagtaaccagtttgtccagtcatcgaagtcctc 365
Query: 197 aacagtcttttgccactcgaaaccagaagggttgaaaatgtaaggcgcgaaaagccagga 256
|||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
Sbjct: 364 aacagtcttttgccactcgaaaccagaagggttgaatatgtaaggcgcgaaaagccagga 305
Query: 257 cataacgaggaaccaactactgatagtaatcagaataaatgaagatgaaccgccttttgt 316
||||||||||||||||||||||||||||||||| |||||||||||||||||||| || ||
Sbjct: 304 cataacgaggaaccaactactgatagtaatcagtataaatgaagatgaaccgcccttcgt 245
Query: 317 gtatccatatgcaatgtaaacgatcaggaggagagcaacctcaagcgctttgacaaagtg 376
||||||||||| |||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct: 244 gtatccatatgaaatgtaaacgatgaggaggagagcaacctcaagcgctttgacaaagtg 185
Query: 377 acttcgagaatagagcctataattttcagcaaactttatgtgccgaacaacaaaacctct 436
||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct: 184 acttcgagaatagagcctataattttcagcaaattttatgtgccgaacaacaaaacctct 125
Query: 437 tccagtagcacgatactttgcaccgccatgaaggattgttctaccaaagtaatgtgtcct 496
||||||||| |||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct: 124 tccagtagctttatactttgcaccaccatgaaggattgttctaccaaagtaatgtgtcct 65
Query: 497 tgttccaagcgaaaaggtgaaaaacaccgaacagaactggagctgcattgtaataaaact 556
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 64 tgttccaagcgaaaaggtgaaaaacaccgaacagaactggagctgcattgtaataaaact 5
Query: 557 aaaa 560
||||
Sbjct: 4 aaaa 1
>gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 502
Score = 868 bits (438), Expect = 0.0
Identities = 486/502 (96%)
Strand = Plus / Minus
Query: 59 gattgtctcaagaatacgtcctctgaatgtctggatatgcgcctgctcctcatcccacca 118
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct: 502 gattgtctcaagaatacgtcctctgaatgtctggatatgctcctgctcctcatcccacca 443
Query: 119 agattcccaactgttgtcaccctttacaccaactccacctttataaagtaaccagtttgt 178
||||||||||||| | || |||||||||||||||||||||||||||||||||||||||||
Sbjct: 442 agattcccaactgctatcgccctttacaccaactccacctttataaagtaaccagtttgt 383
Query: 179 ccagtcatcaaagtcctcaacagtcttttgccactcgaaaccagaagggttgaaaatgta 238
||||||||| |||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct: 382 ccagtcatcgaagtcctcaacagtcttttgccactcgaaaccagaagggttgaatatgta 323
Query: 239 aggcgcgaaaagccaggacataacgaggaaccaactactgatagtaatcagaataaatga 298
||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct: 322 aggcgcgaaaagccaggacataacgaggaaccaactactgatagtaatcagtataaatga 263
Query: 299 agatgaaccgccttttgtgtatccatatgcaatgtaaacgatcaggaggagagcaacctc 358
|||||||||||| || ||||||||||||| |||||||||||| |||||||||||||||||
Sbjct: 262 agatgaaccgcccttcgtgtatccatatgaaatgtaaacgatgaggaggagagcaacctc 203
Query: 359 aagcgctttgacaaagtgacttcgagaatagagcctataattttcagcaaactttatgtg 418
||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct: 202 aagcgctttgacaaagtgacttcgagaatagagcctataattttcagcaaattttatgtg 143
Query: 419 ccgaacaacaaaacctcttccagtagcacgatactttgcaccgccatgaaggattgttct 478
||||||||||||||||||||||||||| |||||||||||| |||||||||||||||||
Sbjct: 142 ccgaacaacaaaacctcttccagtagctttatactttgcaccaccatgaaggattgttct 83
Query: 479 accaaagtaatgtgtccttgttccaagcgaaaaggtgaaaaacaccgaacagaactggag 538
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 82 accaaagtaatgtgtccttgttccaagcgaaaaggtgaaaaacaccgaacagaactggag 23
