BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5532690.2.1
(863 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV923809.1|AV923809 AV923809 K. Sato unpublished cDNA li... 200 5e-050
gb|BM372365.2|BM372365 EBro03_SQ004_M03_R root, 3 week, wat... 200 5e-050
gb|BU966693.1|BU966693 HB02B04r BC Hordeum vulgare subsp. v... 200 5e-050
gb|CA020703.1|CA020703 HZ37D17r HZ Hordeum vulgare subsp. v... 200 5e-050
gb|BE194812.3|BE194812 HVSMEh0087A14f Hordeum vulgare 5-45 ... 125 3e-027
gb|AV918113.1|AV918113 AV918113 K. Sato unpublished cDNA li... 80 1e-013
gb|AV931883.1|AV931883 AV931883 K. Sato unpublished cDNA li... 80 1e-013
gb|AV927333.1|AV927333 AV927333 K. Sato unpublished cDNA li... 74 8e-012
gb|CA011092.1|CA011092 HT07B15u HT Hordeum vulgare subsp. v... 66 2e-009
gb|CA013000.1|CA013000 HT07B15r HT Hordeum vulgare subsp. v... 66 2e-009
gb|CD663740.1|CD663740 UCRHV18_06ca01_b1 Drought-stressed D... 66 2e-009
gb|CB881980.1|CB881980 HM11H21w HM Hordeum vulgare subsp. v... 60 1e-007
gb|BI960778.1|BI960778 HVSMEk0014L08f Hordeum vulgare testa... 40 0.12
>gb|AV923809.1|AV923809 AV923809 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd1j19 5', mRNA sequence
Length = 544
Score = 200 bits (101), Expect = 5e-050
Identities = 182/209 (87%)
Strand = Plus / Minus
Query: 625 tatccaaagccatacactacatctccagtgtaaaatcgatatgttctcccagggtagcct 684
|||||||| |||||||| || ||||| |||||||| ||||||||||| |||||||||||
Sbjct: 499 tatccaaaaccatacacaacttctccggtgtaaaaccgatatgttcttccagggtagccg 440
Query: 685 cgtgaaggatcagctctcatgttcatatcagtcatgggaatagcagtaaaggattcagga 744
|||||||| || |||||||||||||||||| |||| |||| || || |||||||| ||
Sbjct: 439 cgtgaagggtcggctctcatgttcatatcattcattggaaccgcggtgaaggattcgggg 380
Query: 745 taccaagttataggcagctttcctcctgggttgtactctccaaaaagaatttctggaagg 804
|||||||| || |||| ||||||||||| ||||||||||| |||||||||||||||||
Sbjct: 379 taccaagtcatgggcaattttcctcctggattgtactctccgaaaagaatttctggaaga 320
Query: 805 acttgtccaccaacctcgccaggatatcc 833
|||||||||||||| || || ||||||||
Sbjct: 319 acttgtccaccaacttcccctggatatcc 291
>gb|BM372365.2|BM372365 EBro03_SQ004_M03_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ004_M03 5', mRNA sequence
Length = 546
Score = 200 bits (101), Expect = 5e-050
Identities = 182/209 (87%)
Strand = Plus / Minus
Query: 625 tatccaaagccatacactacatctccagtgtaaaatcgatatgttctcccagggtagcct 684
|||||||| |||||||| || ||||| |||||||| ||||||||||| |||||||||||
Sbjct: 464 tatccaaaaccatacacaacttctccggtgtaaaaccgatatgttcttccagggtagccg 405
Query: 685 cgtgaaggatcagctctcatgttcatatcagtcatgggaatagcagtaaaggattcagga 744
|||||||| || |||||||||||||||||| |||| |||| || || |||||||| ||
Sbjct: 404 cgtgaagggtcggctctcatgttcatatcattcattggaaccgcggtgaaggattcgggg 345
Query: 745 taccaagttataggcagctttcctcctgggttgtactctccaaaaagaatttctggaagg 804
|||||||| || |||| ||||||||||| ||||||||||| |||||||||||||||||
Sbjct: 344 taccaagtcatgggcaattttcctcctggattgtactctccgaaaagaatttctggaaga 285
Query: 805 acttgtccaccaacctcgccaggatatcc 833
