BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBN21e06.xg.2.1
(704 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ459891.1|BJ459891 BJ459891 K. Sato unpublished cDNA li... 204 3e-051
gb|CB858305.1|CB858305 HI06I11w HI Hordeum vulgare subsp. v... 178 2e-043
gb|BQ470070.1|BQ470070 HX01K23T HX Hordeum vulgare subsp. v... 151 4e-035
gb|BE438011.1|BE438011 SFR009.H06F990511 ITEC SFR Barley Le... 133 9e-030
gb|BJ447934.1|BJ447934 BJ447934 K. Sato unpublished cDNA li... 133 9e-030
gb|BJ455677.1|BJ455677 BJ455677 K. Sato unpublished cDNA li... 133 9e-030
gb|AL499972.1|AL499972 AL499972 Hordeum vulgare Barke etiol... 113 8e-024
gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA li... 94 7e-018
gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedl... 54 6e-006
gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Li... 46 0.002
gb|CA006891.1|CA006891 HU06A02r HU Hordeum vulgare subsp. v... 44 0.006
gb|AL509042.1|AL509042 AL509042 Hordeum vulgare Barke devel... 42 0.024
gb|BE060229.3|BE060229 HVSMEg0011K10f Hordeum vulgare pre-a... 42 0.024
>gb|BJ459891.1|BJ459891 BJ459891 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38k12 3', mRNA sequence
Length = 659
Score = 204 bits (103), Expect = 3e-051
Identities = 367/455 (80%)
Strand = Plus / Minus
Query: 32 tggctcccctgaagctgctacacttgttcgctggatttgccaaagctaccggtatcactt 91
||||| |||||||||||| ||||||| ||||||||| |||||||||||||||| || |
Sbjct: 659 tggcttccctgaagctgcaacacttgctcgctggatatgccaaagctaccggttccatct 600
Query: 92 gggggcggatttggtcagccactcggatcaagctggggaatcacttttgacaatgttctg 151
||||| || || | || || | ||| ||||||||||||| | |||| |||| ||||
Sbjct: 599 gggggtcgagttatttagacaggcagatgaagctggggaatctttattgagaatgctctg 540
Query: 152 ggaccatcaagacgctgtcttgtgctgctctttcaaggaaaagcctgtgtttactttcgg 211
||| ||| |||| ||| | |||||||| |||||||||||||||||||| ||||| || |
Sbjct: 539 ggatcatgaagatgctattttgtgctgttctttcaaggaaaagcctgtatttacgtttgc 480
Query: 212 caaccagatgggagttgacatgttagaaacgactttagtcgctctgcaagatctcaccct 271
||| ||||||| || ||||||| ||||| |||| || || || ||||| ||| | |
Sbjct: 479 aaacgagatggggattaacatgttggaaacatctttcgttgcccttcaagacctctcgtt 420
Query: 272 tgacaagatctctgacgagcctggtagaaaggcactacatgcggaggttccgaaactgat 331
|||||||| | ||| || ||||||||||||||||| | | ||| | || || ||||
Sbjct: 419 ggacaagatatttgatgaagctggtagaaaggcactatactctgagatcccaaagttgat 360
Query: 332 ggaacagggctatgcgtacctgccggctggggtgtgcctgtctgggatgggccgccatgt 391
||| ||||||| || |||||||| | ||| |||||| ||||||||||||||||||||||
Sbjct: 359 ggagcagggctttgtttacctgccaggtggtgtgtgcttgtctgggatgggccgccatgt 300
Query: 392 ctcctacgagcaagctgttgcttggaaagttctcggtgaggacggcaacgtgcactgcct 451
||||| |||| | ||| | || |||||||| ||||||||||| ||| |||||||||||
Sbjct: 299 ctccttcgagaatgctatagcatggaaagtagtcggtgaggacaacaatgtgcactgcct 240
Query: 452 cgccttctgtttcgtcaactggtccttcgtctgat 486
|||||||| |||||||||||||| ||||| ||||
Sbjct: 239 tgccttctgcttcgtcaactggtctttcgtgtgat 205
