BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2418861.2.1
(614 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR691945.1|DR691945 EST1082032 Normalized pine embryo li... 129 1e-028
gb|DT636879.1|DT636879 EST1151810 Normalized pine embryo li... 129 1e-028
dbj|BD273093.1| Materials and methods for the modification ... 129 1e-028
gb|CX713935.1|CX713935 RTPQ1_15_D05.b1_A032 Roots treated w... 121 4e-026
gb|DR693591.1|DR693591 EST1083680 Normalized pine embryo li... 121 4e-026
gb|DR686435.1|DR686435 EST1076513 Normalized pine embryo li... 113 9e-024
gb|BQ698117.1|BQ698117 NXPV_064_G03_F NXPV (Nsf Xylem Plani... 101 3e-020
gb|BQ701207.1|BQ701207 NXSI_023_G09_F NXSI (Nsf Xylem Side ... 101 3e-020
gb|DT633502.1|DT633502 EST1148433 Normalized pine embryo li... 100 1e-019
gb|BQ699341.1|BQ699341 NXRV126_E04_F NXRV (Nsf Xylem Root w... 74 7e-012
gb|BQ700491.1|BQ700491 NXRV107_D05_F NXRV (Nsf Xylem Root w... 66 2e-009
gb|CX645971.1|CX645971 COLD1_6_A08.g1_A029 Root cold Pinus ... 58 4e-007
gb|CF672887.1|CF672887 RTCNT1_74_D06.g1_A029 Root control P... 44 0.007
gb|DR080387.1|DR080387 RTFEPL1_22_G06.b1_A029 Roots plus ad... 40 0.10
gb|DR080460.1|DR080460 RTFEPL1_22_G06.g1_A029 Roots plus ad... 40 0.10
gb|DR110130.1|DR110130 RTS1_9_C04.b1_A029 Roots minus sulfu... 40 0.10
gb|DR110629.1|DR110629 RTS1_12_B03.b1_A029 Roots minus sulf... 40 0.10
gb|DR118894.1|DR118894 RTMG1_19_G09.g1_A029 Roots minus mag... 38 0.41
gb|DR168008.1|DR168008 RTPHOS1_22_C11.g1_A029 Roots minus p... 38 0.41
>gb|DR691945.1|DR691945 EST1082032 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWADG12 3' end, mRNA sequence
Length = 832
Score = 129 bits (65), Expect = 1e-028
Identities = 182/221 (82%)
Strand = Plus / Plus
Query: 237 aactatactgatatggttaataaatactatgatcttgccactagcttctatgagtatggt 296
|||||||| || ||||| ||||||||||||||||| || ||||| ||||||||||||||
Sbjct: 123 aactatacagacatggtaaataaatactatgatctggcgactagtttctatgagtatgga 182
Query: 297 tggggtgaatccttccactttgctcacagatggaatggagaatccttacgtgaaagcatc 356
||||| || || || || ||||||||||||||||| || || || | || ||||| ||
Sbjct: 183 tggggggagtcttttcattttgctcacagatggaaaggggagaccctccgagaaagtata 242
Query: 357 aagcgacatgagcattttcttgccctgcaacttggtttgaaaccaggaatgaaggtttta 416
||||| ||||| ||||||||||| || || ||| |||| || || | ||||||||| ||
Sbjct: 243 aagcgccatgaacattttcttgctcttcagctttgtttaaagcctgcaatgaaggtattg 302
Query: 417 gatgtgggctgtggaataggtggaccactgagagaaattgc 457
||||| || ||||| || || || |||||||||||||||||
Sbjct: 303 gatgttggatgtgggattggaggtccactgagagaaattgc 343
Score = 54.0 bits (27), Expect = 7e-006
Identities = 33/35 (94%)
Strand = Plus / Plus
Query: 555 tgtgattttgtcaaggcggacttcatgaagatgcc 589
|||||||||||||||| |||||||||||||||||
Sbjct: 441 tgtgattttgtcaagggagacttcatgaagatgcc 475
>gb|DT636879.1|DT636879 EST1151810 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMGX11 3' end, mRNA sequence
Length = 829
Score = 129 bits (65), Expect = 1e-028
Identities = 182/221 (82%)
Strand = Plus / Plus
Query: 237 aactatactgatatggttaataaatactatgatcttgccactagcttctatgagtatggt 296
|||||||| || ||||| ||||||||||||||||| || ||||| ||||||||||||||
Sbjct: 131 aactatacagacatggtaaataaatactatgatctggcgactagtttctatgagtatgga 190
Query: 297 tggggtgaatccttccactttgctcacagatggaatggagaatccttacgtgaaagcatc 356
