BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF41c07.yg.2.1
(691 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY177665.1| Hordeum vulgare subsp. vulgare putative call... 100 1e-019
gb|BU969103.1|BU969103 HB10J09r BC Hordeum vulgare subsp. v... 86 2e-015
gb|AV836635.1|AV836635 AV836635 K. Sato unpublished cDNA li... 78 4e-013
>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
complete cds
Length = 6180
Score = 99.6 bits (50), Expect = 1e-019
Identities = 283/360 (78%), Gaps = 3/360 (0%)
Strand = Plus / Minus
Query: 5 atctcggctgagaacttgctcaccattgcctccagcaactttcccctcaaatagtgcaat 64
||||| ||| |||||||| || || || ||||| |||||||| || ||||||||||| ||
Sbjct: 4493 atctctgctaagaacttgttcgccgtttcctcccgcaacttttccttcaaatagtgcgat 4434
Query: 65 ttggttcaagccaacatcacgccctttccccacctggatatattcatggtgtgtgatgtt 124
|| || | |||||||| | ||||| || ||||||||||| |||||||| || |||||
Sbjct: 4433 ctgattaagcccaacatctcttcctttaccaacctggatatactcatggtgagttatgtt 4374
Query: 125 cccttggcgcaaagtcgaattgaacccggcatatatatcttcgcttatattaatcactcg 184
|||||| |||| ||| ||||| ||||| ||| ||||||| || || ||||| || | ||
Sbjct: 4373 cccttgacgcagagtagaattaaaccctgcaaatatatcctcactgatattgatgatacg 4314
Query: 185 agacgccttgctaatgccacctcgggttatgtgaaatatccgatcaaaaacatcaggatg 244
|||||||| || |||||||| | || || |||||||| | |||||| || || || ||
Sbjct: 4313 ggacgccttactgatgccacccctcgtaatatgaaatattctatcaaacacgtctgggtg 4254
Query: 245 tccatagtgcattcgaactttga---ggtaagcaagaacacgttgccccaaagtaacaaa 301
||||| ||||||| ||||| | | | || ||||||||| || || || || |||||
Sbjct: 4253 accataatgcattctcactttcagtggattagaaagaacacgctgtcctaatgtcacaaa 4194
Query: 302 actagtctcctgtttcgacataaatgacgccagcgaagagacactccccgtgaatacatg 361
|||||| ||||| || ||||||||||| || || || || ||||| || ||||| |||||
Sbjct: 4193 actagtttcctgatttgacataaatgaagcaagggaggaaacacttccggtgaagacatg 4134
Score = 69.9 bits (35), Expect = 1e-010
Identities = 126/157 (80%)
Strand = Plus / Minus
Query: 420 aattcttcaagtaaatttctcattttcattgcttcctccaagtagttatcctgattcata 479
||||| ||||| | ||||| |||||| | ||| ||||| || ||||| ||||||||||||
Sbjct: 4084 aattcctcaagcagatttcgcattttgagtgcctcctcgaaatagttgtcctgattcata 4025
Query: 480 tcgatcgtctgaactgcatctcctcgagtgaagattatggcatggttttggttctcaggt 539
|| || || || || |||| ||| |||||||| |||| ||||||||||| || || |||
Sbjct: 4024 tcaatggtttgtacagcatttccgcgagtgaatattacagcatggttttgattttcgggt 3965
Query: 540 ntgccttcaccnagcttggggttcccaggcaatttaa 576
| || || || ||||| || || |||||||||||||
Sbjct: 3964 ttaccctccccaagctttggattgccaggcaatttaa 3928
>gb|BU969103.1|BU969103 HB10J09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10J09
5-PRIME, mRNA sequence
Length = 426
Score = 85.7 bits (43), Expect = 2e-015
Identities = 130/159 (81%)
Strand = Plus / Minus
Query: 5 atctcggctgagaacttgctcaccattgcctccagcaactttcccctcaaatagtgcaat 64
||||| ||| |||||||| || || || ||||| |||||||| || ||||||||||| ||
Sbjct: 181 atctctgctaagaacttgttcgccgtttcctcccgcaacttttccttcaaatagtgcgat 122
Query: 65 ttggttcaagccaacatcacgccctttccccacctggatatattcatggtgtgtgatgtt 124
|| || | |||||||| | ||||| || ||||||||||| |||||||| || |||||
Sbjct: 121 ctgattaagcccaacatctcttcctttaccaacctggatatactcatggtgagttatgtt 62
Query: 125 cccttggcgcaaagtcgaattgaacccggcatatatatc 163
|||||| |||| ||| ||||| ||||| ||| |||||||
Sbjct: 61 cccttgacgcagagtagaattaaaccctgcaaatatatc 23
>gb|AV836635.1|AV836635 AV836635 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd19k01, mRNA
sequence
Length = 644
Score = 77.8 bits (39), Expect = 4e-013
Identities = 120/147 (81%)
Strand = Plus / Minus
Query: 5 atctcggctgagaacttgctcaccattgcctccagcaactttcccctcaaatagtgcaat 64
||||| ||| |||||||| || || || ||||| |||||||| || ||||||||||| ||
Sbjct: 151 atctctgctaagaacttgttcgccgtttcctcccgcaacttttccttcaaatagtgcgat 92
Query: 65 ttggttcaagccaacatcacgccctttccccacctggatatattcatggtgtgtgatgtt 124
|| || | |||||||| | ||||| || ||||||||||| |||||||| || |||||
Sbjct: 91 ctgattaagcccaacatctcttcctttaccaacctggatatactcatggtgagttatgtt 32
Query: 125 cccttggcgcaaagtcgaattgaaccc 151
|||||| |||| ||| ||||| |||||
Sbjct: 31 cccttgacgcagagtagaattaaaccc 5
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 47,676
Number of Sequences: 312970
Number of extensions: 47676
Number of successful extensions: 11780
Number of sequences better than 0.5: 3
Number of HSP's better than 0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 11775
Number of HSP's gapped (non-prelim): 5
length of query: 691
length of database: 175,134,539
effective HSP length: 19
effective length of query: 672
effective length of database: 169,188,109
effective search space: 113694409248
effective search space used: 113694409248
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)