BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.202
(3202 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF049487.1|AF049487 Medicago sativa sucrose synthase mRN... 127 5e-029
gb|CO517054.1|CO517054 s13dSG31G0500037_468654 Glandular tr... 94 7e-019
gb|CO514282.1|CO514282 s13dSG76F0500043_157226 Glandular tr... 52 2e-006
gb|DQ122788.1| Medicago sativa clone B11 sucrose synthase m... 52 2e-006
gb|AY302251.1| Medicago sativa beta 1,2 xylosyltransferase ... 36 0.15
gb|DQ122842.1| Medicago sativa clone MF8 sucrose synthase m... 36 0.15
>gb|AF049487.1|AF049487 Medicago sativa sucrose synthase mRNA, complete cds
Length = 2760
Score = 127 bits (64), Expect = 5e-029
Identities = 337/428 (78%)
Strand = Plus / Minus
Query: 1496 tccttgtttccggcgatctcttggaaggtactggtgatgatgaagtcggcatggttcatt 1555
|||||| |||| || ||||||||||||||||| ||||||||||| || | |||||||||
Sbjct: 1527 tccttgcttccagcaatctcttggaaggtactagtgatgatgaaatctgtgtggttcatt 1468
Query: 1556 gcaatcaagtcagtggtgaactggcacgagaagtggtagtgatcctcaaacttcttccag 1615
|| | | |||| ||| || ||||| |||||||| || | || || || || ||||||
Sbjct: 1467 gcgaaaagatcagcggtaaattggcaggagaagtgatacttctcttcgaattttttccag 1408
Query: 1616 tagaggtcggagttagggtacttagttttctcaagcgcatgggcaatggtacagtgagta 1675
|| | ||||| | ||||||||||| ||||| || ||||| ||||||||||| |||||
Sbjct: 1407 taaatatcggattcggggtacttagtcttctcgagtgcatgagcaatggtacactgagtg 1348
Query: 1676 acacccatcttgtgggcgagcaaacacgcaacaaggtttccgtcactgtagtttccgatg 1735
||||| | || || || | |||| | ||||||| |||||| |||||||||||||| |
Sbjct: 1347 acacctaatttatgtgccaacaaagaggcaacaatgtttccatcactgtagtttccaaca 1288
Query: 1736 atcaggtcaggattggcctgaagctctccagcaatctcatgcgccacgtcatcagtgtaa 1795
||||| || || ||| || | |||| |||| |||||| || || || |||||||||
Sbjct: 1287 atcagatctggtttgctttgcaactctttggcaagctcatgagcaacatcctcagtgtaa 1228
Query: 1796 gtctccaggtacggccagacttcaaatcgcgagatccacttgcgaacgattccgttttct 1855
|| || || || ||||||||||||||||| ||||||||||||||||| ||||| || ||
Sbjct: 1227 gtttctagatatggccagacttcaaatcgtgagatccacttgcgaacaattcccttctca 1168
Query: 1856 gttctgaatggcacgcgaaggatatggcagtgctcggtgccaaggaccttctcaaggcgc 1915
|||||| || || ||||| || ||||| ||||| || ||| |||||| ||||| ||
Sbjct: 1167 tctctgaagggaactcgaagaatgtggcaatgctcagttccatagaccttttcaagccgt 1108
Query: 1916 tggccaca 1923
||||||||
Sbjct: 1107 tggccaca 1100
Score = 113 bits (57), Expect = 8e-025
Identities = 99/113 (87%)
Strand = Plus / Minus
Query: 2033 acttgatccaagatgtagacaacctggcctccggtgtcagggtaacccaagacattagct 2092
||||||||||||||||| |||||||||||||| || |||||||| |||||||||| | ||
Sbjct: 990 acttgatccaagatgtaaacaacctggcctccagtatcagggtatcccaagacatcatct 931
Query: 2093 tgagcgaagtaaccatgaggggagaggataacgacattgaacaccatggggat 2145
||||| |||||||||||||| || ||||| || ||||| || ||||| |||||
Sbjct: 930 tgagcaaagtaaccatgaggagaaaggatgacaacattaaagaccattgggat 878
Score = 60.0 bits (30), Expect = 1e-008
Identities = 93/114 (81%)
Strand = Plus / Minus
Query: 913 catggcctcaaccaccgtcagcccgaaagcctcgtagaaagcaggctgcacgaaggcgcc 972
|||||||||||| || |||| |||||||| ||||| | ||||| |||||||| || ||
Sbjct: 2110 catggcctcaacaactgtcaaaccgaaagcttcgtacacggcaggttgcacgaaagctcc 2051
Query: 973 cttggtgtcgcagatgtagcggtacagctcgccgttgcggacgcggttcatctg 1026
||||||||| || || ||||||||||| ||||| | ||| |||||||||||
Sbjct: 2050 cttggtgtcacaaatcacacggtacagctctccgtttctgacacggttcatctg 1997
Score = 52.0 bits (26), Expect = 2e-006
Identities = 107/134 (79%)
Strand = Plus / Minus
Query: 2441 ctctccttgtcatggaagagctttgatgacaggtgcctgttgaggaactgcacgccattg 2500
|||||||||||||||||||| || | || || || | || ||||| ||||| |||||
Sbjct: 582 ctctccttgtcatggaagagtttagcagaaagatggcgattaaggaagtgcacaccattt 523
Query: 2501 ccaatggactttgacagagaaggacgggggaaggaggcattgaatggctcaaagtccagc 2560
||||| ||||| |||| |||||| ||||| || ||| | ||||| |||||||||| |
Sbjct: 522 ccaattgacttgttgagagtaggacgagggaaagatgcagtaaatggttcaaagtccaac 463
Query: 2561 tcaagaacaaagtt 2574
||||| ||||||||
Sbjct: 462 tcaagcacaaagtt 449
Score = 44.1 bits (22), Expect = 6e-004
Identities = 40/46 (86%)