||||| || || || || ||||||||||||||||| || || || | || ||||| ||
Sbjct: 191 tggggggagtcttttcattttgctcacagatggaaaggggagaccctccgagaaagtata 250
Query: 357 aagcgacatgagcattttcttgccctgcaacttggtttgaaaccaggaatgaaggtttta 416
||||| ||||| ||||||||||| || || ||| |||| || || | ||||||||| ||
Sbjct: 251 aagcgccatgaacattttcttgctcttcagctttgtttaaagcctgcaatgaaggtattg 310
Query: 417 gatgtgggctgtggaataggtggaccactgagagaaattgc 457
||||| || ||||| || || || |||||||||||||||||
Sbjct: 311 gatgttggatgtgggattggaggtccactgagagaaattgc 351
Score = 54.0 bits (27), Expect = 7e-006
Identities = 33/35 (94%)
Strand = Plus / Plus
Query: 555 tgtgattttgtcaaggcggacttcatgaagatgcc 589
|||||||||||||||| |||||||||||||||||
Sbjct: 449 tgtgattttgtcaagggagacttcatgaagatgcc 483
>dbj|BD273093.1| Materials and methods for the modification of isoprenoid content,
compostition and metabolism
Length = 582
Score = 129 bits (65), Expect = 1e-028
Identities = 182/221 (82%)
Strand = Plus / Plus
Query: 237 aactatactgatatggttaataaatactatgatcttgccactagcttctatgagtatggt 296
|||||||| || ||||| ||||||||||||||||| || ||||| ||||||||||||||
Sbjct: 301 aactatacagacatggtaaataaatactatgatctggcgactagtttctatgagtatgga 360
Query: 297 tggggtgaatccttccactttgctcacagatggaatggagaatccttacgtgaaagcatc 356
||||| || || || || ||||||||||||||||| || || || | || ||||| ||
Sbjct: 361 tggggggagtcttttcattttgctcacagatggaaaggggagaccctccgagaaagtata 420
Query: 357 aagcgacatgagcattttcttgccctgcaacttggtttgaaaccaggaatgaaggtttta 416
||||| ||||| ||||||||||| || || ||| |||| || || | ||||||||| ||
Sbjct: 421 aagcgccatgaacattttcttgctcttcacctttgtttaaagcctgcaatgaaggtattg 480
Query: 417 gatgtgggctgtggaataggtggaccactgagagaaattgc 457
||||| || ||||| || || || |||||||||||||||||
Sbjct: 481 gatgttggatgtgggattggaggtccactgagagaaattgc 521
>gb|CX713935.1|CX713935 RTPQ1_15_D05.b1_A032 Roots treated with paraquat Pinus taeda cDNA
clone RTPQ1_15_D05_A032 3', mRNA sequence
Length = 800
Score = 121 bits (61), Expect = 4e-026
Identities = 181/221 (81%)
Strand = Plus / Plus
Query: 237 aactatactgatatggttaataaatactatgatcttgccactagcttctatgagtatggt 296
|||||||| || ||||| ||||||||||||||||| || ||||| ||||||||||||||
Sbjct: 551 aactatacagacatggtaaataaatactatgatctggcgactagtttctatgagtatgga 610
Query: 297 tggggtgaatccttccactttgctcacagatggaatggagaatccttacgtgaaagcatc 356
||||| || || || || ||||||||||||||||| || || | | || ||||| ||
Sbjct: 611 tggggggagtcttttcattttgctcacagatggaaaggggagacgctccgagaaagtata 670
Query: 357 aagcgacatgagcattttcttgccctgcaacttggtttgaaaccaggaatgaaggtttta 416
||||| ||||| ||||||||||| || || ||| |||| || || | ||||||||| ||
Sbjct: 671 aagcgccatgaacattttcttgctcttcacctttgtttaaagcctgcaatgaaggtattg 730
Query: 417 gatgtgggctgtggaataggtggaccactgagagaaattgc 457
||||| || ||||| || || || |||||||||||||||||
Sbjct: 731 gatgttggatgtgggattggaggtccactgagagaaattgc 771
>gb|DR693591.1|DR693591 EST1083680 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAE147 3' end, mRNA sequence
Length = 733
Score = 121 bits (61), Expect = 4e-026
Identities = 181/221 (81%)
Strand = Plus / Plus
Query: 237 aactatactgatatggttaataaatactatgatcttgccactagcttctatgagtatggt 296
|||||||| || ||||| ||||||||||||||||| || ||||| ||||||||||||||
Sbjct: 64 aactatacagacatggtaaataaatactatgatctggcgactagtttctatgagtatgga 123
Query: 297 tggggtgaatccttccactttgctcacagatggaatggagaatccttacgtgaaagcatc 356
