BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.068P22F020924.3.1
(714 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV923358.1|AV923358 AV923358 K. Sato unpublished cDNA li... 385 e-105
gb|BG344237.1|BG344237 HVSMEg0008E18f Hordeum vulgare pre-a... 244 3e-063
gb|BF631054.2|BF631054 HVSMEb0014N14f Hordeum vulgare seedl... 232 1e-059
gb|BG344513.2|BG344513 HVSMEg0009H19f Hordeum vulgare pre-a... 202 1e-050
gb|BG415224.1|BG415224 HVSMEk0005K04f Hordeum vulgare testa... 196 7e-049
gb|AV922764.1|AV922764 AV922764 K. Sato unpublished cDNA li... 196 7e-049
gb|CA009297.1|CA009297 HU13L19r HU Hordeum vulgare subsp. v... 123 8e-027
gb|CA018898.1|CA018898 HV09P17r HV Hordeum vulgare subsp. v... 123 8e-027
gb|AL508626.1|AL508626 AL508626 Hordeum vulgare Barke devel... 48 4e-004
gb|CA022018.1|CA022018 HZ41O17r HZ Hordeum vulgare subsp. v... 46 0.002
gb|BI948219.1|BI948219 HVSMEl0008K13f Hordeum vulgare spike... 40 0.097
gb|BI952021.1|BI952021 HVSMEm0004D23f Hordeum vulgare green... 40 0.097
>gb|AV923358.1|AV923358 AV923358 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd12m24 5', mRNA sequence
Length = 556
Score = 385 bits (194), Expect = e-105
Identities = 377/438 (86%)
Strand = Plus / Plus
Query: 277 tgcttctgactccagtgacagatccaaagacaaacatggagatcaaaagacattgcgccg 336
|||||||||||| ||||||||||||| ||||| | | ||||||||||||||| |||| ||
Sbjct: 73 tgcttctgactctagtgacagatccagagacagaaacggagatcaaaagacaatgcgtcg 132
Query: 337 ccttgctcaaaatcgtgaggctgcaaggaagagccgactaaggaagaaggcatatgttca 396
|||||||||||||| |||||||||||||| || || | ||||| ||||||||||||||
Sbjct: 133 gcttgctcaaaatcgcgaggctgcaaggaaaagtcgtttgaggaaaaaggcatatgttca 192
Query: 397 acagttggagaatagcaggctaaaacttacccaactagagcaagagctgcaaagagctcg 456
||| |||||||||||||||||||| |||||||| |||||||| ||| ||||| |||||||
Sbjct: 193 acaattggagaatagcaggctaaagcttacccagctagagcaggagttgcaacgagctcg 252
Query: 457 tcagcagggcatttttatatctagttcagttgatcagtcacactccatgagtggaaatgg 516
||| || |||||||||||||||||||||| || ||||| || |||||||||||||||||
Sbjct: 253 tcaacaaggcatttttatatctagttcagcagaccagtcccattccatgagtggaaatgg 312
Query: 517 ggcatcggcctttgatatggagtatgcgaggtggttggaagagcacaatcggcaaattag 576
||||| ||| ||||| | |||||| || ||||||||||||| |||||||| ||| |||
Sbjct: 313 ggcattggcttttgacacggagtacgcacggtggttggaagaacacaatcgacaagttaa 372
Query: 577 tgaactaagggctggtgtcagtgctcatgcaagtgatactgatcttcgaagtgttgttga 636
||| || || |||| || | |||||||||| | |||||||| || || |||||||||||
Sbjct: 373 tgagctgagagctgcagttaatgctcatgcaggcgatactgagctgcgcagtgttgttga 432
Query: 637 taagatcatgtcacactatgatgagatttttaggctcaaaggcaatgcagccaaggcaga 696
||||||||||||||||||||||||||||||| || ||||| ||||| ||||| |||||
Sbjct: 433 gaagatcatgtcacactatgatgagatttttaagcaaaaaggaaatgcggccaaagcaga 492
Query: 697 tgtttttcacgtcttatc 714
||| ||||| || |||||
Sbjct: 493 tgtctttcatgtgttatc 510
>gb|BG344237.1|BG344237 HVSMEg0008E18f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0008E18f, mRNA sequence
