BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5110719.2.1
(581 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV916139.1|AV916139 AV916139 K. Sato unpublished cDNA li... 285 8e-076
gb|BQ765720.1|BQ765720 EBro03_SQ008_A02_R root, 3 week, wat... 246 7e-064
gb|BU998550.1|BU998550 HI11G16r HI Hordeum vulgare subsp. v... 246 7e-064
gb|AL506334.1|AL506334 AL506334 Hordeum vulgare Barke devel... 214 2e-054
gb|BF627268.3|BF627268 HVSMEb0004G12f Hordeum vulgare seedl... 153 8e-036
gb|BJ470706.1|BJ470706 BJ470706 K. Sato unpublished cDNA li... 127 4e-028
gb|BJ473004.1|BJ473004 BJ473004 K. Sato unpublished cDNA li... 125 2e-027
gb|AL503993.1|AL503993 AL503993 Hordeum vulgare Barke roots... 86 1e-015
gb|AV914721.1|AV914721 AV914721 K. Sato unpublished cDNA li... 80 9e-014
gb|BM816998.1|BM816998 HB107H12_SK.ab1 HB Hordeum vulgare s... 68 3e-010
gb|BJ477170.1|BJ477170 BJ477170 K. Sato unpublished cDNA li... 66 1e-009
gb|BQ469442.1|BQ469442 HM04L11r HM Hordeum vulgare subsp. v... 64 5e-009
gb|CB882775.1|CB882775 HL02N01w HL Hordeum vulgare subsp. v... 38 0.31
>gb|AV916139.1|AV916139 AV916139 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags1l14 5', mRNA sequence
Length = 642
Score = 285 bits (144), Expect = 8e-076
Identities = 324/384 (84%)
Strand = Plus / Plus
Query: 2 attaacaatataccatgtaaaaagtcacttgcagaaatacagactagcaaagtatattcc 61
|||||||||||||||||| ||||||||||||||||||||||| ||||||||||| |||||
Sbjct: 74 attaacaatataccatgtgaaaagtcacttgcagaaatacaggctagcaaagtacattcc 133
Query: 62 tgatgcttcaactgatgggaacaagaccgataacaaagacccaggagatttgcttgccgg 121
|| ||||| ||||||| |||||| | ||||||||||| ||||| |||| | || ||
Sbjct: 134 cgactcttcagctgatggtaacaaggctgataacaaagatccaggggattcattagcagg 193
Query: 122 acttgagggatcctctggattgcagatatctgaagccctcaaactgcagatggaggtcca 181
|||||| |||||||||||| ||||||||||||||||||| || |||||||||||||||||
Sbjct: 194 acttgatggatcctctggaatgcagatatctgaagccctgaagctgcagatggaggtcca 253
Query: 182 aaagcggctgcacgaacagttagaagtacaaaggcagctgcagttacgaattgaggctca 241
||||||| |||| |||||| | ||||||||||||||||||||| | ||||| ||||| ||
Sbjct: 254 aaagcggttgcatgaacagctcgaagtacaaaggcagctgcagctgcgaatcgaggcaca 313
Query: 242 gggaaagtacctccagaagatcatcgaagagcagcagcgcttaacaggtgtgaaatctga 301
|||||||||||| |||| ||||| |||||||| ||||| || ||| | || |||||
Sbjct: 314 gggaaagtaccttaagaaaatcatagaagagcaacagcgtttcggtggtataaagtctga 373
Query: 302 aactcctgctggaggtgcttctgtcactgtatcaagtgatcaattcccagactcggagcg 361
||| |||| || ||| ||| ||||||| | || || ||||| ||||| ||||| ||| |
Sbjct: 374 aacacctggtgcaggagctactgtcacagcgtcgagcgatcagttccctgactctgagag 433
Query: 362 gactgagccctccacacctgcacc 385
||| || ||||| || ||||||||
Sbjct: 434 gacagatccctcgactcctgcacc 457
>gb|BQ765720.1|BQ765720 EBro03_SQ008_A02_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_A02 5', mRNA sequence
Length = 626
Score = 246 bits (124), Expect = 7e-064
Identities = 199/224 (88%)
Strand = Plus / Plus
Query: 1 gattaacaatataccatgtaaaaagtcacttgcagaaatacagactagcaaagtatattc 60
||||||||||||||||||| ||||||||||||||||||||||| ||||||||||| ||||
Sbjct: 392 gattaacaatataccatgtgaaaagtcacttgcagaaatacaggctagcaaagtacattc 451
Query: 61 ctgatgcttcaactgatgggaacaagaccgataacaaagacccaggagatttgcttgccg 120
| || ||||| ||||||| |||||| | ||||||||||| ||||| |||| | || |