|||||||||||||| || || ||||||||
Sbjct: 284 acttgtccaccaacttcccctggatatcc 256
>gb|BU966693.1|BU966693 HB02B04r BC Hordeum vulgare subsp. vulgare cDNA clone HB02B04
5-PRIME, mRNA sequence
Length = 552
Score = 200 bits (101), Expect = 5e-050
Identities = 182/209 (87%)
Strand = Plus / Minus
Query: 625 tatccaaagccatacactacatctccagtgtaaaatcgatatgttctcccagggtagcct 684
|||||||| |||||||| || ||||| |||||||| ||||||||||| |||||||||||
Sbjct: 328 tatccaaaaccatacacaacttctccggtgtaaaaccgatatgttcttccagggtagccg 269
Query: 685 cgtgaaggatcagctctcatgttcatatcagtcatgggaatagcagtaaaggattcagga 744
|||||||| || |||||||||||||||||| |||| |||| || || |||||||| ||
Sbjct: 268 cgtgaagggtcggctctcatgttcatatcattcattggaaccgcggtgaaggattcgggg 209
Query: 745 taccaagttataggcagctttcctcctgggttgtactctccaaaaagaatttctggaagg 804
|||||||| || |||| ||||||||||| ||||||||||| |||||||||||||||||
Sbjct: 208 taccaagtcatgggcaattttcctcctggattgtactctccgaaaagaatttctggaaga 149
Query: 805 acttgtccaccaacctcgccaggatatcc 833
|||||||||||||| || || ||||||||
Sbjct: 148 acttgtccaccaacttcccctggatatcc 120
Score = 48.1 bits (24), Expect = 5e-004
Identities = 75/92 (81%)
Strand = Plus / Minus
Query: 460 acagagaataccagagcttcacatgacgcgatatcttcagttttcacaaagtctagtcca 519
||||| |||||||| | |||||||| ||||| |||||| || ||| |||| | |||
Sbjct: 505 acagaaaataccagggactcacatgatgcgatgtcttcaacttggacacagtccaatccg 446
Query: 520 tccctccttgtgtatgcaggctttctgctgat 551
||||||| ||||||||||||||| || |||||
Sbjct: 445 tccctccgtgtgtatgcaggcttcctactgat 414
>gb|CA020703.1|CA020703 HZ37D17r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ37D17
5-PRIME, mRNA sequence
Length = 624
Score = 200 bits (101), Expect = 5e-050
Identities = 182/209 (87%)
Strand = Plus / Minus
Query: 625 tatccaaagccatacactacatctccagtgtaaaatcgatatgttctcccagggtagcct 684
|||||||| |||||||| || ||||| |||||||| ||||||||||| |||||||||||
Sbjct: 419 tatccaaaaccatacacaacttctccggtgtaaaaccgatatgttcttccagggtagccg 360
Query: 685 cgtgaaggatcagctctcatgttcatatcagtcatgggaatagcagtaaaggattcagga 744
|||||||| || |||||||||||||||||| |||| |||| || || |||||||| ||
Sbjct: 359 cgtgaagggtcggctctcatgttcatatcattcattggaaccgcggtgaaggattcgggg 300
Query: 745 taccaagttataggcagctttcctcctgggttgtactctccaaaaagaatttctggaagg 804
|||||||| || |||| ||||||||||| ||||||||||| |||||||||||||||||
Sbjct: 299 taccaagtcatgggcaattttcctcctggattgtactctccgaaaagaatttctggaaga 240
Query: 805 acttgtccaccaacctcgccaggatatcc 833
|||||||||||||| || || ||||||||
Sbjct: 239 acttgtccaccaacttcccctggatatcc 211
Score = 48.1 bits (24), Expect = 5e-004
Identities = 75/92 (81%)
Strand = Plus / Minus
Query: 460 acagagaataccagagcttcacatgacgcgatatcttcagttttcacaaagtctagtcca 519
||||| |||||||| | |||||||| ||||| |||||| || ||| |||| | |||
Sbjct: 596 acagaaaataccagggactcacatgatgcgatgtcttcaacttggacacagtccaatccg 537
Query: 520 tccctccttgtgtatgcaggctttctgctgat 551
||||||| ||||||||||||||| || |||||
Sbjct: 536 tccctccgtgtgtatgcaggcttcctactgat 505
>gb|BE194812.3|BE194812 HVSMEh0087A14f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0087A14f, mRNA sequence
