BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCM3d10.yg.2.1
(683 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU998148.1|BU998148 HI10C07r HI Hordeum vulgare subsp. v... 500 e-140
emb|AJ238715.1|HVU238715 Hordeum vulgare partial complement... 159 1e-037
gb|BG369152.1|BG369152 HVSMEi0022M21f Hordeum vulgare 20 DA... 149 1e-034
gb|BJ463533.1|BJ463533 BJ463533 K. Sato unpublished cDNA li... 107 5e-022
gb|CK123660.1|CK123660 BES1824105a02 BES1824 Hordeum vulgar... 107 5e-022
gb|BF619008.2|BF619008 HVSMEc0008G05f Hordeum vulgare seedl... 98 5e-019
gb|CV057680.1|CV057680 BNEL2E7 Barley EST endosperm library... 84 7e-015
gb|AF261107.1|AF261107 Hordeum vulgare sucrose-phosphate sy... 84 7e-015
gb|BU985379.1|BU985379 HF06P13r HF Hordeum vulgare subsp. v... 76 2e-012
gb|BU989121.1|BU989121 HF19N21r HF Hordeum vulgare subsp. v... 68 4e-010
gb|CA007166.1|CA007166 HU06P22r HU Hordeum vulgare subsp. v... 60 1e-007
gb|CA018261.1|CA018261 HV08B18r HV Hordeum vulgare subsp. v... 60 1e-007
gb|BQ468536.1|BQ468536 HM01I14T HM Hordeum vulgare subsp. v... 58 4e-007
>gb|BU998148.1|BU998148 HI10C07r HI Hordeum vulgare subsp. vulgare cDNA clone HI10C07
5-PRIME, mRNA sequence
Length = 614
Score = 500 bits (252), Expect = e-140
Identities = 476/553 (86%), Gaps = 1/553 (0%)
Strand = Plus / Plus
Query: 106 cctcctggcatggagtttagtcatatagcaccacatgatgttgacctcgacagtgaggaa 165
||||| || ||||||||||| | ||||| || ||||||||||| || || ||||| |||
Sbjct: 30 cctcccggtatggagtttagcc-tatagtgcctcatgatgttgatcttgatagtgaagaa 88
Query: 166 ggaaatggagatggctcaggttcaccagatccacctatttgggctgatataatgcgcttc 225
| |||| || | |||||| ||||||||||||||| |||||||||||||||||||||||
Sbjct: 89 gcgaatgaagttagctcagattcaccagatccaccagtttgggctgatataatgcgcttc 148
Query: 226 ttctcaaacccccggaagccaatgattcttgctcttgctcgtccggatccgaagaagaat 285
||||| ||||| || ||||| ||||||||||||||||||||||| ||||| ||||||||
Sbjct: 149 ttctcgaaccctcgcaagcccatgattcttgctcttgctcgtccagatcccaagaagaac 208
Query: 286 atcactactctagtcaaagcatttggtgaacatcgtgaactgagaaatttagcaaatctt 345
|||||||| || || || |||||||||||||| | ||||||||||||||||||||| |||
Sbjct: 209 atcactacgctggttaaggcatttggtgaacaccatgaactgagaaatttagcaaacctt 268
Query: 346 acactgatcatggggaatcgtgatgtcattgatgaaatgtcaagcacaaatgcagctgtt 405
||| |||||||||| || |||||||| ||||||||||||||||||||||||| |||||||
Sbjct: 269 acattgatcatgggtaaccgtgatgttattgatgaaatgtcaagcacaaatggagctgtt 328
Query: 406 ttgacttcagcactcaagttaattgataaatatgatctatatggacaagtggcatacccc 465
||||| |||| ||||||||||||||| || |||||||| ||||| |||||||||||||||
Sbjct: 329 ttgacatcagtactcaagttaattgacaagtatgatctctatgggcaagtggcatacccc 388
Query: 466 aagcaccataagcaatctgaagttcctgatatttatcgtttagctgcgagaacaaaagga 525
