BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8161196.3.1
(825 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ458955.1|BQ458955 HA02L20r HA Hordeum vulgare subsp. v... 167 7e-040
gb|BE558327.2|BE558327 HV_CEb0017D19f Hordeum vulgare seedl... 139 2e-031
gb|BF631488.2|BF631488 HVSMEb0016C05f Hordeum vulgare seedl... 137 6e-031
gb|BI950228.1|BI950228 HVSMEl0019K24f Hordeum vulgare spike... 125 2e-027
gb|BF628377.3|BF628377 HVSMEb0003N23f Hordeum vulgare seedl... 92 3e-017
gb|AV833264.1|AV833264 AV833264 K. Sato unpublished cDNA li... 76 2e-012
gb|BE196144.3|BE196144 HVSMEh0091H18f Hordeum vulgare 5-45 ... 40 0.11
gb|BI952411.1|BI952411 HVSMEm0006F13f Hordeum vulgare green... 38 0.44
>gb|BQ458955.1|BQ458955 HA02L20r HA Hordeum vulgare subsp. vulgare cDNA clone HA02L20
5-PRIME, mRNA sequence
Length = 591
Score = 167 bits (84), Expect = 7e-040
Identities = 267/328 (81%)
Strand = Plus / Plus
Query: 358 ccaggatcatggttcctctctgtggtttctgtgggaaacaaaggtcaatgatctactgca 417
|||||||||||| | |||| |||| ||| || ||| | |||||| ||| || || |||
Sbjct: 238 ccaggatcatgggtgctctttgtgatttttgcggggagcaaaggccaacaatttattgcc 297
Query: 418 gatcggatgcggcatcattgtgcttatcatgcgaccgtagcgttcattcagctaatgcac 477
|||| ||||| || || | |||||||| |||||||||| || ||||| |||||| | |
Sbjct: 298 gatcagatgccgcgtccctatgcttatcgtgcgaccgtaatgtgcattcggctaattccc 357
Query: 478 tgtctcggcgtcatagaaggacccttctttgtgatcgttgtggtttacagcctgcatcag 537
| ||||| || |||| ||||||||||||||||||||||||| || ||||||||| |||
Sbjct: 358 tttctcgacgacatatgaggacccttctttgtgatcgttgtgcttcacagcctgcggcag 417
Query: 538 tccgatgtcttgaggacaacacatcactttgccaaaactgtgattggaatgggcatgatg 597
||||||||||||| || |||| |||||||||||||||||||||||||| ||||||| |
Sbjct: 418 tccgatgtcttgaagagaacatttcactttgccaaaactgtgattggaacgggcatggcg 477
Query: 598 cagcatcaggggcttctgggcataaaaggcaggccataaactgttactcagggtgcccat 657
| |||| |||| | |||||| ||||||| ||||||| || ||||| ||||||||||
Sbjct: 478 cgacatccttggctgccgggcatctaaggcagaccataaattgctactcggggtgcccat 537
Query: 658 catcagcagagctttcgagaatctggtc 685
|||||| ||||| | |||||||||||
Sbjct: 538 catcagaggagctggcaagaatctggtc 565
>gb|BE558327.2|BE558327 HV_CEb0017D19f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0017D19f, mRNA sequence
Length = 800
Score = 139 bits (70), Expect = 2e-031
Identities = 262/326 (80%)
Strand = Plus / Plus
Query: 363 atcatggttcctctctgtggtttctgtgggaaacaaaggtcaatgatctactgcagatcg 422
||||||| | ||||||| | || ||||||| | || ||||| ||| | |||||| ||||
Sbjct: 310 atcatgggtgctctctgcgattactgtggggagcataggtccatggtttactgccgatcc 369
Query: 423 gatgcggcatcattgtgcttatcatgcgaccgtagcgttcattcagctaatgcactgtct 482
||||| || || |||||||| ||||||||||| | || |||||||||||||| || |||
Sbjct: 370 gatgcagcctctttgtgcttgtcatgcgaccgcaatgtgcattcagctaatgctctttct 429
Query: 483 cggcgtcatagaaggacccttctttgtgatcgttgtggtttacagcctgcatcagtccga 542
