BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAZ3f05.yg.3.1
(1626 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU896326.1|BU896326 X039B02 Populus wood cDNA library Po... 80 3e-013
gb|CX167513.1|CX167513 C05_69-3_05.ab1 leaf inoculated with... 72 7e-011
gb|CX176911.1|CX176911 A05_69-119_01.ab1 leaf inoculated wi... 64 2e-008
gb|BU821454.1|BU821454 UB23CPD09 Populus tremula cambium cD... 60 3e-007
gb|BU830332.1|BU830332 T007A06 Populus apical shoot cDNA li... 60 3e-007
gb|CK096692.1|CK096692 UB23CPD09.3pR Populus active cambium... 60 3e-007
gb|CX659218.1|CX659218 PO01027H09 Poplar SC cDNA library Po... 60 3e-007
gb|CV225794.1|CV225794 WS0162.B21_G17 PT-DX-A-7 Populus tri... 58 1e-006
gb|DV465740.1|DV465740 MTUNUL1.P42.E10 NUL Populus fremonti... 58 1e-006
gb|DN490148.1|DN490148 UB43DPE12.3pR Populus active cambium... 54 2e-005
gb|DN500119.1|DN500119 UB43DPE12.5pR Populus active cambium... 54 2e-005
gb|DT471997.1|DT471997 WS0122.BR_M05 PT-GT-FL-A-3 Populus t... 54 2e-005
gb|BU813620.1|BU813620 N012G06 Populus bark cDNA library Po... 52 7e-005
gb|CK090784.1|CK090784 F024P68.3pR Populus flower cDNA libr... 50 3e-004
gb|CN523483.1|CN523483 GQ015M02.T3_F09 GQ015 Populus tricho... 50 3e-004
gb|DT479417.1|DT479417 WS02525.BR_M22 PT-MB-N-A-15 Populus ... 50 3e-004
gb|DT484576.1|DT484576 WS02525.B21_M22 PT-MB-N-A-15 Populus... 50 3e-004
gb|BU822796.1|BU822796 UB43DPE12 Populus tremula cambium cD... 46 0.004
gb|CA923573.1|CA923573 MTU7CL.P12.B05 Aspen leaf cDNA Libra... 46 0.004
gb|CV236725.1|CV236725 WS01225.B21.1_C23 PT-GT-FL-A-3 Popul... 46 0.004
gb|CX659359.1|CX659359 PO01029G04 Poplar SC cDNA library Po... 44 0.017
gb|CA926324.1|CA926324 MTU6CR.P13.F09 Aspen root cDNA Libra... 40 0.26
gb|CV268519.1|CV268519 WS0205.B21_L11 PTxN-IB-N-A-11 Populu... 40 0.26
gb|CV279676.1|CV279676 WS0132.B21_N13 PTxD-IL-FL-A-4 Populu... 40 0.26
gb|BP933906.1|BP933906 BP933906 full-length enriched poplar... 40 0.26
gb|BP934434.1|BP934434 BP934434 full-length enriched poplar... 40 0.26
gb|DT517734.1|DT517734 WS02435.B21_C12 PTxD-ICC-N-A-14 Popu... 40 0.26
>gb|BU896326.1|BU896326 X039B02 Populus wood cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 736
Score = 79.8 bits (40), Expect = 3e-013
Identities = 106/128 (82%)
Strand = Plus / Plus
Query: 411 tttatagttgggcccataccagtagatgatggaaatggaaaagaagtagcaactgaaatt 470
||||| |||||||||||||| | || ||||||| |||||||||||| |||||| ||||
Sbjct: 29 tttatcgttgggcccatacccattgaagatggaattggaaaagaagttgcaactcaaatc 88
Query: 471 gttacaaacatggtgacaaacaaaacattctacactgattccagtggacgtgatttcatc 530
|||| ||||| || || |||||||| ||||| ||||| | |||||||||||| |||
Sbjct: 89 acaacaaccatggaaaccaataaaacattttacacagattctaatggacgtgattttatc 148
Query: 531 aaaaggat 538
||||||||
Sbjct: 149 aaaaggat 156
Score = 60.0 bits (30), Expect = 3e-007
Identities = 63/74 (85%)
Strand = Plus / Plus
Query: 1005 caggagcttgaagatggaagtgttctccttcggtttgctcatctatatgaggccggggag 1064
||||||||||| ||||||| |||||||| || || |||||| |||||||| || |||
