BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAE8g03.yg.2.1
(360 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU994139.1|BU994139 HM05O02r HM Hordeum vulgare subsp. v... 82 1e-014
gb|CB880803.1|CB880803 HM07O02w HM Hordeum vulgare subsp. v... 82 1e-014
gb|BM442070.2|BM442070 EBan01_SQ002_B15_R anther, yellow st... 78 2e-013
gb|BM442430.2|BM442430 EBan01_SQ003_C15_R anther, yellow st... 76 9e-013
>gb|BU994139.1|BU994139 HM05O02r HM Hordeum vulgare subsp. vulgare cDNA clone HM05O02
5-PRIME, mRNA sequence
Length = 593
Score = 81.8 bits (41), Expect = 1e-014
Identities = 92/109 (84%)
Strand = Plus / Minus
Query: 108 gtcttgtgtaacccagatagcatctcctcatgatcggtgtcgccatgttccccagtgatc 167
|||||||||| || || ||||||||||| |||||||||||||||| |||||| ||||
Sbjct: 555 gtcttgtgtagtcctgaaagcatctcctcgcgatcggtgtcgccatgctccccaacgatc 496
Query: 168 agatacatgttccccacatataggccccagcgcacaaaaagatacctga 216
|| |||||||||||||| | ||||||||||||||| || |||||||
Sbjct: 495 aggtacatgttccccacggccaagccccagcgcacaaagaggtacctga 447
Score = 69.9 bits (35), Expect = 5e-011
Identities = 59/67 (88%)
Strand = Plus / Minus
Query: 294 tacctgagtgcctgtgaccttgatgctagcagagggacaacctgaagccttgtcgagttc 353
||||||||||| |||||||||||||| | |||||||||||||| | ||| |||||||||
Sbjct: 453 tacctgagtgcttgtgaccttgatgccatgagagggacaacctgcatcctcgtcgagttc 394
Query: 354 ctgcagt 360
| |||||
Sbjct: 393 cggcagt 387
>gb|CB880803.1|CB880803 HM07O02w HM Hordeum vulgare subsp. vulgare cDNA clone HM07O02
3-PRIME, mRNA sequence
Length = 626
Score = 81.8 bits (41), Expect = 1e-014
Identities = 92/109 (84%)
Strand = Plus / Plus
Query: 108 gtcttgtgtaacccagatagcatctcctcatgatcggtgtcgccatgttccccagtgatc 167
|||||||||| || || ||||||||||| |||||||||||||||| |||||| ||||
Sbjct: 421 gtcttgtgtagtcctgaaagcatctcctcgcgatcggtgtcgccatgctccccaacgatc 480
Query: 168 agatacatgttccccacatataggccccagcgcacaaaaagatacctga 216
|| |||||||||||||| | ||||||||||||||| || |||||||
Sbjct: 481 aggtacatgttccccacggccaagccccagcgcacaaagaggtacctga 529
Score = 69.9 bits (35), Expect = 5e-011
Identities = 59/67 (88%)
Strand = Plus / Plus
Query: 294 tacctgagtgcctgtgaccttgatgctagcagagggacaacctgaagccttgtcgagttc 353
||||||||||| |||||||||||||| | |||||||||||||| | ||| |||||||||
Sbjct: 523 tacctgagtgcttgtgaccttgatgccatgagagggacaacctgcatcctcgtcgagttc 582
Query: 354 ctgcagt 360
| |||||
Sbjct: 583 cggcagt 589
>gb|BM442070.2|BM442070 EBan01_SQ002_B15_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_B15 5', mRNA sequence
Length = 260
Score = 77.8 bits (39), Expect = 2e-013
Identities = 78/91 (85%)
Strand = Plus / Minus
Query: 126 agcatctcctcatgatcggtgtcgccatgttccccagtgatcagatacatgttccccaca 185
||||||||||| |||||||||||||||| |||||| |||||| ||||||||||||||
Sbjct: 116 agcatctcctcgcgatcggtgtcgccatgctccccaacgatcaggtacatgttccccacg 57
Query: 186 tataggccccagcgcacaaaaagatacctga 216
| ||||||||||||||| || |||||||
Sbjct: 56 gccaagccccagcgcacaaagaggtacctga 26
>gb|BM442430.2|BM442430 EBan01_SQ003_C15_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_C15 5', mRNA sequence
Length = 504
Score = 75.8 bits (38), Expect = 9e-013
Identities = 83/98 (84%)
Strand = Plus / Minus
Query: 108 gtcttgtgtaacccagatagcatctcctcatgatcggtgtcgccatgttccccagtgatc 167
|||||||||| || || ||||||||||| |||||||||||||||| |||||| ||||
Sbjct: 356 gtcttgtgtagtcctgaaagcatctcctcgcgatcggtgtcgccatgctccccaacgatc 297
Query: 168 agatacatgttccccacatataggccccagcgcacaaa 205
|| |||||||||||||| | |||||||||||||||
Sbjct: 296 aggtacatgttccccacggccaagccccagcgcacaaa 259
Score = 61.9 bits (31), Expect = 1e-008
Identities = 55/63 (87%)
Strand = Plus / Minus
Query: 298 tgagtgcctgtgaccttgatgctagcagagggacaacctgaagccttgtcgagttcctgc 357
||||||| |||||||||||||| | |||||||||||||| | ||| |||||||||| ||
Sbjct: 250 tgagtgcttgtgaccttgatgccatgagagggacaacctgcatcctcgtcgagttccggc 191
Query: 358 agt 360
|||
Sbjct: 190 agt 188
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 32,132
Number of Sequences: 312970
Number of extensions: 32132
Number of successful extensions: 8018
Number of sequences better than 0.5: 4
Number of HSP's better than 0.5 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 8006
Number of HSP's gapped (non-prelim): 8
length of query: 360
length of database: 175,134,539
effective HSP length: 18
effective length of query: 342
effective length of database: 169,501,079
effective search space: 57969369018
effective search space used: 57969369018
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)