Sbjct: 452 ccgactcttcagctgatggtaacaaggctgataacaaagatccaggggattcattagcag 511
Query: 121 gacttgagggatcctctggattgcagatatctgaagccctcaaactgcagatggaggtcc 180
||||||| |||||||||||| ||||||||||||||||||| || ||||||||||||||||
Sbjct: 512 gacttgatggatcctctggaatgcagatatctgaagccctgaagctgcagatggaggtcc 571
Query: 181 aaaagcggctgcacgaacagttagaagtacaaaggcagctgcag 224
|||||||| |||| |||||| | |||||||||||||||||||||
Sbjct: 572 aaaagcggttgcatgaacagctcgaagtacaaaggcagctgcag 615
>gb|BU998550.1|BU998550 HI11G16r HI Hordeum vulgare subsp. vulgare cDNA clone HI11G16
5-PRIME, mRNA sequence
Length = 625
Score = 246 bits (124), Expect = 7e-064
Identities = 199/224 (88%)
Strand = Plus / Plus
Query: 1 gattaacaatataccatgtaaaaagtcacttgcagaaatacagactagcaaagtatattc 60
||||||||||||||||||| ||||||||||||||||||||||| ||||||||||| ||||
Sbjct: 392 gattaacaatataccatgtgaaaagtcacttgcagaaatacaggctagcaaagtacattc 451
Query: 61 ctgatgcttcaactgatgggaacaagaccgataacaaagacccaggagatttgcttgccg 120
| || ||||| ||||||| |||||| | ||||||||||| ||||| |||| | || |
Sbjct: 452 ccgactcttcagctgatggtaacaaggctgataacaaagatccaggggattcattagcag 511
Query: 121 gacttgagggatcctctggattgcagatatctgaagccctcaaactgcagatggaggtcc 180
||||||| |||||||||||| ||||||||||||||||||| || ||||||||||||||||
Sbjct: 512 gacttgatggatcctctggaatgcagatatctgaagccctgaagctgcagatggaggtcc 571
Query: 181 aaaagcggctgcacgaacagttagaagtacaaaggcagctgcag 224
|||||||| |||| |||||| | |||||||||||||||||||||
Sbjct: 572 aaaagcggttgcatgaacagctcgaagtacaaaggcagctgcag 615
>gb|AL506334.1|AL506334 AL506334 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY02M13T 5',
mRNA sequence
Length = 700
Score = 214 bits (108), Expect = 2e-054
Identities = 177/200 (88%)
Strand = Plus / Plus
Query: 1 gattaacaatataccatgtaaaaagtcacttgcagaaatacagactagcaaagtatattc 60
||||||||||||||||||| ||||||||||||||||||||||| ||||||||||| ||||
Sbjct: 472 gattaacaatataccatgtgaaaagtcacttgcagaaatacaggctagcaaagtacattc 531
Query: 61 ctgatgcttcaactgatgggaacaagaccgataacaaagacccaggagatttgcttgccg 120
| || ||||| ||||||| |||||| | ||||||||||| ||||| |||| | || |
Sbjct: 532 ccgactcttcagctgatggtaacaaggctgataacaaagatccaggggattcattagcag 591
Query: 121 gacttgagggatcctctggattgcagatatctgaagccctcaaactgcagatggaggtcc 180
||||||| |||||||||||| ||||||||||||||||||| || ||||||||||||||||
Sbjct: 592 gacttgatggatcctctggaatgcagatatctgaagccctgaagctgcagatggaggtcc 651
Query: 181 aaaagcggctgcacgaacag 200
|||||||| |||| ||||||
Sbjct: 652 aaaagcggttgcatgaacag 671
>gb|BF627268.3|BF627268 HVSMEb0004G12f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0004G12f, mRNA sequence
Length = 875
Score = 153 bits (77), Expect = 8e-036
Identities = 192/226 (84%), Gaps = 4/226 (1%)
Strand = Plus / Plus
Query: 1 gattaacaatataccatgtaaaaagtcacttgcagaaatacagactagcaaagtatattc 60
||||||||||||||||||| ||||||||||||||||||||||| |||||| |||| ||||
Sbjct: 416 gattaacaatataccatgtgaaaagtcacttgcagaaatacaggctagcanagtacattc 475
Query: 61 ctgatgcttcaactgatgggaacaagaccgataacaaagacccaggagatttgcttgccg 120
| || ||||| ||||||| |||||| | ||| ||| ||| ||||| |||| | || |
Sbjct: 476 ccgactcttcagctgatggtaacaaggctgatnacanagatccaggggattcattagcag 535
Query: 121 gacttgagggat-cctctggattgcagatatc-tgaagccctcaaactgcagat-ggagg 177
||||||| |||| |||||||| |||||||||| ||| |||| || |||||||| |||||