Sbjct: 623 caggagcttgatgatggaaaagttctcctccgtttagctcatttatatgagatgggagag 682
Query: 1065 gacaaggatctttc 1078
||||||||||||||
Sbjct: 683 gacaaggatctttc 696
Score = 42.1 bits (21), Expect = 0.066
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 686 ggatggacaaatagagctaatgctacacaggag 718
|||||||||||||||||| ||||| || |||||
Sbjct: 304 ggatggacaaatagagctgatgctccataggag 336
>gb|CX167513.1|CX167513 C05_69-3_05.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 623
Score = 71.9 bits (36), Expect = 7e-011
Identities = 48/52 (92%)
Strand = Plus / Plus
Query: 324 tattggatgaagtgcaccagcagataaattcatggatttatcagattaccag 375
||||||||||||||||||||||||| | ||||||||| |||||||| |||||
Sbjct: 81 tattggatgaagtgcaccagcagatcagttcatggatatatcagataaccag 132
>gb|CX176911.1|CX176911 A05_69-119_01.ab1 leaf inoculated with Marssonia pathogen of
Populus deltoides Populus deltoides cDNA, mRNA sequence
Length = 682
Score = 63.9 bits (32), Expect = 2e-008
Identities = 47/52 (90%)
Strand = Plus / Plus
Query: 324 tattggatgaagtgcaccagcagataaattcatggatttatcagattaccag 375
||||||||||||||||||||||||| | ||| ||||| |||||||| |||||
Sbjct: 186 tattggatgaagtgcaccagcagatcagttcgtggatatatcagataaccag 237
Score = 46.1 bits (23), Expect = 0.004
Identities = 35/39 (89%)
Strand = Plus / Plus
Query: 233 caggcttctggagcatacatattccgccctaatggcaca 271
||||| |||||||||||||| ||||| || |||||||||
Sbjct: 95 caggcctctggagcatacatcttccgaccaaatggcaca 133
>gb|BU821454.1|BU821454 UB23CPD09 Populus tremula cambium cDNA library Populus tremula cDNA 5
prime, mRNA sequence
Length = 441
Score = 60.0 bits (30), Expect = 3e-007
Identities = 63/74 (85%)
Strand = Plus / Plus
Query: 1005 caggagcttgaagatggaagtgttctccttcggtttgctcatctatatgaggccggggag 1064
||||||||||| ||||||| |||||||| || || |||||| |||||||| || |||
Sbjct: 243 caggagcttgacgatggaaaagttctcctccgtttagctcatttatatgagatgggagag 302
Query: 1065 gacaaggatctttc 1078
||||||||||||||
Sbjct: 303 gacaaggatctttc 316
>gb|BU830332.1|BU830332 T007A06 Populus apical shoot cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 382
Score = 60.0 bits (30), Expect = 3e-007
Identities = 63/74 (85%)
Strand = Plus / Plus
Query: 1005 caggagcttgaagatggaagtgttctccttcggtttgctcatctatatgaggccggggag 1064
||||||||||| ||||||| |||||||| || || |||||| |||||||| || |||
Sbjct: 150 caggagcttgatgatggaaaagttctcctccgtttagctcatttatatgagatgggagag 209
Query: 1065 gacaaggatctttc 1078
||||||||||||||
Sbjct: 210 gacaaggatctttc 223
>gb|CK096692.1|CK096692 UB23CPD09.3pR Populus active cambium cDNA library Populus tremula
cDNA clone UB23CPD09 3', mRNA sequence
Length = 854
Score = 60.0 bits (30), Expect = 3e-007
Identities = 63/74 (85%)
Strand = Plus / Minus
Query: 1005 caggagcttgaagatggaagtgttctccttcggtttgctcatctatatgaggccggggag 1064
||||||||||| ||||||| |||||||| || || |||||| |||||||| || |||
Sbjct: 722 caggagcttgacgatggaaaagttctcctccgtttagctcatttatatgagatgggagag 663
Query: 1065 gacaaggatctttc 1078
||||||||||||||
Sbjct: 662 gacaaggatctttc 649
>gb|CX659218.1|CX659218 PO01027H09 Poplar SC cDNA library Populus alba x Populus tremula var.