Length = 652
Score = 125 bits (63), Expect = 3e-027
Identities = 175/209 (83%), Gaps = 3/209 (1%)
Strand = Plus / Minus
Query: 625 tatccaaagccatacactacatctccagtgtaaaatcgatatgttctcccagggtagcct 684
|||||||| |||||||| | ||||| ||||||| ||||||||||| | ||| |||||
Sbjct: 520 tatccaaacccatacacaccttctccggtgtaaac-cgatatgttcttcaagg-tagccg 463
Query: 685 cgtgaaggatcagctctcatgttcatatcagtcatgggaatagcagtaaaggattcagga 744
|||| | | || |||||||||||||||||| |||| |||| || || |||||||| ||
Sbjct: 462 cgtgnaaggtcggctctcatgttcatatcattcattggaaccgcggtgaaggattcgggg 403
Query: 745 taccaagttataggcagctttcctcctgggttgtactctccaaaaagaatttctggaagg 804
|||||||| || |||| ||||||| ||| ||||||||||| |||||||||||||||||
Sbjct: 402 taccaagtcatgggcat-tttcctcntggattgtactctccgaaaagaatttctggaaga 344
Query: 805 acttgtccaccaacctcgccaggatatcc 833
|||||||||||||| || || ||||||||
Sbjct: 343 acttgtccaccaacttcccctggatatcc 315
Score = 38.2 bits (19), Expect = 0.47
Identities = 22/23 (95%)
Strand = Plus / Minus
Query: 520 tccctccttgtgtatgcaggctt 542
||||||| |||||||||||||||
Sbjct: 637 tccctccgtgtgtatgcaggctt 615
>gb|AV918113.1|AV918113 AV918113 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags17o10 3', mRNA sequence
Length = 597
Score = 79.8 bits (40), Expect = 1e-013
Identities = 223/284 (78%)
Strand = Plus / Plus
Query: 268 ttggctgcgctcatatgcttgcaggggtcaacagtaatctccacactggatgcactgcca 327
||||| |||||||||| |||||||| || || || ||| ||||| | || ||||||
Sbjct: 232 ttggccgcgctcatatatttgcagggatccaccgtgatcgccacattttttgaactgccg 291
Query: 328 gccgccgtgtggacacgctcaaagccaaccagctgttttataggaaagcccgggacgctt 387
|| || |||| ||| |||||||| |||||||||||||| | ||| |||||||| |||||
Sbjct: 292 gcggcagtgtagacgcgctcaaaaccaaccagctgtttcagagggaagcccggaacgctc 351
Query: 388 gatttcgatctcgcgaacagaagaacagcatgactcccatccatgctaccatgattcgaa 447
|| |||||||| |||||||| | || ||||| ||||| |||||| ||| | || |
Sbjct: 352 gacttcgatcttgcgaacagtaagacggcatggctcccgtccatggcaccgtcgttggcg 411
Query: 448 acagcgacgtgaacagagaataccagagcttcacatgacgcgatatcttcagttttcaca 507
|| | || ||| ||||| |||||||| | |||||||| ||||| |||||| || |||
Sbjct: 412 accgagatgtggacagaaaataccagggactcacatgatgcgatgtcttcaacttggaca 471
Query: 508 aagtctagtccatccctccttgtgtatgcaggctttctgctgat 551
|||| | ||| ||||||| ||||||||||||||| || |||||
Sbjct: 472 tagtccaatccgtccctccgtgtgtatgcaggcttcctactgat 515
>gb|AV931883.1|AV931883 AV931883 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd26i20 3', mRNA sequence
Length = 598
Score = 79.8 bits (40), Expect = 1e-013
Identities = 223/284 (78%)
Strand = Plus / Plus
Query: 268 ttggctgcgctcatatgcttgcaggggtcaacagtaatctccacactggatgcactgcca 327
||||| |||||||||| |||||||| || || || ||| ||||| | || ||||||
Sbjct: 233 ttggccgcgctcatatatttgcagggatccaccgtgatcgccacattttttgaactgccg 292
Query: 328 gccgccgtgtggacacgctcaaagccaaccagctgttttataggaaagcccgggacgctt 387
|| || |||| ||| |||||||| |||||||||||||| | ||| |||||||| |||||
Sbjct: 293 gcggcagtgtagacgcgctcaaaaccaaccagctgtttcagagggaagcccggaacgctc 352
