BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2591368.2.1
(543 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW185910.1|CW185910 104_603_11166295_148_36698_020 Sorgh... 208 4e-052
gb|CD425442.1|CD425442 SA1_12_C07.b1_A002 Salicylic acid-tr... 208 4e-052
gb|CW110059.1|CW110059 104_482_11103476_148_34514_070 Sorgh... 184 6e-045
gb|CD209097.1|CD209097 HS1_46_G10.b1_A012 Heat-shocked seed... 131 7e-029
gb|BZ780700.1|BZ780700 ii19b06.g1 WGS-SbicolorF (DH5a methy... 60 2e-007
gb|CL159238.1|CL159238 104_348_10804110_116_31380_318 Sorgh... 56 3e-006
gb|CD425602.1|CD425602 SA1_13_B06.b1_A002 Salicylic acid-tr... 56 3e-006
gb|CD425910.1|CD425910 SA1_15_B06.b1_A002 Salicylic acid-tr... 54 1e-005
gb|CW371298.1|CW371298 fsbb001f046l04f0 Sorghum methylation... 52 5e-005
gb|CX613321.1|CX613321 GABR1_7_B08.g1_A002 GA- or brassinol... 40 0.21
>gb|CW185910.1|CW185910 104_603_11166295_148_36698_020 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11166295, DNA
sequence
Length = 613
Score = 208 bits (105), Expect = 4e-052
Identities = 132/141 (93%)
Strand = Plus / Plus
Query: 401 tgctggtgctggcgcacgaagcggcggatccggtcgagcgcgacctccatggtgtcgtcg 460
||||||||||||||||||||| || |||||| | |||||| ||||||||||||||||||
Sbjct: 164 tgctggtgctggcgcacgaagtggtggatcctgcggagcgccacctccatggtgtcgtcg 223
Query: 461 tccatgttggcgtagcagacgcggaaccagccgggctcgttgcagtggaacgacgtgccg 520
||||||||||||| ||||||||||||||| |||||||||||||||||||||||||||||
Sbjct: 224 tccatgttggcgtggcagacgcggaaccatccgggctcgttgcagtggaacgacgtgcct 283
Query: 521 ggcgacacgttgagcttcacc 541
|||||||||||||||||||||
Sbjct: 284 ggcgacacgttgagcttcacc 304
Score = 174 bits (88), Expect = 5e-042
Identities = 143/161 (88%), Gaps = 9/161 (5%)
Strand = Plus / Plus
Query: 254 tagctggcgtggaccatcggggactgcggcgac---agcaacgccaaggggctggggatg 310
||||||||||||||||| || |||||||||||| ||||||||||||||||||| ||||
Sbjct: 5 tagctggcgtggaccatgggcgactgcggcgacgacagcaacgccaaggggctggagatg 64
Query: 311 gcgaggtgcgaagcggtggttgctccccggcgcggcaagctgaggcggaggggccgcgtg 370
|||||||||||||| | ||||||||||||||||||||||||| ||| | ||||||
Sbjct: 65 gcgaggtgcgaagcag------ctccccggcgcggcaagctgaggcgcaggcggcgcgtg 118
Query: 371 gccgcccagcgctcggccttggccttgctgtgctggtgctg 411
||||||||||||||||||||||||||||| ||||| |||||
Sbjct: 119 gccgcccagcgctcggccttggccttgctctgctgctgctg 159
>gb|CD425442.1|CD425442 SA1_12_C07.b1_A002 Salicylic acid-treated seedlings Sorghum bicolor
cDNA clone SA1_12_C07_A002 3', mRNA sequence
Length = 613
Score = 208 bits (105), Expect = 4e-052
Identities = 132/141 (93%)
Strand = Plus / Minus
Query: 401 tgctggtgctggcgcacgaagcggcggatccggtcgagcgcgacctccatggtgtcgtcg 460
||||||||||||||||||||| || |||||| | |||||| ||||||||||||||||||
Sbjct: 216 tgctggtgctggcgcacgaagtggtggatcctgcggagcgccacctccatggtgtcgtcg 157
Query: 461 tccatgttggcgtagcagacgcggaaccagccgggctcgttgcagtggaacgacgtgccg 520
||||||||||||| ||||||||||||||| |||||||||||||||||||||||||||||
Sbjct: 156 tccatgttggcgtggcagacgcggaaccatccgggctcgttgcagtggaacgacgtgcct 97
Query: 521 ggcgacacgttgagcttcacc 541
|||||||||||||||||||||
Sbjct: 96 ggcgacacgttgagcttcacc 76
Score = 200 bits (101), Expect = 9e-050
Identities = 176/200 (88%), Gaps = 12/200 (6%)
Strand = Plus / Minus
Query: 215 caccgtacagccagtcttaccgaacgctcggtgactagctagctggcgtggaccatcggg 274
|||| ||||||||||||| ||||| ||||||||||| ||||||||||||||||| ||
Sbjct: 411 caccctacagccagtcttgccgaatgctcggtgact---tagctggcgtggaccatgggc 355
