BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521918.2.1
(417 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD428352.1|CD428352 ETH1_31_F04.b1_A002 Ethylene-treated... 377 e-103
gb|CN141083.1|CN141083 OX1_49_E07.b1_A002 Oxidatively-stres... 351 3e-095
gb|AW747493.1|AW747493 WS1_68_E01.g1_A002 Water-stressed 1 ... 256 1e-066
gb|CL149549.1|CL149549 104_330_10777899_116_31354_219 Sorgh... 204 5e-051
gb|CW399413.1|CW399413 fsbb001f089d24f0 Sorghum methylation... 204 5e-051
gb|CF487356.1|CF487356 POL1_43_G03.b1_A002 Pollen Sorghum b... 74 1e-011
gb|CD428460.1|CD428460 ETH1_31_F04.g1_A002 Ethylene-treated... 62 4e-008
gb|CL160593.1|CL160593 104_351_10805102_116_31825_158 Sorgh... 50 2e-004
gb|DV162795.1|DV162795 P1-M15 Sorghum bicolor greenbug-resp... 50 2e-004
gb|DV162820.1|DV162820 P3-G6 Sorghum bicolor greenbug-respo... 50 2e-004
gb|DV162819.2|DV162819 P5-B11 Sorghum bicolor greenbug-resp... 50 2e-004
gb|DR831552.2|DR831552 MT106 Sorghum bicolor greenbug-respo... 46 0.003
gb|CF675626.1|CF675626 THAU2 Subtractive cDNA library from ... 44 0.010
gb|DV162819.1|DV162819 P5-B11 Sorghum bicolor greenbug-resp... 44 0.010
gb|DV162821.1|DV162821 P2-F11 Sorghum bicolor greenbug-resp... 44 0.010
gb|DV162821.2|DV162821 P2-F11 Sorghum bicolor greenbug-resp... 44 0.010
gb|CW106308.1|CW106308 104_475_11013343_116_34456_018 Sorgh... 42 0.040
gb|CF480359.1|CF480359 POL1_65_G03.b1_A002 Pollen Sorghum b... 42 0.040
gb|CF490028.1|CF490028 POL1_62_B10.b1_A002 Pollen Sorghum b... 42 0.040
gb|CF675675.1|CF675675 RUBISCO Subtractive cDNA library fro... 40 0.16
gb|DR831561.1|DR831561 MT14 Sorghum bicolor greenbug-respon... 40 0.16
>gb|CD428352.1|CD428352 ETH1_31_F04.b1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA
clone ETH1_31_F04_A002 3', mRNA sequence
Length = 643
Score = 377 bits (190), Expect = e-103
Identities = 271/299 (90%), Gaps = 4/299 (1%)
Strand = Plus / Plus
Query: 1 ctgcgtgttccgggtttctggccccaagtttctcaagctgaacaaggggacctggcatgc 60
|||||||||||||||||| ||| |||||||||||||||||||||||||||||||||||||
Sbjct: 289 ctgcgtgttccgggtttcaggctccaagtttctcaagctgaacaaggggacctggcatgc 348
Query: 61 cgggcctttgttcaaggcggacgccgttgatttctataatctggaactgagcaacaccaa 120
||||||||||||||||||||| || |||||||||||||||||||| ||||||||||||||
Sbjct: 349 cgggcctttgttcaaggcggatgctgttgatttctataatctggagctgagcaacaccaa 408
Query: 121 cgttgttgatcataccacacaccatttcaagaaacatgatgggataacatttgtggtgga 180
|||||| |||||||| |||||| |||||||||||||||||||||||||||||||||||||
Sbjct: 409 cgttgtcgatcatactacacactatttcaagaaacatgatgggataacatttgtggtgga 468
Query: 181 ggactnnnnnnngaacatctctcaatccgaataccactgacaagaccgctggctgatctt 240
||||| ||||| || ||||||||| | || || |||||| |||||||||||||
Sbjct: 469 ggactaaaa----aacatttcgcaatccgaacatcattgccaagactgctggctgatctt 524
Query: 241 gtccatgtttgtagtgaacaaccaacatgtaaagtgtcatgtagaaatgcgcatctgag 299
| || ||||||||||||||||||||||||||||||| |||||||||||| ||||||||
Sbjct: 525 ctacaagtttgtagtgaacaaccaacatgtaaagtgttatgtagaaatgcacatctgag 583
>gb|CN141083.1|CN141083 OX1_49_E07.b1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_49_E07_A002 3', mRNA sequence
Length = 691
Score = 351 bits (177), Expect = 3e-095
