BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2647494.2.1
(730 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ152982.1|BQ152982 NF027D07IR1F1062 Irradiated Medicago... 254 9e-066
gb|CX525240.1|CX525240 s13dNF16A09AT069_479114 Aphid-Infect... 254 9e-066
gb|CX525262.1|CX525262 s13dNF16C09AT070_479158 Aphid-Infect... 254 9e-066
gb|AW574009.1|AW574009 EST316600 GVN Medicago truncatula cD... 226 2e-057
gb|AA660332.1|AA660332 00203 MtRHE Medicago truncatula cDNA... 224 8e-057
gb|AJ504176.1|AJ504176 AJ504176 MTAMP Medicago truncatula c... 131 9e-029
gb|AL387802.1|AL387802 MtBC44G07R1 MtBC Medicago truncatula... 94 2e-017
gb|CX529542.1|CX529542 s13dNF97E03MJ019_245135 Methyl Jasmo... 76 5e-012
gb|BG582270.1|BG582270 EST484010 GVN Medicago truncatula cD... 74 2e-011
gb|AL387801.1|AL387801 MtBC44G07F1 MtBC Medicago truncatula... 50 3e-004
gb|AC158501.3| Medicago truncatula chromosome 2 BAC clone m... 40 0.25
>gb|BQ152982.1|BQ152982 NF027D07IR1F1062 Irradiated Medicago truncatula cDNA clone
NF027D07IR 5', mRNA sequence
Length = 661
Score = 254 bits (128), Expect = 9e-066
Identities = 341/412 (82%)
Strand = Plus / Minus
Query: 250 gtccactccttcgctgtctcaacagcttcagcttcgttggatttccagtgtttcgcaatg 309
|||||||| || |||||||||||||| ||||| ||||| |||||||| || || ||||||
Sbjct: 527 gtccactctttggctgtctcaacagcctcagcctcgttagatttccaatgcttagcaatg 468
Query: 310 ttgtctgagagaggatcatctgggtttggggcgctcagaagtgcctgaatgctcagtaga 369
|| || || |||||||||||||||||||| || |||| ||| || ||||| |||| ||
Sbjct: 467 ttctcagaaagaggatcatctgggtttggtgcactcaaaagagcttgaatactcaaaagt 408
Query: 370 actgtacgtatctgaagagctggactccatttgtccttaagaatgtcgaggcatatccta 429
|| || || ||||| || || |||||||| || || || ||||| || || ||||||||
Sbjct: 407 acagtgcgaatctggagggcaggactccacttatctttcagaatatcaagacatatcctg 348
Query: 430 ccaagcttgtcaatattaggatggtaaatttttgtgagaaatctaacttttggggcagcc 489
|||||||||||||| |||||||| || |||||||| | || ||||| ||||| ||||||
Sbjct: 347 ccaagcttgtcaatgttaggatgatatatttttgtcaagaacctaacctttggagcagcc 288
Query: 490 attggatattcctcaggcaagaatagttcaagcttgaaaactccaccttcatagggtgac 549
||||||||||| |||||||| |||||||| |||||||||||||| |||||||| || |||
Sbjct: 287 attggatattcttcaggcaaaaatagttctagcttgaaaactcccccttcataaggagac 228
Query: 550 tgcgcggggccaaggatcatgacattgaagtaccgcatattctcctccgacggcgacgcg 609
|| | |||||||||||||| |||||||| ||||||||||| || || || || || ||
Sbjct: 227 tgagttgggccaaggatcatcacattgaaataccgcatattgtcttcggaaggggatgca 168
Query: 610 ctaatccccggtgctggttcgctgagaagccgctgcgtctccttgatgattc 661
||||| || || ||||| || ||||| | ||||| || |||||||||||||
Sbjct: 167 ctaattccaggcgctggctcactgagcaaacgctgtgtttccttgatgattc 116
>gb|CX525240.1|CX525240 s13dNF16A09AT069_479114 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 549
Score = 254 bits (128), Expect = 9e-066
Identities = 341/412 (82%)
Strand = Plus / Minus
Query: 250 gtccactccttcgctgtctcaacagcttcagcttcgttggatttccagtgtttcgcaatg 309
|||||||| || |||||||||||||| ||||| ||||| |||||||| || || ||||||
Sbjct: 523 gtccactctttggctgtctcaacagcctcagcctcgttagatttccaatgcttagcaatg 464
Query: 310 ttgtctgagagaggatcatctgggtttggggcgctcagaagtgcctgaatgctcagtaga 369
|| || || |||||||||||||||||||| || |||| ||| || ||||| |||| ||
Sbjct: 463 ttctcagaaagaggatcatctgggtttggtgcactcaaaagagcttgaatactcaaaagt 404