Query: 539 ctgcattgtaataaaactaaaa 560
||||||||||||||||||||||
Sbjct: 22 ctgcattgtaataaaactaaaa 1
>gb|CF482724.1|CF482724 POL1_8_F04.g1_A002 Pollen Sorghum bicolor cDNA clone
POL1_8_F04_A002 5', mRNA sequence
Length = 773
Score = 759 bits (383), Expect = 0.0
Identities = 451/471 (95%), Gaps = 2/471 (0%)
Strand = Plus / Minus
Query: 17 gtacacaatgccatactgaaacatgagaaatctaaggctgaggattgtctcaagaatacg 76
||||||||||||||||||||| ||||||||||||||||| ||||||||||||||||||||
Sbjct: 469 gtacacaatgccatactgaaatatgagaaatctaaggctcaggattgtctcaagaatacg 410
Query: 77 tcctctgaatgtctggatatgcgcctgctcctcatcccaccaagattcccaactgttgtc 136
|||||||||||||||||||||| |||||||||||||||||||||||||||||||| | ||
Sbjct: 409 tcctctgaatgtctggatatgctcctgctcctcatcccaccaagattcccaactgctatc 350
Query: 137 accctttacaccaactccacctttataaagtaaccagtttgtccagtcatcaaagtcctc 196
|||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct: 349 gccctttacaccaactccacctttataaagtaaccagtttgtccagtcatcgaagtcctc 290
Query: 197 aacagtcttttgccactcgaaaccagaagggttgaaaatgtaaggcgcgaaaagccagga 256
|||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
Sbjct: 289 aacagtcttttgccactcgaaaccagaagggttgaatatgtaaggcgcgaaaagccagga 230
Query: 257 cataacgaggaaccaactactgatagtaatcagaataaatgaagatgaaccgccttttgt 316
||||||||||||||||||||||||||||||||| |||||||||||||||||||| || ||
Sbjct: 229 cataacgaggaaccaactactgatagtaatcagtataaatgaagatgaaccgcccttcgt 170
Query: 317 gtatccatatgcaatgtaaacgatcaggaggagagcaacctcaagcgctttgacaaagtg 376
||||||||||| |||||||||||| |||||||||||||||||||| ||||||||||||||
Sbjct: 169 gtatccatatgaaatgtaaacgatgaggaggagagcaacctcaagtgctttgacaaagtg 110
Query: 377 acttcgagaatagagcctataattttcagcaaactttatgtgccgaacaacaaaacctct 436
||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct: 109 acttcgagaatagagcctataattttcagcaaattttatgtgccgaacaacaaaacctct 50
Query: 437 tccagtagcacgatactttgcaccgccatgaaggattgttctaccaaagta 487
||||||||| |||||||||||| ||||||||||||| ||||||||||||
Sbjct: 49 tccagtagc-ttatactttgcaccaccatgaaggattg-tctaccaaagta 1
>gb|BF657000.1|BF657000 OV2_19_G06.b1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA
sequence
Length = 455
Score = 484 bits (244), Expect = e-135
Identities = 322/355 (90%)
Strand = Plus / Minus
Query: 355 cctcaagcgctttgacaaagtgacttcgagaatagagcctataattttcagcaaacttta 414
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
Sbjct: 455 cctcaagcgctttgacaaagtgacttcgagaatagagcctataattttcagcaaatttta 396
Query: 415 tgtgccgaacaacaaaacctcttccagtagcacgatactttgcaccgccatgaaggattg 474
||||||||||||||||||||||||||||||| |||||||||||| |||||||||||||
Sbjct: 395 tgtgccgaacaacaaaacctcttccagtagctttatactttgcaccaccatgaaggattg 336
Query: 475 ttctaccaaagtaatgtgtccttgttccaagcgaaaaggtgaaaaacaccgaacagaact 534
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 335 ttctaccaaagtaatgtgtccttgttccaagcgaaaaggtgaaaaacaccgaacagaact 276
Query: 535 ggagctgcattgtaataaaactaaaaacagccttcatcagccccagctnnagaatgaaac 594
||||||||||||||||||||||||||| ||||||||||||||| |||| | ||||||||
Sbjct: 275 ggagctgcattgtaataaaactaaaaatagccttcatcagccctagctccaaaatgaaac 216
Query: 595 ccattatcatcgggactgnngtnaaaataccaatctnnnncaaaaattgagcattcaaag 654
