BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5705822.3.1
(888 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG343555.1|BG343555 HVSMEg0006G02f Hordeum vulgare pre-a... 688 0.0
gb|BE603226.3|BE603226 HVSMEh0102J20f Hordeum vulgare 5-45 ... 587 e-166
gb|CA018069.1|CA018069 HV07F22r HV Hordeum vulgare subsp. v... 434 e-120
gb|BI952691.1|BI952691 HVSMEm0007G14f Hordeum vulgare green... 373 e-102
gb|BG416017.1|BG416017 HVSMEk0009H03f Hordeum vulgare testa... 365 2e-099
gb|BI949337.1|BI949337 HVSMEl0013G14f Hordeum vulgare spike... 357 4e-097
gb|BQ464262.1|BQ464262 HF01M14T HF Hordeum vulgare subsp. v... 232 2e-059
gb|BF255585.2|BF255585 HVSMEf0007E04f Hordeum vulgare seedl... 206 9e-052
gb|BU974636.1|BU974636 HB28J16r BC Hordeum vulgare subsp. v... 50 1e-004
gb|BQ471200.1|BQ471200 HV01J02T HV Hordeum vulgare subsp. v... 42 0.031
>gb|BG343555.1|BG343555 HVSMEg0006G02f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0006G02f, mRNA sequence
Length = 954
Score = 688 bits (347), Expect = 0.0
Identities = 598/680 (87%), Gaps = 2/680 (0%)
Strand = Plus / Plus
Query: 76 agaacgtgctggattcgctcatcccggcgcttctcaaggacgagaaccgaaagttcatct 135
|||||||||||||||||||| | |||||||| |||| |||||||||||| ||||||||||
Sbjct: 1 agaacgtgctggattcgctcgtgccggcgctgctcagggacgagaaccgcaagttcatct 60
Query: 136 atgttgagcaggcattcttccaaagatggtggaggaaccagaatgacatgatcaaggata 195
| || ||||||||||| ||||| || ||||||||| | |||| ||||| ||||||||||
Sbjct: 61 acgtcgagcaggcatttttccagaggtggtggaggcagcagagcgacattatcaaggata 120
Query: 196 cagtgaaggggctcatcagctctggacggctggaattaataaatggtggcatgtgcatgc 255
| |||||||||||| ||||||| || ||||||||||| |||||||| || ||||||||||
Sbjct: 121 cggtgaaggggctcgtcagctccgggcggctggaattcataaatgggggtatgtgcatgc 180
Query: 256 acgatgaggcggctgtacactatattgacatgatcgatcagactacacttggacacaagt 315
|||| |||||| | || ||||| ||||||||||| ||||||||||| || || |||| ||
Sbjct: 181 acgacgaggcgaccgtgcactacattgacatgattgatcagactacgctgggccacaggt 240
Query: 316 acatcaaggaggagtttggccagatcccacggattggctggcagattgatccgtttgggc 375
|||||||||||||||||| ||||| ||| ||||||| |||||||| |||||||||||||
Sbjct: 241 tcatcaaggaggagtttggtcagattccaaggattggttggcagatagatccgtttgggc 300
Query: 376 actctgcggttcaggcttaccttcttggcgcagaagtaggattcgatgctttctatttct 435
||||||| |||||||||||||| || || |||||||||||||| ||||| || ||||| |
Sbjct: 301 actctgcagttcaggcttacctcctcggtgcagaagtaggatttgatgcattatattttt 360
Query: 436 tccggattgattaccaagaccgtgatacacggaaaggaacaaaagaactcgaggttgtat 495
||||||||||||||||||| ||||| ||| |||| || ||||||||||||||||||||||
Sbjct: 361 tccggattgattaccaagatcgtgacacaaggaaggggacaaaagaactcgaggttgtat 420
Query: 496 ggaggggctccaagaccttgggctcatcggctgatatctttgctggcatctttccaaaaa 555
||||||||||||||||||| |||||||| |||||||| |||||||||||||| |||||||
Sbjct: 421 ggaggggctccaagacctttggctcatcagctgatatttttgctggcatcttcccaaaaa 480
Query: 556 actatgaaccacctcctggtgatttttattttgaagttgatgataattcccctgttgtcc 615
| ||||||||||| |||||||| |||||||||||||| |||| || ||||||||||||||
Sbjct: 481 attatgaaccaccacctggtgacttttattttgaagtcgatgctacttcccctgttgtcc 540
Query: 616 aggatgatccccttcttttcgattataatgttgaagaacgagtaaatgattttgttgctg 675
||||||||||||||||||| ||||| || |||||| |||| || ||||||| || |||||