Strand = Plus / Minus
Query: 2250 tctccagaccaagttcctggaacctgtggtgaaattcagagtatgg 2295
|||||| ||||| |||||||||||||| | |||||| ||||||||
Sbjct: 773 tctccaaaccaatctcctggaacctgtgttcaaattctgagtatgg 728
Score = 40.1 bits (20), Expect = 0.010
Identities = 41/48 (85%)
Strand = Plus / Minus
Query: 1210 gttcttcacacggtcgagacgagccatggagaagatgattggcttgtt 1257
||||||||||||||| | | ||||||| |||||| |||||||||||
Sbjct: 1813 gttcttcacacggtccaaccttgccatggtgaagataattggcttgtt 1766
Score = 40.1 bits (20), Expect = 0.010
Identities = 65/80 (81%)
Strand = Plus / Minus
Query: 1355 tcggtgtacgggaagtagatggacaggtccgcgccaggagacacgatgttgaacttgggg 1414
|||||||| |||||||| |||| | | || || || ||||| || ||||||||||| ||
Sbjct: 1668 tcggtgtaagggaagtaaatggtctgatcagctcctggagatacaatgttgaactttgga 1609
Query: 1415 tcgaacacatcaatgccgtg 1434
|| || |||||||| |||||
Sbjct: 1608 tcaaagacatcaataccgtg 1589
Score = 38.2 bits (19), Expect = 0.038
Identities = 46/55 (83%)
Strand = Plus / Minus
Query: 3148 ggagacacgatgttgaacttggggtcgaacacatcaatgccgtggacgacgcggt 3202
||||| || ||||||||||| || || || |||||||| ||||| ||||| ||||
Sbjct: 1632 ggagatacaatgttgaactttggatcaaagacatcaataccgtgcacgacacggt 1578
>gb|CO517054.1|CO517054 s13dSG31G0500037_468654 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 374
Score = 93.7 bits (47), Expect = 7e-019
Identities = 146/179 (81%)
Strand = Plus / Minus
Query: 2009 agcatttcgttctccatagcgcgcacttgatccaagatgtagacaacctggcctccggtg 2068
||||| |||||||||| || || ||||| ||||| ||||| |||||||| || |||||
Sbjct: 227 agcatctcgttctccaaggctcgaacttggtccaaaatgtacacaacctgtccaccggta 168
Query: 2069 tcagggtaacccaagacattagcttgagcgaagtaaccatgaggggagaggataacgaca 2128
|||||||| |||||| ||||| |||| |||||||||||||| || || ||||| || ||
Sbjct: 167 tcagggtatcccaaggcattatcttgggcgaagtaaccatgtggagaaaggatgacagca 108
Query: 2129 ttgaacaccatggggatcgttccaaggaacttctccagggtggacggatctggggcctc 2187
|| || |||| ||||| |||||||||| ||||| |||||| | ||||| || |||||
Sbjct: 107 ttaaaggccatagggattcttccaaggaatttctcgagggtgaaaggatcaggtgcctc 49
>gb|CO514282.1|CO514282 s13dSG76F0500043_157226 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 515
Score = 52.0 bits (26), Expect = 2e-006
Identities = 53/62 (85%)
Strand = Plus / Minus
Query: 911 gtcatggcctcaaccaccgtcagcccgaaagcctcgtagaaagcaggctgcacgaaggcg 970
||||||||||| || ||||| || || ||||| || |||||||||||||| ||||| |||
Sbjct: 413 gtcatggcctctacgaccgtaagtccaaaagcttcatagaaagcaggctgaacgaaagcg 354
Query: 971 cc 972
||
Sbjct: 353 cc 352
>gb|DQ122788.1| Medicago sativa clone B11 sucrose synthase mRNA, partial cds
Length = 536
Score = 52.0 bits (26), Expect = 2e-006
Identities = 53/62 (85%)
Strand = Plus / Minus
Query: 911 gtcatggcctcaaccaccgtcagcccgaaagcctcgtagaaagcaggctgcacgaaggcg 970
||||||||||| || ||||| || || ||||| || |||||||||||||| ||||| |||
Sbjct: 375 gtcatggcctctacgaccgtaagtccaaaagcttcatagaaagcaggctgaacgaaagcg 316
Query: 971 cc 972
||
Sbjct: 315 cc 314
>gb|AY302251.1| Medicago sativa beta 1,2 xylosyltransferase mRNA, complete cds
Length = 1553
Score = 36.2 bits (18), Expect = 0.15
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 2757 cctcaaaagcaccatcct 2774
||||||||||||||||||
Sbjct: 522 cctcaaaagcaccatcct 505
>gb|DQ122842.1| Medicago sativa clone MF8 sucrose synthase mRNA, partial cds
Length = 652
Score = 36.2 bits (18), Expect = 0.15
Identities = 24/26 (92%)
Strand = Plus / Minus
Query: 2666 ctcacatactcccagacaccaggcct 2691
|||| ||||||||| |||||||||||
Sbjct: 483 ctcagatactcccaaacaccaggcct 458
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 6303
Number of Sequences: 7669
Number of extensions: 6303
Number of successful extensions: 1956
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1930
Number of HSP's gapped (non-prelim): 23
length of query: 3202
length of database: 3,745,706
effective HSP length: 17
effective length of query: 3185
effective length of database: 3,615,333
effective search space: 11514835605
effective search space used: 11514835605
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 18 (36.2 bits)