Query: 388 gatttcgatctcgcgaacagaagaacagcatgactcccatccatgctaccatgattcgaa 447
|| |||||||| |||||||| | || ||||| ||||| |||||| ||| | || |
Sbjct: 353 gacttcgatcttgcgaacagtaagacggcatggctcccgtccatggcaccgtcgttggcg 412
Query: 448 acagcgacgtgaacagagaataccagagcttcacatgacgcgatatcttcagttttcaca 507
|| | || ||| ||||| |||||||| | |||||||| ||||| |||||| || |||
Sbjct: 413 accgagatgtggacagaaaataccagggactcacatgatgcgatgtcttcaacttggaca 472
Query: 508 aagtctagtccatccctccttgtgtatgcaggctttctgctgat 551
|||| | ||| ||||||| ||||||||||||||| || |||||
Sbjct: 473 tagtccaatccgtccctccgtgtgtatgcaggcttcctactgat 516
>gb|AV927333.1|AV927333 AV927333 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd1j19 3', mRNA sequence
Length = 657
Score = 73.8 bits (37), Expect = 8e-012
Identities = 222/284 (78%)
Strand = Plus / Plus
Query: 268 ttggctgcgctcatatgcttgcaggggtcaacagtaatctccacactggatgcactgcca 327
||||| |||||||||| |||||||| || || || ||| ||||| | || ||||||
Sbjct: 240 ttggccgcgctcatatatttgcagggatccaccgtgatcgccacattttttgaactgccg 299
Query: 328 gccgccgtgtggacacgctcaaagccaaccagctgttttataggaaagcccgggacgctt 387
|| || |||| ||| |||||||| |||||||||||||| | ||| |||||||| |||||
Sbjct: 300 gcggcagtgtagacgcgctcaaaaccaaccagctgtttcagagggaagcccggaacgctc 359
Query: 388 gatttcgatctcgcgaacagaagaacagcatgactcccatccatgctaccatgattcgaa 447
|| |||||||| |||||||| | || ||||| ||||| |||||| ||| || |
Sbjct: 360 gacttcgatcttgcgaacagtaagacggcatggctcccgtccatggcaccgncgttggcg 419
Query: 448 acagcgacgtgaacagagaataccagagcttcacatgacgcgatatcttcagttttcaca 507
|| | || ||| ||||| |||||||| | |||||||| ||||| |||||| || |||
Sbjct: 420 accgagatgtggacagaaaataccagggactcacatgatgcgatgtcttcaacttggaca 479
Query: 508 aagtctagtccatccctccttgtgtatgcaggctttctgctgat 551
|||| | ||| ||||||| ||||||||||||||| || |||||
Sbjct: 480 tagtccaatccgtccctccgtgtgtatgcaggcttcctactgat 523
Score = 42.1 bits (21), Expect = 0.030
Identities = 40/47 (85%)
Strand = Plus / Plus
Query: 625 tatccaaagccatacactacatctccagtgtaaaatcgatatgttct 671
||| |||| |||||||| || ||| | |||||||| |||||||||||
Sbjct: 609 tatncaaaaccatacacaacttctncggtgtaaaaccgatatgttct 655
>gb|CA011092.1|CA011092 HT07B15u HT Hordeum vulgare subsp. vulgare cDNA clone HT07B15
3-PRIME, mRNA sequence
Length = 503
Score = 65.9 bits (33), Expect = 2e-009
Identities = 132/165 (80%)
Strand = Plus / Plus
Query: 268 ttggctgcgctcatatgcttgcaggggtcaacagtaatctccacactggatgcactgcca 327
||||| |||||||||| |||||||| || || || ||| ||||| | || ||||||
Sbjct: 244 ttggccgcgctcatatatttgcagggatccaccgtgatcgccacattttttgaactgccg 303
Query: 328 gccgccgtgtggacacgctcaaagccaaccagctgttttataggaaagcccgggacgctt 387
|| || |||| ||| ||||| || |||||||||||||| | ||| |||||||| |||||
Sbjct: 304 gcggcagtgtagacgcgctcgaaaccaaccagctgtttcagagggaagcccggaacgctc 363
Query: 388 gatttcgatctcgcgaacagaagaacagcatgactcccatccatg 432
|| |||||||| |||||||| | || ||||| ||||| ||||||
Sbjct: 364 gacttcgatcttgcgaacagtaagacggcatggctcccgtccatg 408
>gb|CA013000.1|CA013000 HT07B15r HT Hordeum vulgare subsp. vulgare cDNA clone HT07B15
5-PRIME, mRNA sequence
Length = 501
