BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192958.2.2
(1723 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ248336.1|AJ248336 AJ248336 Medicago sativa subsp. x va... 210 2e-054
gb|CO513103.1|CO513103 s13dSG89H0300028_122132 Glandular tr... 147 3e-035
gb|AF069507.1|AF069507 Medicago sativa DnaJ-like protein Ms... 66 9e-011
emb|AJ000995.1|MSDNAJ Medicago sativa mRNA for DnaJ-like pr... 66 9e-011
emb|Z71997.1|MSDNAJLP M.sativa mRNA for DnaJ-like protein 66 9e-011
gb|AJ966338.1|AJ966338 AJ966338 Medicago sativa subsp. falc... 64 4e-010
gb|AJ966532.1|AJ966532 AJ966532 Medicago sativa subsp. falc... 64 4e-010
gb|AW698418.1|AW698418 G266 glandular-haired subtracted cDN... 56 9e-008
gb|AW698402.1|AW698402 G249 glandular-haired subtracted cDN... 44 3e-004
gb|CO515218.1|CO515218 s13dSG96A0700053_399301 Glandular tr... 36 0.080
gb|AF201458.1|AF201458 Medicago sativa isoflavone reductase... 34 0.31
gb|M74191.1|ALFESIPB Medicago sativa environmental stress-i... 32 1.2
gb|AJ410119.1|AJ410119 AJ410119 Medicago sativa library (Ka... 30 4.9
gb|CO511943.1|CO511943 s13dSG05H0300032_103786 Glandular tr... 30 4.9
gb|CO513167.1|CO513167 s13dSG23F0700059_129498 Glandular tr... 30 4.9
gb|CO513484.1|CO513484 s13dSG10G1200088_130132 Glandular tr... 30 4.9
gb|CO513968.1|CO513968 s13dSG71G0500036_156598 Glandular tr... 30 4.9
gb|CO514716.1|CO514716 s13dSG55C0600050_328092 Glandular tr... 30 4.9
gb|CO514968.1|CO514968 s13dSG63F0600059_397441 Glandular tr... 30 4.9
gb|CO516175.1|CO516175 s13dSG67C0900070_445678 Glandular tr... 30 4.9
gb|CO516573.1|CO516573 s13dSG57G0500037_446474 Glandular tr... 30 4.9
gb|L39960.1|ALFRPE15A Medicago sativa middle repetitive DNA... 30 4.9
emb|AJ237729.1|MSA237729 Medicago sativa DNA for putative m... 30 4.9
gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase ... 30 4.9
gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase ... 30 4.9
gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase ... 30 4.9
gb|AF439380.1| Medicago sativa retrotransposon polyprotein ... 30 4.9
>gb|AJ248336.1|AJ248336 AJ248336 Medicago sativa subsp. x varia young root nodule Medicago
sativa subsp. x varia cDNA clone MsNod658, mRNA sequence
Length = 1004
Score = 210 bits (106), Expect = 2e-054
Identities = 391/486 (80%)
Strand = Plus / Minus
Query: 638 gtcggggaactccaccgtgaaatgaatgtacagcttccccttcatgaaaggcctctggta 697
||||||||| ||||| ||||| |||||||| ||||| |||||||| || |||||||||||
Sbjct: 560 gtcggggaattccacagtgaagtgaatgtaaagcttgcccttcataaatggcctctggta 501
Query: 698 aatcggcatcccctcatcgtttattgccttgaattggtcaggtttaacaacttcaccagg 757
|||||||| ||||||||||| || |||||| || ||||| || |||||||| || ||
Sbjct: 500 catcggcattccctcatcgttaatagccttgtatgaatcaggcttgacaacttctccggg 441
Query: 758 gtctgatttgatgagaagctgcctgttgtccagatgtgtaagaacaaattggaacccaca 817
|| |||||||| ||||| || | |||| || ||||| ||||||||||| |||||
Sbjct: 440 gttcgatttgataagaagttggcgaccatccaagtgagtaagcacaaattggaaaccaca 381
Query: 818 tagtgcttcggtcagagacaaggtgtgctcatagaagagatcttcaccctttcttttgaa 877
| || || || ||||||| |||||||| || || ||||||| ||||||||||||
Sbjct: 380 caaagcctcagtaagagacagtgtgtgctctacaaaaaggtcttcactctttcttttgaa 321
Query: 878 cttggagtggtccttctgctggaggacgaatacaatgtctccagtaacggtatcaggcgc 937
||||| ||| || |||||||| || || |||||||| || |||||||| || || || ||
Sbjct: 320 cttggggtgttctttctgctgaagcacaaatacaatatccccagtaactgtgtctggtgc 261
Query: 938 ttcatcagcttcaccagggaaggtgatcttctggctgtgttgcattcccttctcaacatg 997
