BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBL16c05.xg.2.1
(687 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF261857.2|BF261857 HV_CEa0002G15f Hordeum vulgare seedl... 96 2e-018
gb|BF617439.1|BF617439 HVSMEc0017F20f Hordeum vulgare seedl... 90 1e-016
gb|BF618630.2|BF618630 HVSMEc0006O07f Hordeum vulgare seedl... 86 2e-015
gb|BU998112.1|BU998112 HI10A14r HI Hordeum vulgare subsp. v... 82 3e-014
gb|BQ463523.1|BQ463523 HI05J08r HI Hordeum vulgare subsp. v... 52 2e-005
gb|BI958189.1|BI958189 HVSMEn0013M24f Hordeum vulgare rachi... 50 1e-004
gb|BG343139.1|BG343139 HVSMEg0002L02f Hordeum vulgare pre-a... 38 0.37
gb|CA022106.1|CA022106 HZ42C22r HZ Hordeum vulgare subsp. v... 38 0.37
>gb|BF261857.2|BF261857 HV_CEa0002G15f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0002G15f, mRNA sequence
Length = 853
Score = 95.6 bits (48), Expect = 2e-018
Identities = 156/192 (81%)
Strand = Plus / Plus
Query: 329 ctctggtcccctgaggaggatgagaaactcatgaatcacataacaaagcatggccatggc 388
|||||||| || |||||||| ||||| || ||||| ||||| | ||| | ||| | |||
Sbjct: 239 ctctggtcgccggaggaggacgagaagctgatgaaccacattgccaagtacggcaacggc 298
Query: 389 tgctggagctctgttcccaaactcgctgggcttcaaaggtgcggaaagagttgtaggctg 448
||||||||||| || ||||| | || || ||| | |||||||| ||||| || ||||||
Sbjct: 299 tgctggagctccgtccccaagattgcaggccttgagaggtgcgggaagagctgcaggctg 358
Query: 449 aggtggataaactatctgaggccagacctcaagagaggggcattcgcccaagacgaagaa 508
|||||||||||||| |||||||| ||||||||||| || || ||| | || || || ||
Sbjct: 359 aggtggataaactacctgaggccggacctcaagaggggcgccttctcgcaggaggaggag 418
Query: 509 gacctcatcatc 520
||||||||||||
Sbjct: 419 gacctcatcatc 430
>gb|BF617439.1|BF617439 HVSMEc0017F20f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0017F20f, mRNA sequence
Length = 786
Score = 89.7 bits (45), Expect = 1e-016
Identities = 157/193 (81%), Gaps = 1/193 (0%)
Strand = Plus / Plus
Query: 329 ctctggtcccctgaggaggatgagaaa-ctcatgaatcacataacaaagcatggccatgg 387
|||||||| || |||||||| |||||| || ||||| ||||| | ||| | ||| | ||
Sbjct: 158 ctctggtcgccggaggaggacgagaaagctgatgaaccacattgccaagtacggcaacgg 217
Query: 388 ctgctggagctctgttcccaaactcgctgggcttcaaaggtgcggaaagagttgtaggct 447
|||||||||||| || ||||| | || || ||| | |||||||| ||||| || |||||
Sbjct: 218 ctgctggagctccgtccccaagattgcaggccttgagaggtgcgggaagagctgcaggct 277
Query: 448 gaggtggataaactatctgaggccagacctcaagagaggggcattcgcccaagacgaaga 507
||||||||||||||| |||||||| ||||||||||| || || ||| | || || || ||
Sbjct: 278 gaggtggataaactacctgaggccggacctcaagaggggcgccttctcgcaggaggagga 337
Query: 508 agacctcatcatc 520
||||||||||||
Sbjct: 338 ggacctcatcatc 350
>gb|BF618630.2|BF618630 HVSMEc0006O07f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0006O07f, mRNA sequence
Length = 556
Score = 85.7 bits (43), Expect = 2e-015
Identities = 154/191 (80%)
Strand = Plus / Plus
Query: 329 ctctggtcccctgaggaggatgagaaactcatgaatcacataacaaagcatggccatggc 388
|||||||| || |||||||| |||| || ||||| ||||| | ||| | ||| | |||
Sbjct: 162 ctctggtcgccggaggaggactagaagctgatgaaccacattgccaagtacggcaacggc 221
Query: 389 tgctggagctctgttcccaaactcgctgggcttcaaaggtgcggaaagagttgtaggctg 448
||||||||||| || ||||| | || || ||| | |||||||| ||||| || ||||||
Sbjct: 222 tgctggagctccgtccccaagattgcaggccttgagaggtgcgggaagagctgcaggctg 281
Query: 449 aggtggataaactatctgaggccagacctcaagagaggggcattcgcccaagacgaagaa 508
|||||||||||||| |||||||| ||||||||||| || || ||| | || || || ||
Sbjct: 282 aggtggataaactacctgaggccggacctcaagaggggcgccttctcgcaggaggaggag 341
Query: 509 gacctcatcat 519
|||||||||||
