BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCG22g05.yg.2.1
(681 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AW698351.1|AW698351 g32 glandular-haired subtracted cDNA... 42 5e-004
gb|AW698352.1|AW698352 g34 glandular-haired subtracted cDNA... 42 5e-004
gb|AW698341.1|AW698341 G143 glandular-haired subtracted cDN... 40 0.002
gb|AW698345.1|AW698345 G73 glandular-haired subtracted cDNA... 40 0.002
gb|AW698347.1|AW698347 G78 glandular-haired subtracted cDNA... 40 0.002
gb|AW698350.1|AW698350 g29 glandular-haired subtracted cDNA... 40 0.002
gb|AW698672.1|AW698672 R103 non-glandular-haired subtracted... 40 0.002
gb|AW698675.1|AW698675 R10 non-glandular-haired subtracted ... 40 0.002
gb|AW698681.1|AW698681 R11 non-glandular-haired subtracted ... 40 0.002
gb|AW698683.1|AW698683 R121 non-glandular-haired subtracted... 40 0.002
gb|AW698685.1|AW698685 R125 non-glandular-haired subtracted... 40 0.002
gb|AW698688.1|AW698688 R12 non-glandular-haired subtracted ... 40 0.002
gb|AW698697.1|AW698697 R145 non-glandular-haired subtracted... 40 0.002
gb|AW698698.1|AW698698 R146 non-glandular-haired subtracted... 40 0.002
gb|AW698715.1|AW698715 R93 non-glandular-haired subtracted ... 40 0.002
gb|AW698716.1|AW698716 R95 non-glandular-haired subtracted ... 40 0.002
gb|AW698886.1|AW698886 r151 non-glandular-haired subtracted... 40 0.002
gb|AW698901.1|AW698901 r97 non-glandular-haired subtracted ... 40 0.002
gb|AW698338.1|AW698338 G1 glandular-haired subtracted cDNA ... 36 0.031
gb|AW698678.1|AW698678 R113 non-glandular-haired subtracted... 36 0.031
gb|AW698679.1|AW698679 R115 non-glandular-haired subtracted... 36 0.031
gb|AW698699.1|AW698699 R149 non-glandular-haired subtracted... 36 0.031
gb|AW698711.1|AW698711 R89 non-glandular-haired subtracted ... 36 0.031
gb|AW698717.1|AW698717 R96 non-glandular-haired subtracted ... 36 0.031
gb|AW698718.1|AW698718 R98 non-glandular-haired subtracted ... 36 0.031
gb|AW698895.1|AW698895 r3 non-glandular-haired subtracted c... 36 0.031
gb|AJ888497.1|AJ888497 AJ888497 Medicago sativa subsp. x va... 36 0.031
gb|AW698340.1|AW698340 G44 glandular-haired subtracted cDNA... 34 0.12
gb|AW698354.1|AW698354 g40 glandular-haired subtracted cDNA... 34 0.12
gb|AW698355.1|AW698355 g43 glandular-haired subtracted cDNA... 34 0.12
gb|AW698680.1|AW698680 R116 non-glandular-haired subtracted... 34 0.12
gb|AW698777.1|AW698777 r361 non-glandular-haired subtracted... 34 0.12
gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated... 34 0.12
gb|DQ122784.1| Medicago sativa clone C43 putative splicing ... 34 0.12
gb|AW698346.1|AW698346 G75 glandular-haired subtracted cDNA... 32 0.48
gb|AW698402.1|AW698402 G249 glandular-haired subtracted cDN... 32 0.48
gb|AW698490.1|AW698490 g454 glandular-haired subtracted cDN... 32 0.48
gb|AW698492.1|AW698492 g456 glandular-haired subtracted cDN... 32 0.48
gb|AW698526.1|AW698526 g320 glandular-haired subtracted cDN... 32 0.48
gb|AW698687.1|AW698687 R128 non-glandular-haired subtracted... 32 0.48
gb|AW698884.1|AW698884 r147 non-glandular-haired subtracted... 32 0.48
gb|AW698887.1|AW698887 r152 non-glandular-haired subtracted... 32 0.48
gb|AW698894.1|AW698894 r167 non-glandular-haired subtracted... 32 0.48
gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular tr... 32 0.48
gb|CO514053.1|CO514053 s13dSG72G0600040_156768 Glandular tr... 32 0.48
gb|AJ888498.1|AJ888498 AJ888498 Medicago sativa subsp. x va... 32 0.48
>gb|AW698351.1|AW698351 g32 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 327
Score = 42.1 bits (21), Expect = 5e-004
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtact 21
|||||||||||||||||||||
Sbjct: 310 gcggccgcccgggcaggtact 290
>gb|AW698352.1|AW698352 g34 glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 365
Score = 42.1 bits (21), Expect = 5e-004
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtact 21
|||||||||||||||||||||
Sbjct: 4 gcggccgcccgggcaggtact 24
>gb|AW698341.1|AW698341 G143 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 264
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 260 gcggccgcccgggcaggtac 241
>gb|AW698345.1|AW698345 G73 glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 407
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 5 gcggccgcccgggcaggtac 24
>gb|AW698347.1|AW698347 G78 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 553
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 93 gcggccgcccgggcaggtac 112
>gb|AW698350.1|AW698350 g29 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 274
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 270 gcggccgcccgggcaggtac 251
>gb|AW698672.1|AW698672 R103 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 251
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 5 gcggccgcccgggcaggtac 24
