BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716286.2.1
(1104 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ152982.1|BQ152982 NF027D07IR1F1062 Irradiated Medicago... 234 1e-059
gb|CX525240.1|CX525240 s13dNF16A09AT069_479114 Aphid-Infect... 234 1e-059
gb|CX525262.1|CX525262 s13dNF16C09AT070_479158 Aphid-Infect... 234 1e-059
gb|AW574009.1|AW574009 EST316600 GVN Medicago truncatula cD... 210 2e-052
gb|AA660332.1|AA660332 00203 MtRHE Medicago truncatula cDNA... 196 3e-048
gb|AL387802.1|AL387802 MtBC44G07R1 MtBC Medicago truncatula... 101 1e-019
gb|AJ504176.1|AJ504176 AJ504176 MTAMP Medicago truncatula c... 101 1e-019
gb|CX529542.1|CX529542 s13dNF97E03MJ019_245135 Methyl Jasmo... 82 1e-013
gb|BG582270.1|BG582270 EST484010 GVN Medicago truncatula cD... 72 1e-010
gb|AL387801.1|AL387801 MtBC44G07F1 MtBC Medicago truncatula... 68 2e-009
gb|CG956422.1|CG956422 MBEIN95TF mth2 Medicago truncatula g... 40 0.39
gb|CG971152.1|CG971152 MBEBM38TF mth2 Medicago truncatula g... 40 0.39
gb|AC119418.5| Medicago truncatula clone mth1-23l16, comple... 40 0.39
gb|AC153120.10| Medicago truncatula clone mth2-20n3, WORKIN... 40 0.39
>gb|BQ152982.1|BQ152982 NF027D07IR1F1062 Irradiated Medicago truncatula cDNA clone
NF027D07IR 5', mRNA sequence
Length = 661
Score = 234 bits (118), Expect = 1e-059
Identities = 337/410 (82%)
Strand = Plus / Minus
Query: 531 gtccactccttggctgtttcaacagcttctacctcattggatttccagtgctttgcaatg 590
|||||||| |||||||| |||||||| || |||| || |||||||| ||||| ||||||
Sbjct: 527 gtccactctttggctgtctcaacagcctcagcctcgttagatttccaatgcttagcaatg 468
Query: 591 ttatcggagagagggtcatctggatttggtgcactcagtagagcctgtatactcaaaaga 650
|| || || ||||| |||||||| ||||||||||||| ||||| || |||||||||||
Sbjct: 467 ttctcagaaagaggatcatctgggtttggtgcactcaaaagagcttgaatactcaaaagt 408
Query: 651 actgttcgaatctgaagtgctgggctccatttgtccttgagaatgtcgaggcatatccta 710
|| || |||||||| || || || ||||| || || || ||||| || || ||||||||
Sbjct: 407 acagtgcgaatctggagggcaggactccacttatctttcagaatatcaagacatatcctg 348
Query: 711 ccaagcttgtcaatgttgggatgataaatcttggtcagaaacctaacctttggggcagcc 770
||||||||||||||||| |||||||| || || |||| |||||||||||||| ||||||
Sbjct: 347 ccaagcttgtcaatgttaggatgatatatttttgtcaagaacctaacctttggagcagcc 288
Query: 771 atgggatattcctcaggtaaaaagagttcaagcttaaaaactccaccttcatagggcgac 830
|| |||||||| ||||| ||||| ||||| ||||| |||||||| |||||||| || |||
Sbjct: 287 attggatattcttcaggcaaaaatagttctagcttgaaaactcccccttcataaggagac 228
Query: 831 tgcgccggcccaaggatcataacgttgaagtagcgcatgttctcctccgagggcgacgcg 890
|| | || ||||||||||| || ||||| || ||||| || || || || || || ||
Sbjct: 227 tgagttgggccaaggatcatcacattgaaataccgcatattgtcttcggaaggggatgca 168
Query: 891 ctgatccccggcgctggctcgctgagcagtcgctgcgtctccttgatgat 940
|| || || ||||||||||| ||||||| ||||| || |||||||||||
Sbjct: 167 ctaattccaggcgctggctcactgagcaaacgctgtgtttccttgatgat 118
>gb|CX525240.1|CX525240 s13dNF16A09AT069_479114 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 549
Score = 234 bits (118), Expect = 1e-059