Query: 275 gactgcggcgac---agcaacgccaaggggctggggatggcgaggtgcgaagcggtggtt 331
|||||||||||| ||||||||||||||||||| ||||||||||||||||||
Sbjct: 354 gactgcggcgacgacagcaacgccaaggggctggagatggcgaggtgcgaagc------a 301
Query: 332 gctccccggcgcggcaagctgaggcggaggggccgcgtggccgcccagcgctcggccttg 391
|||||||||||||||||||||||||| ||| | |||||||||||||||||||||||||||
Sbjct: 300 gctccccggcgcggcaagctgaggcgcaggcggcgcgtggccgcccagcgctcggccttg 241
Query: 392 gccttgctgtgctggtgctg 411
|||||||| ||||| |||||
Sbjct: 240 gccttgctctgctgctgctg 221
Score = 56.0 bits (28), Expect = 3e-006
Identities = 55/63 (87%), Gaps = 2/63 (3%)
Strand = Plus / Minus
Query: 122 aacgtctctgtgtaatgtcaagctagctacgtacgccctttattagtcttgtctaatatt 181
||||||||||| ||| ||||| |||||||||||||||| | ||| ||||||||||| ||
Sbjct: 548 aacgtctctgtataacgtcaaactagctacgtacgcccat--ttactcttgtctaatctt 491
Query: 182 ttt 184
|||
Sbjct: 490 ttt 488
>gb|CW110059.1|CW110059 104_482_11103476_148_34514_070 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11103476, DNA
sequence
Length = 233
Score = 184 bits (93), Expect = 6e-045
Identities = 174/200 (87%), Gaps = 12/200 (6%)
Strand = Plus / Plus
Query: 215 caccgtacagccagtcttaccgaacgctcggtgactagctagctggcgtggaccatcggg 274
|||| ||||||||||||| ||||| ||||||||||| ||||||||||||||||| ||
Sbjct: 38 caccctacagccagtcttgccgaatgctcggtgact---tagctggcgtggaccatgggc 94
Query: 275 gactgcggcgac---agcaacgccaaggggctggggatggcgaggtgcgaagcggtggtt 331
|||||||||||| ||||||||||||||||||| ||||||||||||||||||
Sbjct: 95 gactgcggcgacgacagcaacgccaaggggctggagatggcgaggtgcgaagc------a 148
Query: 332 gctccccggcgcggcaagctgaggcggaggggccgcgtggccgcccagcgctcggccttg 391
|||||||||||||||||||||||||| | | | |||||||||||||||||||| ||||||
Sbjct: 149 gctccccggcgcggcaagctgaggcgcatgcggcgcgtggccgcccagcgctctgccttg 208
Query: 392 gccttgctgtgctggtgctg 411
|||||||| ||||| |||||
Sbjct: 209 gccttgctctgctgctgctg 228
>gb|CD209097.1|CD209097 HS1_46_G10.b1_A012 Heat-shocked seedlings Sorghum bicolor cDNA
clone HS1_46_G10_A012 3', mRNA sequence
Length = 603
Score = 131 bits (66), Expect = 7e-029
Identities = 126/145 (86%), Gaps = 12/145 (8%)
Strand = Plus / Minus
Query: 215 caccgtacagccagtcttaccgaacgctcggtgactagctagctggcgtggaccatcggg 274
|||| ||||||||||||| ||||| ||||||||||| ||||||||||||||||| ||
Sbjct: 221 caccctacagccagtcttgccgaatgctcggtgact---tagctggcgtggaccatgggc 165
Query: 275 gactgcggcgac---agcaacgccaaggggctggggatggcgaggtgcgaagcggtggtt 331
|||||||||||| ||||||||||||||||||| ||||||||||||||||||
Sbjct: 164 gactgcggcgacgacagcaacgccaaggggctggagatggcgaggtgcgaagc------a 111
Query: 332 gctccccggcgcggcaagctgaggc 356
|||||||||||||||||||||||||
Sbjct: 110 gctccccggcgcggcaagctgaggc 86
Score = 56.0 bits (28), Expect = 3e-006
Identities = 55/63 (87%), Gaps = 2/63 (3%)
Strand = Plus / Minus
Query: 122 aacgtctctgtgtaatgtcaagctagctacgtacgccctttattagtcttgtctaatatt 181
||||||||||| ||| ||||| |||||||||||||||| | ||| ||||||||||| ||
Sbjct: 358 aacgtctctgtataacgtcaaactagctacgtacgcccat--ttactcttgtctaatctt 301
Query: 182 ttt 184
|||
Sbjct: 300 ttt 298
>gb|BZ780700.1|BZ780700 ii19b06.g1 WGS-SbicolorF (DH5a methyl filtered) Sorghum bicolor
genomic clone ii19b06, DNA sequence
Length = 393
Score = 60.0 bits (30), Expect = 2e-007
Identities = 42/46 (91%)
Strand = Plus / Minus
Query: 463 catgttggcgtagcagacgcggaaccagccgggctcgttgcagtgg 508
|||||||||| |||||||||||||||| ||||||||| |||||||
Sbjct: 357 catgttggcgaagcagacgcggaaccacccgggctcgcggcagtgg 312