>gb|AW698675.1|AW698675 R10 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 289
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 5 gcggccgcccgggcaggtac 24
>gb|AW698681.1|AW698681 R11 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 168
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 5 gcggccgcccgggcaggtac 24
>gb|AW698683.1|AW698683 R121 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 527
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 5 gcggccgcccgggcaggtac 24
>gb|AW698685.1|AW698685 R125 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 393
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 389 gcggccgcccgggcaggtac 370
>gb|AW698688.1|AW698688 R12 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 356
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 4 gcggccgcccgggcaggtac 23
>gb|AW698697.1|AW698697 R145 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 354
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 5 gcggccgcccgggcaggtac 24
>gb|AW698698.1|AW698698 R146 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 326
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 4 gcggccgcccgggcaggtac 23
>gb|AW698715.1|AW698715 R93 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 440
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 5 gcggccgcccgggcaggtac 24
>gb|AW698716.1|AW698716 R95 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 434
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 4 gcggccgcccgggcaggtac 23
>gb|AW698886.1|AW698886 r151 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 129
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 5 gcggccgcccgggcaggtac 24
>gb|AW698901.1|AW698901 r97 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 212
Score = 40.1 bits (20), Expect = 0.002
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 208 gcggccgcccgggcaggtac 189
>gb|AW698338.1|AW698338 G1 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 483
Score = 36.2 bits (18), Expect = 0.031
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggt 18
||||||||||||||||||
Sbjct: 4 gcggccgcccgggcaggt 21
>gb|AW698678.1|AW698678 R113 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 252
Score = 36.2 bits (18), Expect = 0.031
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggt 18
||||||||||||||||||
Sbjct: 248 gcggccgcccgggcaggt 231
>gb|AW698679.1|AW698679 R115 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 418
Score = 36.2 bits (18), Expect = 0.031
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggt 18
||||||||||||||||||
Sbjct: 5 gcggccgcccgggcaggt 22
>gb|AW698699.1|AW698699 R149 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 275
Score = 36.2 bits (18), Expect = 0.031
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggt 18
||||||||||||||||||
Sbjct: 5 gcggccgcccgggcaggt 22
>gb|AW698711.1|AW698711 R89 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 343
Score = 36.2 bits (18), Expect = 0.031
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggt 18
||||||||||||||||||
Sbjct: 339 gcggccgcccgggcaggt 322
>gb|AW698717.1|AW698717 R96 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 384
Score = 36.2 bits (18), Expect = 0.031
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggt 18
||||||||||||||||||
Sbjct: 380 gcggccgcccgggcaggt 363
>gb|AW698718.1|AW698718 R98 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 330
Score = 36.2 bits (18), Expect = 0.031
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggt 18
||||||||||||||||||
Sbjct: 326 gcggccgcccgggcaggt 309
>gb|AW698895.1|AW698895 r3 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 370
Score = 36.2 bits (18), Expect = 0.031
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggt 18
||||||||||||||||||
Sbjct: 366 gcggccgcccgggcaggt 349
>gb|AJ888497.1|AJ888497 AJ888497 Medicago sativa subsp. x varia 4-day-old Medicago sativa
subsp. x varia cDNA, mRNA sequence
Length = 561
Score = 36.2 bits (18), Expect = 0.031
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggt 18
||||||||||||||||||
Sbjct: 555 gcggccgcccgggcaggt 538
>gb|AW698340.1|AW698340 G44 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 110
Score = 34.2 bits (17), Expect = 0.12
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 8 cccgggcaggtactctg 24
|||||||||||||||||
Sbjct: 103 cccgggcaggtactctg 87
>gb|AW698354.1|AW698354 g40 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 307
Score = 34.2 bits (17), Expect = 0.12
Identities = 20/21 (95%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtact 21
||||| |||||||||||||||
Sbjct: 280 gcggcagcccgggcaggtact 260
>gb|AW698355.1|AW698355 g43 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 168
Score = 34.2 bits (17), Expect = 0.12
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 5 ccgcccgggcaggtact 21
|||||||||||||||||