||||| || || || || ||||||||||||||||| || || | | || ||||| ||
Sbjct: 124 tggggggagtcttttcattttgctcacagatggaaaggggagacgctccgagaaagtata 183
Query: 357 aagcgacatgagcattttcttgccctgcaacttggtttgaaaccaggaatgaaggtttta 416
||||| ||||| ||||||||||| || || ||| |||| || || | ||||||||| ||
Sbjct: 184 aagcgccatgaacattttcttgctcttcacctttgtttaaagcctgcaatgaaggtattg 243
Query: 417 gatgtgggctgtggaataggtggaccactgagagaaattgc 457
||||| || ||||| || || || |||||||||||||||||
Sbjct: 244 gatgttggatgtgggattggaggtccactgagagaaattgc 284
Score = 46.1 bits (23), Expect = 0.002
Identities = 32/35 (91%)
Strand = Plus / Plus
Query: 555 tgtgattttgtcaaggcggacttcatgaagatgcc 589
||||||||||||| || |||||||||||||||||
Sbjct: 382 tgtgattttgtcatgggagacttcatgaagatgcc 416
>gb|DR686435.1|DR686435 EST1076513 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWABG04 3' end, mRNA sequence
Length = 808
Score = 113 bits (57), Expect = 9e-024
Identities = 180/221 (81%)
Strand = Plus / Plus
Query: 237 aactatactgatatggttaataaatactatgatcttgccactagcttctatgagtatggt 296
|||||||| || ||||| ||||||||||||||||| || ||||| ||||||||||||||
Sbjct: 369 aactatacagacatggtaaataaatactatgatctggcgactagtttctatgagtatgga 428
Query: 297 tggggtgaatccttccactttgctcacagatggaatggagaatccttacgtgaaagcatc 356
||||| || || || || |||||||||||||||| || || || | || ||||| ||
Sbjct: 429 tggggggagtcttttcattttgctcacagatggagaggggagaccctccgagaaagtata 488
Query: 357 aagcgacatgagcattttcttgccctgcaacttggtttgaaaccaggaatgaaggtttta 416
||||| ||||| ||||||||||| || || ||| |||| || || | ||||||||| ||
Sbjct: 489 aagcgccatgaacattttcttgctcttcagctttgtttaaagcctgcaatgaaggtattg 548
Query: 417 gatgtgggctgtggaataggtggaccactgagagaaattgc 457
||||| || ||||| || || || |||||||| ||||||||
Sbjct: 549 gatgttggatgtgggattggaggtccactgagggaaattgc 589
Score = 54.0 bits (27), Expect = 7e-006
Identities = 33/35 (94%)
Strand = Plus / Plus
Query: 555 tgtgattttgtcaaggcggacttcatgaagatgcc 589
|||||||||||||||| |||||||||||||||||
Sbjct: 687 tgtgattttgtcaagggagacttcatgaagatgcc 721
>gb|BQ698117.1|BQ698117 NXPV_064_G03_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_064_G03 5' similar to Arabidopsis
thaliana sequence At5g13710 24-sterol
C-methyltransferase see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 518
Score = 101 bits (51), Expect = 3e-020
Identities = 120/143 (83%)
Strand = Plus / Plus
Query: 237 aactatactgatatggttaataaatactatgatcttgccactagcttctatgagtatggt 296
|||||||| || ||||| ||||||||||||||||| || ||||| ||||||||||||||
Sbjct: 288 aactatacagacatggtaaataaatactatgatctggcgactagtttctatgagtatgga 347
Query: 297 tggggtgaatccttccactttgctcacagatggaatggagaatccttacgtgaaagcatc 356
||||| || || || || ||||||||||||||||| || || || | || ||||| ||
Sbjct: 348 tggggggagtcttttcattttgctcacagatggaaaggggagaccctccgagaaagtata 407
Query: 357 aagcgacatgagcattttcttgc 379
||||| ||||| |||||||||||
Sbjct: 408 aagcgccatgaacattttcttgc 430
>gb|BQ701207.1|BQ701207 NXSI_023_G09_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_023_G09 5' similar to Arabidopsis thaliana
sequence At5g13710 24-sterol C-methyltransferase see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 493
Score = 101 bits (51), Expect = 3e-020
Identities = 120/143 (83%)
Strand = Plus / Plus
Query: 237 aactatactgatatggttaataaatactatgatcttgccactagcttctatgagtatggt 296
|||||||| || ||||| ||||||||||||||||| || ||||| ||||||||||||||