Length = 766
Score = 244 bits (123), Expect = 3e-063
Identities = 251/291 (86%), Gaps = 2/291 (0%)
Strand = Plus / Plus
Query: 277 tgcttctgactccagtgacagatccaaagacaaacatggagatcaaaagacattgcgccg 336
|||||||||||| ||||||||||||| ||||| | | ||||||||||||||| |||| ||
Sbjct: 382 tgcttctgactctagtgacagatccagagacagaaacggagatcaaaagacaatgcgtcg 441
Query: 337 ccttgctcaaaatcgtgaggctgcaaggaagagccgactaaggaagaaggcatatgttca 396
|||||||||||||| |||||||||||||| || || | | ||| ||||||||||||||
Sbjct: 442 gcttgctcaaaatcgcgaggctgcaaggaaaagtcgtttgaagaaaaaggcatatgttca 501
Query: 397 acagttggagaatagcaggctaaaacttacccaactagagcaagagctgcaaagagctcg 456
||| |||| ||||||||||||||| |||||||| |||||||| ||| ||||| |||||||
Sbjct: 502 acaattggggaatagcaggctaaagcttacccagctagagcaggaggtgcaacgagctcg 561
Query: 457 tcagcagggcatttttatatctagttcagttgatcagtcacactccatgagtggaaatgg 516
||| || ||||||||||||||||||||| || ||||| || |||||||||||||||||
Sbjct: 562 tcaaca-agcatttttatatctagttcagcagaccagtcccattccatgagtggaaatgg 620
Query: 517 ggcatcggcctttgatatggagtatgcgaggtggttggaagagcacaatcg 567
||||| ||| ||||| | |||||| || | |||| ||||||| ||||||||
Sbjct: 621 ggcattggcttttgacacggagtacgc-acgtgggtggaagaacacaatcg 670
Score = 46.1 bits (23), Expect = 0.002
Identities = 43/49 (87%), Gaps = 3/49 (6%)
Strand = Plus / Plus
Query: 189 caatggcagatgccagccctagaacagaaacatcaacagatgatactga 237
|||||||||| ||||| |||||||||||||| ||| |||||||||||
Sbjct: 294 caatggcagaggccagtcctagaacagaaacgtca---gatgatactga 339
>gb|BF631054.2|BF631054 HVSMEb0014N14f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0014N14f, mRNA sequence
Length = 768
Score = 232 bits (117), Expect = 1e-059
Identities = 186/209 (88%)
Strand = Plus / Plus
Query: 277 tgcttctgactccagtgacagatccaaagacaaacatggagatcaaaagacattgcgccg 336
|||||||||||| ||||||||||||| ||||| | | ||||||||||||||| |||| ||
Sbjct: 419 tgcttctgactctagtgacagatccagagacagaaacggagatcaaaagacaatgcgtcg 478
Query: 337 ccttgctcaaaatcgtgaggctgcaaggaagagccgactaaggaagaaggcatatgttca 396
|||||||||||||| |||||||||||||| || || | ||||| ||||||||||||||
Sbjct: 479 gcttgctcaaaatcgcgaggctgcaaggaaaagtcgtttgaggaaaaaggcatatgttca 538
Query: 397 acagttggagaatagcaggctaaaacttacccaactagagcaagagctgcaaagagctcg 456
||| |||||||||||||||||||| |||||||| |||||||| ||| ||||| |||||||
Sbjct: 539 acaattggagaatagcaggctaaagcttacccagctagagcaggagttgcaacgagctcg 598
Query: 457 tcagcagggcatttttatatctagttcag 485
||| || ||||||||||||||||||||||
Sbjct: 599 tcaacaaggcatttttatatctagttcag 627
Score = 46.1 bits (23), Expect = 0.002
Identities = 43/49 (87%), Gaps = 3/49 (6%)
Strand = Plus / Plus
Query: 189 caatggcagatgccagccctagaacagaaacatcaacagatgatactga 237
|||||||||| ||||| |||||||||||||| ||| |||||||||||
Sbjct: 331 caatggcagaggccagtcctagaacagaaacgtca---gatgatactga 376
>gb|BG344513.2|BG344513 HVSMEg0009H19f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0009H19f, mRNA sequence
Length = 798
Score = 202 bits (102), Expect = 1e-050
