BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419471.2.6
(1648 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY560003.1| Medicago sativa S-adenosylmethionine synthas... 448 e-126
gb|CO514605.1|CO514605 s13dSG42G0700056_327870 Glandular tr... 238 1e-062
gb|CO516632.1|CO516632 s13dSG66E0200007_446592 Glandular tr... 180 2e-045
gb|CO514747.1|CO514747 s13dSG55F1100095_328154 Glandular tr... 170 2e-042
gb|AW698879.1|AW698879 r109 non-glandular-haired subtracted... 80 6e-015
gb|CO514483.1|CO514483 s13dSG43C0600040_327626 Glandular tr... 46 8e-005
gb|CO516330.1|CO516330 s13dSG99B0800063_445988 Glandular tr... 46 8e-005
emb|Z50800.1|MSASET2MR M.sativa mRNA for ASET2 42 0.001
gb|AA735028.1|AA735028 EST00008 young root nodules Medicago... 34 0.30
gb|AJ410110.1|AJ410110 AJ410110 Medicago sativa library (Ka... 34 0.30
gb|CO512284.1|CO512284 s13dSG68B0900073_114082 Glandular tr... 34 0.30
gb|CO516526.1|CO516526 s13dSG57B0400029_446380 Glandular tr... 34 0.30
>gb|AY560003.1| Medicago sativa S-adenosylmethionine synthase mRNA, complete cds
Length = 1173
Score = 448 bits (226), Expect = e-126
Identities = 673/822 (81%)
Strand = Plus / Plus
Query: 201 ttcacctcggagtctgtgaacgagggacaccctgacaagctctgtgaccaggtctcagat 260
|||||||| ||||| ||||| ||||| || || |||||||| ||||| || |||| |||
Sbjct: 16 ttcacctctgagtccgtgaatgagggtcatcccgacaagctttgtgatcaaatctctgat 75
Query: 261 gccgttcttgacgcttgccttgctgaggaccctgacagcaaggttgcttgtgagacctgc 320
|| || || || |||||||||| ||||| |||||||||||||| |||||||| |||
Sbjct: 76 gctgtgctagatgcttgccttgaacaggacgtagacagcaaggttgcatgtgagacttgc 135
Query: 321 accaagaccaacatggtcatggtctttggtgagatcaccaccaaggccaatgttgactac 380
|| |||||||||||||| |||||||||||||||||||| |||||||| || ||||||||
Sbjct: 136 actaagaccaacatggttatggtctttggtgagatcacaaccaaggctaaggttgactat 195
Query: 381 gagaagattgtcagggagacctgccgcaacattggttttgtgtcaaacgatgttgggctt 440
||||| ||||| | || ||||| | || ||||| ||||| || | ||||| || |||
Sbjct: 196 gagaaaattgtgcgtgatacctgtagaaagattgggtttgtttctgatgatgtaggtctt 255
Query: 441 gacgccgaccactgcaaggtgctcgtgaacattgagcagcagtcccctgatattgctcag 500
|| || ||| |||||||||| || || ||||||||||||||| |||||||||||||||
Sbjct: 256 gatgctgacaactgcaaggttcttgtcaacattgagcagcagagtcctgatattgctcag 315
Query: 501 ggtgtgcatggccacttcaccaagcgccccgaggagattggagctggtgaccagggacac 560
||||| ||||| || | || || | || ||||||||||| |||||||||||||| |||
Sbjct: 316 ggtgtacatggtcatcttacaaaaagacctgaggagattggtgctggtgaccagggtcac 375
Query: 561 atgttcgggtatgcgaccgatgagacccctgagttgatgcccctcagccatgtccttgcc 620
||||| || ||||| || |||||||| ||||| |||||||| | |||||||| |||||
Sbjct: 376 atgtttggctatgctactgatgagactcctgaattgatgcctttgagccatgttcttgca 435
Query: 621 accaagctaggtgctcgtctcaccgaggtccgcaagaacggaacctgcccctggctcagg 680
|||||||| |||||||||||||| ||||| |||||||| || || ||||| ||| | ||
Sbjct: 436 accaagcttggtgctcgtctcactgaggttcgcaagaatggtacatgcccttggttgaga 495
Query: 681 cctgatgggaagacccaggtgacagtcgagtaccgcaatgagggtggtgccatggtcccc 740
|||||||| ||||| || || || || ||||| |||||| |||||||||||||||