Query: 370 actgtacgtatctgaagagctggactccatttgtccttaagaatgtcgaggcatatccta 429
|| || || ||||| || || |||||||| || || || ||||| || || ||||||||
Sbjct: 403 acagtgcgaatctggagggcaggactccacttatctttcagaatatcaagacatatcctg 344
Query: 430 ccaagcttgtcaatattaggatggtaaatttttgtgagaaatctaacttttggggcagcc 489
|||||||||||||| |||||||| || |||||||| | || ||||| ||||| ||||||
Sbjct: 343 ccaagcttgtcaatgttaggatgatatatttttgtcaagaacctaacctttggagcagcc 284
Query: 490 attggatattcctcaggcaagaatagttcaagcttgaaaactccaccttcatagggtgac 549
||||||||||| |||||||| |||||||| |||||||||||||| |||||||| || |||
Sbjct: 283 attggatattcttcaggcaaaaatagttctagcttgaaaactcccccttcataaggagac 224
Query: 550 tgcgcggggccaaggatcatgacattgaagtaccgcatattctcctccgacggcgacgcg 609
|| | |||||||||||||| |||||||| ||||||||||| || || || || || ||
Sbjct: 223 tgagttgggccaaggatcatcacattgaaataccgcatattgtcttcggaaggggatgca 164
Query: 610 ctaatccccggtgctggttcgctgagaagccgctgcgtctccttgatgattc 661
||||| || || ||||| || ||||| | ||||| || |||||||||||||
Sbjct: 163 ctaattccaggcgctggctcactgagcaaacgctgtgtttccttgatgattc 112
>gb|CX525262.1|CX525262 s13dNF16C09AT070_479158 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 549
Score = 254 bits (128), Expect = 9e-066
Identities = 341/412 (82%)
Strand = Plus / Minus
Query: 250 gtccactccttcgctgtctcaacagcttcagcttcgttggatttccagtgtttcgcaatg 309
|||||||| || |||||||||||||| ||||| ||||| |||||||| || || ||||||
Sbjct: 523 gtccactctttggctgtctcaacagcctcagcctcgttagatttccaatgcttagcaatg 464
Query: 310 ttgtctgagagaggatcatctgggtttggggcgctcagaagtgcctgaatgctcagtaga 369
|| || || |||||||||||||||||||| || |||| ||| || ||||| |||| ||
Sbjct: 463 ttctcagaaagaggatcatctgggtttggtgcactcaaaagagcttgaatactcaaaagt 404
Query: 370 actgtacgtatctgaagagctggactccatttgtccttaagaatgtcgaggcatatccta 429
|| || || ||||| || || |||||||| || || || ||||| || || ||||||||
Sbjct: 403 acagtgcgaatctggagggcaggactccacttatctttcagaatatcaagacatatcctg 344
Query: 430 ccaagcttgtcaatattaggatggtaaatttttgtgagaaatctaacttttggggcagcc 489
|||||||||||||| |||||||| || |||||||| | || ||||| ||||| ||||||
Sbjct: 343 ccaagcttgtcaatgttaggatgatatatttttgtcaagaacctaacctttggagcagcc 284
Query: 490 attggatattcctcaggcaagaatagttcaagcttgaaaactccaccttcatagggtgac 549
||||||||||| |||||||| |||||||| |||||||||||||| |||||||| || |||
Sbjct: 283 attggatattcttcaggcaaaaatagttctagcttgaaaactcccccttcataaggagac 224
Query: 550 tgcgcggggccaaggatcatgacattgaagtaccgcatattctcctccgacggcgacgcg 609
|| | |||||||||||||| |||||||| ||||||||||| || || || || || ||
Sbjct: 223 tgagttgggccaaggatcatcacattgaaataccgcatattgtcttcggaaggggatgca 164
Query: 610 ctaatccccggtgctggttcgctgagaagccgctgcgtctccttgatgattc 661
||||| || || ||||| || ||||| | ||||| || |||||||||||||
Sbjct: 163 ctaattccaggcgctggctcactgagcaaacgctgtgtttccttgatgattc 112
>gb|AW574009.1|AW574009 EST316600 GVN Medicago truncatula cDNA clone pGVN-50H8, mRNA
sequence
Length = 515
Score = 226 bits (114), Expect = 2e-057
Identities = 282/338 (83%)
Strand = Plus / Minus
Query: 262 gctgtctcaacagcttcagcttcgttggatttccagtgtttcgcaatgttgtctgagaga 321
|||||||||||||| ||||| || || |||||||| || || |||||||| || || |||
Sbjct: 339 gctgtctcaacagcctcagcctcattagatttccaatgcttagcaatgttctcagaaaga 280
Query: 322 ggatcatctgggtttggggcgctcagaagtgcctgaatgctcagtagaactgtacgtatc 381
||||||||||||||||| || |||| ||| || ||||| |||| || || || || |||