Identities = 337/410 (82%)
Strand = Plus / Minus
Query: 531 gtccactccttggctgtttcaacagcttctacctcattggatttccagtgctttgcaatg 590
|||||||| |||||||| |||||||| || |||| || |||||||| ||||| ||||||
Sbjct: 523 gtccactctttggctgtctcaacagcctcagcctcgttagatttccaatgcttagcaatg 464
Query: 591 ttatcggagagagggtcatctggatttggtgcactcagtagagcctgtatactcaaaaga 650
|| || || ||||| |||||||| ||||||||||||| ||||| || |||||||||||
Sbjct: 463 ttctcagaaagaggatcatctgggtttggtgcactcaaaagagcttgaatactcaaaagt 404
Query: 651 actgttcgaatctgaagtgctgggctccatttgtccttgagaatgtcgaggcatatccta 710
|| || |||||||| || || || ||||| || || || ||||| || || ||||||||
Sbjct: 403 acagtgcgaatctggagggcaggactccacttatctttcagaatatcaagacatatcctg 344
Query: 711 ccaagcttgtcaatgttgggatgataaatcttggtcagaaacctaacctttggggcagcc 770
||||||||||||||||| |||||||| || || |||| |||||||||||||| ||||||
Sbjct: 343 ccaagcttgtcaatgttaggatgatatatttttgtcaagaacctaacctttggagcagcc 284
Query: 771 atgggatattcctcaggtaaaaagagttcaagcttaaaaactccaccttcatagggcgac 830
|| |||||||| ||||| ||||| ||||| ||||| |||||||| |||||||| || |||
Sbjct: 283 attggatattcttcaggcaaaaatagttctagcttgaaaactcccccttcataaggagac 224
Query: 831 tgcgccggcccaaggatcataacgttgaagtagcgcatgttctcctccgagggcgacgcg 890
|| | || ||||||||||| || ||||| || ||||| || || || || || || ||
Sbjct: 223 tgagttgggccaaggatcatcacattgaaataccgcatattgtcttcggaaggggatgca 164
Query: 891 ctgatccccggcgctggctcgctgagcagtcgctgcgtctccttgatgat 940
|| || || ||||||||||| ||||||| ||||| || |||||||||||
Sbjct: 163 ctaattccaggcgctggctcactgagcaaacgctgtgtttccttgatgat 114
>gb|CX525262.1|CX525262 s13dNF16C09AT070_479158 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 549
Score = 234 bits (118), Expect = 1e-059
Identities = 337/410 (82%)
Strand = Plus / Minus
Query: 531 gtccactccttggctgtttcaacagcttctacctcattggatttccagtgctttgcaatg 590
|||||||| |||||||| |||||||| || |||| || |||||||| ||||| ||||||
Sbjct: 523 gtccactctttggctgtctcaacagcctcagcctcgttagatttccaatgcttagcaatg 464
Query: 591 ttatcggagagagggtcatctggatttggtgcactcagtagagcctgtatactcaaaaga 650
|| || || ||||| |||||||| ||||||||||||| ||||| || |||||||||||
Sbjct: 463 ttctcagaaagaggatcatctgggtttggtgcactcaaaagagcttgaatactcaaaagt 404
Query: 651 actgttcgaatctgaagtgctgggctccatttgtccttgagaatgtcgaggcatatccta 710
|| || |||||||| || || || ||||| || || || ||||| || || ||||||||
Sbjct: 403 acagtgcgaatctggagggcaggactccacttatctttcagaatatcaagacatatcctg 344
Query: 711 ccaagcttgtcaatgttgggatgataaatcttggtcagaaacctaacctttggggcagcc 770
||||||||||||||||| |||||||| || || |||| |||||||||||||| ||||||
Sbjct: 343 ccaagcttgtcaatgttaggatgatatatttttgtcaagaacctaacctttggagcagcc 284
Query: 771 atgggatattcctcaggtaaaaagagttcaagcttaaaaactccaccttcatagggcgac 830
|| |||||||| ||||| ||||| ||||| ||||| |||||||| |||||||| || |||
Sbjct: 283 attggatattcttcaggcaaaaatagttctagcttgaaaactcccccttcataaggagac 224
Query: 831 tgcgccggcccaaggatcataacgttgaagtagcgcatgttctcctccgagggcgacgcg 890
|| | || ||||||||||| || ||||| || ||||| || || || || || || ||
Sbjct: 223 tgagttgggccaaggatcatcacattgaaataccgcatattgtcttcggaaggggatgca 164