Identities = 267/298 (89%), Gaps = 4/298 (1%)
Strand = Plus / Plus
Query: 2 tgcgtgttccgggtttctggccccaagtttctcaagctgaacaaggggacctggcatgcc 61
||||||||||||||||| ||| ||||||||||||||||||||||||||||||||||||||
Sbjct: 237 tgcgtgttccgggtttcaggctccaagtttctcaagctgaacaaggggacctggcatgcc 296
Query: 62 gggcctttgttcaaggcggacgccgttgatttctataatctggaactgagcaacaccaac 121
|||||||||||||||||||| || |||||||||||||||||||| |||||||||||||||
Sbjct: 297 gggcctttgttcaaggcggatgctgttgatttctataatctggagctgagcaacaccaac 356
Query: 122 gttgttgatcataccacacaccatttcaagaaacatgatgggataacatttgtggtggag 181
||||| |||||||| |||||| ||||||||||||||||||||||||||||||||||||||
Sbjct: 357 gttgtcgatcatactacacactatttcaagaaacatgatgggataacatttgtggtggag 416
Query: 182 gactnnnnnnngaacatctctcaatccgaataccactgacaagaccgctggctgatcttg 241
|||| ||| | || ||||||||| | || || |||||| |||||||||| ||
Sbjct: 417 gactaaaa----aacgtttcgcaatccgaacatcattgccaagactgctggctgattttt 472
Query: 242 tccatgtttgtagtgaacaaccaacatgtaaagtgtcatgtagaaatgcgcatctgag 299
| || ||||||||||||||||||||||||||||||| |||||||||||| ||| ||||
Sbjct: 473 tacaagtttgtagtgaacaaccaacatgtaaagtgttatgtagaaatgcacatttgag 530
>gb|AW747493.1|AW747493 WS1_68_E01.g1_A002 Water-stressed 1 (WS1) Sorghum bicolor cDNA,
mRNA sequence
Length = 218
Score = 256 bits (129), Expect = 1e-066
Identities = 168/181 (92%)
Strand = Plus / Plus
Query: 5 gtgttccgggtttctggccccaagtttctcaagctgaacaaggggacctggcatgccggg 64
|||||||||||||| ||| |||||||||||||| ||||||||||||||||||||||||||
Sbjct: 1 gtgttccgggtttcaggctccaagtttctcaagttgaacaaggggacctggcatgccggg 60
Query: 65 cctttgttcaaggcggacgccgttgatttctataatctggaactgagcaacaccaacgtt 124
||||||||||||||||| || |||||||||||||||| ||| |||||||||||||||||
Sbjct: 61 cctttgttcaaggcggatgctgttgatttctataatcgggagttgagcaacaccaacgtt 120
Query: 125 gttgatcataccacacaccatttcaagaaacatgatgggataacatttgtggtggaggac 184
|| ||||||| |||| | |||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 gtcgatcatattacacgctatttcaagaaacatgatgggataacatttgtggtggaggac 180
Query: 185 t 185
|
Sbjct: 181 t 181
>gb|CL149549.1|CL149549 104_330_10777899_116_31354_219 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10777899, DNA
sequence
Length = 743
Score = 204 bits (103), Expect = 5e-051
Identities = 118/123 (95%)
Strand = Plus / Minus
Query: 1 ctgcgtgttccgggtttctggccccaagtttctcaagctgaacaaggggacctggcatgc 60
|||||||||||||||||| ||| |||||||||||||||||||||||||||||||||||||
Sbjct: 593 ctgcgtgttccgggtttcaggctccaagtttctcaagctgaacaaggggacctggcatgc 534
Query: 61 cgggcctttgttcaaggcggacgccgttgatttctataatctggaactgagcaacaccaa 120
||||||||||||||||||||| || |||||||||||||||||||| ||||||||||||||
Sbjct: 533 cgggcctttgttcaaggcggatgctgttgatttctataatctggagctgagcaacaccaa 474
Query: 121 cgt 123
|||
Sbjct: 473 cgt 471
>gb|CW399413.1|CW399413 fsbb001f089d24f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f089d24, DNA
sequence
Length = 674
Score = 204 bits (103), Expect = 5e-051
Identities = 118/123 (95%)
Strand = Plus / Minus
Query: 1 ctgcgtgttccgggtttctggccccaagtttctcaagctgaacaaggggacctggcatgc 60
|||||||||||||||||| ||| |||||||||||||||||||||||||||||||||||||
Sbjct: 496 ctgcgtgttccgggtttcaggctccaagtttctcaagctgaacaaggggacctggcatgc 437