Sbjct: 541 aggatgatccccttctttttgattacaacgttgaacaacgggtgaatgattntgntgctg 600
Query: 676 cagctgtagcacaggcaaatatcacgaggacaaatcatatcatgtttacaatggg-aaca 734
|||| |||||||||||| | || ||||||| ||| |||||||||||||||| ||||
Sbjct: 601 cagcgcaggcacaggcaaatgttacanggacaaaccatgtcatgtttacaatgggaaaca 660
Query: 735 gacttcaaataccaatacgc 754
||| ||||||| ||||||||
Sbjct: 661 gac-tcaaatatcaatacgc 679
>gb|BE603226.3|BE603226 HVSMEh0102J20f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0102J20f, mRNA sequence
Length = 528
Score = 587 bits (296), Expect = e-166
Identities = 446/496 (89%)
Strand = Plus / Plus
Query: 317 catcaaggaggagtttggccagatcccacggattggctggcagattgatccgtttgggca 376
|||||||||||||||||| ||||| ||| ||||||| |||||||| ||||||||||||||
Sbjct: 19 catcaaggaggagtttggtcagattccaaggattggttggcagatagatccgtttgggca 78
Query: 377 ctctgcggttcaggcttaccttcttggcgcagaagtaggattcgatgctttctatttctt 436
|||||| |||||||||||||| || || |||||||||||||| ||||| || ||||| ||
Sbjct: 79 ctctgcagttcaggcttacctcctcggtgcagaagtaggatttgatgcattatatttttt 138
Query: 437 ccggattgattaccaagaccgtgatacacggaaaggaacaaaagaactcgaggttgtatg 496
|||||||||||||||||| ||||| ||| |||| || |||||||||||||||||||||||
Sbjct: 139 ccggattgattaccaagatcgtgacacaaggaaggggacaaaagaactcgaggttgtatg 198
Query: 497 gaggggctccaagaccttgggctcatcggctgatatctttgctggcatctttccaaaaaa 556
|||||||||||||||||| |||||||| |||||||| |||||||||||||| ||||||||
Sbjct: 199 gaggggctccaagacctttggctcatcagctgatatttttgctggcatcttcccaaaaaa 258
Query: 557 ctatgaaccacctcctggtgatttttattttgaagttgatgataattcccctgttgtcca 616
||||||||||| |||||||| |||||||||||||| |||| || |||||||||||||||
Sbjct: 259 ttatgaaccaccacctggtgacttttattttgaagtcgatgctacttcccctgttgtcca 318
Query: 617 ggatgatccccttcttttcgattataatgttgaagaacgagtaaatgattttgttgctgc 676
|||||||||||||||||| ||||| || |||||| |||| || |||||||||||||||||
Sbjct: 319 ggatgatccccttctttttgattacaacgttgaacaacgggtgaatgattttgttgctgc 378
Query: 677 agctgtagcacaggcaaatatcacgaggacaaatcatatcatgtttacaatgggaacaga 736
||| | |||||||||||| | || |||||||| ||| ||||||||||||||||||||||
Sbjct: 379 agcgctggcacaggcaaatgttacaaggacaaaccatgtcatgtttacaatgggaacaga 438
Query: 737 cttcaaataccaatacgcagagagttggttcggaaatatggacaaactaattcactatgt 796
||||||||| |||||||| || ||||||||| || | ||||||||| ||||||| |||||
Sbjct: 439 cttcaaatatcaatacgcggaaagttggttcagacagatggacaaattaattcattatgt 498
Query: 797 gaacaaggatggccgt 812
||||||||||||||||
Sbjct: 499 gaacaaggatggccgt 514
>gb|CA018069.1|CA018069 HV07F22r HV Hordeum vulgare subsp. vulgare cDNA clone HV07F22
5-PRIME, mRNA sequence
Length = 497
Score = 434 bits (219), Expect = e-120
Identities = 423/492 (85%), Gaps = 3/492 (0%)
Strand = Plus / Plus
Query: 206 gctcatcagctctggacggctggaattaataaatggtggcatgtgcatgcacgatgaggc 265
|||| ||||||| || |||||||||||| ||||||| || |||||||||||||| |||||
Sbjct: 2 gctcgtcagctccgggcggctggaatta-taaatgggggtatgtgcatgcacgacgaggc 60
Query: 266 ggctgtacactatattgacatgatcgatcagactacacttggacacaagtacatcaagga 325
| | || ||||| || ||||||| ||||||||||| || || |||| || |||||||||
Sbjct: 61 gaccgtgcactacatnnacatgattgatcagactacgctgggccacaggttcatcaagga 120
Query: 326 ggagtttggccagatcccacggattggctggcagattgatccgtttgggcactctgcggt 385
||||||||| ||||| ||| ||||||| |||||||| ||||||||||||||||||| ||