Identities = 216/254 (85%)
Strand = Plus / Plus
Query: 461 cagggcatttttatatctagttcagttgatcagtcacactccatgagtggaaatggggca 520
||||||||||||||||||||||||| || ||||| || |||||||||||||||||||||
Sbjct: 264 cagggcatttttatatctagttcagcagaccagtcccattccatgagtggaaatggggca 323
Query: 521 tcggcctttgatatggagtatgcgaggtggttggaagagcacaatcggcaaattagtgaa 580
| ||| ||||| | |||||| || ||||||||||||| |||||||| ||| ||| |||
Sbjct: 324 ttggcttttgacacggagtacgcacggtggttggaagaacacaatcgacaagttaatgag 383
Query: 581 ctaagggctggtgtcagtgctcatgcaagtgatactgatcttcgaagtgttgttgataag 640
|| || |||| || | |||||||||| | |||||||| || || ||||||||||| |||
Sbjct: 384 ctgagagctgcagttaatgctcatgcaggcgatactgagctgcgcagtgttgttgagaag 443
Query: 641 atcatgtcacactatgatgagatttttaggctcaaaggcaatgcagccaaggcagatgtt 700
|||||||||||||||||||||||||||| || ||||| ||||| ||||| ||||||||
Sbjct: 444 atcatgtcacactatgatgagatttttaagcaaaaaggaaatgcggccaaagcagatgtc 503
Query: 701 tttcacgtcttatc 714
||||| || |||||
Sbjct: 504 tttcatgtgttatc 517
Score = 188 bits (95), Expect = 2e-046
Identities = 158/179 (88%)
Strand = Plus / Plus
Query: 281 tctgactccagtgacagatccaaagacaaacatggagatcaaaagacattgcgccgcctt 340
|||||||| ||||||||||||| ||||| | | ||||||||||||||| |||| || |||
Sbjct: 1 tctgactctagtgacagatccagagacagaaacggagatcaaaagacaatgcgtcggctt 60
Query: 341 gctcaaaatcgtgaggctgcaaggaagagccgactaaggaagaaggcatatgttcaacag 400
||||||||||| |||||||||||||| || || | ||||| |||||||||||||||||
Sbjct: 61 gctcaaaatcgcgaggctgcaaggaaaagtcgtttgaggaaaaaggcatatgttcaacaa 120
Query: 401 ttggagaatagcaggctaaaacttacccaactagagcaagagctgcaaagagctcgtca 459
|||||||||||||||||||| |||||||| |||||||| ||| ||||| ||||||||||
Sbjct: 121 ttggagaatagcaggctaaagcttacccagctagagcaggagttgcaacgagctcgtca 179
>gb|BG415224.1|BG415224 HVSMEk0005K04f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0005K04f, mRNA sequence
Length = 898
Score = 196 bits (99), Expect = 7e-049
Identities = 162/183 (88%)
Strand = Plus / Plus
Query: 277 tgcttctgactccagtgacagatccaaagacaaacatggagatcaaaagacattgcgccg 336
|||||||||||| ||||||||||||| ||||| | | ||||||||||||||| |||| ||
Sbjct: 305 tgcttctgactctagtgacagatccagagacagaaacggagatcaaaagacaatgcgtcg 364
Query: 337 ccttgctcaaaatcgtgaggctgcaaggaagagccgactaaggaagaaggcatatgttca 396
|||||||||||||| |||||||||||||| || || | ||||| ||||||||||||||
Sbjct: 365 gcttgctcaaaatcgcgaggctgcaaggaaaagtcgtttgaggaaaaaggcatatgttca 424
Query: 397 acagttggagaatagcaggctaaaacttacccaactagagcaagagctgcaaagagctcg 456
||| |||||||||||||||||||| |||||||| |||||||| ||| ||||| |||||||
Sbjct: 425 acaattggagaatagcaggctaaagcttacccagctagagcaggagttgcaacgagctcg 484
Query: 457 tca 459
|||
Sbjct: 485 tca 487
Score = 182 bits (92), Expect = 1e-044
Identities = 179/208 (86%)
Strand = Plus / Plus
Query: 461 cagggcatttttatatctagttcagttgatcagtcacactccatgagtggaaatggggca 520
||||||||||||||||||||||||| || ||||| || |||||||||||||||||||||
Sbjct: 572 cagggcatttttatatctagttcagcagaccagtcccattccatgagtggaaatggggca 631
