BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QAM10b11.yg.2.1
         (554 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AL508426.1|AL508426  AL508426 Hordeum vulgare Barke devel...    90   9e-017
gb|BF621295.3|BF621295  HVSMEa0006G07f Hordeum vulgare seedl...    38   0.30 

>gb|AL508426.1|AL508426 AL508426 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
           Hordeum vulgare subsp. vulgare cDNA clone HY08L15V 5',
           mRNA sequence
          Length = 700

 Score = 89.7 bits (45), Expect = 9e-017
 Identities = 78/89 (87%)
 Strand = Plus / Plus

                                                                       
Query: 419 gccgcgacgaacgtgtccacctggattggcagctgctacctcacgccgctcatcagcgcc 478
           ||||||| ||| |||||||||||| | |||||||||| |||||||||| ||||| | |||
Sbjct: 284 gccgcgaggaatgtgtccacctgggtcggcagctgcttcctcacgccggtcatcggagcc 343

                                        
Query: 479 ttcttggccgacacgtactggggaagata 507
           |||||||||||| | || |||||||||||
Sbjct: 344 ttcttggccgactcttattggggaagata 372




 Score = 61.9 bits (31), Expect = 2e-008
 Identities = 64/75 (85%)
 Strand = Plus / Plus

                                                                       
Query: 272 cagcgtgctctgaagaagagcaccgggaactggagagcatgcttcatgattctgggtacc 331
           |||| |||||| ||| |||||||||| |||||||||||||||||| | ||||| ||| ||
Sbjct: 137 cagcctgctctcaagcagagcaccggcaactggagagcatgcttcttcattctaggtgcc 196

                          
Query: 332 gagttcgccgaatgc 346
           || |||  |||||||
Sbjct: 197 gaattcagcgaatgc 211




 Score = 42.1 bits (21), Expect = 0.019
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                                
Query: 176 gcagatgaggagaggccactg 196
           |||||||||||||||||||||
Sbjct: 35  gcagatgaggagaggccactg 55



>gb|BF621295.3|BF621295 HVSMEa0006G07f Hordeum vulgare seedling shoot EST library
           HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
           cDNA clone HVSMEa0006G07f, mRNA sequence
          Length = 796

 Score = 38.2 bits (19), Expect = 0.30
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 523 cttcctcttgctctacatc 541
           |||||||||||||||||||
Sbjct: 202 cttcctcttgctctacatc 220



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 56,727
Number of Sequences: 312970
Number of extensions: 56727
Number of successful extensions: 16568
Number of sequences better than  0.5: 2
Number of HSP's better than  0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 16563
Number of HSP's gapped (non-prelim): 5
length of query: 554
length of database: 175,134,539
effective HSP length: 19
effective length of query: 535
effective length of database: 169,188,109
effective search space: 90515638315
effective search space used: 90515638315
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)