>gb|CB858305.1|CB858305 HI06I11w HI Hordeum vulgare subsp. vulgare cDNA clone HI06I11
3-PRIME, mRNA sequence
Length = 638
Score = 178 bits (90), Expect = 2e-043
Identities = 297/366 (81%)
Strand = Plus / Minus
Query: 121 aagctggggaatcacttttgacaatgttctgggaccatcaagacgctgtcttgtgctgct 180
||||||||||||| | |||| |||| ||||||| ||| |||| ||| | |||||||| |
Sbjct: 562 aagctggggaatctttattgagaatgctctgggatcatgaagatgctattttgtgctgtt 503
Query: 181 ctttcaaggaaaagcctgtgtttactttcggcaaccagatgggagttgacatgttagaaa 240
||||||||||||||||||| ||||| || | ||| ||||||| || ||||||| ||||
Sbjct: 502 ctttcaaggaaaagcctgtatttacgtttgcaaacgagatggggattaacatgttggaaa 443
Query: 241 cgactttagtcgctctgcaagatctcacccttgacaagatctctgacgagcctggtagaa 300
| |||| || || || ||||| ||| | | |||||||| | ||| || |||||||||
Sbjct: 442 catctttcgttgcccttcaagacctctcgttggacaagatatttgatgaagctggtagaa 383
Query: 301 aggcactacatgcggaggttccgaaactgatggaacagggctatgcgtacctgccggctg 360
|||||||| | | ||| | || || ||||||| ||||||| || |||||||| | ||
Sbjct: 382 aggcactatactctgagatcccaaagttgatggagcagggctttgtttacctgccaggtg 323
Query: 361 gggtgtgcctgtctgggatgggccgccatgtctcctacgagcaagctgttgcttggaaag 420
| |||||| ||||||||||||||||||||||||||| |||| | ||| | || |||||||
Sbjct: 322 gtgtgtgcttgtctgggatgggccgccatgtctccttcgagaatgctatagcatggaaag 263
Query: 421 ttctcggtgaggacggcaacgtgcactgcctcgccttctgtttcgtcaactggtccttcg 480
| ||||||||||| ||| ||||||||||| |||||||| |||||||||||||| ||||
Sbjct: 262 tagtcggtgaggacaacaatgtgcactgccttgccttctgcttcgtcaactggtctttcg 203
Query: 481 tctgat 486
| ||||
Sbjct: 202 tgtgat 197
>gb|BQ470070.1|BQ470070 HX01K23T HX Hordeum vulgare subsp. vulgare cDNA clone HX01K23
5-PRIME, mRNA sequence
Length = 633
Score = 151 bits (76), Expect = 4e-035
Identities = 289/360 (80%)
Strand = Plus / Plus
Query: 121 aagctggggaatcacttttgacaatgttctgggaccatcaagacgctgtcttgtgctgct 180
||||||||||||| | |||| |||| ||||||| ||| |||| ||| | |||||||| |
Sbjct: 59 aagctggggaatctttattgagaatgctctgggatcatgaagatgctattttgtgctgtt 118
Query: 181 ctttcaaggaaaagcctgtgtttactttcggcaaccagatgggagttgacatgttagaaa 240
||||||||||||||||||| ||||| || | ||| ||||||| || ||||||| ||||
Sbjct: 119 ctttcaaggaaaagcctgtatttacgtttgcaaacgagatggggattaacatgttggaaa 178
Query: 241 cgactttagtcgctctgcaagatctcacccttgacaagatctctgacgagcctggtagaa 300
| |||| || || ||||| ||| | | |||||||| | ||| || |||||||||
Sbjct: 179 catctttcgttgccactcaagacctctcgttggacaagatatttgatgaagctggtagaa 238
Query: 301 aggcactacatgcggaggttccgaaactgatggaacagggctatgcgtacctgccggctg 360
|||||||| | | ||| | || || ||||||| ||||||| || |||||||| | ||
Sbjct: 239 aggcactatactctgagatcccaaagttgatggagcagggctttgtttacctgccaggtg 298
Query: 361 gggtgtgcctgtctgggatgggccgccatgtctcctacgagcaagctgttgcttggaaag 420
| |||||| ||||||||||||||||||||||||||| |||| | ||| | || |||||||
Sbjct: 299 gtgtgtgcttgtctgggatgggccgccatgtctccttcgagaatgctatagcatggaaag 358
Query: 421 ttctcggtgaggacggcaacgtgcactgcctcgccttctgtttcgtcaactggtccttcg 480
| ||||||||||| ||| ||||||||||| |||||||| || ||||||||||| ||||
Sbjct: 359 tagtcggtgaggacaacaatgtgcactgccttgccttctgcttagtcaactggtctttcg 418