|||||| |||||||||||||| || |||||||| |||| |||||||| ||||| |||||
Sbjct: 260 ttcatcggcttcaccagggaatgtaatcttctgactgttctgcattcctttctcgacatg 201
Query: 998 aacctcaagaaccttcttctcctgaacgaccttctcacccttacaccctgggcagcggtc 1057
||| ||||| || |||||||| || || |||||||| ||||| ||| ||| ||||||||
Sbjct: 200 aacttcaagtactttcttctcttgtacaaccttctctcccttgcactgtggacagcggtc 141
Query: 1058 cttctcgttgatgctctccccagtccccttgcactcgttgcaaggctgctgcatctgctg 1117
|| || ||||| | || || || ||||| || || |||||||| |||||||| |||||
Sbjct: 140 tttatcattgatagtttcaccggtacccttacattcattgcaaggatgctgcatttgctg 81
Query: 1118 tatcat 1123
|||||
Sbjct: 80 gatcat 75
Score = 46.1 bits (23), Expect = 8e-005
Identities = 29/31 (93%)
Strand = Plus / Minus
Query: 543 tcctcgcattcatctatctccatgtctgtca 573
|||||||| ||||| ||||||||||||||||
Sbjct: 655 tcctcgcactcatcgatctccatgtctgtca 625
>gb|CO513103.1|CO513103 s13dSG89H0300028_122132 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 550
Score = 147 bits (74), Expect = 3e-035
Identities = 128/146 (87%)
Strand = Plus / Minus
Query: 1416 ccaccacccattccttccttaagggcatcttcaccatactgatcataaatctcacgtttc 1475
||||||||||||||||||||||| ||||| |||||||| ||||||||| ||||| |||
Sbjct: 335 ccaccacccattccttccttaagagcatcctcaccatatgtatcataaatatcacgcttc 276
Query: 1476 tctggatcactcaaaacctcataggcttgtgcgagctccttgaacttctcggggtcaccg 1535
|| |||||||||||||||||||| |||||||| ||||| |||||||||||||| |||||
Sbjct: 275 tcaggatcactcaaaacctcataagcttgtgctagctctttgaacttctcgggatcacca 216
Query: 1536 cccttgtcggggtggttcttgatagc 1561
||||| || || || ||||| |||||
Sbjct: 215 cccttatcaggatgattcttaatagc 190
>gb|AF069507.1|AF069507 Medicago sativa DnaJ-like protein MsJ1 gene, complete cds
Length = 4449
Score = 65.9 bits (33), Expect = 9e-011
Identities = 39/41 (95%)
Strand = Plus / Minus
Query: 1422 cccattccttccttaagggcatcttcaccatactgatcata 1462
||||||||||| |||||||||||||||||||| ||||||||
Sbjct: 1079 cccattccttctttaagggcatcttcaccatattgatcata 1039
Score = 30.2 bits (15), Expect = 4.9
Identities = 27/31 (87%)
Strand = Plus / Minus
Query: 1371 ccaaaaaatgatgagaaaatgtcaaatggat 1401
|||||||||||| |||||| ||||| ||||
Sbjct: 1136 ccaaaaaatgattcgaaaatatcaaacggat 1106
>emb|AJ000995.1|MSDNAJ Medicago sativa mRNA for DnaJ-like protein
Length = 1545
Score = 65.9 bits (33), Expect = 9e-011
Identities = 39/41 (95%)
Strand = Plus / Minus
Query: 1422 cccattccttccttaagggcatcttcaccatactgatcata 1462
||||||||||| |||||||||||||||||||| ||||||||
Sbjct: 308 cccattccttctttaagggcatcttcaccatattgatcata 268
Score = 30.2 bits (15), Expect = 4.9
Identities = 27/31 (87%)
Strand = Plus / Minus
Query: 1371 ccaaaaaatgatgagaaaatgtcaaatggat 1401
|||||||||||| |||||| ||||| ||||
Sbjct: 365 ccaaaaaatgattcgaaaatatcaaacggat 335
>emb|Z71997.1|MSDNAJLP M.sativa mRNA for DnaJ-like protein
Length = 513
Score = 65.9 bits (33), Expect = 9e-011
Identities = 39/41 (95%)
Strand = Plus / Minus
Query: 1422 cccattccttccttaagggcatcttcaccatactgatcata 1462
||||||||||| |||||||||||||||||||| ||||||||
Sbjct: 67 cccattccttctttaagggcatcttcaccatattgatcata 27
>gb|AJ966338.1|AJ966338 AJ966338 Medicago sativa subsp. falcata flower buds Medicago sativa
subsp. falcata cDNA clone Em53, mRNA sequence
Length = 96
Score = 63.9 bits (32), Expect = 4e-010
Identities = 54/60 (90%), Gaps = 1/60 (1%)
Strand = Plus / Minus
Query: 909 acaatgtctccagtaacggtatcaggc-gcttcatcagcttcaccagggaaggtgatctt 967
||||| ||||||||||| || ||||| |||||||| |||||||||||||||||||||||
Sbjct: 64 acaatatctccagtaaccgtgtcaggaagcttcatcggcttcaccagggaaggtgatctt 5