|||||||||||||||||||||||||| ||||||||||||||||| || |||||||| ||
Sbjct: 389 aagcaccataagcaatctgaagttccagatatttatcgtttagcggcaagaacaaagggg 448
Query: 526 gtttttatcaattgtgcattggttgaaccatttggactcaccttgattgaggntnnngca 585
|| ||||| || ||||| | ||||||||||||| ||||||||||| |||| | ||
Sbjct: 449 gtgtttattaactgtgcttatattgaaccatttgggctcaccttgatcgaggctgctgct 508
Query: 586 tatggtctacccatgnnnnncacccgaaatggtgggcctgtnnacatacatcgggttctt 645
||||||||||| ||| |||| ||||||||||||||| | ||||| |||||||||
Sbjct: 509 tatggtctacctatggttgctacccaaaatggtgggcctgttgatatacaccgggttctt 568
Query: 646 gataatggaattc 658
|| ||||| ||||
Sbjct: 569 gacaatggcattc 581
>emb|AJ238715.1|HVU238715 Hordeum vulgare partial complement codded sucrose-phosphate
synthase 1 (235 bp)
Length = 235
Score = 159 bits (80), Expect = 1e-037
Identities = 157/183 (85%)
Strand = Plus / Minus
Query: 93 tatgattgcaatccctcctggcatggagtttagtcatatagcaccacatgatgttgacct 152
||||||| |||| ||||| || ||||||||||| ||||||| || ||||||||||| ||
Sbjct: 197 tatgattccaattcctcccggtatggagtttagccatatagtgcctcatgatgttgatct 138
Query: 153 cgacagtgaggaaggaaatggagatggctcaggttcaccagatccacctatttgggctga 212
|| ||||| |||| |||| || | |||||| ||||||||||||||| ||||||||||
Sbjct: 137 tgatagtgaagaagcgaatgaagttagctcagattcaccagatccaccagtttgggctga 78
Query: 213 tataatgcgcttcttctcaaacccccggaagccaatgattcttgctcttgctcgtccgga 272
|||||||||||| ||||| ||||| || ||||| ||||||||||||||||||||||| ||
Sbjct: 77 tataatgcgcttnttctcgaaccctcgcaagcccatgattcttgctcttgctcgtccaga 18
Query: 273 tcc 275
|||
Sbjct: 17 tcc 15
>gb|BG369152.1|BG369152 HVSMEi0022M21f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0022M21f, mRNA sequence
Length = 826
Score = 149 bits (75), Expect = 1e-034
Identities = 189/230 (82%)
Strand = Plus / Plus
Query: 35 ggaaactcagagcaagaataaggcgtggtgtgagctgctttggtcgtnanannnnnnnta 94
||||||| ||||||||||||| ||||||||| ||||||| |||||| | | ||
Sbjct: 281 ggaaacttagagcaagaataaagcgtggtgttagctgctacggtcgtgaaatgcctcgta 340
Query: 95 tgattgcaatccctcctggcatggagtttagtcatatagcaccacatgatgttgacctcg 154
||||| |||| ||||| || ||||||||||| ||||||| || ||||||||||| || |
Sbjct: 341 tgattccaattcctcccggtatggagtttagccatatagtgcctcatgatgttgatcttg 400
Query: 155 acagtgaggaaggaaatggagatggctcaggttcaccagatccacctatttgggctgata 214
| ||||| |||| |||| || | |||||| |||||||||||||||| ||||||| ||||
Sbjct: 401 atagtgaagaagctaatgaagttagctcagattcaccagatccacctgtttgggccgata 460
Query: 215 taatgcgcttcttctcaaacccccggaagccaatgattcttgctcttgct 264
||||||||||||||| | |||| | ||||| |||||||||||| |||||
Sbjct: 461 taatgcgcttcttctgagaccctcacaagcccatgattcttgctgttgct 510
>gb|BJ463533.1|BJ463533 BJ463533 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32g04 5', mRNA sequence