>gb|BE438011.1|BE438011 SFR009.H06F990511 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 523
Score = 133 bits (67), Expect = 9e-030
Identities = 136/159 (85%)
Strand = Plus / Plus
Query: 328 tgatggaacagggctatgcgtacctgccggctggggtgtgcctgtctgggatgggccgcc 387
||||||| ||||||| || |||||||| | ||| |||||| ||||||||||||||||||
Sbjct: 85 tgatggagcagggctttgtttacctgccaggtggtgtgtgcttgtctgggatgggccgcc 144
Query: 388 atgtctcctacgagcaagctgttgcttggaaagttctcggtgaggacggcaacgtgcact 447
||||||||| |||| | ||| | || |||||||| ||||||||||| ||| |||||||
Sbjct: 145 atgtctccttcgagaatgctatagcatggaaagtagtcggtgaggacaacaatgtgcact 204
Query: 448 gcctcgccttctgtttcgtcaactggtccttcgtctgat 486
|||| |||||||| |||||||||||||| ||||| ||||
Sbjct: 205 gccttgccttctgcttcgtcaactggtctttcgtgtgat 243
>gb|BJ447934.1|BJ447934 BJ447934 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17m20 5', mRNA sequence
Length = 447
Score = 133 bits (67), Expect = 9e-030
Identities = 136/159 (85%)
Strand = Plus / Plus
Query: 328 tgatggaacagggctatgcgtacctgccggctggggtgtgcctgtctgggatgggccgcc 387
||||||| ||||||| || |||||||| | ||| |||||| ||||||||||||||||||
Sbjct: 62 tgatggagcagggctttgtttacctgccaggtggtgtgtgcttgtctgggatgggccgcc 121
Query: 388 atgtctcctacgagcaagctgttgcttggaaagttctcggtgaggacggcaacgtgcact 447
||||||||| |||| | ||| | || |||||||| ||||||||||| ||| |||||||
Sbjct: 122 atgtctccttcgagaatgctatagcatggaaagtagtcggtgaggacaacaatgtgcact 181
Query: 448 gcctcgccttctgtttcgtcaactggtccttcgtctgat 486
|||| |||||||| |||||||||||||| ||||| ||||
Sbjct: 182 gccttgccttctgcttcgtcaactggtctttcgtgtgat 220
>gb|BJ455677.1|BJ455677 BJ455677 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17m20 3', mRNA sequence
Length = 420
Score = 133 bits (67), Expect = 9e-030
Identities = 136/159 (85%)
Strand = Plus / Minus
Query: 328 tgatggaacagggctatgcgtacctgccggctggggtgtgcctgtctgggatgggccgcc 387
||||||| ||||||| || |||||||| | ||| |||||| ||||||||||||||||||
Sbjct: 360 tgatggagcagggctttgtttacctgccaggtggtgtgtgcttgtctgggatgggccgcc 301
Query: 388 atgtctcctacgagcaagctgttgcttggaaagttctcggtgaggacggcaacgtgcact 447
||||||||| |||| | ||| | || |||||||| ||||||||||| ||| |||||||
Sbjct: 300 atgtctccttcgagaatgctatagcatggaaagtagtcggtgaggacaacaatgtgcact 241
Query: 448 gcctcgccttctgtttcgtcaactggtccttcgtctgat 486
|||| |||||||| |||||||||||||| ||||| ||||
Sbjct: 240 gccttgccttctgcttcgtcaactggtctttcgtgtgat 202
>gb|AL499972.1|AL499972 AL499972 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK04F09r 3', mRNA sequence
Length = 573
Score = 113 bits (57), Expect = 8e-024
Identities = 135/160 (84%), Gaps = 1/160 (0%)
Strand = Plus / Minus
Query: 328 tgatggaacagggctatgcgtacctgccggctggggtgtgcctgtct-gggatgggccgc 386
||||||| ||||||| || |||||||| | ||| |||||| ||||| ||||||||||||
Sbjct: 412 tgatggagcagggctttgtttacctgccaggtggtgtgtgcttgtcttgggatgggccgc 353
Query: 387 catgtctcctacgagcaagctgttgcttggaaagttctcggtgaggacggcaacgtgcac 446
|||||||||| |||| | ||| | || |||||||| ||||||||||| ||| |||||