>gb|AJ966532.1|AJ966532 AJ966532 Medicago sativa subsp. falcata flower buds Medicago sativa
subsp. falcata cDNA clone Em52, mRNA sequence
Length = 96
Score = 63.9 bits (32), Expect = 4e-010
Identities = 54/60 (90%), Gaps = 1/60 (1%)
Strand = Plus / Minus
Query: 909 acaatgtctccagtaacg-gtatcaggcgcttcatcagcttcaccagggaaggtgatctt 967
||||| ||||||||||| || ||||| |||||||| |||||||||||||||||||||||
Sbjct: 64 acaatatctccagtaaccagtgtcaggagcttcatcggcttcaccagggaaggtgatctt 5
>gb|AW698418.1|AW698418 G266 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 273
Score = 56.0 bits (28), Expect = 9e-008
Identities = 52/60 (86%)
Strand = Plus / Plus
Query: 638 gtcggggaactccaccgtgaaatgaatgtacagcttccccttcatgaaaggcctctggta 697
||||||||| ||||| || || |||||||| ||||| |||||||| || |||||||||||
Sbjct: 207 gtcggggaattccacagtaaagtgaatgtaaagcttgcccttcataaatggcctctggta 266
Score = 38.2 bits (19), Expect = 0.020
Identities = 28/31 (90%)
Strand = Plus / Plus
Query: 543 tcctcgcattcatctatctccatgtctgtca 573
||||| || ||||| ||||||||||||||||
Sbjct: 112 tcctcacactcatcgatctccatgtctgtca 142
>gb|AW698402.1|AW698402 G249 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 316
Score = 44.1 bits (22), Expect = 3e-004
Identities = 34/38 (89%)
Strand = Plus / Plus
Query: 660 tgaatgtacagcttccccttcatgaaaggcctctggta 697
|||||||| ||||| |||||||| || |||||||||||
Sbjct: 249 tgaatgtaaagcttgcccttcataaatggcctctggta 286
Score = 38.2 bits (19), Expect = 0.020
Identities = 28/31 (90%)
Strand = Plus / Plus
Query: 543 tcctcgcattcatctatctccatgtctgtca 573
||||| || ||||| ||||||||||||||||
Sbjct: 132 tcctcacactcatcgatctccatgtctgtca 162
>gb|CO515218.1|CO515218 s13dSG96A0700053_399301 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 489
Score = 36.2 bits (18), Expect = 0.080
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 1000 cctcaagaaccttcttct 1017
||||||||||||||||||
Sbjct: 201 cctcaagaaccttcttct 218
>gb|AF201458.1|AF201458 Medicago sativa isoflavone reductase-like NAD(P)H-dependent
oxidoreductase (IRL1) mRNA, complete cds
Length = 1220
Score = 34.2 bits (17), Expect = 0.31
Identities = 17/17 (100%)
Strand = Plus / Plus
Query: 1350 ccacctcctccaaaaga 1366
|||||||||||||||||
Sbjct: 533 ccacctcctccaaaaga 549
>gb|M74191.1|ALFESIPB Medicago sativa environmental stress-induced protein mRNA, 3' end
Length = 436
Score = 32.2 bits (16), Expect = 1.2
Identities = 16/16 (100%)
Strand = Plus / Plus
Query: 1370 tccaaaaaatgatgag 1385
||||||||||||||||
Sbjct: 410 tccaaaaaatgatgag 425
>gb|AJ410119.1|AJ410119 AJ410119 Medicago sativa library (Kalo P) Medicago sativa cDNA
clone U393, mRNA sequence
Length = 904
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Minus
Query: 761 tgatttgatgagaag 775
|||||||||||||||
Sbjct: 499 tgatttgatgagaag 485
>gb|CO511943.1|CO511943 s13dSG05H0300032_103786 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 430
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Plus
Query: 1346 tccaccacctcctcc 1360
|||||||||||||||
Sbjct: 31 tccaccacctcctcc 45
>gb|CO513167.1|CO513167 s13dSG23F0700059_129498 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 592
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Plus
Query: 743 aacaacttcaccagg 757
|||||||||||||||
Sbjct: 42 aacaacttcaccagg 56
>gb|CO513484.1|CO513484 s13dSG10G1200088_130132 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 561
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Minus
Query: 761 tgatttgatgagaag 775
|||||||||||||||