Sbjct: 536 gacttgatggatccctctgga-tgcagatatcttgagcccctgaagctgcagatgggagg 594
Query: 178 tccaaaagcggctgcacgaacagttagaagtacaaaggcagctgca 223
||||||||||| |||| |||||| | |||||||||| |||||||||
Sbjct: 595 tccaaaagcggttgcatgaacagctcgaagtacaaaagcagctgca 640
>gb|BJ470706.1|BJ470706 BJ470706 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal26b02 5', mRNA sequence
Length = 537
Score = 127 bits (64), Expect = 4e-028
Identities = 118/136 (86%)
Strand = Plus / Plus
Query: 1 gattaacaatataccatgtaaaaagtcacttgcagaaatacagactagcaaagtatattc 60
||||||||||||||||||| ||||||||||||||||||||||| ||||||||||| ||||
Sbjct: 380 gattaacaatataccatgtgaaaagtcacttgcagaaatacaggctagcaaagtacattc 439
Query: 61 ctgatgcttcaactgatgggaacaagaccgataacaaagacccaggagatttgcttgccg 120
| || ||||| ||||||| |||||| | ||||||||||| ||||| |||| | || |
Sbjct: 440 ccgactcttcagctgatggtaacaaggctgataacaaagatccaggggattcattagcag 499
Query: 121 gacttgagggatcctc 136
||||||| ||||||||
Sbjct: 500 gacttgatggatcctc 515
>gb|BJ473004.1|BJ473004 BJ473004 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal40f15 5', mRNA sequence
Length = 499
Score = 125 bits (63), Expect = 2e-027
Identities = 99/111 (89%)
Strand = Plus / Plus
Query: 1 gattaacaatataccatgtaaaaagtcacttgcagaaatacagactagcaaagtatattc 60
||||||||||||||||||| ||||||||||||||||||||||| ||||||||||| ||||
Sbjct: 367 gattaacaatataccatgtgaaaagtcacttgcagaaatacaggctagcaaagtacattc 426
Query: 61 ctgatgcttcaactgatgggaacaagaccgataacaaagacccaggagatt 111
| || ||||| ||||||| |||||| | ||||||||||| ||||| ||||
Sbjct: 427 ccgactcttcagctgatggtaacaaggctgataacaaagatccaggggatt 477
>gb|AL503993.1|AL503993 AL503993 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW01E19V 5', mRNA sequence
Length = 622
Score = 85.7 bits (43), Expect = 1e-015
Identities = 99/114 (86%), Gaps = 3/114 (2%)
Strand = Plus / Plus
Query: 1 gattaacaatataccatgtaaaaagtcacttgca-gaaatacagact-agcaaagtatat 58
||||||||||||||||||| |||||||||||||| ||||||||| || ||||||||| ||
Sbjct: 432 gattaacaatataccatgtgaaaagtcacttgcangaaatacaggctaagcaaagtacat 491
Query: 59 tcctgatgcttcaactgatggg-aacaagaccgataacaaagacccaggagatt 111
||| || ||||| |||||||| |||||| | ||||||| ||| ||||| ||||
Sbjct: 492 tcccgactcttcagctgatgggtaacaaggctgataacanagatccaggggatt 545
>gb|AV914721.1|AV914721 AV914721 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags7m02 5', mRNA sequence
Length = 524
Score = 79.8 bits (40), Expect = 9e-014
Identities = 145/180 (80%)
Strand = Plus / Plus
Query: 206 agtacaaaggcagctgcagttacgaattgaggctcagggaaagtacctccagaagatcat 265
||||||||||||||||||| | ||||| ||||| |||||||||||||| |||| |||||
Sbjct: 12 agtacaaaggcagctgcagctgcgaatcgaggcacagggaaagtaccttaagaaaatcat 71
Query: 266 cgaagagcagcagcgcttaacaggtgtgaaatctgaaactcctgctggaggtgcttctgt 325
|||||||| ||||| || ||| | || |||||||| |||| || ||| ||| ||||
Sbjct: 72 agaagagcaacagcgtttcggtggtataaagtctgaaacacctggtgcaggagctactgt 131
Query: 326 cactgtatcaagtgatcaattcccagactcggagcggactgagccctccacacctgcacc 385
||| | || || ||||| ||||| ||||| ||| |||| || ||||| || ||||||||
Sbjct: 132 cacagcgtcgagcgatcagttccctgactctgagaggacagatccctcgactcctgcacc 191
>gb|BM816998.1|BM816998 HB107H12_SK.ab1 HB Hordeum vulgare subsp. vulgare cDNA clone
HB107H12_SK.ab1 similar to (AC006837) transfactor-like
