BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCG22g05.yg.2.1
(681 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF260175.2|BF260175 HVSMEf0021E22f Hordeum vulgare seedl... 398 e-110
gb|AV914570.1|AV914570 AV914570 K. Sato unpublished cDNA li... 117 5e-025
gb|BM371382.2|BM371382 EBma08_SQ002_I06_R maternal, 28 DPA,... 46 0.001
gb|BM928843.1|BM928843 LP10030 Lemma/palea-enriched cDNA li... 42 0.023
gb|BQ134670.1|BQ134670 LP30108 Lemma/palea-enriched cDNA li... 42 0.023
gb|BM376094.2|BM376094 EBma01_SQ002_F09_R maternal, 4 DPA, ... 42 0.023
gb|BM371381.2|BM371381 EBma08_SQ002_I05_R maternal, 28 DPA,... 42 0.023
gb|BM376133.1|BM376133 EBma01_SQ002_H07_R maternal, 4 DPA, ... 40 0.092
gb|BM928833.1|BM928833 LP10006 Lemma/palea-enriched cDNA li... 40 0.092
gb|BM928834.1|BM928834 LP10007 Lemma/palea-enriched cDNA li... 40 0.092
gb|BM928858.1|BM928858 LP20059 Lemma/palea-enriched cDNA li... 40 0.092
gb|BQ134683.1|BQ134683 LP30159 Lemma/palea-enriched cDNA li... 40 0.092
gb|BM376088.2|BM376088 EBma01_SQ002_F01_R maternal, 4 DPA, ... 40 0.092
gb|BM371429.2|BM371429 EBma08_SQ002_L04_R maternal, 28 DPA,... 40 0.092
gb|CD240764.1|CD240764 LP30021 Lemma/palea-enriched cDNA li... 40 0.092
gb|BE215635.1|BE215635 HV_CEb0007H14f Hordeum vulgare seedl... 38 0.37
gb|BM928867.1|BM928867 LP300020 Lemma/palea-enriched cDNA l... 38 0.37
gb|CK123771.1|CK123771 BES1824105f02 BES1824 Hordeum vulgar... 38 0.37
>gb|BF260175.2|BF260175 HVSMEf0021E22f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0021E22f, mRNA sequence
Length = 792
Score = 398 bits (201), Expect = e-110
Identities = 380/439 (86%), Gaps = 3/439 (0%)
Strand = Plus / Minus
Query: 17 gtactctgggtccaggtatccaactgtccctcgaactatacttgacacatgagacccatc 76
||||||||| || || || ||||| || || |||||||| ||||| ||||||||||||||
Sbjct: 439 gtactctggatcaagatacccaaccgttccacgaactatgcttgatacatgagacccatc 380
Query: 77 cactgcaggtttcgaaagcccaaagtctgccacttttgctctcatgttcttgtctaggag 136
|| |||||||| || || |||||||||||||||||||| |||||||||||||| || |
Sbjct: 379 gaccgcaggttttgataggccaaagtctgccacttttgccctcatgttcttgtcaagtaa 320
Query: 137 aatgttactgctcttcaggtctctatggatgattgttggggagcatcccgtgtggagata 196
||||||||| ||||| | |||||| || || || ||||||||||||||||||||||| ||
Sbjct: 319 aatgttactactcttaacgtctctgtgaattatggttggggagcatcccgtgtggaggta 260
Query: 197 ctctatacctttcgcagaatcttctgcaatctcaagacgcttcagccagctagttatctt 256
||||||||||||||||| ||||||||| |||||||| ||||| | |||||||||||| ||
Sbjct: 259 ctctatacctttcgcagcatcttctgctatctcaaggcgcttaacccagctagttatttt 200
Query: 257 ttcattatcag---ctccacgaaggtgctcttttagtgtcccattatgcatgaactcata 313
||| || || ||||||||||||||||||||||||| ||||| ||||||||||||||
Sbjct: 199 cacatcattaggtcctccacgaaggtgctcttttagtgttccattgtgcatgaactcata 140
Query: 314 cactagtatgtttttgccatcttgctgactgtaaccaaggaatgtaaccaggtgtctatg 373
||| |||||||| || || ||||||||||||||||||||||| || |||| || |||||
Sbjct: 139 cacaagtatgttcttcccgtcttgctgactgtaaccaaggaaggttaccaagttcctatg 80
Query: 374 atgtattctggaaagcaatgtcacctcattcaagaattctctgattccctgatacgagtc 433
||||||||| |||||||| | ||||||||||||||||| || ||| |||||||| |||||
Sbjct: 79 atgtattctcgaaagcaacgccacctcattcaagaattgtcggatgccctgatatgagtc 20
Query: 434 attcgtgagaagtttgact 452
||| |||||||| ||||||
Sbjct: 19 atttgtgagaagcttgact 1
>gb|AV914570.1|AV914570 AV914570 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags7a01 5', mRNA sequence
Length = 501