Length = 705
Score = 107 bits (54), Expect = 5e-022
Identities = 108/126 (85%)
Strand = Plus / Plus
Query: 396 tgcagctgttttgacttcagcactcaagttaattgataaatatgatctatatggacaagt 455
||||||||||||||| |||| ||| | || |||||| |||||||| | ||||| |||||
Sbjct: 363 tgcagctgttttgacatcagtacttacattgattgatgaatatgatttgtatggtcaagt 422
Query: 456 ggcataccccaagcaccataagcaatctgaagttcctgatatttatcgtttagctgcgag 515
||||||||| ||||| || || || || ||||||| |||||||||||||||||| |||||
Sbjct: 423 ggcatacccaaagcatcacaaacactcagaagttcttgatatttatcgtttagcagcgag 482
Query: 516 aacaaa 521
||||||
Sbjct: 483 aacaaa 488
Score = 60.0 bits (30), Expect = 1e-007
Identities = 66/78 (84%)
Strand = Plus / Plus
Query: 192 agatccacctatttgggctgatataatgcgcttcttctcaaacccccggaagccaatgat 251
|||||||||||||||| |||| |||||||| |||||| |||| || |||| || ||||
Sbjct: 159 agatccacctatttggtctgagataatgcggttcttcacaaatcctaggaaacctttgat 218
Query: 252 tcttgctcttgctcgtcc 269
||| ||| ||||||||||
Sbjct: 219 tctggctgttgctcgtcc 236
>gb|CK123660.1|CK123660 BES1824105a02 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010A025 5-PRIME, mRNA sequence
Length = 759
Score = 107 bits (54), Expect = 5e-022
Identities = 108/126 (85%)
Strand = Plus / Plus
Query: 396 tgcagctgttttgacttcagcactcaagttaattgataaatatgatctatatggacaagt 455
||||||||||||||| |||| ||| | || |||||| |||||||| | ||||| |||||
Sbjct: 63 tgcagctgttttgacatcagtacttacattgattgatgaatatgatttgtatggtcaagt 122
Query: 456 ggcataccccaagcaccataagcaatctgaagttcctgatatttatcgtttagctgcgag 515
||||||||| ||||| || || || || ||||||| |||||||||||||||||| |||||
Sbjct: 123 ggcatacccaaagcatcacaaacactcagaagttcttgatatttatcgtttagcagcgag 182
Query: 516 aacaaa 521
||||||
Sbjct: 183 aacaaa 188
>gb|BF619008.2|BF619008 HVSMEc0008G05f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0008G05f, mRNA sequence
Length = 590
Score = 97.6 bits (49), Expect = 5e-019
Identities = 106/125 (84%)
Strand = Plus / Plus
Query: 396 tgcagctgttttgacttcagcactcaagttaattgataaatatgatctatatggacaagt 455
||||||||||||||| |||| ||| | || |||||| |||||||| | ||||| |||||
Sbjct: 209 tgcagctgttttgacatcagtacttacattgattgatgaatatgatttgtatggtcaagt 268
Query: 456 ggcataccccaagcaccataagcaatctgaagttcctgatatttatcgtttagctgcgag 515
||||||||| ||||| || || || || ||||||| |||||||||||||||||| ||||
Sbjct: 269 ggcatacccaaagcatcacaaacactcagaagttcttgatatttatcgtttagcagcgaa 328
Query: 516 aacaa 520
|||||
Sbjct: 329 aacaa 333
Score = 52.0 bits (26), Expect = 2e-005
Identities = 38/42 (90%)
Strand = Plus / Plus
Query: 192 agatccacctatttgggctgatataatgcgcttcttctcaaa 233
|||||||||||||||| |||| |||||||| |||||| ||||
Sbjct: 4 agatccacctatttggtctgagataatgcggttcttcacaaa 45