Sbjct: 279 ggatcatctgggtttggtgcactcaaaagagcttgaatactcaaaagtacagtgcgaatc 220
Query: 382 tgaagagctggactccatttgtccttaagaatgtcgaggcatatcctaccaagcttgtca 441
|| || || ||| |||| || || || ||||| || || |||||||| ||||||||||||
Sbjct: 219 tggagggcaggagtccacttatctttcagaatatcaagacatatcctgccaagcttgtca 160
Query: 442 atattaggatggtaaatttttgtgagaaatctaacttttggggcagccattggatattcc 501
|| |||||||| || |||||||| | || ||||| ||||| |||||||||||||||||
Sbjct: 159 atgttaggatgatatatttttgtcaagaacctaacctttggagcagccattggatattct 100
Query: 502 tcaggcaagaatagttcaagcttgaaaactccaccttcatagggtgactgcgcggggcca 561
|||||||| |||||||| |||||||||||||| |||||||| || ||||| | ||||||
Sbjct: 99 tcaggcaaaaatagttctagcttgaaaactcccccttcataaggagactgagttgggcca 40
Query: 562 aggatcatgacattgaagtaccgcatattctcctccga 599
|||||||| |||||||| ||||||||||| || |||||
Sbjct: 39 aggatcatcacattgaaataccgcatattgtcttccga 2
>gb|AA660332.1|AA660332 00203 MtRHE Medicago truncatula cDNA 5' similar to ubiquitin
conjugating enzyme E2, mRNA sequence
Length = 541
Score = 224 bits (113), Expect = 8e-057
Identities = 340/414 (82%), Gaps = 2/414 (0%)
Strand = Plus / Minus
Query: 250 gtccactccttcgctgtctcaacagcttcagcttcgttggatttccagtgtttcgcaatg 309
||||| || || ||||| |||||||||||||||||||| |||||||| || || ||||||
Sbjct: 515 gtccantctttggctgtntcaacagcttcagcttcgttagatttccaatgcttagcaatg 456
Query: 310 ttgtctgagagaggatcatctgggtttggggcgct-cagaagtgcctgaatgctcagtag 368
|| || || |||||||||||||||||||| || | || ||| || ||||| |||| ||
Sbjct: 455 ttctcagaaagaggatcatctgggtttggtgcanttcaaaagagcttgaatactcaaaag 396
Query: 369 aactgtacgtatctgaagagctggactccat-ttgtccttaagaatgtcgaggcatatcc 427
|| || || ||||| || || |||||||| || || || ||||| || || |||||||
Sbjct: 395 tacagtgcgaatctggagggcaggactccaacttatctttcagaatatcaagacatatcc 336
Query: 428 taccaagcttgtcaatattaggatggtaaatttttgtgagaaatctaacttttggggcag 487
| |||||||||||||| |||||||| || |||||||| | || ||||| ||||| ||||
Sbjct: 335 tgccaagcttgtcaatgttaggatgatatatttttgtcaagaacctaacctttggagcag 276
Query: 488 ccattggatattcctcaggcaagaatagttcaagcttgaaaactccaccttcatagggtg 547
||||||||||||| |||||||| |||||||| |||||||||||||| |||||||| || |
Sbjct: 275 ccattggatattcttcaggcaaaaatagttctagcttgaaaactcccccttcataaggag 216
Query: 548 actgcgcggggccaaggatcatgacattgaagtaccgcatattctcctccgacggcgacg 607
|||| | |||||||||||||| |||||||| ||||||||||| || || || || || |
Sbjct: 215 actgagttgggccaaggatcatcacattgaaataccgcatattgtcttcggaaggggatg 156
Query: 608 cgctaatccccggtgctggttcgctgagaagccgctgcgtctccttgatgattc 661
| ||||| || || ||||| || ||||| | ||||| || |||||||||||||
Sbjct: 155 cactaattccaggcgctggctcactgagcaaacgctgtgtttccttgatgattc 102
>gb|AJ504176.1|AJ504176 AJ504176 MTAMP Medicago truncatula cDNA clone mtgmadc120044b06,
mRNA sequence
Length = 280
Score = 131 bits (66), Expect = 9e-029
Identities = 159/190 (83%)
Strand = Plus / Minus
Query: 472 ctaacttttggggcagccattggatattcctcaggcaagaatagttcaagcttgaaaact 531
||||| ||||| ||||||||||||||||| |||||||| ||||||| ||||||||||||
Sbjct: 270 ctaacctttggagcagccattggatattcttcaggcaaatatagttctagcttgaaaact 211
Query: 532 ccaccttcatagggtgactgcgcggggccaaggatcatgacattgaagtaccgcatattc 591
|| |||||||| || ||||| | |||||||||||||| |||||||| |||||||||||
Sbjct: 210 cccccttcataaggagactgagttgggccaaggatcatcacattgaaataccgcatattg 151
Query: 592 tcctccgacggcgacgcgctaatccccggtgctggttcgctgagaagccgctgcgtctcc 651