Sbjct: 496 cctgatggtaagactcaagtcactgttgagtattacaatgacaatggtgccatggtccct 555
Query: 741 atccgtgtccacaccgtcctcatctccacccagcacgacgagacagtgaccaatgatgag 800
| ||||||||||| || |||||||| || || || || ||||| || |||||||||||
Sbjct: 556 gttcgtgtccacactgtgctcatctctactcaacatgatgagactgttaccaatgatgaa 615
Query: 801 atcgctgctgacctgaaggagcatgtcatcaagcctgtcatccctgagcagtaccttgac 860
|| |||||||| || || |||||||| || |||||||| || |||||| ||||||||||
Sbjct: 616 attgctgctgatctcaaagagcatgttataaagcctgtgattcctgagaagtaccttgat 675
Query: 861 gagaagaccatcttccaccttaacccatctggccgctttgtcattggtggacctcacggc 920
|||||||| || ||| | ||||| |||||||| || |||||||| || ||||||||
Sbjct: 676 tccaagaccatttttcacttgaacccttctggccgtttcgtcattggaggtcctcacggt 735
Query: 921 gatgctggcctcactggccgcaagatcatcattgacacctacggtggctggggagcccat 980
|||||||| |||||||| | ||||||||||||||||| || ||||| ||||| || |||
Sbjct: 736 gatgctggtctcactggtaggaagatcatcattgacacttatggtggttggggtgcacat 795
Query: 981 ggtggtggcgctttctccggcaaggacccaaccaaggttgac 1022
|||||||| |||||||| || || |||||||| |||||||||
Sbjct: 796 ggtggtggtgctttctcaggaaaagacccaactaaggttgac 837
Score = 75.8 bits (38), Expect = 9e-014
Identities = 68/78 (87%)
Strand = Plus / Plus
Query: 1166 gatccccgacaaggagatcctcaagattgtcaaggagaacttcgatttcaggcctggcat 1225
|||||| || ||||| |||||||| ||||| ||| ||||||| |||||||||||||||||
Sbjct: 981 gatccctgataaggaaatcctcaatattgtgaagcagaactttgatttcaggcctggcat 1040
Query: 1226 gatcatcatcaaccttga 1243
|||| |||||||||||
Sbjct: 1041 gatctctatcaaccttga 1058
>gb|CO514605.1|CO514605 s13dSG42G0700056_327870 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 600
Score = 238 bits (120), Expect = 1e-062
Identities = 369/452 (81%)
Strand = Plus / Plus
Query: 213 tctgtgaacgagggacaccctgacaagctctgtgaccaggtctcagatgccgttcttgac 272
|||||||||||||||||||| |||||||| || |||||| |||| ||||||||||||||
Sbjct: 146 tctgtgaacgagggacaccccgacaagctttgcgaccagatctctgatgccgttcttgat 205
Query: 273 gcttgccttgctgaggaccctgacagcaaggttgcttgtgagacctgcaccaagaccaac 332
|||||||| | | || || |||||||| ||||| ||||| || ||||| |||||||||
Sbjct: 206 gcttgcctcgagcaagatccagacagcaaagttgcatgtgaaacttgcacaaagaccaac 265
Query: 333 atggtcatggtctttggtgagatcaccaccaaggccaatgttgactacgagaagattgtc 392
||||| |||||||||||||||||||| || || || ||||||||||| || |||||||||
Sbjct: 266 atggttatggtctttggtgagatcacaacaaaagcaaatgttgactatgaaaagattgtc 325
Query: 393 agggagacctgccgcaacattggttttgtgtcaaacgatgttgggcttgacgccgaccac 452
| | || ||| | ||||| |||||||| ||| |||||||||| ||||| || ||| ||
Sbjct: 326 cgtaacacatgcaggaacataggttttgtttcagacgatgttggtcttgatgctgacaac 385
Query: 453 tgcaaggtgctcgtgaacattgagcagcagtcccctgatattgctcagggtgtgcatggc 512
||||| || || || |||||||| || ||| |||||||||||||| ||||| ||||||
Sbjct: 386 tgcaaagtccttgtcaacattgaacaacagagtcctgatattgctcaaggtgttcatggc 445
Query: 513 cacttcaccaagcgccccgaggagattggagctggtgaccagggacacatgttcgggtat 572
|| | || | | || ||||| ||||| |||||||| || || |||||||||||||||
Sbjct: 446 catcttacagaaagacctgaggaaattggtgctggtgatcaaggtcacatgttcgggtat 505
