BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCA17a05.yg.2.1
(548 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV915078.1|AV915078 AV915078 K. Sato unpublished cDNA li... 176 5e-043
gb|AV915881.1|AV915881 AV915881 K. Sato unpublished cDNA li... 176 5e-043
gb|AV920212.1|AV920212 AV920212 K. Sato unpublished cDNA li... 176 5e-043
gb|CB878649.1|CB878649 HP09F01T HP Hordeum vulgare subsp. v... 127 4e-028
gb|AV920993.1|AV920993 AV920993 K. Sato unpublished cDNA li... 103 6e-021
gb|AV833885.1|AV833885 AV833885 K. Sato unpublished cDNA li... 58 3e-007
gb|BJ467229.1|BJ467229 BJ467229 K. Sato unpublished cDNA li... 38 0.29
>gb|AV915078.1|AV915078 AV915078 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags9m07 5', mRNA sequence
Length = 623
Score = 176 bits (89), Expect = 5e-043
Identities = 110/117 (94%)
Strand = Plus / Minus
Query: 314 gacagcttctcccagaggcacagcatctgcctgggggactggtagtgcgccgtgaagtag 373
||||| || |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 253 gacagtttgtcccagaggcacagcatctgcctgggggactggtagtgcgccgtgaagtag 194
Query: 374 ttgtccacgcagccgaactggcagaacctccagctgtggacatactgcacgggggcg 430
||||||||||||||| ||||||||||| |||||||||||| |||||||| ||||||
Sbjct: 193 ttgtccacgcagccgtactggcagaacttccagctgtggatgtactgcaccggggcg 137
Score = 81.8 bits (41), Expect = 2e-014
Identities = 50/53 (94%)
Strand = Plus / Minus
Query: 436 gttgtagtcctccacccacatgcccatgctcacgtcctccatcttgaacagcc 488
||||||||| || |||||||||||||||||||| |||||||||||||||||||
Sbjct: 122 gttgtagtcttcaacccacatgcccatgctcacatcctccatcttgaacagcc 70
>gb|AV915881.1|AV915881 AV915881 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16g24 5', mRNA sequence
Length = 606
Score = 176 bits (89), Expect = 5e-043
Identities = 110/117 (94%)
Strand = Plus / Minus
Query: 314 gacagcttctcccagaggcacagcatctgcctgggggactggtagtgcgccgtgaagtag 373
||||| || |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 250 gacagtttgtcccagaggcacagcatctgcctgggggactggtagtgcgccgtgaagtag 191
Query: 374 ttgtccacgcagccgaactggcagaacctccagctgtggacatactgcacgggggcg 430
||||||||||||||| ||||||||||| |||||||||||| |||||||| ||||||
Sbjct: 190 ttgtccacgcagccgtactggcagaacttccagctgtggatgtactgcaccggggcg 134
Score = 87.7 bits (44), Expect = 4e-016
Identities = 83/96 (86%)
Strand = Plus / Minus
Query: 436 gttgtagtcctccacccacatgcccatgctcacgtcctccatcttgaacagccgtaggga 495
||||||||| || |||||||||||||||||||| ||||||||||||||||||| ||| ||
Sbjct: 119 gttgtagtcttcaacccacatgcccatgctcacatcctccatcttgaacagccttagtga 60
Query: 496 gtggtttgtatgccgggatgctacgtctctcgcaat 531
|| |||| || |||||||| | |||||| |||||
Sbjct: 59 ctgatttgcgtgacgggatgcgatgtctctggcaat 24
>gb|AV920212.1|AV920212 AV920212 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags9m07 3', mRNA sequence
Length = 671
Score = 176 bits (89), Expect = 5e-043
Identities = 110/117 (94%)
Strand = Plus / Plus
Query: 314 gacagcttctcccagaggcacagcatctgcctgggggactggtagtgcgccgtgaagtag 373
||||| || |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 555 gacagtttgtcccagaggcacagcatctgcctgggggactggtagtgcgccgtgaagtag 614
Query: 374 ttgtccacgcagccgaactggcagaacctccagctgtggacatactgcacgggggcg 430
||||||||||||||| ||||||||||| |||||||||||| |||||||| ||||||
Sbjct: 615 ttgtccacgcagccgtactggcagaacttccagctgtggatgtactgcaccggggcg 671
>gb|CB878649.1|CB878649 HP09F01T HP Hordeum vulgare subsp. vulgare cDNA clone HP09F01
5-PRIME, mRNA sequence
Length = 196
Score = 127 bits (64), Expect = 4e-028
Identities = 102/112 (91%), Gaps = 2/112 (1%)
Strand = Plus / Minus
Query: 313 tgacagcttctcccagaggcacagcatctgcctgggggact-ggtagtgcgccgtgaagt 371
|||||| || ||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct: 125 tgacagtttgtcccagaggcacagcatctgcctgggggactgggtagtgcgccgtgaagt 66
Query: 372 agttgtccacgcagccgaactggcagaacctccagctgtggacatactgcac 423
|||||||||| |||||| | | ||||||| |||||||||||| ||||||||
Sbjct: 65 agttgtccacccagccgtaat-gcagaacttccagctgtggatgtactgcac 15
>gb|AV920993.1|AV920993 AV920993 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16g24 3', mRNA sequence
Length = 571
Score = 103 bits (52), Expect = 6e-021
Identities = 58/60 (96%)
Strand = Plus / Plus
Query: 314 gacagcttctcccagaggcacagcatctgcctgggggactggtagtgcgccgtgaagtag 373
||||| || |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 512 gacagtttgtcccagaggcacagcatctgcctgggggactggtagtgcgccgtgaagtag 571
>gb|AV833885.1|AV833885 AV833885 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags10a02, mRNA sequence
Length = 681
Score = 58.0 bits (29), Expect = 3e-007
Identities = 41/45 (91%)
Strand = Plus / Plus
Query: 445 ctccacccacatgcccatgctcacgtcctccatcttgaacagccg 489
||||||||||||||||||||| || || ||||||||||| |||||
Sbjct: 473 ctccacccacatgcccatgctgacatcttccatcttgaatagccg 517
>gb|BJ467229.1|BJ467229 BJ467229 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags26d08 3', mRNA sequence
Length = 568
Score = 38.2 bits (19), Expect = 0.29
Identities = 22/23 (95%)
Strand = Plus / Plus
Query: 323 tcccagaggcacagcatctgcct 345
||||||||||||| |||||||||
Sbjct: 428 tcccagaggcacatcatctgcct 450
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 46,304
Number of Sequences: 312970
Number of extensions: 46304
Number of successful extensions: 12686
Number of sequences better than 0.5: 7
Number of HSP's better than 0.5 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 12674
Number of HSP's gapped (non-prelim): 10
length of query: 548
length of database: 175,134,539
effective HSP length: 19
effective length of query: 529
effective length of database: 169,188,109
effective search space: 89500509661
effective search space used: 89500509661
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)