Score = 65.9 bits (33), Expect = 2e-009
Identities = 132/165 (80%)
Strand = Plus / Minus
Query: 268 ttggctgcgctcatatgcttgcaggggtcaacagtaatctccacactggatgcactgcca 327
||||| |||||||||| |||||||| || || || ||| ||||| | || ||||||
Sbjct: 258 ttggccgcgctcatatatttgcagggatccaccgtgatcgccacattttttgaactgccg 199
Query: 328 gccgccgtgtggacacgctcaaagccaaccagctgttttataggaaagcccgggacgctt 387
|| || |||| ||| ||||| || |||||||||||||| | ||| |||||||| |||||
Sbjct: 198 gcggcagtgtagacgcgctcgaaaccaaccagctgtttcagagggaagcccggaacgctc 139
Query: 388 gatttcgatctcgcgaacagaagaacagcatgactcccatccatg 432
|| |||||||| |||||||| | || ||||| ||||| ||||||
Sbjct: 138 gacttcgatcttgcgaacagtaagacggcatggctcccgtccatg 94
>gb|CD663740.1|CD663740 UCRHV18_06ca01_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_06ca01, mRNA sequence
Length = 489
Score = 65.9 bits (33), Expect = 2e-009
Identities = 132/165 (80%)
Strand = Plus / Plus
Query: 268 ttggctgcgctcatatgcttgcaggggtcaacagtaatctccacactggatgcactgcca 327
||||| |||||||||| |||||||| || || || ||| ||||| | || ||||||
Sbjct: 258 ttggccgcgctcatatatttgcagggatccaccgtgatcgccacattttttgaactgccg 317
Query: 328 gccgccgtgtggacacgctcaaagccaaccagctgttttataggaaagcccgggacgctt 387
|| || |||| ||| ||||| || |||||||||||||| | ||| |||||||| |||||
Sbjct: 318 gcggcagtgtagacgcgctcgaaaccaaccagctgtttcagagggaagcccggaacgctc 377
Query: 388 gatttcgatctcgcgaacagaagaacagcatgactcccatccatg 432
|| |||||||| |||||||| | || ||||| ||||| ||||||
Sbjct: 378 gacttcgatcttgcgaacagtaagacggcatggctcccgtccatg 422
>gb|CB881980.1|CB881980 HM11H21w HM Hordeum vulgare subsp. vulgare cDNA clone HM11H21
3-PRIME, mRNA sequence
Length = 518
Score = 60.0 bits (30), Expect = 1e-007
Identities = 75/90 (83%)
Strand = Plus / Plus
Query: 343 cgctcaaagccaaccagctgttttataggaaagcccgggacgcttgatttcgatctcgcg 402
||||| || |||||||||||||| | ||| |||||||| ||||| || |||||||| |||
Sbjct: 325 cgctcgaaaccaaccagctgtttcagagggaagcccggaacgctcgacttcgatcttgcg 384
Query: 403 aacagaagaacagcatgactcccatccatg 432
||||| | || ||||| ||||| ||||||
Sbjct: 385 aacagtaagacggcatggctcccgtccatg 414
>gb|BI960778.1|BI960778 HVSMEk0014L08f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0014L08f, mRNA sequence
Length = 689
Score = 40.1 bits (20), Expect = 0.12
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 87 agctcaatgatgaatcttta 106
||||||||||||||||||||
Sbjct: 506 agctcaatgatgaatcttta 525
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 67,440
Number of Sequences: 312970
Number of extensions: 67440
Number of successful extensions: 16587
Number of sequences better than 0.5: 13
Number of HSP's better than 0.5 without gapping: 13
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 16560
Number of HSP's gapped (non-prelim): 26
length of query: 863
length of database: 175,134,539
effective HSP length: 19
effective length of query: 844
effective length of database: 169,188,109
effective search space: 142794763996
effective search space used: 142794763996
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)