Sbjct: 332 aactatacagacatggtaaataaatactatgatctggcgactagtttctatgagtatgga 391
Query: 297 tggggtgaatccttccactttgctcacagatggaatggagaatccttacgtgaaagcatc 356
||||| || || || || ||||||||||||||||| || || || | || ||||| ||
Sbjct: 392 tggggggagtcttttcattttgctcacagatggaaaggggagaccctccgagaaagtata 451
Query: 357 aagcgacatgagcattttcttgc 379
||||| ||||| |||||||||||
Sbjct: 452 aagcgccatgaacattttcttgc 474
>gb|DT633502.1|DT633502 EST1148433 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMFV57 3' end, mRNA sequence
Length = 857
Score = 99.6 bits (50), Expect = 1e-019
Identities = 158/194 (81%)
Strand = Plus / Plus
Query: 237 aactatactgatatggttaataaatactatgatcttgccactagcttctatgagtatggt 296
|||||||| || ||||| ||||||||||||||||| || ||||| ||||||||||||||
Sbjct: 377 aactatacagacatggtaaataaatactatgatctggcgactagtttctatgagtatgga 436
Query: 297 tggggtgaatccttccactttgctcacagatggaatggagaatccttacgtgaaagcatc 356
||||| || || || || |||||||||||||||| || || || | || ||||| ||
Sbjct: 437 tggggggagtcttttcattttgctcacagatggagaggggagaccctccgagaaagtata 496
Query: 357 aagcgacatgagcattttcttgccctgcaacttggtttgaaaccaggaatgaaggtttta 416
||||| ||||| ||||||||||| || || ||| |||| || || | ||||||||| ||
Sbjct: 497 aagcgccatgaacattttcttgctcttcagctttgtttaaagcctgcaatgaaggtattg 556
Query: 417 gatgtgggctgtgg 430
||||| || |||||
Sbjct: 557 gatgttggatgtgg 570
Score = 54.0 bits (27), Expect = 7e-006
Identities = 33/35 (94%)
Strand = Plus / Plus
Query: 555 tgtgattttgtcaaggcggacttcatgaagatgcc 589
|||||||||||||||| |||||||||||||||||
Sbjct: 696 tgtgattttgtcaagggagacttcatgaagatgcc 730
>gb|BQ699341.1|BQ699341 NXRV126_E04_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA
clone NXRV126_E04 5' similar to Arabidopsis thaliana
sequence At5g13710 24-sterol C-methyltransferase see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 363
Score = 73.8 bits (37), Expect = 7e-012
Identities = 58/65 (89%)
Strand = Plus / Plus
Query: 237 aactatactgatatggttaataaatactatgatcttgccactagcttctatgagtatggt 296
|||||||| || ||||| ||||||||||||||||| || ||||| ||||||||||||||
Sbjct: 277 aactatacagacatggtaaataaatactatgatctggcgactagtttctatgagtatgga 336
Query: 297 tgggg 301
|||||
Sbjct: 337 tgggg 341
>gb|BQ700491.1|BQ700491 NXRV107_D05_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA
clone NXRV107_D05 5' similar to Arabidopsis thaliana
sequence At5g13710 24-sterol C-methyltransferase see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 421
Score = 65.9 bits (33), Expect = 2e-009
Identities = 57/65 (87%)
Strand = Plus / Plus
Query: 237 aactatactgatatggttaataaatactatgatcttgccactagcttctatgagtatggt 296
|||||||| || ||||| ||||||||||||||||| || ||||| |||||||| |||||
Sbjct: 277 aactatacagacatggtaaataaatactatgatctggcgactagtttctatgactatgga 336
Query: 297 tgggg 301
|||||
Sbjct: 337 tgggg 341
>gb|CX645971.1|CX645971 COLD1_6_A08.g1_A029 Root cold Pinus taeda cDNA clone
COLD1_6_A08_A029 5', mRNA sequence
Length = 691
Score = 58.0 bits (29), Expect = 4e-007
Identities = 83/101 (82%)
Strand = Plus / Plus
Query: 357 aagcgacatgagcattttcttgccctgcaacttggtttgaaaccaggaatgaaggtttta 416
||||| ||||| ||||||||||| || || ||| |||| || || | ||||||||| ||
Sbjct: 2 aagcgccatgaacattttcttgctcttcagctttgtttaaagcctgcaatgaaggtattg 61
Query: 417 gatgtgggctgtggaataggtggaccactgagagaaattgc 457
||||| || ||||| || || || |||||||||||||||||