Query: 521 tcggcctttgatatggagtatgcgaggtggttggaagagcacaatcggcaaattagtgaa 580
| ||| ||||| | |||||| || ||||||||||||| |||||||| ||| ||| |||
Sbjct: 632 ttggcttttgacacggagtacgcacggtggttggaagaacacaatcgacaagttaatgag 691
Query: 581 ctaagggctggtgtcagtgctcatgcaagtgatactgatcttcgaagtgttgttgataag 640
|| || |||| || | |||||||||| | |||||||| || || ||||||||||| |||
Sbjct: 692 ctgagagctgcagttaatgctcatgcaggcgatactgagctgcgcagtgttgttgagaag 751
Query: 641 atcatgtcacactatgatgagattttta 668
||||||||||||||||||||||||||||
Sbjct: 752 atcatgtcacactatgatgagattttta 779
Score = 46.1 bits (23), Expect = 0.002
Identities = 43/49 (87%), Gaps = 3/49 (6%)
Strand = Plus / Plus
Query: 189 caatggcagatgccagccctagaacagaaacatcaacagatgatactga 237
|||||||||| ||||| |||||||||||||| ||| |||||||||||
Sbjct: 217 caatggcagaggccagtcctagaacagaaacgtca---gatgatactga 262
>gb|AV922764.1|AV922764 AV922764 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd2e15 5', mRNA sequence
Length = 652
Score = 196 bits (99), Expect = 7e-049
Identities = 162/183 (88%)
Strand = Plus / Plus
Query: 277 tgcttctgactccagtgacagatccaaagacaaacatggagatcaaaagacattgcgccg 336
|||||||||||| ||||||||||||| ||||| | | ||||||||||||||| |||| ||
Sbjct: 381 tgcttctgactctagtgacagatccagagacagaaacggagatcaaaagacaatgcgtcg 440
Query: 337 ccttgctcaaaatcgtgaggctgcaaggaagagccgactaaggaagaaggcatatgttca 396
|||||||||||||| |||||||||||||| || || | ||||| ||||||||||||||
Sbjct: 441 gcttgctcaaaatcgcgaggctgcaaggaaaagtcgtttgaggaaaaaggcatatgttca 500
Query: 397 acagttggagaatagcaggctaaaacttacccaactagagcaagagctgcaaagagctcg 456
||| |||||||||||||||||||| |||||||| |||||||| ||| ||||| |||||||
Sbjct: 501 acaattggagaatagcaggctaaagcttacccagctagagcaggagttgcaacgagctcg 560
Query: 457 tca 459
|||
Sbjct: 561 tca 563
Score = 46.1 bits (23), Expect = 0.002
Identities = 43/49 (87%), Gaps = 3/49 (6%)
Strand = Plus / Plus
Query: 189 caatggcagatgccagccctagaacagaaacatcaacagatgatactga 237
|||||||||| ||||| |||||||||||||| ||| |||||||||||
Sbjct: 293 caatggcagaggccagtcctagaacagaaacgtca---gatgatactga 338
>gb|CA009297.1|CA009297 HU13L19r HU Hordeum vulgare subsp. vulgare cDNA clone HU13L19
5-PRIME, mRNA sequence
Length = 625
Score = 123 bits (62), Expect = 8e-027
Identities = 125/146 (85%)
Strand = Plus / Plus
Query: 320 caaaagacattgcgccgccttgctcaaaatcgtgaggctgcaaggaagagccgactaagg 379
|||||||||||||| || ||||||||||||||||||||||||||||| || |||||||||
Sbjct: 36 caaaagacattgcgtcgtcttgctcaaaatcgtgaggctgcaaggaaaagtcgactaagg 95
Query: 380 aagaaggcatatgttcaacagttggagaatagcaggctaaaacttacccaactagagcaa 439
|||||| ||||||| |||||| | || | |||| ||| || ||| | |||||||||||
Sbjct: 96 aagaagtcatatgtgcaacagctagaaagcagcaagctgaagcttgcacaactagagcag 155
Query: 440 gagctgcaaagagctcgtcagcaggg 465
||||| || | |||||| ||||||||
Sbjct: 156 gagctccagaaagctcgccagcaggg 181
Score = 38.2 bits (19), Expect = 0.38
Identities = 31/35 (88%)
Strand = Plus / Plus
Query: 644 atgtcacactatgatgagatttttaggctcaaagg 678