Sbjct: 121 ggagtttggtcagattccaaggattggttggcagatnnatccgtttgggcactctgcagt 180
Query: 386 tcaggcttaccttcttggcgcagaagtaggattcgatgctttctatt-tcttccggattg 444
|||||||||||| || || |||||||||||||| ||||| || || | ||||||||||
Sbjct: 181 tcaggcttacctcctcggtgcagaagtaggatttgatgcattatanncttttccggattg 240
Query: 445 attaccaagaccgtgatacacggaaaggaacaaaagaactcgaggttgtatggaggggct 504
|||||||||| ||||| ||| |||| || |||||||||||||||||||||| |||||||
Sbjct: 241 attaccaagatcgtgacacaaggaaggggacaaaagaactcgaggttgtatnnaggggct 300
Query: 505 ccaagaccttgggctcatcggctgatatctttgctggcatctttccaaaaaactatgaac 564
|||||||||| |||||||| |||||||| |||||||||||||| |||||||| |||||||
Sbjct: 301 ccaagacctttggctcatcagctgatatttttgctggcatcttcccaaaaaattatgaac 360
Query: 565 cacctc-ctggtgatttttattttgaagttgatgataattcccctgttgtccaggatgat 623
|||| | |||| |||||||||||||| |||| || ||||||||||||||||||||||
Sbjct: 361 caccacnnnngtgacttttattttgaagtcgatgctacttcccctgttgtccaggatgat 420
Query: 624 ccccttcttttcgattataatgttgaagaacgagtaaatgattttgttgctgcagctgta 683
||||||||||| ||||| | |||| |||| || |||||||||||||||||||| |
Sbjct: 421 ccccttctttttgattacannnntgaacaacgggtgaatgattttgttgctgcagcgctg 480
Query: 684 gcacaggcaaat 695
||||||||||||
Sbjct: 481 gcacaggcaaat 492
>gb|BI952691.1|BI952691 HVSMEm0007G14f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0007G14f, mRNA sequence
Length = 430
Score = 373 bits (188), Expect = e-102
Identities = 302/340 (88%)
Strand = Plus / Plus
Query: 534 tttgctggcatctttccaaaaaactatgaaccacctcctggtgatttttattttgaagtt 593
|||||||||||||| |||||||| ||||||||||| |||||||| ||||||||||||||
Sbjct: 1 tttgctggcatcttcccaaaaaattatgaaccaccacctggtgacttttattttgaagtc 60
Query: 594 gatgataattcccctgttgtccaggatgatccccttcttttcgattataatgttgaagaa 653
|||| || ||||||||||||||||||||||||||||||||| ||||| || |||||| ||
Sbjct: 61 gatgctacttcccctgttgtccaggatgatccccttctttttgattacaacgttgaacaa 120
Query: 654 cgagtaaatgattttgttgctgcagctgtagcacaggcaaatatcacgaggacaaatcat 713
|| || |||||||||||||||||||| | |||||||||||| | || |||||||| |||
Sbjct: 121 cgggtgaatgattttgttgctgcagcgctggcacaggcaaatgttacaaggacaaaccat 180
Query: 714 atcatgtttacaatgggaacagacttcaaataccaatacgcagagagttggttcggaaat 773
||||||||||||||||||||||||||||||| |||||||| || ||||||||| || |
Sbjct: 181 gtcatgtttacaatgggaacagacttcaaatatcaatacgcggaaagttggttcagacag 240
Query: 774 atggacaaactaattcactatgtgaacaaggatggccgtatccatgctctttattccaca 833
||||||||| ||||||| ||||||||||||||||||||| | ||||||| || || |||
Sbjct: 241 atggacaaattaattcattatgtgaacaaggatggccgtgtgaatgctctgtactctaca 300
Query: 834 ccttccatttataccgatgcaaaatatgctgcaaatgagc 873
||||||||||| ||||||||||||| | ||||||||||||
Sbjct: 301 ccttccatttacaccgatgcaaaattttctgcaaatgagc 340
>gb|BG416017.1|BG416017 HVSMEk0009H03f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0009H03f, mRNA sequence
Length = 955
Score = 365 bits (184), Expect = 2e-099
Identities = 358/416 (86%)
Strand = Plus / Plus
Query: 1 acgacgtcggatggctcaagaccgtagaccagtactacgtcggatccaacaactccatcc 60
|||||||||| |||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct: 107 acgacgtcggctggctcaagaccgtcgaccagtactacgtcggatccaacaactccatcc 166
Query: 61 agggggcatgcgtgcagaacgtgctggattcgctcatcccggcgcttctcaaggacgaga 120
|||| || ||||| ||||||||||||||||||||| | |||||||| ||||||||| |||