|| || || || || || ||||| || || ||||| || ||||| | ||||| || |||
Sbjct: 150 tcttcggaaggggatgcactaattccaggcgctggctcactgagcaaacgctgtgtttcc 91
Query: 652 ttgatgattc 661
||||||||||
Sbjct: 90 ttgatgattc 81
>gb|AL387802.1|AL387802 MtBC44G07R1 MtBC Medicago truncatula cDNA clone MtBC44G07 T7, mRNA
sequence
Length = 539
Score = 93.7 bits (47), Expect = 2e-017
Identities = 98/115 (85%)
Strand = Plus / Minus
Query: 250 gtccactccttcgctgtctcaacagcttcagcttcgttggatttccagtgtttcgcaatg 309
|||||||| || |||||||||||||| ||||| ||||| |||||||| || || ||||||
Sbjct: 183 gtccactctttggctgtctcaacagcctcagcctcgttagatttccaatgcttagcaatg 124
Query: 310 ttgtctgagagaggatcatctgggtttggggcgctcagaagtgcctgaatgctca 364
|| || || |||||||||||||||||||| || |||| ||| || ||||| ||||
Sbjct: 123 ttctcagaaagaggatcatctgggtttggtgcactcaaaagagcttgaatactca 69
>gb|CX529542.1|CX529542 s13dNF97E03MJ019_245135 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 553
Score = 75.8 bits (38), Expect = 5e-012
Identities = 83/98 (84%)
Strand = Plus / Minus
Query: 262 gctgtctcaacagcttcagcttcgttggatttccagtgtttcgcaatgttgtctgagaga 321
|||||||||||||| ||||| || || |||||||| || || |||||||| || || |||
Sbjct: 131 gctgtctcaacagcctcagcctcattagatttccaatgcttagcaatgttctcagaaaga 72
Query: 322 ggatcatctgggtttggggcgctcagaagtgcctgaat 359
||||||||||||||||| || |||| ||| || |||||
Sbjct: 71 ggatcatctgggtttggtgcactcaaaagagcttgaat 34
>gb|BG582270.1|BG582270 EST484010 GVN Medicago truncatula cDNA clone pGVN-69M23 5' end,
mRNA sequence
Length = 688
Score = 73.8 bits (37), Expect = 2e-011
Identities = 82/97 (84%)
Strand = Plus / Minus
Query: 263 ctgtctcaacagcttcagcttcgttggatttccagtgtttcgcaatgttgtctgagagag 322
||||||||||||| ||||| || || |||||||| || || |||||||| || || ||||
Sbjct: 114 ctgtctcaacagcctcagcctcattagatttccaatgcttagcaatgttctcagaaagag 55
Query: 323 gatcatctgggtttggggcgctcagaagtgcctgaat 359
|||||||||||||||| || |||| ||| || |||||
Sbjct: 54 gatcatctgggtttggtgcactcaaaagagcttgaat 18
>gb|AL387801.1|AL387801 MtBC44G07F1 MtBC Medicago truncatula cDNA clone MtBC44G07 T3, mRNA
sequence
Length = 479
Score = 50.1 bits (25), Expect = 3e-004
Identities = 52/61 (85%)
Strand = Plus / Minus
Query: 391 ggactccatttgtccttaagaatgtcgaggcatatcctaccaagcttgtcaatattagga 450
|||||||| || || || ||||| || || |||||||| |||||||||||||| ||||||
Sbjct: 426 ggactccacttatctttcagaatatcaagacatatcctgccaagcttgtcaatgttagga 367
Query: 451 t 451
|
Sbjct: 366 t 366
>gb|AC158501.3| Medicago truncatula chromosome 2 BAC clone mth2-70b22, complete
sequence
Length = 108930
Score = 40.1 bits (20), Expect = 0.25
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 125 cattgcaacatttcacaaat 144
||||||||||||||||||||
Sbjct: 79791 cattgcaacatttcacaaat 79810
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 145,199
Number of Sequences: 392609
Number of extensions: 145199
Number of successful extensions: 11988
Number of sequences better than 0.5: 11
Number of HSP's better than 0.5 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 11967
Number of HSP's gapped (non-prelim): 20
length of query: 730
length of database: 441,732,993
effective HSP length: 19
effective length of query: 711
effective length of database: 434,273,422
effective search space: 308768403042
effective search space used: 308768403042
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)