Query: 61 cgggcctttgttcaaggcggacgccgttgatttctataatctggaactgagcaacaccaa 120
||||||||||||||||||||| || |||||||||||||||||||| ||||||||||||||
Sbjct: 436 cgggcctttgttcaaggcggatgctgttgatttctataatctggagctgagcaacaccaa 377
Query: 121 cgt 123
|||
Sbjct: 376 cgt 374
>gb|CF487356.1|CF487356 POL1_43_G03.b1_A002 Pollen Sorghum bicolor cDNA clone
POL1_43_G03_A002 3', mRNA sequence
Length = 221
Score = 73.8 bits (37), Expect = 1e-011
Identities = 49/53 (92%)
Strand = Plus / Plus
Query: 247 gtttgtagtgaacaaccaacatgtaaagtgtcatgtagaaatgcgcatctgag 299
|||||||||||||||||| |||||||||||| |||||||||||| ||| ||||
Sbjct: 12 gtttgtagtgaacaaccatcatgtaaagtgttatgtagaaatgcacatttgag 64
>gb|CD428460.1|CD428460 ETH1_31_F04.g1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA
clone ETH1_31_F04_A002 5', mRNA sequence
Length = 607
Score = 61.9 bits (31), Expect = 4e-008
Identities = 37/39 (94%)
Strand = Plus / Plus
Query: 1 ctgcgtgttccgggtttctggccccaagtttctcaagct 39
|||||||||||||||||| ||| ||||||||||||||||
Sbjct: 569 ctgcgtgttccgggtttcaggctccaagtttctcaagct 607
>gb|CL160593.1|CL160593 104_351_10805102_116_31825_158 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10805102, DNA
sequence
Length = 738
Score = 50.1 bits (25), Expect = 2e-004
Identities = 37/41 (90%)
Strand = Plus / Minus
Query: 123 ttgttgatcataccacacaccatttcaagaaacatgatggg 163
|||||||||| || |||||||||||| |||||||||||||
Sbjct: 183 ttgttgatcacacatcacaccatttcaggaaacatgatggg 143
>gb|DV162795.1|DV162795 P1-M15 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 282
Score = 50.1 bits (25), Expect = 2e-004
Identities = 25/25 (100%)
Strand = Plus / Minus
Query: 393 gtacctgcccgggcggccgctcgaa 417
|||||||||||||||||||||||||
Sbjct: 51 gtacctgcccgggcggccgctcgaa 27
>gb|DV162820.1|DV162820 P3-G6 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 223
Score = 50.1 bits (25), Expect = 2e-004
Identities = 25/25 (100%)
Strand = Plus / Plus
Query: 393 gtacctgcccgggcggccgctcgaa 417
|||||||||||||||||||||||||
Sbjct: 100 gtacctgcccgggcggccgctcgaa 124
>gb|DV162819.2|DV162819 P5-B11 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 172
Score = 50.1 bits (25), Expect = 2e-004
Identities = 25/25 (100%)
Strand = Plus / Plus
Query: 393 gtacctgcccgggcggccgctcgaa 417
|||||||||||||||||||||||||
Sbjct: 2 gtacctgcccgggcggccgctcgaa 26
>gb|DR831552.2|DR831552 MT106 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 253
Score = 46.1 bits (23), Expect = 0.003
Identities = 23/23 (100%)
Strand = Plus / Plus
Query: 395 acctgcccgggcggccgctcgaa 417
|||||||||||||||||||||||
Sbjct: 197 acctgcccgggcggccgctcgaa 219
>gb|CF675626.1|CF675626 THAU2 Subtractive cDNA library from sorghum infested by greenbug
aphids Sorghum bicolor cDNA clone THAU2 similar to
Thaumatin-like protein, mRNA sequence
Length = 747
Score = 44.1 bits (22), Expect = 0.010
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 391 cagtacctgcccgggcggccgc 412
||||||||||||||||||||||
Sbjct: 190 cagtacctgcccgggcggccgc 211
Score = 40.1 bits (20), Expect = 0.16
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 393 gtacctgcccgggcggccgc 412
||||||||||||||||||||