||||| |||| ||| |||||||| ||||| || |||| || ||||||||| |||||
Sbjct: 430 cggcgccatacaagaacccttctatgtgaccggtgtgcttcacagcctgccatggtccgc 489
Query: 543 tgtcttgaggacaacacatcactttgccaaaactgtgattggaatgggcatgatgcagca 602
|| || || || ||||| |||||||||||||| ||||| |||||||||||| ||| | |
Sbjct: 490 tgcctagaagagaacacctcactttgccaaaattgtgactggaatgggcatagtgctgga 549
Query: 603 tcaggggcttctgggcataaaaggcaggccataaactgttactcagggtgcccatcatca 662
|| ||| |||| || ||||| ||| |||||||| || || ||||||||||||||
Sbjct: 550 tcctccgctgctggacacaaaagacagaatataaactgctattcggggtgcccatcatct 609
Query: 663 gcagagctttcgagaatctggtcatt 688
||||||||||| ||||| ||||||||
Sbjct: 610 gcagagctttcaagaatgtggtcatt 635
>gb|BF631488.2|BF631488 HVSMEb0016C05f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0016C05f, mRNA sequence
Length = 788
Score = 137 bits (69), Expect = 6e-031
Identities = 156/185 (84%)
Strand = Plus / Plus
Query: 501 cttctttgtgatcgttgtggtttacagcctgcatcagtccgatgtcttgaggacaacaca 560
||||||||||||||||||| || ||||||||| |||||||||||||||| || ||||
Sbjct: 1 cttctttgtgatcgttgtgcttcacagcctgcggcagtccgatgtcttgaagagaacatt 60
Query: 561 tcactttgccaaaactgtgattggaatgggcatgatgcagcatcaggggcttctgggcat 620
|||||||||||||||||||||||||| ||||||| || |||| |||| | ||||||
Sbjct: 61 tcactttgccaaaactgtgattggaacgggcatggcgcgacatccttggctgccgggcat 120
Query: 621 aaaaggcaggccataaactgttactcagggtgcccatcatcagcagagctttcgagaatc 680
||||||| ||||||| || ||||| |||||||||||||||| ||||| | ||||||
Sbjct: 121 ctaaggcagaccataaattgctactcggggtgcccatcatcagaggagctggcaagaatc 180
Query: 681 tggtc 685
|||||
Sbjct: 181 tggtc 185
>gb|BI950228.1|BI950228 HVSMEl0019K24f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0019K24f, mRNA sequence
Length = 572
Score = 125 bits (63), Expect = 2e-027
Identities = 260/326 (79%)
Strand = Plus / Plus
Query: 363 atcatggttcctctctgtggtttctgtgggaaacaaaggtcaatgatctactgcagatcg 422
||||||| | ||||||| | || ||||||| | || ||||| ||| | |||||| ||||
Sbjct: 237 atcatgggtgctctctgcgattactgtggggagcataggtccatggtttactgccgatcc 296
Query: 423 gatgcggcatcattgtgcttatcatgcgaccgtagcgttcattcagctaatgcactgtct 482
||||| | || |||||||| ||||||||||| | || |||||||||||||| || |||
Sbjct: 297 gatgcancctctttgtgcttgtcatgcgaccgcaatgtgcattcagctaatgctctttct 356
Query: 483 cggcgtcatagaaggacccttctttgtgatcgttgtggtttacagcctgcatcagtccga 542
||||| |||| ||| |||||||| ||||| || |||| || ||||||||| |||||
Sbjct: 357 cggcgccatacaagaacccttctatgtgaccggtgtgcttcacagcctgccatggtccgc 416
Query: 543 tgtcttgaggacaacacatcactttgccaaaactgtgattggaatgggcatgatgcagca 602
|| || | || ||||| |||||||||||||| ||||| |||||||||||| ||| | |
Sbjct: 417 tgcctaaaagagaacacctcactttgccaaaattgtgactggaatgggcatagtgctgga 476
Query: 603 tcaggggcttctgggcataaaaggcaggccataaactgttactcagggtgcccatcatca 662
|| ||| |||| || ||||| ||| |||||||| || || ||||||||||||||
Sbjct: 477 tcctccgctgctggacacaaaagacagaatataaactgctattcggggtgcccatcatct 536
Query: 663 gcagagctttcgagaatctggtcatt 688