Query: 573 gcgaccgatgagacccctgagttgatgcccctcagccatgtccttgccaccaagctaggt 632
|| |||||||| || |||||| | ||||| | | |||||| ||||| |||||| ||||
Sbjct: 506 gctaccgatgaaacacctgagcttatgcctttaacccatgttcttgcaaccaagttagga 565
Query: 633 gctcgtctcaccgaggtccgcaagaacggaac 664
||| ||||| || || ||||||||||||||
Sbjct: 566 gctaagctcactgaagttcgcaagaacggaac 597
>gb|CO516632.1|CO516632 s13dSG66E0200007_446592 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 425
Score = 180 bits (91), Expect = 2e-045
Identities = 220/263 (83%)
Strand = Plus / Plus
Query: 213 tctgtgaacgagggacaccctgacaagctctgtgaccaggtctcagatgccgttcttgac 272
|||||||||||||||||||| |||||||| || |||||| |||| ||||||||||||||
Sbjct: 146 tctgtgaacgagggacaccccgacaagctttgcgaccagatctctgatgccgttcttgat 205
Query: 273 gcttgccttgctgaggaccctgacagcaaggttgcttgtgagacctgcaccaagaccaac 332
|||||||| | | || || |||||||| ||||| ||||| || ||||| |||||||||
Sbjct: 206 gcttgcctcgagcaagatccagacagcaaagttgcatgtgaaacttgcacaaagaccaac 265
Query: 333 atggtcatggtctttggtgagatcaccaccaaggccaatgttgactacgagaagattgtc 392
||||| |||||||||||||||||||| || || || ||||||||||| || |||||||||
Sbjct: 266 atggttatggtctttggtgagatcacaacaaaagcaaatgttgactatgaaaagattgtc 325
Query: 393 agggagacctgccgcaacattggttttgtgtcaaacgatgttgggcttgacgccgaccac 452
| | || ||| | ||||| |||||||| ||| |||||||||| ||||| || ||| ||
Sbjct: 326 cgtaacacatgcaggaacataggttttgtttcagacgatgttggtcttgatgctgacaac 385
Query: 453 tgcaaggtgctcgtgaacattga 475
||||| || || || ||||||||
Sbjct: 386 tgcaaagtccttgtcaacattga 408
>gb|CO514747.1|CO514747 s13dSG55F1100095_328154 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 435
Score = 170 bits (86), Expect = 2e-042
Identities = 233/282 (82%)
Strand = Plus / Plus
Query: 216 gtgaacgagggacaccctgacaagctctgtgaccaggtctcagatgccgttcttgacgct 275
||||||||||| ||||| |||||||| || |||||| |||| ||||| || || ||||||
Sbjct: 154 gtgaacgagggtcaccccgacaagctgtgcgaccagatctctgatgcagtgctcgacgct 213
Query: 276 tgccttgctgaggaccctgacagcaaggttgcttgtgagacctgcaccaagaccaacatg 335
|| |||| |||||||||||||||||| ||| |||||||| ||||||||||| ||||||
Sbjct: 214 tgtcttgagcaggaccctgacagcaagggtgcctgtgagacatgcaccaagactaacatg 273
Query: 336 gtcatggtctttggtgagatcaccaccaaggccaatgttgactacgagaagattgtcagg 395
|||||||||||||| || || || ||||||||||| || ||||| |||||||| || |
Sbjct: 274 gtcatggtctttggagaaataacaaccaaggccaacgtagactatgagaagatcgttcgt 333
Query: 396 gagacctgccgcaacattggttttgtgtcaaacgatgttgggcttgacgccgaccactgc 455
|| || ||||||| |||||| || | || | |||||||| ||||| |||||| | |||
Sbjct: 334 gacacatgccgcaccattggattcatctctgatgatgttggtcttgatgccgacaaatgc 393
Query: 456 aaggtgctcgtgaacattgagcagcagtcccctgatattgct 497
||||| | || ||||||||||||||| ||||||||||||
Sbjct: 394 aaggtcttggtcaacattgagcagcagagtcctgatattgct 435
>gb|AW698879.1|AW698879 r109 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 205
Score = 79.8 bits (40), Expect = 6e-015
Identities = 61/68 (89%)
Strand = Plus / Plus
Query: 933 actggccgcaagatcatcattgacacctacggtggctggggagcccatggtggtggcgct 992
|||||||||||||||||||| || ||||||||||| ||||| || || |||||||| |||