Sbjct: 352 catgtctccttcgagaatgctatagcatggaaagtagtcggtgaggacaacaatgtgcan 293
Query: 447 tgcctcgccttctgtttcgtcaactggtccttcgtctgat 486
||||| |||||||| |||||||||||||| ||||| ||||
Sbjct: 292 tgccttgccttctgcttcgtcaactggtctttcgtgtgat 253
>gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38k12 5', mRNA sequence
Length = 633
Score = 93.7 bits (47), Expect = 7e-018
Identities = 62/67 (92%)
Strand = Plus / Plus
Query: 18 cagaagctaatttctggctcccctgaagctgctacacttgttcgctggatttgccaaagc 77
|||||| |||||||||||| |||||||||||| ||||||| ||||||||| |||||||||
Sbjct: 472 cagaagataatttctggcttccctgaagctgcaacacttgctcgctggatatgccaaagc 531
Query: 78 taccggt 84
|||||||
Sbjct: 532 taccggt 538
>gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0001C22f, mRNA sequence
Length = 834
Score = 54.0 bits (27), Expect = 6e-006
Identities = 36/39 (92%)
Strand = Plus / Minus
Query: 443 gcactgcctcgccttctgtttcgtcaactggtccttcgt 481
|||||||||||||||| ||||||||||||||||||||
Sbjct: 314 gcactgcctcgccttcatgttcgtcaactggtccttcgt 276
>gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM011cD.08, mRNA sequence
Length = 1501
Score = 46.1 bits (23), Expect = 0.002
Identities = 35/39 (89%)
Strand = Plus / Plus
Query: 443 gcactgcctcgccttctgtttcgtcaactggtccttcgt 481
||||||||||||||| | |||||||||||||||||||
Sbjct: 707 gcactgcctcgcctttcatgtcgtcaactggtccttcgt 745
>gb|CA006891.1|CA006891 HU06A02r HU Hordeum vulgare subsp. vulgare cDNA clone HU06A02
5-PRIME, mRNA sequence
Length = 322
Score = 44.1 bits (22), Expect = 0.006
Identities = 31/34 (91%)
Strand = Plus / Minus
Query: 453 gccttctgtttcgtcaactggtccttcgtctgat 486
|||||||| |||||||||||||| ||||| ||||
Sbjct: 318 gccttctgcttcgtcaactggtctttcgtgtgat 285
>gb|AL509042.1|AL509042 AL509042 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY10I20V 5',
mRNA sequence
Length = 700
Score = 42.1 bits (21), Expect = 0.024
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 37 cccctgaagctgctacacttg 57
|||||||||||||||||||||
Sbjct: 433 cccctgaagctgctacacttg 413
>gb|BE060229.3|BE060229 HVSMEg0011K10f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011K10f, mRNA sequence
Length = 594
Score = 42.1 bits (21), Expect = 0.024
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 37 cccctgaagctgctacacttg 57
|||||||||||||||||||||
Sbjct: 263 cccctgaagctgctacacttg 243
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 60,552
Number of Sequences: 312970
Number of extensions: 60552
Number of successful extensions: 17576
Number of sequences better than 0.5: 13
Number of HSP's better than 0.5 without gapping: 13
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 17547
Number of HSP's gapped (non-prelim): 27
length of query: 704
length of database: 175,134,539
effective HSP length: 19
effective length of query: 685
effective length of database: 169,188,109
effective search space: 115893854665
effective search space used: 115893854665
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)