BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192958.2.3
(1681 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ248336.1|AJ248336 AJ248336 Medicago sativa subsp. x va... 204 1e-052
gb|AJ966532.1|AJ966532 AJ966532 Medicago sativa subsp. falc... 66 9e-011
gb|CO513103.1|CO513103 s13dSG89H0300028_122132 Glandular tr... 60 5e-009
gb|AJ966338.1|AJ966338 AJ966338 Medicago sativa subsp. falc... 58 2e-008
gb|AW698402.1|AW698402 G249 glandular-haired subtracted cDN... 54 3e-007
gb|AW698418.1|AW698418 G266 glandular-haired subtracted cDN... 52 1e-006
gb|AF069507.1|AF069507 Medicago sativa DnaJ-like protein Ms... 50 5e-006
emb|AJ000995.1|MSDNAJ Medicago sativa mRNA for DnaJ-like pr... 50 5e-006
emb|Z71997.1|MSDNAJLP M.sativa mRNA for DnaJ-like protein 42 0.001
>gb|AJ248336.1|AJ248336 AJ248336 Medicago sativa subsp. x varia young root nodule Medicago
sativa subsp. x varia cDNA clone MsNod658, mRNA sequence
Length = 1004
Score = 204 bits (103), Expect = 1e-052
Identities = 388/483 (80%)
Strand = Plus / Minus
Query: 601 gggaactccaccgagaagtggatgtacagcttgcccttcatgaacggcctctggtacatg 660
||||| ||||| | |||||| ||||| |||||||||||||| || ||||||||||||||
Sbjct: 556 gggaattccacagtgaagtgaatgtaaagcttgcccttcataaatggcctctggtacatc 497
Query: 661 ggcatgccttcgtcgttgatcgccttgaaagaatcaggcttcacaacttcgcccggattt 720
||||| || || ||||| || |||||| | ||||||||||| |||||||| || || ||
Sbjct: 496 ggcattccctcatcgttaatagccttgtatgaatcaggcttgacaacttctccggggttc 437
Query: 721 gatttgatcagcagctgcctgttatccaagtgagtcacaacaaactggaagccacacaga 780
|||||||| || || || | |||||||||||| | ||||| ||||| ||||||| |
Sbjct: 436 gatttgataagaagttggcgaccatccaagtgagtaagcacaaattggaaaccacacaaa 377
Query: 781 gattcagtcaaggtcagggtgtgctcgtagaagaggtcatcaccctttctcttgaacttg 840
| ||||| | | ||| |||||||| || ||||| |||| |||||| |||||||||
Sbjct: 376 gcctcagtaagagacagtgtgtgctctacaaaaaggtcttcactctttcttttgaacttg 317
Query: 841 ggatgctccttctgctggagaacaaagatgatatctccagtgacagtgtcaggcgcttca 900
|| || || |||||||| || ||||| | ||||| ||||| || ||||| || ||||||
Sbjct: 316 gggtgttctttctgctgaagcacaaatacaatatccccagtaactgtgtctggtgcttca 257
Query: 901 tcagcttcaccagggaatgtgatcttctgcccattctgcatgcctttctccacaaacact 960
|| ||||||||||||||||| |||||||| | |||||||| |||||||| ||| |||
Sbjct: 256 tcggcttcaccagggaatgtaatcttctgactgttctgcattcctttctcgacatgaact 197
Query: 961 tcaagaaccttcttctcctgcacaactttatcacccttgcactgtgggcatctatccttg 1020
||||| || |||||||| || ||||| || || |||||||||||||| || | || ||
Sbjct: 196 tcaagtactttcttctcttgtacaaccttctctcccttgcactgtggacagcggtcttta 137
Query: 1021 tcgctgattgtctctccagaacccttgcactcgttgcaaggatgctgcatttgctggatc 1080
|| |||| || || || | |||||| || || |||||||||||||||||||||||||||
Sbjct: 136 tcattgatagtttcaccggtacccttacattcattgcaaggatgctgcatttgctggatc 77
Query: 1081 att 1083
|||
Sbjct: 76 att 74
Score = 65.9 bits (33), Expect = 9e-011
Identities = 69/81 (85%)
Strand = Plus / Minus
Query: 375 ctactgctgggcgcactgcaccctctgcgcgccgcccggcatgtcctcatcctcgtcgta 434
|||||||||||||||||| |||||||| || || || ||||| || ||||||||||| ||
Sbjct: 782 ctactgctgggcgcactgtaccctctgtgccccaccaggcatatcatcatcctcgtcata 723
Query: 435 ggcctcctggtgctgctgctg 455
|| ||||| || ||||||||
Sbjct: 722 tgcttcctgttgttgctgctg 702
>gb|AJ966532.1|AJ966532 AJ966532 Medicago sativa subsp. falcata flower buds Medicago sativa
subsp. falcata cDNA clone Em52, mRNA sequence
Length = 96
Score = 65.9 bits (33), Expect = 9e-011
Identities = 52/57 (91%), Gaps = 1/57 (1%)
Strand = Plus / Minus
Query: 871 atatctccagtgac-agtgtcaggcgcttcatcagcttcaccagggaatgtgatctt 926
||||||||||| || ||||||||| |||||||| |||||||||||||| ||||||||
Sbjct: 61 atatctccagtaaccagtgtcaggagcttcatcggcttcaccagggaaggtgatctt 5
>gb|CO513103.1|CO513103 s13dSG89H0300028_122132 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 550
Score = 60.0 bits (30), Expect = 5e-009
Identities = 63/74 (85%)
Strand = Plus / Minus
Query: 1204 agagagagcttctttgatgtgccattgtacaaatcctccagagaaaccttcagaggatga 1263
||||| |||||||| ||||| ||| ||| | |||||||| ||| ||||| |||||||||