Query: 891 ctgatccccggcgctggctcgctgagcagtcgctgcgtctccttgatgat 940
|| || || ||||||||||| ||||||| ||||| || |||||||||||
Sbjct: 163 ctaattccaggcgctggctcactgagcaaacgctgtgtttccttgatgat 114
>gb|AW574009.1|AW574009 EST316600 GVN Medicago truncatula cDNA clone pGVN-50H8, mRNA
sequence
Length = 515
Score = 210 bits (106), Expect = 2e-052
Identities = 280/338 (82%)
Strand = Plus / Minus
Query: 531 gtccactccttggctgtttcaacagcttctacctcattggatttccagtgctttgcaatg 590
||||| || |||||||| |||||||| || ||||||| |||||||| ||||| ||||||
Sbjct: 351 gtccaatctttggctgtctcaacagcctcagcctcattagatttccaatgcttagcaatg 292
Query: 591 ttatcggagagagggtcatctggatttggtgcactcagtagagcctgtatactcaaaaga 650
|| || || ||||| |||||||| ||||||||||||| ||||| || |||||||||||
Sbjct: 291 ttctcagaaagaggatcatctgggtttggtgcactcaaaagagcttgaatactcaaaagt 232
Query: 651 actgttcgaatctgaagtgctgggctccatttgtccttgagaatgtcgaggcatatccta 710
|| || |||||||| || || || |||| || || || ||||| || || ||||||||
Sbjct: 231 acagtgcgaatctggagggcaggagtccacttatctttcagaatatcaagacatatcctg 172
Query: 711 ccaagcttgtcaatgttgggatgataaatcttggtcagaaacctaacctttggggcagcc 770
||||||||||||||||| |||||||| || || |||| |||||||||||||| ||||||
Sbjct: 171 ccaagcttgtcaatgttaggatgatatatttttgtcaagaacctaacctttggagcagcc 112
Query: 771 atgggatattcctcaggtaaaaagagttcaagcttaaaaactccaccttcatagggcgac 830
|| |||||||| ||||| ||||| ||||| ||||| |||||||| |||||||| || |||
Sbjct: 111 attggatattcttcaggcaaaaatagttctagcttgaaaactcccccttcataaggagac 52
Query: 831 tgcgccggcccaaggatcataacgttgaagtagcgcat 868
|| | || ||||||||||| || ||||| || |||||
Sbjct: 51 tgagttgggccaaggatcatcacattgaaataccgcat 14
>gb|AA660332.1|AA660332 00203 MtRHE Medicago truncatula cDNA 5' similar to ubiquitin
conjugating enzyme E2, mRNA sequence
Length = 541
Score = 196 bits (99), Expect = 3e-048
Identities = 335/412 (81%), Gaps = 2/412 (0%)
Strand = Plus / Minus
Query: 531 gtccactccttggctgtttcaacagcttctacctcattggatttccagtgctttgcaatg 590
||||| || |||||||| ||||||||||| | || || |||||||| ||||| ||||||
Sbjct: 515 gtccantctttggctgtntcaacagcttcagcttcgttagatttccaatgcttagcaatg 456
Query: 591 ttatcggagagagggtcatctggatttggtgcact-cagtagagcctgtatactcaaaag 649
|| || || ||||| |||||||| ||||||||| | || ||||| || |||||||||||
Sbjct: 455 ttctcagaaagaggatcatctgggtttggtgcanttcaaaagagcttgaatactcaaaag 396
Query: 650 aactgttcgaatctgaagtgctgggctccat-ttgtccttgagaatgtcgaggcatatcc 708
|| || |||||||| || || || ||||| || || || ||||| || || |||||||
Sbjct: 395 tacagtgcgaatctggagggcaggactccaacttatctttcagaatatcaagacatatcc 336
Query: 709 taccaagcttgtcaatgttgggatgataaatcttggtcagaaacctaacctttggggcag 768
| ||||||||||||||||| |||||||| || || |||| |||||||||||||| ||||
Sbjct: 335 tgccaagcttgtcaatgttaggatgatatatttttgtcaagaacctaacctttggagcag 276
Query: 769 ccatgggatattcctcaggtaaaaagagttcaagcttaaaaactccaccttcatagggcg 828
|||| |||||||| ||||| ||||| ||||| ||||| |||||||| |||||||| || |
Sbjct: 275 ccattggatattcttcaggcaaaaatagttctagcttgaaaactcccccttcataaggag 216
Query: 829 actgcgccggcccaaggatcataacgttgaagtagcgcatgttctcctccgagggcgacg 888
