BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QCN28g01.yg.2.1
         (513 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BQ762851.1|BQ762851  EBro02_SQ006_F07_R root, 3 week, hyd...    38   0.27 
gb|CB877438.1|CB877438  HP04O12T HP Hordeum vulgare subsp. v...    38   0.27 

>gb|BQ762851.1|BQ762851 EBro02_SQ006_F07_R root, 3 week, hydroponic grown, low nitrogen, cv
           Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
           EBro02_SQ006_F07 5', mRNA sequence
          Length = 670

 Score = 38.2 bits (19), Expect = 0.27
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 259 caaataaaaaaggatggaa 277
           |||||||||||||||||||
Sbjct: 100 caaataaaaaaggatggaa 118



>gb|CB877438.1|CB877438 HP04O12T HP Hordeum vulgare subsp. vulgare cDNA clone HP04O12
           5-PRIME, mRNA sequence
          Length = 659

 Score = 38.2 bits (19), Expect = 0.27
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 259 caaataaaaaaggatggaa 277
           |||||||||||||||||||
Sbjct: 187 caaataaaaaaggatggaa 205



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 42,854
Number of Sequences: 312970
Number of extensions: 42854
Number of successful extensions: 11357
Number of sequences better than  0.5: 2
Number of HSP's better than  0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 11355
Number of HSP's gapped (non-prelim): 2
length of query: 513
length of database: 175,134,539
effective HSP length: 18
effective length of query: 495
effective length of database: 169,501,079
effective search space: 83903034105
effective search space used: 83903034105
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)