Sbjct: 167 agggcgcgtgcgttcagaacgtgctggattcgctcgtgccggcgctgctcaaggactaga 226
Query: 121 accgaaagttcatctatgttgagcaggcattcttccaaagatggtggaggaaccagaatg 180
|||| ||||||||||| || ||||||||||| ||||| || ||||||||| | || || |
Sbjct: 227 accgcaagttcatctacgtcgagcaggcatttttccacaggtggtggaggcaacacaacg 286
Query: 181 acatgatcaaggatacagtgaaggggctcatcagctctggacggctggaattaataaatg 240
|||| ||||||||||| |||||||||||| ||| ||| | ||||||||||| |||||||
Sbjct: 287 acattatcaaggatacggtgaaggggctcgtcaactcccggcggctggaattcataaatg 346
Query: 241 gtggcatgtgcatgcacgatgaggcggctgtacactatattgacatgatcgatcagacta 300
| || |||||||||||||| ||||| | || ||||| ||||||||||| ||||| ||||
Sbjct: 347 ggggtatgtgcatgcacgaccaggcgaccgtgcactacattgacatgattgatcacacta 406
Query: 301 cacttggacacaagtacatcaaggaggagtttggccagatcccacggattggctggcaga 360
| || || |||| || |||||||||||||||||| ||||| ||| |||||| ||||| |
Sbjct: 407 cgctgggccacaggttcatcaaggaggagtttggtcagattccaaagattggttggcaaa 466
Query: 361 ttgatccgtttgggcactctgcggttcaggcttaccttcttggcgcagaagtagga 416
| ||||||||||||||| ||| | || |||| |||||||| ||||||||||||
Sbjct: 467 taaatccgtttgggcactttgcaatacaagctttacttcttggagcagaagtagga 522
>gb|BI949337.1|BI949337 HVSMEl0013G14f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0013G14f, mRNA sequence
Length = 507
Score = 357 bits (180), Expect = 4e-097
Identities = 300/340 (88%)
Strand = Plus / Plus
Query: 534 tttgctggcatctttccaaaaaactatgaaccacctcctggtgatttttattttgaagtt 593
|||||||||||||| |||||||| ||||||||||| |||||||| ||||||||||||||
Sbjct: 1 tttgctggcatcttcccaaaaaattatgaaccaccacctggtgacttttattttgaagtc 60
Query: 594 gatgataattcccctgttgtccaggatgatccccttcttttcgattataatgttgaagaa 653
|||| | ||||||||||||||||||||||||||||||||| ||| | || |||||| ||
Sbjct: 61 gatgctgcttcccctgttgtccaggatgatccccttctttttgatcacaacgttgaacaa 120
Query: 654 cgagtaaatgattttgttgctgcagctgtagcacaggcaaatatcacgaggacaaatcat 713
|| || |||||||||||||||||||| | |||||||||||| | || |||||||| |||
Sbjct: 121 cgggtgaatgattttgttgctgcagcgctggcacaggcaaatgttacaaggacaaaccat 180
Query: 714 atcatgtttacaatgggaacagacttcaaataccaatacgcagagagttggttcggaaat 773
||||||||||||||||||||||||||||||| |||||||| || ||||||||| || |
Sbjct: 181 gtcatgtttacaatgggaacagacttcaaatatcaatacgcggaaagttggttcagacag 240
Query: 774 atggacaaactaattcactatgtgaacaaggatggccgtatccatgctctttattccaca 833
||||||||| ||||||| ||||||||||||||||||||| | ||||||| || || |||
Sbjct: 241 atggacaaattaattcattatgtgaacaaggatggccgtgtgaatgctctgtactctaca 300
Query: 834 ccttccatttataccgatgcaaaatatgctgcaaatgagc 873
||||||||||| ||||||||||||| | ||||||||||||
Sbjct: 301 ccttccatttacaccgatgcaaaattttctgcaaatgagc 340
>gb|BQ464262.1|BQ464262 HF01M14T HF Hordeum vulgare subsp. vulgare cDNA clone HF01M14
5-PRIME, mRNA sequence
Length = 591
Score = 232 bits (117), Expect = 2e-059
Identities = 201/229 (87%)
Strand = Plus / Plus
Query: 645 gttgaagaacgagtaaatgattttgttgctgcagctgtagcacaggcaaatatcacgagg 704
|||||| |||| || |||||||||||||||||||| | |||||||||||| | || |||
Sbjct: 9 gttgaacaacgggtgaatgattttgttgctgcagcgctggcacaggcaaatgttacaagg 68
Query: 705 acaaatcatatcatgtttacaatgggaacagacttcaaataccaatacgcagagagttgg 764
||||| ||| ||||||||||||||||||||||||||||||| |||||||| || ||||||