Sbjct: 62 gatgttggatgtgggattggaggtccactgagagaaattgc 102
Score = 54.0 bits (27), Expect = 7e-006
Identities = 33/35 (94%)
Strand = Plus / Plus
Query: 555 tgtgattttgtcaaggcggacttcatgaagatgcc 589
|||||||||||||||| |||||||||||||||||
Sbjct: 200 tgtgattttgtcaagggagacttcatgaagatgcc 234
>gb|CF672887.1|CF672887 RTCNT1_74_D06.g1_A029 Root control Pinus taeda cDNA clone
RTCNT1_74_D06_A029 5', mRNA sequence
Length = 740
Score = 44.1 bits (22), Expect = 0.007
Identities = 40/46 (86%)
Strand = Plus / Plus
Query: 281 cttctatgagtatggttggggtgaatccttccactttgctcacaga 326
|||||| || || ||||||||| |||| ||||||||||||| ||||
Sbjct: 203 cttctacgaatacggttggggtcaatctttccactttgctcccaga 248
>gb|DR080387.1|DR080387 RTFEPL1_22_G06.b1_A029 Roots plus added iron Pinus taeda cDNA clone
RTFEPL1_22_G06_A029 3', mRNA sequence
Length = 700
Score = 40.1 bits (20), Expect = 0.10
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 34 ccaccaccgccgagcgcgag 53
||||||||||||||||||||
Sbjct: 115 ccaccaccgccgagcgcgag 134
>gb|DR080460.1|DR080460 RTFEPL1_22_G06.g1_A029 Roots plus added iron Pinus taeda cDNA clone
RTFEPL1_22_G06_A029 5', mRNA sequence
Length = 662
Score = 40.1 bits (20), Expect = 0.10
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 34 ccaccaccgccgagcgcgag 53
||||||||||||||||||||
Sbjct: 320 ccaccaccgccgagcgcgag 339
>gb|DR110130.1|DR110130 RTS1_9_C04.b1_A029 Roots minus sulfur Pinus taeda cDNA clone
RTS1_9_C04_A029 3', mRNA sequence
Length = 839
Score = 40.1 bits (20), Expect = 0.10
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 34 ccaccaccgccgagcgcgag 53
||||||||||||||||||||
Sbjct: 470 ccaccaccgccgagcgcgag 451
>gb|DR110629.1|DR110629 RTS1_12_B03.b1_A029 Roots minus sulfur Pinus taeda cDNA clone
RTS1_12_B03_A029 3', mRNA sequence
Length = 840
Score = 40.1 bits (20), Expect = 0.10
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 34 ccaccaccgccgagcgcgag 53
||||||||||||||||||||
Sbjct: 471 ccaccaccgccgagcgcgag 452
>gb|DR118894.1|DR118894 RTMG1_19_G09.g1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_19_G09_A029 5', mRNA sequence
Length = 578
Score = 38.2 bits (19), Expect = 0.41
Identities = 32/35 (91%), Gaps = 1/35 (2%)
Strand = Plus / Plus
Query: 555 tgtgattttgtcaaggcggacttcatgaagatgcc 589
||||||||||||| || | ||||||||||||||||
Sbjct: 6 tgtgattttgtcagggag-acttcatgaagatgcc 39
>gb|DR168008.1|DR168008 RTPHOS1_22_C11.g1_A029 Roots minus phosphorous Pinus taeda cDNA
clone RTPHOS1_22_C11_A029 5', mRNA sequence
Length = 600
Score = 38.2 bits (19), Expect = 0.41
Identities = 25/27 (92%)
Strand = Plus / Plus
Query: 576 ttcatgaagatgccgttcgatgacaac 602
|||||||||||||| ||||| ||||||
Sbjct: 485 ttcatgaagatgcccttcgaggacaac 511
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 64,281
Number of Sequences: 355925
Number of extensions: 64281
Number of successful extensions: 18630
Number of sequences better than 0.5: 19
Number of HSP's better than 0.5 without gapping: 18
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 18588
Number of HSP's gapped (non-prelim): 34
length of query: 614
length of database: 217,277,237
effective HSP length: 19
effective length of query: 595
effective length of database: 210,514,662
effective search space: 125256223890
effective search space used: 125256223890
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)