glandulosa cDNA clone PO01027H09 5', mRNA sequence
Length = 744
Score = 60.0 bits (30), Expect = 3e-007
Identities = 63/74 (85%)
Strand = Plus / Plus
Query: 1005 caggagcttgaagatggaagtgttctccttcggtttgctcatctatatgaggccggggag 1064
||||||||||| ||||||| |||||||| || || |||||| |||||||| || |||
Sbjct: 69 caggagcttgatgatggaaaagttctcctccgtttagctcatttatatgagatgggagag 128
Query: 1065 gacaaggatctttc 1078
||||||||||||||
Sbjct: 129 gacaaggatctttc 142
>gb|CV225794.1|CV225794 WS0162.B21_G17 PT-DX-A-7 Populus trichocarpa cDNA clone WS0162_G17
3', mRNA sequence
Length = 757
Score = 58.0 bits (29), Expect = 1e-006
Identities = 41/45 (91%)
Strand = Plus / Minus
Query: 1029 ctccttcggtttgctcatctatatgaggccggggaggacaaggat 1073
||||||||||| ||||| || |||||| |||||||||||||||||
Sbjct: 482 ctccttcggttggctcacctttatgagaccggggaggacaaggat 438
Score = 40.1 bits (20), Expect = 0.26
Identities = 32/36 (88%)
Strand = Plus / Minus
Query: 851 gtggcgtcggacatttggccaggaaatatactcccc 886
||||||||| ||||||||||| || ||||| |||||
Sbjct: 660 gtggcgtcgaacatttggccaagagatatattcccc 625
>gb|DV465740.1|DV465740 MTUNUL1.P42.E10 NUL Populus fremontii x Populus angustifolia cDNA,
mRNA sequence
Length = 807
Score = 58.0 bits (29), Expect = 1e-006
Identities = 41/45 (91%)
Strand = Plus / Plus
Query: 1029 ctccttcggtttgctcatctatatgaggccggggaggacaaggat 1073
||||||||||| ||||| || |||||| |||||||||||||||||
Sbjct: 632 ctccttcggttggctcacctttatgagaccggggaggacaaggat 676
Score = 40.1 bits (20), Expect = 0.26
Identities = 32/36 (88%)
Strand = Plus / Plus
Query: 851 gtggcgtcggacatttggccaggaaatatactcccc 886
||||||||| ||||||||||| || ||||| |||||
Sbjct: 454 gtggcgtcgaacatttggccaagagatatattcccc 489
>gb|DN490148.1|DN490148 UB43DPE12.3pR Populus active cambium cDNA library Populus tremula
cDNA clone UB43DPE12 3', mRNA sequence
Length = 588
Score = 54.0 bits (27), Expect = 2e-005
Identities = 51/59 (86%)
Strand = Plus / Minus
Query: 1017 gatggaagtgttctccttcggtttgctcatctatatgaggccggggaggacaaggatct 1075
||||||| |||||||||||||| || || ||||||||| |||||||||||||||||
Sbjct: 506 gatggaaaggttctccttcggttagcacacttatatgaggttggggaggacaaggatct 448
>gb|DN500119.1|DN500119 UB43DPE12.5pR Populus active cambium cDNA library Populus tremula
cDNA clone UB43DPE12 5', mRNA sequence
Length = 591
Score = 54.0 bits (27), Expect = 2e-005
Identities = 51/59 (86%)
Strand = Plus / Plus
Query: 1017 gatggaagtgttctccttcggtttgctcatctatatgaggccggggaggacaaggatct 1075
||||||| |||||||||||||| || || ||||||||| |||||||||||||||||
Sbjct: 79 gatggaaaggttctccttcggttagcacacttatatgaggttggggaggacaaggatct 137
>gb|DT471997.1|DT471997 WS0122.BR_M05 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS0122_M05