||||||||||| ||||| ||||||||
Sbjct: 537 gcagagctttcaagaatgtggtcatt 562
>gb|BF628377.3|BF628377 HVSMEb0003N23f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0003N23f, mRNA sequence
Length = 516
Score = 91.7 bits (46), Expect = 3e-017
Identities = 139/170 (81%)
Strand = Plus / Plus
Query: 363 atcatggttcctctctgtggtttctgtgggaaacaaaggtcaatgatctactgcagatcg 422
||||||| | ||||||| | || ||||||| | || ||||| ||| | |||||| ||||
Sbjct: 342 atcatgggtgctctctgcgattactgtggggagcataggtccatggtttactgccgatcc 401
Query: 423 gatgcggcatcattgtgcttatcatgcgaccgtagcgttcattcagctaatgcactgtct 482
||||| || || |||||||| ||||||||||| | || |||||||||||||| || |||
Sbjct: 402 gatgcagcctctttgtgcttgtcatgcgaccgcaatgtgcattcagctaatgctctttct 461
Query: 483 cggcgtcatagaaggacccttctttgtgatcgttgtggtttacagcctgc 532
||||| |||| ||| |||||||| ||||| || |||| || |||||||||
Sbjct: 462 cggcgccatacaagaacccttctatgtgaccggtgtgcttcacagcctgc 511
>gb|AV833264.1|AV833264 AV833264 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags4i11, mRNA sequence
Length = 508
Score = 75.8 bits (38), Expect = 2e-012
Identities = 110/134 (82%)
Strand = Plus / Plus
Query: 555 aacacatcactttgccaaaactgtgattggaatgggcatgatgcagcatcaggggcttct 614
||||| |||||||||||||| ||||| |||||||||||| ||| | ||| ||| ||
Sbjct: 29 aacacctcactttgccaaaattgtgactggaatgggcatagtgctggatcctccgctgct 88
Query: 615 gggcataaaaggcaggccataaactgttactcagggtgcccatcatcagcagagctttcg 674
|| || ||||| ||| |||||||| || || |||||||||||||| |||||||||||
Sbjct: 89 ggacacaaaagacagaatataaactgctattcggggtgcccatcatctgcagagctttca 148
Query: 675 agaatctggtcatt 688
||||| ||||||||
Sbjct: 149 agaatgtggtcatt 162
>gb|BE196144.3|BE196144 HVSMEh0091H18f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0091H18f, mRNA sequence
Length = 894
Score = 40.1 bits (20), Expect = 0.11
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 17 caccaccaccacaccacgcc 36
||||||||||||||||||||
Sbjct: 509 caccaccaccacaccacgcc 490
>gb|BI952411.1|BI952411 HVSMEm0006F13f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0006F13f, mRNA sequence
Length = 780
Score = 38.2 bits (19), Expect = 0.44
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 376 tctgtggtttctgtgggaa 394
|||||||||||||||||||
Sbjct: 626 tctgtggtttctgtgggaa 644
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 84,943
Number of Sequences: 312970
Number of extensions: 84943
Number of successful extensions: 25208
Number of sequences better than 0.5: 8
Number of HSP's better than 0.5 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 25187
Number of HSP's gapped (non-prelim): 20
length of query: 825
length of database: 175,134,539
effective HSP length: 19
effective length of query: 806
effective length of database: 169,188,109
effective search space: 136365615854
effective search space used: 136365615854
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)