protein [Arabidopsis thaliana], mRNA sequence
Length = 754
Score = 67.9 bits (34), Expect = 3e-010
Identities = 146/182 (80%), Gaps = 1/182 (0%)
Strand = Plus / Plus
Query: 204 gaagtacaaaggcagctgcagttacgaattgaggctcagggaaagtacctccagaagatc 263
||||||||||| ||||||||| | ||||| ||||| |||||||||||||| |||| |||
Sbjct: 29 gaagtacaaag-cagctgcagctgcgaatcgaggcacagggaaagtaccttaagaaaatc 87
Query: 264 atcgaagagcagcagcgcttaacaggtgtgaaatctgaaactcctgctggaggtgcttct 323
|| |||||||| ||||| || ||| | || |||||||| |||| || ||| ||| ||
Sbjct: 88 atagaagagcaacagcgtttcggtggtataaagtctgaaacacctggtgcaggagctact 147
Query: 324 gtcactgtatcaagtgatcaattcccagactcggagcggactgagccctccacacctgca 383
||||| | || || ||||| ||||| ||||| ||| |||| || ||||| || ||||||
Sbjct: 148 gtcacagcgtcgagcgatcagttccctgactctgagaggacagatccctcgactcctgca 207
Query: 384 cc 385
||
Sbjct: 208 cc 209
>gb|BJ477170.1|BJ477170 BJ477170 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal40f15 3', mRNA sequence
Length = 661
Score = 65.9 bits (33), Expect = 1e-009
Identities = 138/173 (79%)
Strand = Plus / Minus
Query: 213 aggcagctgcagttacgaattgaggctcagggaaagtacctccagaagatcatcgaagag 272
|||||||||||| | ||||| ||||| |||||||||||||| |||| ||||| ||||||
Sbjct: 661 aggcagctgcagctgcgaatcgaggcacagggaaagtaccttaagaaaatcatagaagag 602
Query: 273 cagcagcgcttaacaggtgtgaaatctgaaactcctgctggaggtgcttctgtcactgta 332
|| ||||| || ||| | || |||||||| |||| || ||| ||| ||||||| |
Sbjct: 601 caacagcgtttcggtggtataaagtctgaaacacctggtgcaggagctactgtcacagcg 542
Query: 333 tcaagtgatcaattcccagactcggagcggactgagccctccacacctgcacc 385
|| || ||||| ||||| ||||| ||| |||| || ||||| || ||||||||
Sbjct: 541 tcgagcgatcagttccctgactctgagaggacagatccctcgactcctgcacc 489
>gb|BQ469442.1|BQ469442 HM04L11r HM Hordeum vulgare subsp. vulgare cDNA clone HM04L11
5-PRIME, mRNA sequence
Length = 651
Score = 63.9 bits (32), Expect = 5e-009
Identities = 95/116 (81%)
Strand = Plus / Plus
Query: 166 tgcagatggaggtccaaaagcggctgcacgaacagttagaagtacaaaggcagctgcagt 225
||||| ||||||| || ||||| || | ||||| ||||| |||||||||||||| |||
Sbjct: 161 tgcaggtggaggtgcagaagcgtctacgtgaacaattagaggtacaaaggcagctacagc 220
Query: 226 tacgaattgaggctcagggaaagtacctccagaagatcatcgaagagcagcagcgc 281
| |||| || || || || ||||| |||||||||||||| || ||||||||||||
Sbjct: 221 tcagaatagaagcccaaggcaagtatctccagaagatcatggaggagcagcagcgc 276
>gb|CB882775.1|CB882775 HL02N01w HL Hordeum vulgare subsp. vulgare cDNA clone HL02N01
3-PRIME, mRNA sequence
Length = 617
Score = 38.2 bits (19), Expect = 0.31
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 59 tcctgatgcttcaactgat 77
|||||||||||||||||||
Sbjct: 158 tcctgatgcttcaactgat 176
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 61,112
Number of Sequences: 312970
Number of extensions: 61112
Number of successful extensions: 13283
Number of sequences better than 0.5: 13
Number of HSP's better than 0.5 without gapping: 13
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 13259
Number of HSP's gapped (non-prelim): 18
length of query: 581
length of database: 175,134,539
effective HSP length: 19
effective length of query: 562
effective length of database: 169,188,109
effective search space: 95083717258
effective search space used: 95083717258
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)