5', mRNA sequence
Length = 561
Score = 54.0 bits (27), Expect = 2e-005
Identities = 51/59 (86%)
Strand = Plus / Plus
Query: 1017 gatggaagtgttctccttcggtttgctcatctatatgaggccggggaggacaaggatct 1075
||||||| |||||||||||||| || || ||||||||| |||||||||||||||||
Sbjct: 159 gatggaaaggttctccttcggttagcacacttatatgaggttggggaggacaaggatct 217
>gb|BU813620.1|BU813620 N012G06 Populus bark cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 509
Score = 52.0 bits (26), Expect = 7e-005
Identities = 62/74 (83%)
Strand = Plus / Plus
Query: 1005 caggagcttgaagatggaagtgttctccttcggtttgctcatctatatgaggccggggag 1064
||||||||||| ||||||| | |||||| || || |||||| |||||||| || |||
Sbjct: 371 caggagcttgatgatggaaaagctctcctccgtttagctcatttatatgagatgggagag 430
Query: 1065 gacaaggatctttc 1078
||||||||||||||
Sbjct: 431 gacaaggatctttc 444
Score = 42.1 bits (21), Expect = 0.066
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 686 ggatggacaaatagagctaatgctacacaggag 718
|||||||||||||||||| ||||| || |||||
Sbjct: 52 ggatggacaaatagagctgatgctccataggag 84
>gb|CK090784.1|CK090784 F024P68.3pR Populus flower cDNA library Populus trichocarpa cDNA
clone F024P68 3', mRNA sequence
Length = 821
Score = 50.1 bits (25), Expect = 3e-004
Identities = 40/45 (88%)
Strand = Plus / Minus
Query: 1029 ctccttcggtttgctcatctatatgaggccggggaggacaaggat 1073
||||||||||| ||||| || |||||| |||| ||||||||||||
Sbjct: 483 ctccttcggttggctcacctttatgagaccggtgaggacaaggat 439
Score = 46.1 bits (23), Expect = 0.004
Identities = 44/51 (86%)
Strand = Plus / Minus
Query: 836 aggagaaggagctcggtggcgtcggacatttggccaggaaatatactcccc 886
||||||||| || |||||||||| ||||||||||| || ||||| |||||
Sbjct: 676 aggagaaggtgccaggtggcgtcgaacatttggccaagagatatattcccc 626
>gb|CN523483.1|CN523483 GQ015M02.T3_F09 GQ015 Populus trichocarpa x Populus deltoides cDNA
clone GQ015M02_F09 5', mRNA sequence
Length = 770
Score = 50.1 bits (25), Expect = 3e-004
Identities = 40/45 (88%)
Strand = Plus / Plus
Query: 1029 ctccttcggtttgctcatctatatgaggccggggaggacaaggat 1073
||||||||||| ||||| || |||||| |||| ||||||||||||
Sbjct: 339 ctccttcggttggctcacctttatgagaccggtgaggacaaggat 383
Score = 46.1 bits (23), Expect = 0.004
Identities = 44/51 (86%)
Strand = Plus / Plus
Query: 836 aggagaaggagctcggtggcgtcggacatttggccaggaaatatactcccc 886
||||||||| || |||||||||| ||||||||||| || ||||| |||||
Sbjct: 146 aggagaaggtgccaggtggcgtcgaacatttggccaagagatatattcccc 196
>gb|DT479417.1|DT479417 WS02525.BR_M22 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02525_M22 5', mRNA sequence
Length = 793
Score = 50.1 bits (25), Expect = 3e-004
Identities = 40/45 (88%)
Strand = Plus / Plus
