BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCM12g02.yg.2.1
(480 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU971000.1|BU971000 HB16F02r BC Hordeum vulgare subsp. v... 313 3e-084
gb|BE559011.2|BE559011 HV_CEb0022E12f Hordeum vulgare seedl... 66 1e-009
gb|BJ462458.1|BJ462458 BJ462458 K. Sato unpublished cDNA li... 54 4e-006
>gb|BU971000.1|BU971000 HB16F02r BC Hordeum vulgare subsp. vulgare cDNA clone HB16F02
5-PRIME, mRNA sequence
Length = 380
Score = 313 bits (158), Expect = 3e-084
Identities = 242/270 (89%)
Strand = Plus / Minus
Query: 185 cctagcatacagcctgtaattttcagcaaaactcttgtgctgcacaacaaaaccacggcc 244
||||||||| ||||||||||||||||| |||||||| |||||||||||||| ||||||||
Sbjct: 380 cctagcatatagcctgtaattttcagcgaaactcttatgctgcacaacaaagccacggcc 321
Query: 245 agtagcacggtattttgcaccaccatgaaggattgtccggccatagtaatggctcttagt 304
|||||| || || ||||||||||||||||| ||||| ||||||||||||||||| || ||
Sbjct: 320 agtagcccgatactttgcaccaccatgaagaattgttcggccatagtaatggctttttgt 261
Query: 305 tcccatggaaaatgtgtagaacacagatgaaaaattcatttgcattgtgaagaaatccca 364
|||||| |||||||||||||| ||||||||||| ||||| ||||||||||| ||||||||
Sbjct: 260 tcccattgaaaatgtgtagaagacagatgaaaagttcatctgcattgtgaaaaaatccca 201
Query: 365 gatagcaggtaggaaaccttgttcaagagagttctctataatcattggcaatgcagtgaa 424
|| |||||||| ||||| |||||||||| ||||||| ||||||||||| ||||||||
Sbjct: 200 gacagcaggtaaaaaacccagttcaagagaattctctaaaatcattggcagggcagtgaa 141
Query: 425 gaagccaagctgtatgatgaactgctgatt 454
|||||| |||||||||| |||||||||||
Sbjct: 140 gaagccgagctgtatgacaaactgctgatt 111
>gb|BE559011.2|BE559011 HV_CEb0022E12f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0022E12f, mRNA sequence
Length = 730
Score = 65.9 bits (33), Expect = 1e-009
Identities = 48/53 (90%)
Strand = Plus / Minus
Query: 228 acaacaaaaccacggccagtagcacggtattttgcaccaccatgaaggattgt 280
||||||||||| || ||||||||||| | |||||||||||||||||||||||
Sbjct: 213 acaacaaaacctcgtccagtagcacgatgctttgcaccaccatgaaggattgt 161
>gb|BJ462458.1|BJ462458 BJ462458 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags28g06 5', mRNA sequence
Length = 666
Score = 54.0 bits (27), Expect = 4e-006
Identities = 90/111 (81%)
Strand = Plus / Minus
Query: 344 ttgcattgtgaagaaatcccagatagcaggtaggaaaccttgttcaagagagttctctat 403
||||||| | ||||||||||| | |||| ||||| || || ||||| || || || ||
Sbjct: 650 ttgcatttttaagaaatcccatacagcattgaggaagccatgctcaagtgaattttcaat 591
Query: 404 aatcattggcaatgcagtgaagaagccaagctgtatgatgaactgctgatt 454
|||||| |||||||| ||||| | ||| |||||||||| |||||||||||
Sbjct: 590 aatcatgggcaatgctgtgaacaggcccagctgtatgacaaactgctgatt 540
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 36,202
Number of Sequences: 312970
Number of extensions: 36202
Number of successful extensions: 9184
Number of sequences better than 0.5: 3
Number of HSP's better than 0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 9181
Number of HSP's gapped (non-prelim): 3
length of query: 480
length of database: 175,134,539
effective HSP length: 18
effective length of query: 462
effective length of database: 169,501,079
effective search space: 78309498498
effective search space used: 78309498498
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)