BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QCM12h10.yg.2.1
         (339 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BU971000.1|BU971000  HB16F02r BC Hordeum vulgare subsp. v...   101   1e-020
gb|BE559011.2|BE559011  HV_CEb0022E12f Hordeum vulgare seedl...    54   3e-006

>gb|BU971000.1|BU971000 HB16F02r BC Hordeum vulgare subsp. vulgare cDNA clone HB16F02
           5-PRIME, mRNA sequence
          Length = 380

 Score =  101 bits (51), Expect = 1e-020
 Identities = 83/95 (87%)
 Strand = Plus / Minus

                                                                       
Query: 185 cctagcatacagcntgtaattttcagcaaaactcttgtgctgcacaacaaaaccacgnnn 244
           ||||||||| ||| ||||||||||||| |||||||| |||||||||||||| |||||   
Sbjct: 380 cctagcatatagcctgtaattttcagcgaaactcttatgctgcacaacaaagccacggcc 321

                                              
Query: 245 agtagcacggtattttgcaccaccatgaaggattg 279
           |||||| || || ||||||||||||||||| ||||
Sbjct: 320 agtagcccgatactttgcaccaccatgaagaattg 286



>gb|BE559011.2|BE559011 HV_CEb0022E12f Hordeum vulgare seedling green leaf EST library
           HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
           vulgare cDNA clone HV_CEb0022E12f, mRNA sequence
          Length = 730

 Score = 54.0 bits (27), Expect = 3e-006
 Identities = 45/52 (86%)
 Strand = Plus / Minus

                                                               
Query: 228 acaacaaaaccacgnnnagtagcacggtattttgcaccaccatgaaggattg 279
           ||||||||||| ||   ||||||||| |  ||||||||||||||||||||||
Sbjct: 213 acaacaaaacctcgtccagtagcacgatgctttgcaccaccatgaaggattg 162



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 18,851
Number of Sequences: 312970
Number of extensions: 18851
Number of successful extensions: 4664
Number of sequences better than  0.5: 2
Number of HSP's better than  0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 4661
Number of HSP's gapped (non-prelim): 3
length of query: 339
length of database: 175,134,539
effective HSP length: 18
effective length of query: 321
effective length of database: 169,501,079
effective search space: 54409846359
effective search space used: 54409846359
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)