Query: 1029 ctccttcggtttgctcatctatatgaggccggggaggacaaggat 1073
||||||||||| ||||| || |||||| |||| ||||||||||||
Sbjct: 301 ctccttcggttggctcacctttatgagaccggtgaggacaaggat 345
Score = 46.1 bits (23), Expect = 0.004
Identities = 44/51 (86%)
Strand = Plus / Plus
Query: 836 aggagaaggagctcggtggcgtcggacatttggccaggaaatatactcccc 886
||||||||| || |||||||||| ||||||||||| || ||||| |||||
Sbjct: 108 aggagaaggtgccaggtggcgtcgaacatttggccaagagatatattcccc 158
>gb|DT484576.1|DT484576 WS02525.B21_M22 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02525_M22 3', mRNA sequence
Length = 780
Score = 50.1 bits (25), Expect = 3e-004
Identities = 40/45 (88%)
Strand = Plus / Minus
Query: 1029 ctccttcggtttgctcatctatatgaggccggggaggacaaggat 1073
||||||||||| ||||| || |||||| |||| ||||||||||||
Sbjct: 720 ctccttcggttggctcacctttatgagaccggtgaggacaaggat 676
>gb|BU822796.1|BU822796 UB43DPE12 Populus tremula cambium cDNA library Populus tremula cDNA 5
prime, mRNA sequence
Length = 423
Score = 46.1 bits (23), Expect = 0.004
Identities = 41/47 (87%)
Strand = Plus / Plus
Query: 1029 ctccttcggtttgctcatctatatgaggccggggaggacaaggatct 1075
||||||||||| || || ||||||||| |||||||||||||||||
Sbjct: 95 ctccttcggttagcacacttatatgaggttggggaggacaaggatct 141
>gb|CA923573.1|CA923573 MTU7CL.P12.B05 Aspen leaf cDNA Library Populus tremuloides cDNA, mRNA
sequence
Length = 480
Score = 46.1 bits (23), Expect = 0.004
Identities = 50/59 (84%)
Strand = Plus / Minus
Query: 1017 gatggaagtgttctccttcggtttgctcatctatatgaggccggggaggacaaggatct 1075
||||||| |||||||||||| | || || ||||||||| |||||||||||||||||
Sbjct: 280 gatggaaaggttctccttcggctagcacacttatatgaggttggggaggacaaggatct 222
>gb|CV236725.1|CV236725 WS01225.B21.1_C23 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01225_C23 3', mRNA sequence
Length = 938
Score = 46.1 bits (23), Expect = 0.004
Identities = 44/51 (86%)
Strand = Plus / Minus
Query: 836 aggagaaggagctcggtggcgtcggacatttggccaggaaatatactcccc 886
||||||||| || |||||||||| ||||||||||| || ||||| |||||
Sbjct: 523 aggagaaggtgccaggtggcgtcgaacatttggccaagagatatattcccc 473
Score = 42.1 bits (21), Expect = 0.066
Identities = 39/45 (86%)
Strand = Plus / Minus
Query: 1029 ctccttcggtttgctcatctatatgaggccggggaggacaaggat 1073
||||||||||| ||||| || ||||| |||| ||||||||||||
Sbjct: 330 ctccttcggttggctcacctttatgaaaccggtgaggacaaggat 286
Score = 40.1 bits (20), Expect = 0.26
Identities = 29/32 (90%)
Strand = Plus / Minus
Query: 582 attgctggaaactattatcctgttaatcttgg 613
||||||||||| ||||| ||||| ||||||||
Sbjct: 777 attgctggaaattattaccctgtaaatcttgg 746
>gb|CX659359.1|CX659359 PO01029G04 Poplar SC cDNA library Populus alba x Populus tremula var.