Score = 117 bits (59), Expect = 5e-025
Identities = 116/135 (85%)
Strand = Plus / Minus
Query: 17 gtactctgggtccaggtatccaactgtccctcgaactatacttgacacatgagacccatc 76
||||||||| || || || ||||| || || |||||||| ||||| ||||||||||||||
Sbjct: 140 gtactctggatcaagatacccaaccgttccacgaactatgcttgatacatgagacccatc 81
Query: 77 cactgcaggtttcgaaagcccaaagtctgccacttttgctctcatgttcttgtctaggag 136
|| |||||||| || || |||||||||||||||||||| |||||||||||||| || |
Sbjct: 80 gaccgcaggttttgataggccaaagtctgccacttttgccctcatgttcttgtcaagtaa 21
Query: 137 aatgttactgctctt 151
||||||||| |||||
Sbjct: 20 aatgttactactctt 6
>gb|BM371382.2|BM371382 EBma08_SQ002_I06_R maternal, 28 DPA, no treatment, cv Optic, EBma08
Hordeum vulgare subsp. vulgare cDNA clone
EBma08_SQ002_I06 5', mRNA sequence
Length = 494
Score = 46.1 bits (23), Expect = 0.001
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtactct 23
|||||||||||||||||||||||
Sbjct: 433 gcggccgcccgggcaggtactct 411
>gb|BM928843.1|BM928843 LP10030 Lemma/palea-enriched cDNA library from elongation stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 1-30
similar to Cytosolic GA3PDH gene, mRNA sequence
Length = 478
Score = 42.1 bits (21), Expect = 0.023
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtact 21
|||||||||||||||||||||
Sbjct: 290 gcggccgcccgggcaggtact 270
Score = 40.1 bits (20), Expect = 0.092
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 283 gcggccgcccgggcaggtac 302
>gb|BQ134670.1|BQ134670 LP30108 Lemma/palea-enriched cDNA library from dough stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 3-108,
mRNA sequence
Length = 530
Score = 42.1 bits (21), Expect = 0.023
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtact 21
|||||||||||||||||||||
Sbjct: 347 gcggccgcccgggcaggtact 327
>gb|BM376094.2|BM376094 EBma01_SQ002_F09_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ002_F09 5', mRNA sequence
Length = 387
Score = 42.1 bits (21), Expect = 0.023
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtact 21
|||||||||||||||||||||
Sbjct: 353 gcggccgcccgggcaggtact 333
>gb|BM371381.2|BM371381 EBma08_SQ002_I05_R maternal, 28 DPA, no treatment, cv Optic, EBma08
Hordeum vulgare subsp. vulgare cDNA clone
EBma08_SQ002_I05 5', mRNA sequence
Length = 581
Score = 42.1 bits (21), Expect = 0.023
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtact 21
|||||||||||||||||||||
Sbjct: 519 gcggccgcccgggcaggtact 499
>gb|BM376133.1|BM376133 EBma01_SQ002_H07_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ002_H07 5', mRNA sequence
Length = 422
Score = 40.1 bits (20), Expect = 0.092
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 360 gcggccgcccgggcaggtac 341
>gb|BM928833.1|BM928833 LP10006 Lemma/palea-enriched cDNA library from elongation stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 1-6,
mRNA sequence
Length = 544
Score = 40.1 bits (20), Expect = 0.092
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 254 gcggccgcccgggcaggtac 273
Score = 40.1 bits (20), Expect = 0.092
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 261 gcggccgcccgggcaggtac 242
>gb|BM928834.1|BM928834 LP10007 Lemma/palea-enriched cDNA library from elongation stage
of kernel Hordeum vulgare subsp. vulgare cDNA clone
1-7, mRNA sequence
Length = 420
Score = 40.1 bits (20), Expect = 0.092
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 10 gcggccgcccgggcaggtac 29