Sbjct: 3 actggccgcaagatcatcatcgatacctacggtggttggggtgcacacggtggtggtgct 62
Query: 993 ttctccgg 1000
||||||||
Sbjct: 63 ttctccgg 70
>gb|CO514483.1|CO514483 s13dSG43C0600040_327626 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 505
Score = 46.1 bits (23), Expect = 8e-005
Identities = 32/35 (91%)
Strand = Plus / Plus
Query: 1312 ctgacttcacctgggaggtggtgaagccactcaag 1346
|||| ||||| |||||||| |||||||||||||||
Sbjct: 334 ctgatttcacatgggaggttgtgaagccactcaag 368
>gb|CO516330.1|CO516330 s13dSG99B0800063_445988 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 388
Score = 46.1 bits (23), Expect = 8e-005
Identities = 32/35 (91%)
Strand = Plus / Plus
Query: 1312 ctgacttcacctgggaggtggtgaagccactcaag 1346
|||| ||||| |||||||| |||||||||||||||
Sbjct: 73 ctgatttcacatgggaggttgtgaagccactcaag 107
>emb|Z50800.1|MSASET2MR M.sativa mRNA for ASET2
Length = 1193
Score = 42.1 bits (21), Expect = 0.001
Identities = 31/33 (93%), Gaps = 1/33 (3%)
Strand = Plus / Plus
Query: 1 atcccccgggctgtatggaattcggcacgaggc 33
||||||||||||| | |||||||||||||||||
Sbjct: 36 atcccccgggctgca-ggaattcggcacgaggc 67
>gb|AA735028.1|AA735028 EST00008 young root nodules Medicago sativa subsp. x varia cDNA clone
Mscp12 5' similar to glutamine synthetase homologue, mRNA
sequence
Length = 290
Score = 34.2 bits (17), Expect = 0.30
Identities = 17/17 (100%)
Strand = Plus / Plus
Query: 1231 tcatcaaccttgacctc 1247
|||||||||||||||||
Sbjct: 18 tcatcaaccttgacctc 34
>gb|AJ410110.1|AJ410110 AJ410110 Medicago sativa library (Kalo P) Medicago sativa cDNA
clone U281, mRNA sequence
Length = 754
Score = 34.2 bits (17), Expect = 0.30
Identities = 27/29 (93%), Gaps = 1/29 (3%)
Strand = Plus / Plus
Query: 4 ccccgggctgtatggaattcggcacgagg 32
|||||||||| | ||||||||||||||||
Sbjct: 30 ccccgggctgca-ggaattcggcacgagg 57
>gb|CO512284.1|CO512284 s13dSG68B0900073_114082 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 534
Score = 34.2 bits (17), Expect = 0.30
Identities = 20/21 (95%)
Strand = Plus / Plus
Query: 503 tgtgcatggccacttcaccaa 523
|||||||||||| ||||||||
Sbjct: 232 tgtgcatggccatttcaccaa 252
>gb|CO516526.1|CO516526 s13dSG57B0400029_446380 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 430
Score = 34.2 bits (17), Expect = 0.30
Identities = 17/17 (100%)
Strand = Plus / Plus
Query: 1231 tcatcaaccttgacctc 1247
|||||||||||||||||
Sbjct: 153 tcatcaaccttgacctc 169
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 2912
Number of Sequences: 7669
Number of extensions: 2912
Number of successful extensions: 745
Number of sequences better than 0.5: 12
Number of HSP's better than 0.5 without gapping: 11
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 714
Number of HSP's gapped (non-prelim): 25
length of query: 1648
length of database: 3,745,706
effective HSP length: 16
effective length of query: 1632
effective length of database: 3,623,002
effective search space: 5912739264
effective search space used: 5912739264
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)