|||| | || ||||||||||| || ||||| || ||||| || || || || || || |
Sbjct: 215 actgagttgggccaaggatcatcacattgaaataccgcatattgtcttcggaaggggatg 156
Query: 889 cgctgatccccggcgctggctcgctgagcagtcgctgcgtctccttgatgat 940
| || || || ||||||||||| ||||||| ||||| || |||||||||||
Sbjct: 155 cactaattccaggcgctggctcactgagcaaacgctgtgtttccttgatgat 104
>gb|AL387802.1|AL387802 MtBC44G07R1 MtBC Medicago truncatula cDNA clone MtBC44G07 T7, mRNA
sequence
Length = 539
Score = 101 bits (51), Expect = 1e-019
Identities = 147/179 (82%)
Strand = Plus / Minus
Query: 531 gtccactccttggctgtttcaacagcttctacctcattggatttccagtgctttgcaatg 590
|||||||| |||||||| |||||||| || |||| || |||||||| ||||| ||||||
Sbjct: 183 gtccactctttggctgtctcaacagcctcagcctcgttagatttccaatgcttagcaatg 124
Query: 591 ttatcggagagagggtcatctggatttggtgcactcagtagagcctgtatactcaaaaga 650
|| || || ||||| |||||||| ||||||||||||| ||||| || |||||||||||
Sbjct: 123 ttctcagaaagaggatcatctgggtttggtgcactcaaaagagcttgaatactcaaaagt 64
Query: 651 actgttcgaatctgaagtgctgggctccatttgtccttgagaatgtcgaggcatatcct 709
|| || |||||||| || || || ||||| || || || ||||| || || ||||||||
Sbjct: 63 acagtgcgaatctggagggcaggactccacttatctttcagaatatcaagacatatcct 5
>gb|AJ504176.1|AJ504176 AJ504176 MTAMP Medicago truncatula cDNA clone mtgmadc120044b06,
mRNA sequence
Length = 280
Score = 101 bits (51), Expect = 1e-019
Identities = 156/191 (81%)
Strand = Plus / Minus
Query: 750 aacctaacctttggggcagccatgggatattcctcaggtaaaaagagttcaagcttaaaa 809
|||||||||||||| |||||||| |||||||| ||||| ||| | ||||| ||||| |||
Sbjct: 273 aacctaacctttggagcagccattggatattcttcaggcaaatatagttctagcttgaaa 214
Query: 810 actccaccttcatagggcgactgcgccggcccaaggatcataacgttgaagtagcgcatg 869
||||| |||||||| || ||||| | || ||||||||||| || ||||| || |||||
Sbjct: 213 actcccccttcataaggagactgagttgggccaaggatcatcacattgaaataccgcata 154
Query: 870 ttctcctccgagggcgacgcgctgatccccggcgctggctcgctgagcagtcgctgcgtc 929
|| || || || || || || || || || ||||||||||| ||||||| ||||| ||
Sbjct: 153 ttgtcttcggaaggggatgcactaattccaggcgctggctcactgagcaaacgctgtgtt 94
Query: 930 tccttgatgat 940
|||||||||||
Sbjct: 93 tccttgatgat 83
>gb|CX529542.1|CX529542 s13dNF97E03MJ019_245135 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 553
Score = 81.8 bits (41), Expect = 1e-013
Identities = 83/97 (85%)
Strand = Plus / Minus
Query: 531 gtccactccttggctgtttcaacagcttctacctcattggatttccagtgctttgcaatg 590
||||| || |||||||| |||||||| || ||||||| |||||||| ||||| ||||||
Sbjct: 143 gtccaatctttggctgtctcaacagcctcagcctcattagatttccaatgcttagcaatg 84
Query: 591 ttatcggagagagggtcatctggatttggtgcactca 627
|| || || ||||| |||||||| |||||||||||||
Sbjct: 83 ttctcagaaagaggatcatctgggtttggtgcactca 47
>gb|BG582270.1|BG582270 EST484010 GVN Medicago truncatula cDNA clone pGVN-69M23 5' end,
mRNA sequence
Length = 688
Score = 71.9 bits (36), Expect = 1e-010
Identities = 72/84 (85%)
Strand = Plus / Minus
Query: 544 ctgtttcaacagcttctacctcattggatttccagtgctttgcaatgttatcggagagag 603
|||| |||||||| || ||||||| |||||||| ||||| |||||||| || || ||||