>gb|CV057680.1|CV057680 BNEL2E7 Barley EST endosperm library Hordeum vulgare subsp. vulgare
cDNA clone BNEL2E7 5' similar to sucrose-phosphate
synthase, mRNA sequence
Length = 700
Score = 83.8 bits (42), Expect = 7e-015
Identities = 66/74 (89%)
Strand = Plus / Plus
Query: 436 tatgatctatatggacaagtggcataccccaagcaccataagcaatctgaagttcctgat 495
|||||| | ||||| |||||||||||||||||||| || ||||| ||||||||||||||
Sbjct: 499 tatgatttgtatggtcaagtggcataccccaagcatcacaagcactctgaagttcctgac 558
Query: 496 atttatcgtttagc 509
||||| ||||||||
Sbjct: 559 atttaccgtttagc 572
Score = 56.0 bits (28), Expect = 2e-006
Identities = 100/124 (80%)
Strand = Plus / Plus
Query: 192 agatccacctatttgggctgatataatgcgcttcttctcaaacccccggaagccaatgat 251
|||||| ||||| ||| || | |||||||||||||| |||| || |||||||| |||||
Sbjct: 255 agatccgcctatctggtctcagataatgcgcttctttacaaatcctcggaagcctatgat 314
Query: 252 tcttgctcttgctcgtccggatccgaagaagaatatcactactctagtcaaagcatttgg 311
||| ||| |||| ||||| |||| | |||||||| || || || || || ||||||||
Sbjct: 315 tctggctgttgcccgtccatatcctgaaaagaatataacaacgcttgtaaaggcatttgg 374
Query: 312 tgaa 315
||||
Sbjct: 375 tgaa 378
>gb|AF261107.1|AF261107 Hordeum vulgare sucrose-phosphate synthase mRNA, partial cds
Length = 2085
Score = 83.8 bits (42), Expect = 7e-015
Identities = 66/74 (89%)
Strand = Plus / Plus
Query: 436 tatgatctatatggacaagtggcataccccaagcaccataagcaatctgaagttcctgat 495
|||||| | ||||| |||||||||||||||||||| || ||||| ||||||||||||||
Sbjct: 505 tatgatttgtatggtcaagtggcataccccaagcatcacaagcactctgaagttcctgac 564
Query: 496 atttatcgtttagc 509
||||| ||||||||
Sbjct: 565 atttaccgtttagc 578
Score = 56.0 bits (28), Expect = 2e-006
Identities = 100/124 (80%)
Strand = Plus / Plus
Query: 192 agatccacctatttgggctgatataatgcgcttcttctcaaacccccggaagccaatgat 251
|||||| ||||| ||| || | |||||||||||||| |||| || |||||||| |||||
Sbjct: 261 agatccgcctatctggtctcagataatgcgcttctttacaaatcctcggaagcctatgat 320
Query: 252 tcttgctcttgctcgtccggatccgaagaagaatatcactactctagtcaaagcatttgg 311
||| ||| |||| ||||| |||| | |||||||| || || || || || ||||||||
Sbjct: 321 tctggctgttgcccgtccatatcctgaaaagaatataacaacgcttgtaaaggcatttgg 380
Query: 312 tgaa 315
||||
Sbjct: 381 tgaa 384
>gb|BU985379.1|BU985379 HF06P13r HF Hordeum vulgare subsp. vulgare cDNA clone HF06P13
5-PRIME, mRNA sequence
Length = 559
Score = 75.8 bits (38), Expect = 2e-012
Identities = 90/111 (81%)
Strand = Plus / Plus
Query: 548 ttgaaccatttggactcaccttgattgaggntnnngcatatggtctacccatgnnnnnca 607
||||||||||||| ||||||||||| |||| | || ||||||||||| ||| |
Sbjct: 12 ttgaaccatttgggctcaccttgatcgaggctgctgcttatggtctacctatggttgcta 71
Query: 608 cccgaaatggtgggcctgtnnacatacatcgggttcttgataatggaattc 658
||| ||||||||||||||| | ||||| ||||||||||| ||||| ||||