Sbjct: 500 agagaaagcttcttagatgttccaaggtaaagatcctccaaagagaccttgagaggatga 441
Query: 1264 accacatcatctcc 1277
|||||||| |||||
Sbjct: 440 accacatcttctcc 427
Score = 54.0 bits (27), Expect = 3e-007
Identities = 87/107 (81%)
Strand = Plus / Minus
Query: 1381 cccattccctccttgagggcatcctcaccatattggtcatagatctctctcttttcaggg 1440
|||||||| ||||| || ||||||||||||||| ||||| || || | ||| |||||
Sbjct: 329 cccattccttccttaagagcatcctcaccatatgtatcataaatatcacgcttctcagga 270
Query: 1441 tcgctgaggacctcataaccctgagctagctccttgaacttctcggg 1487
|| || | ||||||||| | || |||||||| ||||||||||||||
Sbjct: 269 tcactcaaaacctcataagcttgtgctagctctttgaacttctcggg 223
>gb|AJ966338.1|AJ966338 AJ966338 Medicago sativa subsp. falcata flower buds Medicago sativa
subsp. falcata cDNA clone Em53, mRNA sequence
Length = 96
Score = 58.0 bits (29), Expect = 2e-008
Identities = 51/57 (89%), Gaps = 1/57 (1%)
Strand = Plus / Minus
Query: 871 atatctccagtgacagtgtcaggc-gcttcatcagcttcaccagggaatgtgatctt 926
||||||||||| || |||||||| |||||||| |||||||||||||| ||||||||
Sbjct: 61 atatctccagtaaccgtgtcaggaagcttcatcggcttcaccagggaaggtgatctt 5
>gb|AW698402.1|AW698402 G249 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 316
Score = 54.0 bits (27), Expect = 3e-007
Identities = 39/43 (90%)
Strand = Plus / Plus
Query: 616 aagtggatgtacagcttgcccttcatgaacggcctctggtaca 658
||||| ||||| |||||||||||||| || |||||||||||||
Sbjct: 246 aagtgaatgtaaagcttgcccttcataaatggcctctggtaca 288
>gb|AW698418.1|AW698418 G266 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 273
Score = 52.0 bits (26), Expect = 1e-006
Identities = 38/42 (90%)
Strand = Plus / Plus
Query: 616 aagtggatgtacagcttgcccttcatgaacggcctctggtac 657
||||| ||||| |||||||||||||| || ||||||||||||
Sbjct: 226 aagtgaatgtaaagcttgcccttcataaatggcctctggtac 267
>gb|AF069507.1|AF069507 Medicago sativa DnaJ-like protein MsJ1 gene, complete cds
Length = 4449
Score = 50.1 bits (25), Expect = 5e-006
Identities = 55/65 (84%)
Strand = Plus / Minus
Query: 1381 cccattccctccttgagggcatcctcaccatattggtcatagatctctctcttttcaggg 1440
|||||||| || || |||||||| ||||||||||| ||||| | ||| ||||||||||
Sbjct: 1079 cccattccttctttaagggcatcttcaccatattgatcatacagttctttcttttcagga 1020
Query: 1441 tcgct 1445
|||||
Sbjct: 1019 tcgct 1015
Score = 34.2 bits (17), Expect = 0.31
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 634 cccttcatgaacggcct 650
|||||||||||||||||
Sbjct: 3255 cccttcatgaacggcct 3239
>emb|AJ000995.1|MSDNAJ Medicago sativa mRNA for DnaJ-like protein
Length = 1545
Score = 50.1 bits (25), Expect = 5e-006
Identities = 55/65 (84%)
Strand = Plus / Minus
Query: 1381 cccattccctccttgagggcatcctcaccatattggtcatagatctctctcttttcaggg 1440
|||||||| || || |||||||| ||||||||||| ||||| | ||| ||||||||||
Sbjct: 308 cccattccttctttaagggcatcttcaccatattgatcatacagttctttcttttcagga 249
Query: 1441 tcgct 1445
|||||
Sbjct: 248 tcgct 244
Score = 34.2 bits (17), Expect = 0.31
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 634 cccttcatgaacggcct 650
|||||||||||||||||
Sbjct: 1058 cccttcatgaacggcct 1042
>emb|Z71997.1|MSDNAJLP M.sativa mRNA for DnaJ-like protein
Length = 513
Score = 42.1 bits (21), Expect = 0.001
Identities = 36/41 (87%)
Strand = Plus / Minus
Query: 1381 cccattccctccttgagggcatcctcaccatattggtcata 1421
|||||||| || || |||||||| ||||||||||| |||||
Sbjct: 67 cccattccttctttaagggcatcttcaccatattgatcata 27
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 3936
Number of Sequences: 7669
Number of extensions: 3936
Number of successful extensions: 1192
Number of sequences better than 0.5: 9
Number of HSP's better than 0.5 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1160
Number of HSP's gapped (non-prelim): 31
length of query: 1681
length of database: 3,745,706
effective HSP length: 16
effective length of query: 1665
effective length of database: 3,623,002
effective search space: 6032298330
effective search space used: 6032298330
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)