glandulosa cDNA clone PO01029G04 5', mRNA sequence
Length = 282
Score = 44.1 bits (22), Expect = 0.017
Identities = 61/74 (82%)
Strand = Plus / Plus
Query: 1005 caggagcttgaagatggaagtgttctccttcggtttgctcatctatatgaggccggggag 1064
||||||||||| ||||||| | |||||| || | |||||| |||||||| || |||
Sbjct: 71 caggagcttgatgatggaaaaggtctcctccgttaagctcatttatatgagatgggagag 130
Query: 1065 gacaaggatctttc 1078
||||||||||||||
Sbjct: 131 gacaaggatctttc 144
>gb|CA926324.1|CA926324 MTU6CR.P13.F09 Aspen root cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 579
Score = 40.1 bits (20), Expect = 0.26
Identities = 29/32 (90%)
Strand = Plus / Plus
Query: 582 attgctggaaactattatcctgttaatcttgg 613
||||||||||| ||||| ||||| ||||||||
Sbjct: 243 attgctggaaattattaccctgtaaatcttgg 274
>gb|CV268519.1|CV268519 WS0205.B21_L11 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS0205_L11 3', mRNA sequence
Length = 571
Score = 40.1 bits (20), Expect = 0.26
Identities = 23/24 (95%)
Strand = Plus / Minus
Query: 441 ggaaatggaaaagaagtagcaact 464
|||||||||||||||| |||||||
Sbjct: 368 ggaaatggaaaagaagaagcaact 345
>gb|CV279676.1|CV279676 WS0132.B21_N13 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS0132_N13 3', mRNA sequence
Length = 721
Score = 40.1 bits (20), Expect = 0.26
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 441 ggaaatggaaaagaagtagcaact 464
|||||||||||||||| |||||||
Sbjct: 171 ggaaatggaaaagaagaagcaact 194
>gb|BP933906.1|BP933906 BP933906 full-length enriched poplar cDNA library Populus nigra
cDNA clone PnFL1-058_A13.r 3', mRNA sequence
Length = 593
Score = 40.1 bits (20), Expect = 0.26
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 441 ggaaatggaaaagaagtagcaact 464
|||||||||||||||| |||||||
Sbjct: 155 ggaaatggaaaagaagaagcaact 178
>gb|BP934434.1|BP934434 BP934434 full-length enriched poplar cDNA library Populus nigra
cDNA clone PnFL1-065_F11.r 3', mRNA sequence
Length = 661
Score = 40.1 bits (20), Expect = 0.26
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 441 ggaaatggaaaagaagtagcaact 464
|||||||||||||||| |||||||
Sbjct: 155 ggaaatggaaaagaagaagcaact 178
>gb|DT517734.1|DT517734 WS02435.B21_C12 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02435_C12 3', mRNA sequence
Length = 882
Score = 40.1 bits (20), Expect = 0.26
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 441 ggaaatggaaaagaagtagcaact 464
|||||||||||||||| |||||||
Sbjct: 111 ggaaatggaaaagaagaagcaact 134
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 228,388
Number of Sequences: 369679
Number of extensions: 228388
Number of successful extensions: 67251
Number of sequences better than 0.5: 27
Number of HSP's better than 0.5 without gapping: 27
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 67163
Number of HSP's gapped (non-prelim): 87
length of query: 1626
length of database: 203,408,664
effective HSP length: 19
effective length of query: 1607
effective length of database: 196,384,763
effective search space: 315590314141
effective search space used: 315590314141
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)