Sbjct: 72 cccaaaatggtgggcctgttgatatacaccgggttcttgacaatggcattc 122
>gb|BU989121.1|BU989121 HF19N21r HF Hordeum vulgare subsp. vulgare cDNA clone HF19N21
5-PRIME, mRNA sequence
Length = 499
Score = 67.9 bits (34), Expect = 4e-010
Identities = 85/102 (83%)
Strand = Plus / Plus
Query: 396 tgcagctgttttgacttcagcactcaagttaattgataaatatgatctatatggacaagt 455
||||||||||||||| |||| ||| | || |||||| |||||||| | ||||| |||||
Sbjct: 398 tgcagctgttttgacatcagtacttacattgattgatgaatatgatttgtatggtcaagt 457
Query: 456 ggcataccccaagcaccataagcaatctgaagttcctgatat 497
||||||||| ||||| || || || || ||||||| ||||||
Sbjct: 458 ggcatacccaaagcatcacaaacactcagaagttcttgatat 499
>gb|CA007166.1|CA007166 HU06P22r HU Hordeum vulgare subsp. vulgare cDNA clone HU06P22
5-PRIME, mRNA sequence
Length = 660
Score = 60.0 bits (30), Expect = 1e-007
Identities = 66/78 (84%)
Strand = Plus / Plus
Query: 192 agatccacctatttgggctgatataatgcgcttcttctcaaacccccggaagccaatgat 251
|||||||||||||||| |||| |||||||| |||||| |||| || |||| || ||||
Sbjct: 413 agatccacctatttggtctgagataatgcggttcttcacaaatcctaggaaacctttgat 472
Query: 252 tcttgctcttgctcgtcc 269
||| ||| ||||||||||
Sbjct: 473 tctggctgttgctcgtcc 490
>gb|CA018261.1|CA018261 HV08B18r HV Hordeum vulgare subsp. vulgare cDNA clone HV08B18
5-PRIME, mRNA sequence
Length = 634
Score = 60.0 bits (30), Expect = 1e-007
Identities = 66/78 (84%)
Strand = Plus / Plus
Query: 192 agatccacctatttgggctgatataatgcgcttcttctcaaacccccggaagccaatgat 251
|||||||||||||||| |||| |||||||| |||||| |||| || |||| || ||||
Sbjct: 387 agatccacctatttggtctgagataatgcggttcttcacaaatcctaggaaacctttgat 446
Query: 252 tcttgctcttgctcgtcc 269
||| ||| ||||||||||
Sbjct: 447 tctggctgttgctcgtcc 464
>gb|BQ468536.1|BQ468536 HM01I14T HM Hordeum vulgare subsp. vulgare cDNA clone HM01I14
5-PRIME, mRNA sequence
Length = 575
Score = 58.0 bits (29), Expect = 4e-007
Identities = 38/41 (92%)
Strand = Plus / Plus
Query: 271 gatccgaagaagaatatcactactctagtcaaagcatttgg 311
|||||||| ||||| ||||||||||| ||||||||||||||
Sbjct: 132 gatccgaaaaagaacatcactactcttgtcaaagcatttgg 172
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 51,601
Number of Sequences: 312970
Number of extensions: 51601
Number of successful extensions: 13606
Number of sequences better than 0.5: 13
Number of HSP's better than 0.5 without gapping: 13
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 13575
Number of HSP's gapped (non-prelim): 28
length of query: 683
length of database: 175,134,539
effective HSP length: 19
effective length of query: 664
effective length of database: 169,188,109
effective search space: 112340904376
effective search space used: 112340904376
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)