BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2943966.2.1
         (599 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AF332134.1|  Medicago sativa FtsH protease (FtsH) mRNA, c...    34   0.11 
gb|AW698451.1|AW698451  g402 glandular-haired subtracted cDN...    32   0.43 
gb|AW698482.1|AW698482  g443 glandular-haired subtracted cDN...    32   0.43 

>gb|AF332134.1| Medicago sativa FtsH protease (FtsH) mRNA, complete cds; chloroplast
            gene for chloroplast product
          Length = 2352

 Score = 34.2 bits (17), Expect = 0.11
 Identities = 20/21 (95%)
 Strand = Plus / Plus

                                 
Query: 488  gaaggcgaagtcgaaggcgcc 508
            |||||||||||| ||||||||
Sbjct: 1069 gaaggcgaagtcaaaggcgcc 1089



>gb|AW698451.1|AW698451 g402 glandular-haired subtracted cDNA library Medicago sativa cDNA,
           mRNA sequence
          Length = 318

 Score = 32.2 bits (16), Expect = 0.43
 Identities = 16/16 (100%)
 Strand = Plus / Plus

                           
Query: 190 agccatgcatgcatgc 205
           ||||||||||||||||
Sbjct: 31  agccatgcatgcatgc 46



>gb|AW698482.1|AW698482 g443 glandular-haired subtracted cDNA library Medicago sativa cDNA,
           mRNA sequence
          Length = 127

 Score = 32.2 bits (16), Expect = 0.43
 Identities = 16/16 (100%)
 Strand = Plus / Plus

                           
Query: 190 agccatgcatgcatgc 205
           ||||||||||||||||
Sbjct: 32  agccatgcatgcatgc 47



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 476
Number of Sequences: 7669
Number of extensions: 476
Number of successful extensions: 154
Number of sequences better than  0.5: 3
Number of HSP's better than  0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 149
Number of HSP's gapped (non-prelim): 5
length of query: 599
length of database: 3,745,706
effective HSP length: 16
effective length of query: 583
effective length of database: 3,623,002
effective search space: 2112210166
effective search space used: 2112210166
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)