Sbjct: 168 ccgcccgggcaggtact 152
>gb|AW698680.1|AW698680 R116 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 290
Score = 34.2 bits (17), Expect = 0.12
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 5 ccgcccgggcaggtact 21
|||||||||||||||||
Sbjct: 283 ccgcccgggcaggtact 267
>gb|AW698777.1|AW698777 r361 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 340
Score = 34.2 bits (17), Expect = 0.12
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcagg 17
|||||||||||||||||
Sbjct: 328 gcggccgcccgggcagg 312
>gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated receptor-like kinase
mRNA, complete cds
Length = 1798
Score = 34.2 bits (17), Expect = 0.12
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 96 ccaaagtctgccacttt 112
|||||||||||||||||
Sbjct: 998 ccaaagtctgccacttt 982
>gb|DQ122784.1| Medicago sativa clone C43 putative splicing factor Prp8 mRNA,
partial cds
Length = 552
Score = 34.2 bits (17), Expect = 0.12
Identities = 20/21 (95%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtact 21
|||||||||||| ||||||||
Sbjct: 498 gcggccgcccggccaggtact 478
>gb|AW698346.1|AW698346 G75 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 225
Score = 32.2 bits (16), Expect = 0.48
Identities = 16/16 (100%)
Strand = Plus / Minus
Query: 5 ccgcccgggcaggtac 20
||||||||||||||||
Sbjct: 225 ccgcccgggcaggtac 210
>gb|AW698402.1|AW698402 G249 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 316
Score = 32.2 bits (16), Expect = 0.48
Identities = 16/16 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcag 16
||||||||||||||||
Sbjct: 304 gcggccgcccgggcag 289
>gb|AW698490.1|AW698490 g454 glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 115
Score = 32.2 bits (16), Expect = 0.48
Identities = 19/20 (95%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
|||||||||||| |||||||
Sbjct: 5 gcggccgcccggccaggtac 24
>gb|AW698492.1|AW698492 g456 glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 110
Score = 32.2 bits (16), Expect = 0.48
Identities = 19/20 (95%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
|||||||||||| |||||||
Sbjct: 5 gcggccgcccggccaggtac 24
>gb|AW698526.1|AW698526 g320 glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 223
Score = 32.2 bits (16), Expect = 0.48
Identities = 19/20 (95%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
|||||||||||| |||||||
Sbjct: 5 gcggccgcccggacaggtac 24
>gb|AW698687.1|AW698687 R128 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 477
Score = 32.2 bits (16), Expect = 0.48
Identities = 16/16 (100%)
Strand = Plus / Minus
Query: 3 ggccgcccgggcaggt 18
||||||||||||||||
Sbjct: 470 ggccgcccgggcaggt 455
>gb|AW698884.1|AW698884 r147 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 178
Score = 32.2 bits (16), Expect = 0.48
Identities = 16/16 (100%)
Strand = Plus / Plus
Query: 5 ccgcccgggcaggtac 20
||||||||||||||||
Sbjct: 10 ccgcccgggcaggtac 25
>gb|AW698887.1|AW698887 r152 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 198
Score = 32.2 bits (16), Expect = 0.48
Identities = 16/16 (100%)
Strand = Plus / Minus
Query: 7 gcccgggcaggtactc 22
||||||||||||||||
Sbjct: 187 gcccgggcaggtactc 172
>gb|AW698894.1|AW698894 r167 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 262
Score = 32.2 bits (16), Expect = 0.48
Identities = 16/16 (100%)
Strand = Plus / Plus
Query: 3 ggccgcccgggcaggt 18
||||||||||||||||
Sbjct: 8 ggccgcccgggcaggt 23
>gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 596
Score = 32.2 bits (16), Expect = 0.48
Identities = 16/16 (100%)
Strand = Plus / Minus
Query: 128 gtctaggagaatgtta 143
||||||||||||||||
Sbjct: 351 gtctaggagaatgtta 336
>gb|CO514053.1|CO514053 s13dSG72G0600040_156768 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 552
Score = 32.2 bits (16), Expect = 0.48
Identities = 16/16 (100%)
Strand = Plus / Minus
Query: 301 gcatgaactcatacac 316
||||||||||||||||
Sbjct: 490 gcatgaactcatacac 475
>gb|AJ888498.1|AJ888498 AJ888498 Medicago sativa subsp. x varia 4-day-old Medicago sativa
subsp. x varia cDNA, mRNA sequence
Length = 530
Score = 32.2 bits (16), Expect = 0.48
Identities = 16/16 (100%)
Strand = Plus / Minus
Query: 5 ccgcccgggcaggtac 20
||||||||||||||||
Sbjct: 522 ccgcccgggcaggtac 507
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1977
Number of Sequences: 7669
Number of extensions: 1977
Number of successful extensions: 519
Number of sequences better than 0.5: 47
Number of HSP's better than 0.5 without gapping: 47
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 470
Number of HSP's gapped (non-prelim): 49
length of query: 681
length of database: 3,745,706
effective HSP length: 16
effective length of query: 665
effective length of database: 3,623,002
effective search space: 2409296330
effective search space used: 2409296330
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)