||||||||||||| |||| || ||||||||||||| ||| ||||||||||||||||
Sbjct: 215 ccattatcatcggtactgctgtaaaaataccaatctggaccaagaattgagcattcaaag 156
Query: 655 ctgcatctagtncannnnnnnccaaaaacnnngctnggcgagatattgagtagtc 709
| |||||||| || |||||||| ||| |||||||||||||||||||
Sbjct: 155 cagcatctagcgcagtgttccccaaaaacctagcttggcgagatattgagtagtc 101
>gb|BZ338573.1|BZ338573 ia96c08.g1 WGS-SbicolorF (JM107 adapted methyl filtered) Sorghum
bicolor genomic clone ia96c08 5', DNA sequence
Length = 649
Score = 174 bits (88), Expect = 7e-042
Identities = 100/104 (96%)
Strand = Plus / Minus
Query: 100 cctgctcctcatcccaccaagattcccaactgttgtcaccctttacaccaactccacctt 159
|||||||||||||||||||||||||||||||| | || ||||||||||||||||||||||
Sbjct: 328 cctgctcctcatcccaccaagattcccaactgctatcgccctttacaccaactccacctt 269
Query: 160 tataaagtaaccagtttgtccagtcatcaaagtcctcaacagtc 203
|||||||||||||||||||||||||||| |||||||||||||||
Sbjct: 268 tataaagtaaccagtttgtccagtcatcgaagtcctcaacagtc 225
Score = 149 bits (75), Expect = 4e-034
Identities = 84/87 (96%)
Strand = Plus / Minus
Query: 17 gtacacaatgccatactgaaacatgagaaatctaaggctgaggattgtctcaagaatacg 76
||||||||||||||||||||| ||||||||||||||||| ||||||||||||||||||||
Sbjct: 522 gtacacaatgccatactgaaatatgagaaatctaaggctcaggattgtctcaagaatacg 463
Query: 77 tcctctgaatgtctggatatgcgcctg 103
|||||||||||||||||||||| ||||
Sbjct: 462 tcctctgaatgtctggatatgctcctg 436
>gb|CL170431.1|CL170431 104_371_10813804_148_31790_124 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10813804, DNA
sequence
Length = 656
Score = 63.9 bits (32), Expect = 2e-008
Identities = 77/92 (83%)
Strand = Plus / Plus
Query: 381 cgagaatagagcctataattttcagcaaactttatgtgccgaacaacaaaacctcttcca 440
|||| |||||||||||| |||||||||||||| ||||| |||||||| ||||| |||
Sbjct: 108 cgagcatagagcctatagttttcagcaaacttcttgtgctctacaacaaagcctctgcca 167
Query: 441 gtagcacgatactttgcaccgccatgaaggat 472
|| || |||||||| || || || ||||||||
Sbjct: 168 gtggcccgatacttcgctcctccgtgaaggat 199
>gb|CD219775.1|CD219775 CCC1_58_A07.g1_A007 Callus culture/cell suspension Sorghum bicolor
cDNA clone CCC1_58_A07_A007 5', mRNA sequence
Length = 669
Score = 63.9 bits (32), Expect = 2e-008
Identities = 77/92 (83%)
Strand = Plus / Minus
Query: 381 cgagaatagagcctataattttcagcaaactttatgtgccgaacaacaaaacctcttcca 440
|||| |||||||||||| |||||||||||||| ||||| |||||||| ||||| |||
Sbjct: 451 cgagcatagagcctatagttttcagcaaacttcttgtgctctacaacaaagcctctgcca 392
Query: 441 gtagcacgatactttgcaccgccatgaaggat 472
|| || |||||||| || || || ||||||||
Sbjct: 391 gtggcccgatacttcgctcctccgtgaaggat 360
>gb|CW256112.1|CW256112 104_721_11226607_148_35144_032 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11226607, DNA
sequence
Length = 678
Score = 61.9 bits (31), Expect = 7e-008
Identities = 115/143 (80%)
Strand = Plus / Minus
Query: 363 gctttgacaaagtgacttcgagaatagagcctataattttcagcaaactttatgtgccga 422
||||| ||||||||||| || || || | || |||||| ||||| ||||| |||||
Sbjct: 275 gctttcacaaagtgactccgggagtacatccgataattctcagcgaacttcgcatgccgc 216
Query: 423 acaacaaaacctcttccagtagcacgatactttgcaccgccatgaaggattgttctacca 482
|||||||| ||||| |||||||| | ||||||||| || || ||||| ||||| || |||