>gb|BM928858.1|BM928858 LP20059 Lemma/palea-enriched cDNA library from gelatinous stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 2-59
similar to Rubisco small sub-unit gene, mRNA sequence
Length = 491
Score = 40.1 bits (20), Expect = 0.092
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 260 gcggccgcccgggcaggtac 241
>gb|BQ134683.1|BQ134683 LP30159 Lemma/palea-enriched cDNA library from dough stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 3-159
similar to putative ABC transporter, mRNA sequence
Length = 339
Score = 40.1 bits (20), Expect = 0.092
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 281 gcggccgcccgggcaggtac 262
>gb|BM376088.2|BM376088 EBma01_SQ002_F01_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ002_F01 5', mRNA sequence
Length = 256
Score = 40.1 bits (20), Expect = 0.092
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 84 gcggccgcccgggcaggtac 103
>gb|BM371429.2|BM371429 EBma08_SQ002_L04_R maternal, 28 DPA, no treatment, cv Optic, EBma08
Hordeum vulgare subsp. vulgare cDNA clone
EBma08_SQ002_L04 5', mRNA sequence
Length = 454
Score = 40.1 bits (20), Expect = 0.092
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 84 gcggccgcccgggcaggtac 103
>gb|CD240764.1|CD240764 LP30021 Lemma/palea-enriched cDNA library from dough stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 3-21
similar to A. thaliana AtRer1A, Accession No. AY044321,
mRNA sequence
Length = 342
Score = 40.1 bits (20), Expect = 0.092
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 1 gcggccgcccgggcaggtac 20
||||||||||||||||||||
Sbjct: 323 gcggccgcccgggcaggtac 342
Score = 38.2 bits (19), Expect = 0.37
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 1 gcggccgcccgggcaggta 19
|||||||||||||||||||
Sbjct: 330 gcggccgcccgggcaggta 312
>gb|BE215635.1|BE215635 HV_CEb0007H14f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0007H14f, mRNA sequence
Length = 533
Score = 38.2 bits (19), Expect = 0.37
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 301 gcatgaactcatacactag 319
|||||||||||||||||||
Sbjct: 438 gcatgaactcatacactag 420
>gb|BM928867.1|BM928867 LP300020 Lemma/palea-enriched cDNA library from dough stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 3-20,
mRNA sequence
Length = 216
Score = 38.2 bits (19), Expect = 0.37
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 2 cggccgcccgggcaggtac 20
|||||||||||||||||||
Sbjct: 212 cggccgcccgggcaggtac 194
>gb|CK123771.1|CK123771 BES1824105f02 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010F025 5-PRIME, mRNA sequence
Length = 808
Score = 38.2 bits (19), Expect = 0.37
Identities = 25/27 (92%)
Strand = Plus / Minus
Query: 125 cttgtctaggagaatgttactgctctt 151
|||||| | ||||||||||||||||||
Sbjct: 270 cttgtccaagagaatgttactgctctt 244
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 38,708
Number of Sequences: 312970
Number of extensions: 38708
Number of successful extensions: 12217
Number of sequences better than 0.5: 18
Number of HSP's better than 0.5 without gapping: 18
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 12190
Number of HSP's gapped (non-prelim): 27
length of query: 681
length of database: 175,134,539
effective HSP length: 19
effective length of query: 662
effective length of database: 169,188,109
effective search space: 112002528158
effective search space used: 112002528158
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)