Sbjct: 69 acaaaccatgtcatgtttacaatgggaacagacttcaaatatcaatacgcggaaagttgg 128
Query: 765 ttcggaaatatggacaaactaattcactatgtgaacaaggatggccgtatccatgctctt 824
||| || | ||||||||| ||||||| ||||||||||||||||||||| | |||||||
Sbjct: 129 ttcagacagatggacaaattaattcattatgtgaacaaggatggccgtgtgaatgctctg 188
Query: 825 tattccacaccttccatttataccgatgcaaaatatgctgcaaatgagc 873
|| || |||||||||||||| ||||||||||||| | ||||||||||||
Sbjct: 189 tactctacaccttccatttacaccgatgcaaaattttctgcaaatgagc 237
>gb|BF255585.2|BF255585 HVSMEf0007E04f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0007E04f, mRNA sequence
Length = 874
Score = 206 bits (104), Expect = 9e-052
Identities = 179/204 (87%)
Strand = Plus / Plus
Query: 670 ttgctgcagctgtagcacaggcaaatatcacgaggacaaatcatatcatgtttacaatgg 729
|||||||||| | |||||||||||| | || |||||||| ||| |||||||||||||||
Sbjct: 1 ttgctgcagcgctggcacaggcaaatgttacaaggacaaaccatgtcatgtttacaatgg 60
Query: 730 gaacagacttcaaataccaatacgcagagagttggttcggaaatatggacaaactaattc 789
|||||||||||||||| |||||||| || ||||||||| || | ||||||||| ||||||
Sbjct: 61 gaacagacttcaaatatcaatacgcggaaagttggttcagacagatggacaaattaattc 120
Query: 790 actatgtgaacaaggatggccgtatccatgctctttattccacaccttccatttataccg 849
| ||||||||||||||||||||| | ||||||| || || |||||||||||||| ||||
Sbjct: 121 attatgtgaacaaggatggccgtgtgaatgctctgtactctacaccttccatttacaccg 180
Query: 850 atgcaaaatatgctgcaaatgagc 873
||||||||| | ||||||||||||
Sbjct: 181 atgcaaaattttctgcaaatgagc 204
>gb|BU974636.1|BU974636 HB28J16r BC Hordeum vulgare subsp. vulgare cDNA clone HB28J16
5-PRIME, mRNA sequence
Length = 370
Score = 50.1 bits (25), Expect = 1e-004
Identities = 46/53 (86%)
Strand = Plus / Plus
Query: 642 aatgttgaagaacgagtaaatgattttgttgctgcagctgtagcacaggcaaa 694
||||||||| |||| || |||||||||||| ||||||| ||| |||||||||
Sbjct: 143 aatgttgaacaacgcgttaatgattttgtttctgcagccatagtacaggcaaa 195
Score = 42.1 bits (21), Expect = 0.031
Identities = 81/101 (80%)
Strand = Plus / Plus
Query: 762 tggttcggaaatatggacaaactaattcactatgtgaacaaggatggccgtatccatgct 821
|||||| | |||||||| | ||| || || || |||||||||||||| || | |||||
Sbjct: 263 tggttccggaatatggatagacttatccattacgtgaacaaggatggacgagtgcatgca 322
Query: 822 ctttattccacaccttccatttataccgatgcaaaatatgc 862
|| ||||| || || |||||||| || ||||||||| ||||
Sbjct: 323 ctgtattctactccatccatttacactgatgcaaaacatgc 363
>gb|BQ471200.1|BQ471200 HV01J02T HV Hordeum vulgare subsp. vulgare cDNA clone HV01J02
5-PRIME, mRNA sequence
Length = 491
Score = 42.1 bits (21), Expect = 0.031
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 70 gcgtgcagaacgtgctggatt 90
|||||||||||||||||||||
Sbjct: 384 gcgtgcagaacgtgctggatt 404
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 92,169
Number of Sequences: 312970
Number of extensions: 92169
Number of successful extensions: 26047
Number of sequences better than 0.5: 10
Number of HSP's better than 0.5 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 26029
Number of HSP's gapped (non-prelim): 13
length of query: 888
length of database: 175,134,539
effective HSP length: 19
effective length of query: 869
effective length of database: 169,188,109
effective search space: 147024466721
effective search space used: 147024466721
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)