>gb|CL159238.1|CL159238 104_348_10804110_116_31380_318 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10804110, DNA
sequence
Length = 632
Score = 56.0 bits (28), Expect = 3e-006
Identities = 55/63 (87%), Gaps = 2/63 (3%)
Strand = Plus / Minus
Query: 122 aacgtctctgtgtaatgtcaagctagctacgtacgccctttattagtcttgtctaatatt 181
||||||||||| ||| ||||| |||||||||||||||| | ||| ||||||||||| ||
Sbjct: 115 aacgtctctgtataacgtcaaactagctacgtacgcccat--ttactcttgtctaatctt 58
Query: 182 ttt 184
|||
Sbjct: 57 ttt 55
>gb|CD425602.1|CD425602 SA1_13_B06.b1_A002 Salicylic acid-treated seedlings Sorghum bicolor
cDNA clone SA1_13_B06_A002 3', mRNA sequence
Length = 409
Score = 56.0 bits (28), Expect = 3e-006
Identities = 55/63 (87%), Gaps = 2/63 (3%)
Strand = Plus / Minus
Query: 122 aacgtctctgtgtaatgtcaagctagctacgtacgccctttattagtcttgtctaatatt 181
||||||||||| ||| ||||| |||||||||||||||| | ||| ||||||||||| ||
Sbjct: 199 aacgtctctgtataacgtcaaactagctacgtacgcccat--ttactcttgtctaatctt 142
Query: 182 ttt 184
|||
Sbjct: 141 ttt 139
Score = 54.0 bits (27), Expect = 1e-005
Identities = 56/65 (86%), Gaps = 3/65 (4%)
Strand = Plus / Minus
Query: 215 caccgtacagccagtcttaccgaacgctcggtgactagctagctggcgtggaccatcggg 274
|||| ||||||||||||| ||||| ||||||||||| || |||||||||||||| ||
Sbjct: 62 caccctacagccagtcttgccgaatgctcggtgact---taactggcgtggaccatgggc 6
Query: 275 gactg 279
|||||
Sbjct: 5 gactg 1
>gb|CD425910.1|CD425910 SA1_15_B06.b1_A002 Salicylic acid-treated seedlings Sorghum bicolor
cDNA clone SA1_15_B06_A002 3', mRNA sequence
Length = 409
Score = 54.0 bits (27), Expect = 1e-005
Identities = 56/65 (86%), Gaps = 3/65 (4%)
Strand = Plus / Minus
Query: 215 caccgtacagccagtcttaccgaacgctcggtgactagctagctggcgtggaccatcggg 274
|||| ||||||||||||| ||||| ||||||||||| || |||||||||||||| ||
Sbjct: 62 caccctacagccagtcttgccgaatgctcggtgact---taactggcgtggaccatgggc 6
Query: 275 gactg 279
|||||
Sbjct: 5 gactg 1
Score = 52.0 bits (26), Expect = 5e-005
Identities = 35/38 (92%)
Strand = Plus / Minus
Query: 122 aacgtctctgtgtaatgtcaagctagctacgtacgccc 159
||||||||||| ||| ||||| ||||||||||||||||
Sbjct: 199 aacgtctctgtataacgtcaaactagctacgtacgccc 162
>gb|CW371298.1|CW371298 fsbb001f046l04f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f046l04, DNA
sequence
Length = 640
Score = 52.0 bits (26), Expect = 5e-005
Identities = 32/34 (94%)
Strand = Plus / Minus
Query: 463 catgttggcgtagcagacgcggaaccagccgggc 496
|||||||||| |||||||||||||||| ||||||
Sbjct: 34 catgttggcgaagcagacgcggaaccacccgggc 1
>gb|CX613321.1|CX613321 GABR1_7_B08.g1_A002 GA- or brassinolide-treated seedlings Sorghum
bicolor cDNA clone GABR1_7_B08_A002 5', mRNA sequence
Length = 728
Score = 40.1 bits (20), Expect = 0.21
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 447 ccatggtgtcgtcgtccatg 466
||||||||||||||||||||
Sbjct: 26 ccatggtgtcgtcgtccatg 45
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 131,331
Number of Sequences: 832831
Number of extensions: 131331
Number of successful extensions: 38277
Number of sequences better than 0.5: 10
Number of HSP's better than 0.5 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 38234
Number of HSP's gapped (non-prelim): 25
length of query: 543
length of database: 491,359,669
effective HSP length: 19
effective length of query: 524
effective length of database: 475,535,880
effective search space: 249180801120
effective search space used: 249180801120
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)