Sbjct: 114 ctgtctcaacagcctcagcctcattagatttccaatgcttagcaatgttctcagaaagag 55
Query: 604 ggtcatctggatttggtgcactca 627
| |||||||| |||||||||||||
Sbjct: 54 gatcatctgggtttggtgcactca 31
>gb|AL387801.1|AL387801 MtBC44G07F1 MtBC Medicago truncatula cDNA clone MtBC44G07 T3, mRNA
sequence
Length = 479
Score = 67.9 bits (34), Expect = 2e-009
Identities = 94/114 (82%)
Strand = Plus / Minus
Query: 619 gtgcactcagtagagcctgtatactcaaaagaactgttcgaatctgaagtgctgggctcc 678
||||||||| ||||| || ||||||||||| || || |||||||| || || || ||||
Sbjct: 479 gtgcactcaaaagagcttgaatactcaaaagtacagtgcgaatctggagggcaggactcc 420
Query: 679 atttgtccttgagaatgtcgaggcatatcctaccaagcttgtcaatgttgggat 732
| || || || ||||| || || |||||||| ||||||||||||||||| ||||
Sbjct: 419 acttatctttcagaatatcaagacatatcctgccaagcttgtcaatgttaggat 366
Score = 42.1 bits (21), Expect = 0.098
Identities = 36/41 (87%)
Strand = Plus / Minus
Query: 900 ggcgctggctcgctgagcagtcgctgcgtctccttgatgat 940
||||||||||| ||||||| ||||| || |||||||||||
Sbjct: 149 ggcgctggctcactgagcaaacgctgtgtttccttgatgat 109
>gb|CG956422.1|CG956422 MBEIN95TF mth2 Medicago truncatula genomic clone 63O22, DNA
sequence
Length = 853
Score = 40.1 bits (20), Expect = 0.39
Identities = 38/44 (86%)
Strand = Plus / Minus
Query: 716 cttgtcaatgttgggatgataaatcttggtcagaaacctaacct 759
|||||||||||| |||||||| || || |||| ||||||||||
Sbjct: 548 cttgtcaatgttaggatgatatatttttgtcaagaacctaacct 505
>gb|CG971152.1|CG971152 MBEBM38TF mth2 Medicago truncatula genomic clone 20G3, DNA sequence
Length = 760
Score = 40.1 bits (20), Expect = 0.39
Identities = 38/44 (86%)
Strand = Plus / Minus
Query: 716 cttgtcaatgttgggatgataaatcttggtcagaaacctaacct 759
|||||||||||| |||||||| || || |||| ||||||||||
Sbjct: 501 cttgtcaatgttaggatgatatatttttgtcaagaacctaacct 458
>gb|AC119418.5| Medicago truncatula clone mth1-23l16, complete sequence
Length = 112695
Score = 40.1 bits (20), Expect = 0.39
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 124 aaaacatgaccaggtgcaactcga 147
|||||||||| |||||||||||||
Sbjct: 8956 aaaacatgacaaggtgcaactcga 8979
>gb|AC153120.10| Medicago truncatula clone mth2-20n3, WORKING DRAFT SEQUENCE, 3 ordered
pieces
Length = 119993
Score = 40.1 bits (20), Expect = 0.39
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 124 aaaacatgaccaggtgcaactcga 147
|||||||||| |||||||||||||
Sbjct: 54621 aaaacatgacaaggtgcaactcga 54644
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 239,054
Number of Sequences: 392609
Number of extensions: 239054
Number of successful extensions: 15754
Number of sequences better than 0.5: 14
Number of HSP's better than 0.5 without gapping: 14
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 15721
Number of HSP's gapped (non-prelim): 26
length of query: 1104
length of database: 441,732,993
effective HSP length: 20
effective length of query: 1084
effective length of database: 433,880,813
effective search space: 470326801292
effective search space used: 470326801292
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)