BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF1g01.yg.2.1
(335 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU969103.1|BU969103 HB10J09r BC Hordeum vulgare subsp. v... 86 8e-016
gb|AY177665.1| Hordeum vulgare subsp. vulgare putative call... 86 8e-016
gb|AV836635.1|AV836635 AV836635 K. Sato unpublished cDNA li... 78 2e-013
>gb|BU969103.1|BU969103 HB10J09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10J09
5-PRIME, mRNA sequence
Length = 426
Score = 85.7 bits (43), Expect = 8e-016
Identities = 130/159 (81%)
Strand = Plus / Minus
Query: 5 atctcggctgagaacttgctcaccattgcctccagcaactttcccctcaaatagtgcaat 64
||||| ||| |||||||| || || || ||||| |||||||| || ||||||||||| ||
Sbjct: 181 atctctgctaagaacttgttcgccgtttcctcccgcaacttttccttcaaatagtgcgat 122
Query: 65 ttggttcaagccaacatcacgccctttccccacctggatatattcatggtgtgtgatgtt 124
|| || | |||||||| | ||||| || ||||||||||| |||||||| || |||||
Sbjct: 121 ctgattaagcccaacatctcttcctttaccaacctggatatactcatggtgagttatgtt 62
Query: 125 cccttggcgcaaagtcgaattgaacccggcatatatatc 163
|||||| |||| ||| ||||| ||||| ||| |||||||
Sbjct: 61 cccttgacgcagagtagaattaaaccctgcaaatatatc 23
>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
complete cds
Length = 6180
Score = 85.7 bits (43), Expect = 8e-016
Identities = 130/159 (81%)
Strand = Plus / Minus
Query: 5 atctcggctgagaacttgctcaccattgcctccagcaactttcccctcaaatagtgcaat 64
||||| ||| |||||||| || || || ||||| |||||||| || ||||||||||| ||
Sbjct: 4493 atctctgctaagaacttgttcgccgtttcctcccgcaacttttccttcaaatagtgcgat 4434
Query: 65 ttggttcaagccaacatcacgccctttccccacctggatatattcatggtgtgtgatgtt 124
|| || | |||||||| | ||||| || ||||||||||| |||||||| || |||||
Sbjct: 4433 ctgattaagcccaacatctcttcctttaccaacctggatatactcatggtgagttatgtt 4374
Query: 125 cccttggcgcaaagtcgaattgaacccggcatatatatc 163
|||||| |||| ||| ||||| ||||| ||| |||||||
Sbjct: 4373 cccttgacgcagagtagaattaaaccctgcaaatatatc 4335
>gb|AV836635.1|AV836635 AV836635 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd19k01, mRNA
sequence
Length = 644
Score = 77.8 bits (39), Expect = 2e-013
Identities = 120/147 (81%)
Strand = Plus / Minus
Query: 5 atctcggctgagaacttgctcaccattgcctccagcaactttcccctcaaatagtgcaat 64
||||| ||| |||||||| || || || ||||| |||||||| || ||||||||||| ||
Sbjct: 151 atctctgctaagaacttgttcgccgtttcctcccgcaacttttccttcaaatagtgcgat 92
Query: 65 ttggttcaagccaacatcacgccctttccccacctggatatattcatggtgtgtgatgtt 124
|| || | |||||||| | ||||| || ||||||||||| |||||||| || |||||
Sbjct: 91 ctgattaagcccaacatctcttcctttaccaacctggatatactcatggtgagttatgtt 32
Query: 125 cccttggcgcaaagtcgaattgaaccc 151
|||||| |||| ||| ||||| |||||
Sbjct: 31 cccttgacgcagagtagaattaaaccc 5
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 19,298
Number of Sequences: 312970
Number of extensions: 19298
Number of successful extensions: 4149
Number of sequences better than 0.5: 3
Number of HSP's better than 0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 4146
Number of HSP's gapped (non-prelim): 3
length of query: 335
length of database: 175,134,539
effective HSP length: 18
effective length of query: 317
effective length of database: 169,501,079
effective search space: 53731842043
effective search space used: 53731842043
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)