Sbjct: 223 gtacctgcccgggcggccgc 204
>gb|DV162819.1|DV162819 P5-B11 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 234
Score = 44.1 bits (22), Expect = 0.010
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 393 gtacctgcccgggcggccgctc 414
||||||||||||||||||||||
Sbjct: 65 gtacctgcccgggcggccgctc 86
>gb|DV162821.1|DV162821 P2-F11 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 231
Score = 44.1 bits (22), Expect = 0.010
Identities = 22/22 (100%)
Strand = Plus / Minus
Query: 396 cctgcccgggcggccgctcgaa 417
||||||||||||||||||||||
Sbjct: 114 cctgcccgggcggccgctcgaa 93
>gb|DV162821.2|DV162821 P2-F11 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 214
Score = 44.1 bits (22), Expect = 0.010
Identities = 22/22 (100%)
Strand = Plus / Minus
Query: 396 cctgcccgggcggccgctcgaa 417
||||||||||||||||||||||
Sbjct: 97 cctgcccgggcggccgctcgaa 76
>gb|CW106308.1|CW106308 104_475_11013343_116_34456_018 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11013343, DNA
sequence
Length = 700
Score = 42.1 bits (21), Expect = 0.040
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 108 tgagcaacaccaacgttgttg 128
|||||||||||||||||||||
Sbjct: 430 tgagcaacaccaacgttgttg 450
>gb|CF480359.1|CF480359 POL1_65_G03.b1_A002 Pollen Sorghum bicolor cDNA clone
POL1_65_G03_A002 3', mRNA sequence
Length = 712
Score = 42.1 bits (21), Expect = 0.040
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 108 tgagcaacaccaacgttgttg 128
|||||||||||||||||||||
Sbjct: 313 tgagcaacaccaacgttgttg 293
>gb|CF490028.1|CF490028 POL1_62_B10.b1_A002 Pollen Sorghum bicolor cDNA clone
POL1_62_B10_A002 3', mRNA sequence
Length = 642
Score = 42.1 bits (21), Expect = 0.040
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 108 tgagcaacaccaacgttgttg 128
|||||||||||||||||||||
Sbjct: 227 tgagcaacaccaacgttgttg 207
>gb|CF675675.1|CF675675 RUBISCO Subtractive cDNA library from sorghum infested by greenbug
aphids Sorghum bicolor cDNA clone RUBISCO similar to
Ribulose 1,5-biphosphate carboxylase, mRNA sequence
Length = 638
Score = 40.1 bits (20), Expect = 0.16
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 393 gtacctgcccgggcggccgc 412
||||||||||||||||||||
Sbjct: 244 gtacctgcccgggcggccgc 225
>gb|DR831561.1|DR831561 MT14 Sorghum bicolor greenbug-responsive cDNA library Sorghum
bicolor cDNA 5', mRNA sequence
Length = 112
Score = 40.1 bits (20), Expect = 0.16
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 393 gtacctgcccgggcggccgc 412
||||||||||||||||||||
Sbjct: 21 gtacctgcccgggcggccgc 2
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 93,102
Number of Sequences: 832831
Number of extensions: 93102
Number of successful extensions: 23523
Number of sequences better than 0.5: 21
Number of HSP's better than 0.5 without gapping: 21
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 23493
Number of HSP's gapped (non-prelim): 30
length of query: 417
length of database: 491,359,669
effective HSP length: 19
effective length of query: 398
effective length of database: 475,535,880
effective search space: 189263280240
effective search space used: 189263280240
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)