Sbjct: 215 acaacaaaccctctcccagtagctctatactttgctccaccgtgaagtattgtcctgcca 156
Query: 483 aagtaatgtgtccttgttccaag 505
|||| || ||| ||||||||||
Sbjct: 155 tagtagtgagtctttgttccaag 133
>gb|DV162795.1|DV162795 P1-M15 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 282
Score = 44.1 bits (22), Expect = 0.017
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtacac 22
||||||||||||||||||||||
Sbjct: 32 gcggccgcccgggcaggtacac 53
>gb|CW476266.1|CW476266 fsbb001f233a20k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f233a20, DNA
sequence
Length = 554
Score = 42.1 bits (21), Expect = 0.068
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 507 gaaaaggtgaaaaacaccgaa 527
|||||||||||||||||||||
Sbjct: 159 gaaaaggtgaaaaacaccgaa 139
>gb|CF675626.1|CF675626 THAU2 Subtractive cDNA library from sorghum infested by greenbug
aphids Sorghum bicolor cDNA clone THAU2 similar to
Thaumatin-like protein, mRNA sequence
Length = 747
Score = 42.1 bits (21), Expect = 0.068
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtaca 21
|||||||||||||||||||||
Sbjct: 204 gcggccgcccgggcaggtaca 224
Score = 40.1 bits (20), Expect = 0.27
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 211 gcggccgcccgggcaggtac 192
>gb|DV162819.1|DV162819 P5-B11 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 234
Score = 42.1 bits (21), Expect = 0.068
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtaca 21
|||||||||||||||||||||
Sbjct: 84 gcggccgcccgggcaggtaca 64
>gb|DV162819.2|DV162819 P5-B11 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 172
Score = 42.1 bits (21), Expect = 0.068
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtaca 21
|||||||||||||||||||||
Sbjct: 21 gcggccgcccgggcaggtaca 1
>gb|CF675675.1|CF675675 RUBISCO Subtractive cDNA library from sorghum infested by greenbug
aphids Sorghum bicolor cDNA clone RUBISCO similar to
Ribulose 1,5-biphosphate carboxylase, mRNA sequence
Length = 638
Score = 40.1 bits (20), Expect = 0.27
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 225 gcggccgcccgggcaggtac 244
>gb|DR831561.1|DR831561 MT14 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 112
Score = 40.1 bits (20), Expect = 0.27
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 2 gcggccgcccgggcaggtac 21
>gb|DV162820.1|DV162820 P3-G6 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 223
Score = 40.1 bits (20), Expect = 0.27
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 119 gcggccgcccgggcaggtac 100
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 156,147
Number of Sequences: 832831
Number of extensions: 156147
Number of successful extensions: 41355
Number of sequences better than 0.5: 16
Number of HSP's better than 0.5 without gapping: 15
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 41332
Number of HSP's gapped (non-prelim): 23
length of query: 709
length of database: 491,359,669
effective HSP length: 20
effective length of query: 689
effective length of database: 474,703,049
effective search space: 327070400761
effective search space used: 327070400761
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)