BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.064G21F020918.3.1
(705 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV836405.1|AV836405 AV836405 K. Sato unpublished cDNA li... 159 2e-037
gb|BQ466537.1|BQ466537 HT02N03r HT Hordeum vulgare subsp. v... 56 2e-006
>gb|AV836405.1|AV836405 AV836405 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd14a23, mRNA
sequence
Length = 641
Score = 159 bits (80), Expect = 2e-037
Identities = 216/266 (81%)
Strand = Plus / Plus
Query: 440 aatgggaaagggtgatgtggctactaggtccaaatctcaaaaatcgtcagcaatacagaa 499
|||||||||||| ||||||| || ||||||||||| || |||||||| || |||||||
Sbjct: 243 aatgggaaagggatatgtggccacgaggtccaaatcccagaaatcgtcgacagtacagaa 302
Query: 500 tgagccgagtacacctgctaannnnnnnacagcatatcctgattggtctcagttccaggc 559
||| | |||||||||| |||| |||||||||||||| ||||| ||||| |||||
Sbjct: 303 tgaacagagtacacctactaatcctcctacagcatatcctgactggtcccagtttcaggc 362
Query: 560 atactataatgctgctgggacagctccagtaaccccaccggcnnnnnnncattcatctgt 619
||||||||||| |||| || |||| | | ||||||||||| |||||| | ||
Sbjct: 363 atactataatgtccctggcacggctcaaatgaccccaccggcttattttcattcaacggt 422
Query: 620 agctccaacccatcaggggcacccgtatatgtggggtccacagatgatgcctccttatgg 679
||||||| | ||||||||| || |||||||||||||||||||||||||||||||| ||
Sbjct: 423 ggctccaagtcctcaggggcatccatatatgtggggtccacagatgatgcctccttacgg 482
Query: 680 gacaccaccaccatatgcaacaatgt 705
||| || |||||||| ||||| ||||
Sbjct: 483 gacgccgccaccatacgcaacgatgt 508
>gb|BQ466537.1|BQ466537 HT02N03r HT Hordeum vulgare subsp. vulgare cDNA clone HT02N03
5-PRIME, mRNA sequence
Length = 544
Score = 56.0 bits (28), Expect = 2e-006
Identities = 43/48 (89%)
Strand = Plus / Plus
Query: 440 aatgggaaagggtgatgtggctactaggtccaaatctcaaaaatcgtc 487
|||||||||||| |||||||| || ||||||||||| || ||||||||
Sbjct: 389 aatgggaaagggagatgtggccacgaggtccaaatcccagaaatcgtc 436
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 79,070
Number of Sequences: 312970
Number of extensions: 79070
Number of successful extensions: 21298
Number of sequences better than 0.5: 2
Number of HSP's better than 0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 21293
Number of HSP's gapped (non-prelim): 4
length of query: 705
length of database: 175,134,539
effective HSP length: 19
effective length of query: 686
effective length of database: 169,188,109
effective search space: 116063042774
effective search space used: 116063042774
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)