Sbjct: 342 gacctcatcat 352
>gb|BU998112.1|BU998112 HI10A14r HI Hordeum vulgare subsp. vulgare cDNA clone HI10A14
5-PRIME, mRNA sequence
Length = 580
Score = 81.8 bits (41), Expect = 3e-014
Identities = 149/185 (80%)
Strand = Plus / Plus
Query: 299 tgctgctacaagcagaagctgagaaaggggctctggtcccctgaggaggatgagaaactc 358
|||||| ||||||||||| |||| | ||| || ||||| || ||||| || ||||| |||
Sbjct: 76 tgctgcaacaagcagaaggtgaggaggggcctgtggtcaccagaggaagacgagaagctc 135
Query: 359 atgaatcacataacaaagcatggccatggctgctggagctctgttcccaaactcgctggg 418
| || |||| || |||||||||||||||||||||||| || || | || || |||
Sbjct: 136 gtcaagtacatcaccgcgcatggccatggctgctggagctcggtcccaagacaagccggg 195
Query: 419 cttcaaaggtgcggaaagagttgtaggctgaggtggataaactatctgaggccagacctc 478
|| || ||||| || ||||| || |||||| ||||||| ||||| |||||||| ||||||
Sbjct: 196 ctgcagaggtgtggcaagagctgcaggctgcggtggatcaactacctgaggccggacctc 255
Query: 479 aagag 483
|||||
Sbjct: 256 aagag 260
>gb|BQ463523.1|BQ463523 HI05J08r HI Hordeum vulgare subsp. vulgare cDNA clone HI05J08
5-PRIME, mRNA sequence
Length = 436
Score = 52.0 bits (26), Expect = 2e-005
Identities = 41/46 (89%)
Strand = Plus / Plus
Query: 309 agcagaagctgagaaaggggctctggtcccctgaggaggatgagaa 354
||||||||||| | |||||||||||||| || |||||||| |||||
Sbjct: 132 agcagaagctgcgcaaggggctctggtctcccgaggaggacgagaa 177
Score = 44.1 bits (22), Expect = 0.006
Identities = 61/74 (82%)
Strand = Plus / Plus
Query: 389 tgctggagctctgttcccaaactcgctgggcttcaaaggtgcggaaagagttgtaggctg 448
||||||||||| || ||||| ||||| || || || |||||||| ||||| || |||||
Sbjct: 212 tgctggagctccgtgcccaagctcgccggtctgcagaggtgcggcaagagctgccggctg 271
Query: 449 aggtggataaacta 462
||||||| |||||
Sbjct: 272 cggtggatcaacta 285
>gb|BI958189.1|BI958189 HVSMEn0013M24f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0013M24f, mRNA sequence
Length = 471
Score = 50.1 bits (25), Expect = 1e-004
Identities = 85/105 (80%)
Strand = Plus / Plus
Query: 377 catggccatggctgctggagctctgttcccaaactcgctgggcttcaaaggtgcggaaag 436
|||||| | ||||||||||| || | |||||| ||| ||||| | ||||||| |||
Sbjct: 256 catggcgagggctgctggaggtccctccccaaagccgcagggctgctgcggtgcgggaag 315
Query: 437 agttgtaggctgaggtggataaactatctgaggccagacctcaag 481
|| || |||||| ||||||| ||||| |||||||| |||||||||
Sbjct: 316 agctgcaggctgcggtggatcaactacctgaggccggacctcaag 360
>gb|BG343139.1|BG343139 HVSMEg0002L02f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0002L02f, mRNA sequence
Length = 823
Score = 38.2 bits (19), Expect = 0.37
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 18 ggagtgcccctagcctttc 36
|||||||||||||||||||
Sbjct: 591 ggagtgcccctagcctttc 609
>gb|CA022106.1|CA022106 HZ42C22r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ42C22
5-PRIME, mRNA sequence
Length = 530
Score = 38.2 bits (19), Expect = 0.37
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 345 aggatgagaaactcatgaa 363
|||||||||||||||||||
Sbjct: 394 aggatgagaaactcatgaa 376
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 64,985
Number of Sequences: 312970
Number of extensions: 64985
Number of successful extensions: 18720
Number of sequences better than 0.5: 8
Number of HSP's better than 0.5 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 18705
Number of HSP's gapped (non-prelim): 10
length of query: 687
length of database: 175,134,539
effective HSP length: 19
effective length of query: 668
effective length of database: 169,188,109
effective search space: 113017656812
effective search space used: 113017656812
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)