Sbjct: 561 tgatttgatgagaag 547
>gb|CO513968.1|CO513968 s13dSG71G0500036_156598 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 247
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Plus
Query: 1311 tcttctcccctcctt 1325
|||||||||||||||
Sbjct: 64 tcttctcccctcctt 78
>gb|CO514716.1|CO514716 s13dSG55C0600050_328092 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 256
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Minus
Query: 1410 gatccaccaccaccc 1424
|||||||||||||||
Sbjct: 17 gatccaccaccaccc 3
>gb|CO514968.1|CO514968 s13dSG63F0600059_397441 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 351
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Plus
Query: 1462 aaatctcacgtttct 1476
|||||||||||||||
Sbjct: 107 aaatctcacgtttct 121
>gb|CO516175.1|CO516175 s13dSG67C0900070_445678 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 554
Score = 30.2 bits (15), Expect = 4.9
Identities = 18/19 (94%)
Strand = Plus / Minus
Query: 267 ttatgttgcagcaaaaaag 285
|||| ||||||||||||||
Sbjct: 207 ttatcttgcagcaaaaaag 189
>gb|CO516573.1|CO516573 s13dSG57G0500037_446474 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 426
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Plus
Query: 1346 tccaccacctcctcc 1360
|||||||||||||||
Sbjct: 31 tccaccacctcctcc 45
>gb|L39960.1|ALFRPE15A Medicago sativa middle repetitive DNA (RPE15) gene, complete cds
Length = 1498
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Minus
Query: 340 cagcaaaaccagaga 354
|||||||||||||||
Sbjct: 305 cagcaaaaccagaga 291
>emb|AJ237729.1|MSA237729 Medicago sativa DNA for putative minuscule inverted repeat
transposable element (MITE), clone D (5' region)
Length = 719
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Minus
Query: 273 tgcagcaaaaaagga 287
|||||||||||||||
Sbjct: 538 tgcagcaaaaaagga 524
>gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase 1 (ifs1) mRNA, partial cds
Length = 1501
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Plus
Query: 1020 tgaacgaccttctca 1034
|||||||||||||||
Sbjct: 367 tgaacgaccttctca 381
>gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase 2 (ifs2) mRNA, partial cds
Length = 1501
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Plus
Query: 1020 tgaacgaccttctca 1034
|||||||||||||||
Sbjct: 367 tgaacgaccttctca 381
>gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase 3 (ifs3) mRNA, partial cds
Length = 1501
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Plus
Query: 1020 tgaacgaccttctca 1034
|||||||||||||||
Sbjct: 367 tgaacgaccttctca 381
>gb|AF439380.1| Medicago sativa retrotransposon polyprotein gene interrupted by LTR
retroelement MCIRE, complete sequence
Length = 18789
Score = 30.2 bits (15), Expect = 4.9
Identities = 15/15 (100%)
Strand = Plus / Plus
Query: 82 tacatgaaaagaaaa 96
|||||||||||||||
Sbjct: 11530 tacatgaaaagaaaa 11544
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: May 2, 2006 2:40 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 3675
Number of Sequences: 7669
Number of extensions: 3675
Number of successful extensions: 1062
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1007
Number of HSP's gapped (non-prelim): 53
length of query: 1723
length of database: 3,745,706
effective HSP length: 16
effective length of query: 1707
effective length of database: 3,623,002
effective search space: 6184464414
effective search space used: 6184464414
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)