BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBJ23f06.xg.2.1
(683 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ465022.1|BQ465022 HU02G23r HU Hordeum vulgare subsp. v... 361 2e-098
gb|AL505620.1|AL505620 AL505620 Hordeum vulgare Barke roots... 52 2e-005
gb|BI948612.1|BI948612 HVSMEl0010E03f Hordeum vulgare spike... 50 1e-004
gb|BE559029.2|BE559029 HV_CEb0022G04f Hordeum vulgare seedl... 42 0.023
gb|BQ663389.1|BQ663389 HU02G23u HU Hordeum vulgare subsp. v... 42 0.023
gb|BF630220.2|BF630220 HVSMEb0008G15f Hordeum vulgare seedl... 38 0.37
>gb|BQ465022.1|BQ465022 HU02G23r HU Hordeum vulgare subsp. vulgare cDNA clone HU02G23
5-PRIME, mRNA sequence
Length = 700
Score = 361 bits (182), Expect = 2e-098
Identities = 350/407 (85%)
Strand = Plus / Plus
Query: 38 cggcgatggcgagcctcaccctgccgccggcgatggcgaacccacggcaggacgccatcg 97
|||| ||||||||||||||| ||||||||||| ||||||| || |||||||||||||
Sbjct: 294 cggcaatggcgagcctcaccttgccgccggcgcccccgaacccccgccaggacgccatcg 353
Query: 98 acctccaaaaggccttcaaagggtttggctgtgacagtacaacagtgataaacatactta 157
|||||||||||||||||||||||||||||||||| ||||||||||| ||| ||||||
Sbjct: 354 acctccaaaaggccttcaaagggtttggctgtgatagtacaacagtttcaaatatacttg 413
Query: 158 ctcatcgcgattcagtgcagcgtggactcattcaacaggaatacagggctatgtatcatg 217
|||| || || ||| |||| |||||| ||||||||||||||||| |||||||| |
Sbjct: 414 ctcaccgtgactcaatgcaacgtggatacattcaacaggaatacaaaactatgtattccg 473
Query: 218 aggaactctcccatcgcatttcatctgaactcaatggaaaccacaagaaagctatgttgc 277
||||||| || | ||| || || |||||||||| |||||||||||||||||| |||| ||
Sbjct: 474 aggaactttcgcgtcgtatatcgtctgaactcagtggaaaccacaagaaagcaatgtcgc 533
Query: 278 tgtggattcttgatcctgctggacgtgatncnactgttttgagagaagctctaagtggtg 337
| ||||||||||||||||||||||||||| | |||||| ||| ||||||||| |||| ||
Sbjct: 534 tctggattcttgatcctgctggacgtgatgcgactgttctgaaagaagctctcagtgctg 593
Query: 338 acactatggatctgagagcagccactgatattatatgttccaggacaccatcacagttgn 397
| | | | ||||||| |||||| |||||||| |||||||| ||||||||||||||| ||
Sbjct: 594 aaagtcttgatctgaaagcagctactgatataatatgttcgaggacaccatcacagctgc 653
Query: 398 aaataatgaaacagacttattatgctagatttggtacttatcttgag 444
|||||||||||||||| |||||||| | ||||||||||||||||||
Sbjct: 654 aaataatgaaacagacatattatgcaaagtttggtacttatcttgag 700
>gb|AL505620.1|AL505620 AL505620 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09A09V 5', mRNA sequence
Length = 468
Score = 52.0 bits (26), Expect = 2e-005
Identities = 49/57 (85%)
Strand = Plus / Plus
Query: 526 gaggttgatcctaccattgngacacatgatgcaaaggatttgtacaaagctggtgag 582
||||||||||| || || | |||||||||||| ||||| | |||||||||||||||
Sbjct: 16 gaggttgatcccactatagtgacacatgatgcgaaggacctctacaaagctggtgag 72
>gb|BI948612.1|BI948612 HVSMEl0010E03f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0010E03f, mRNA sequence
Length = 812
Score = 50.1 bits (25), Expect = 1e-004
Identities = 55/65 (84%)
Strand = Plus / Plus
Query: 88 gacgccatcgacctccaaaaggccttcaaagggtttggctgtgacagtacaacagtgata 147
|||||||||| |||||| | |||||||||||| ||||| || ||||| || || |||||
Sbjct: 95 gacgccatcgccctccacagggccttcaaaggttttggttgcgacagcacgacggtgatc 154
Query: 148 aacat 152
|||||
Sbjct: 155 aacat 159
>gb|BE559029.2|BE559029 HV_CEb0022G04f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0022G04f, mRNA sequence
Length = 669
Score = 42.1 bits (21), Expect = 0.023
Identities = 27/30 (90%)
Strand = Plus / Plus
Query: 622 ttcactgnacgcngctgggcacacntggca 651
||||||| |||| ||||||||||| |||||
Sbjct: 60 ttcactgaacgcagctgggcacacatggca 89
>gb|BQ663389.1|BQ663389 HU02G23u HU Hordeum vulgare subsp. vulgare cDNA clone HU02G23
3-PRIME, mRNA sequence
Length = 495
Score = 42.1 bits (21), Expect = 0.023
Identities = 27/30 (90%)
Strand = Plus / Minus
Query: 622 ttcactgnacgcngctgggcacacntggca 651
||||||| |||| ||||||||||| |||||
Sbjct: 479 ttcactgaacgcagctgggcacacatggca 450
>gb|BF630220.2|BF630220 HVSMEb0008G15f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0008G15f, mRNA sequence
Length = 499
Score = 38.2 bits (19), Expect = 0.37
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 58 ctgccgccggcgatggcga 76
|||||||||||||||||||
Sbjct: 118 ctgccgccggcgatggcga 100
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 55,530
Number of Sequences: 312970
Number of extensions: 55530
Number of successful extensions: 14414
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 14402
Number of HSP's gapped (non-prelim): 11
length of query: 683
length of database: 175,134,539
effective HSP length: 19
effective length of query: 664
effective length of database: 169,188,109
effective search space: 112340904376
effective search space used: 112340904376
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)