|||||||| ||||||||||| |||| | |||||||
Sbjct: 360 atgtcacattatgatgagatatttaaggtcaaagg 394
>gb|CA018898.1|CA018898 HV09P17r HV Hordeum vulgare subsp. vulgare cDNA clone HV09P17
5-PRIME, mRNA sequence
Length = 414
Score = 123 bits (62), Expect = 8e-027
Identities = 125/146 (85%)
Strand = Plus / Plus
Query: 320 caaaagacattgcgccgccttgctcaaaatcgtgaggctgcaaggaagagccgactaagg 379
|||||||||||||| || ||||||||||||||||||||||||||||| || |||||||||
Sbjct: 76 caaaagacattgcgtcgtcttgctcaaaatcgtgaggctgcaaggaaaagtcgactaagg 135
Query: 380 aagaaggcatatgttcaacagttggagaatagcaggctaaaacttacccaactagagcaa 439
|||||| ||||||| |||||| | || | |||| ||| || ||| | |||||||||||
Sbjct: 136 aagaagtcatatgtgcaacagctagaaagcagcaagctgaagcttgcacaactagagcag 195
Query: 440 gagctgcaaagagctcgtcagcaggg 465
||||| || | |||||| ||||||||
Sbjct: 196 gagctccagaaagctcgccagcaggg 221
>gb|AL508626.1|AL508626 AL508626 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY09F05V 5',
mRNA sequence
Length = 622
Score = 48.1 bits (24), Expect = 4e-004
Identities = 37/40 (92%), Gaps = 1/40 (2%)
Strand = Plus / Plus
Query: 320 caaaagacattgcgccgcctt-gctcaaaatcgtgaggct 358
|||||||||||||| || ||| ||||||||||||||||||
Sbjct: 558 caaaagacattgcgtcgtctttgctcaaaatcgtgaggct 597
>gb|CA022018.1|CA022018 HZ41O17r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ41O17
5-PRIME, mRNA sequence
Length = 149
Score = 46.1 bits (23), Expect = 0.002
Identities = 43/49 (87%), Gaps = 3/49 (6%)
Strand = Plus / Plus
Query: 189 caatggcagatgccagccctagaacagaaacatcaacagatgatactga 237
|||||||||| ||||| |||||||||||||| ||| |||||||||||
Sbjct: 91 caatggcagaggccagtcctagaacagaaacgtca---gatgatactga 136
>gb|BI948219.1|BI948219 HVSMEl0008K13f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0008K13f, mRNA sequence
Length = 851
Score = 40.1 bits (20), Expect = 0.097
Identities = 32/36 (88%)
Strand = Plus / Plus
Query: 350 cgtgaggctgcaaggaagagccgactaaggaagaag 385
|||||||||||||||| || | |||||||||||||
Sbjct: 588 cgtgaggctgcaaggagaagtccactaaggaagaag 623
>gb|BI952021.1|BI952021 HVSMEm0004D23f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0004D23f, mRNA sequence
Length = 891
Score = 40.1 bits (20), Expect = 0.097
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 66 cttcccctcccgcctctcgc 85
||||||||||||||||||||
Sbjct: 872 cttcccctcccgcctctcgc 853
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 67,711
Number of Sequences: 312970
Number of extensions: 67711
Number of successful extensions: 19657
Number of sequences better than 0.5: 12
Number of HSP's better than 0.5 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 19622
Number of HSP's gapped (non-prelim): 30
length of query: 714
length of database: 175,134,539
effective HSP length: 19
effective length of query: 695
effective length of database: 169,188,109
effective search space: 117585735755
effective search space used: 117585735755
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)