BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3871943.2.1
(1085 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA,... 155 4e-036
gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike... 129 2e-028
gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthe... 70 2e-010
gb|CK122193.1|CK122193 BES1824101p15 BES1824 Hordeum vulgar... 62 4e-008
gb|CK122985.1|CK122985 BES1824101k13 BES1824 Hordeum vulgar... 62 4e-008
gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, wat... 58 6e-007
gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA li... 52 4e-005
gb|BQ661505.1|BQ661505 HM03L14u HM Hordeum vulgare subsp. v... 50 2e-004
gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. v... 50 2e-004
gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. v... 50 2e-004
gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Li... 42 0.038
gb|AL450714.1|AL450714 AL450714 Hordeum vulgare Barke etiol... 42 0.038
gb|BI951306.1|BI951306 HVSMEl0025H05f Hordeum vulgare spike... 42 0.038
gb|BE216143.1|BE216143 HV_CEb0009J07f Hordeum vulgare seedl... 42 0.038
gb|BF262355.2|BF262355 HV_CEa0003M01f Hordeum vulgare seedl... 42 0.038
gb|BF260419.3|BF260419 HVSMEf0021P03f Hordeum vulgare seedl... 42 0.038
gb|CA012886.1|CA012886 HT06L22r HT Hordeum vulgare subsp. v... 42 0.038
gb|BG416061.2|BG416061 HVSMEk0009J20f Hordeum vulgare testa... 40 0.15
gb|BG417076.2|BG417076 HVSMEk0016B22f Hordeum vulgare testa... 40 0.15
gb|BU986911.1|BU986911 HF13E07r HF Hordeum vulgare subsp. v... 40 0.15
gb|BU990192.1|BU990192 HF24F16r HF Hordeum vulgare subsp. v... 40 0.15
gb|CA029858.1|CA029858 HX05G15r HX Hordeum vulgare subsp. v... 40 0.15
>gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ001_C24 5', mRNA sequence
Length = 602
Score = 155 bits (78), Expect = 4e-036
Identities = 195/234 (83%)
Strand = Plus / Minus
Query: 765 atgaggcagttctggaacagcgccgccgagttgccgaagatgaagtccacggtgcccagc 824
||||||||||||||||||| |||||||||||||||||||||||||| | ||||||
Sbjct: 319 atgaggcagttctggaacacggccgccgagttgccgaagatgaagtcgatggtgccggag 260
Query: 825 acctcgcagtcgcggaagaactgccggttggcgtgcacgtacagcgtgtcctggtacccc 884
| |||||| ||||||||||||||| ||||||||||||||||||||||||| || |
Sbjct: 259 atgacgcagttgcggaagaactgccgccgggcgtgcacgtacagcgtgtcctggaacgcg 200
Query: 885 tcgaaccggcagttgtagaacaccgacttgtcgccctgcacgtgcatcgccaccgcctgg 944
|| |||||||||||| |||| ||||| |||||||||||| || |||||||||||||
Sbjct: 199 tcaaaccggcagttgaagaaggccgacaggtcgccctgcaccctcagcgccaccgcctgg 140
Query: 945 tgcccctccggccccgccgtgttcacgaaccccatcgacttgcagatgaacccg 998
|| ||||| ||||||||||| ||||||||| ||||||||||||||||
Sbjct: 139 tgtttctccgcgcccgccgtgttgtggaaccccatgttcttgcagatgaacccg 86
Score = 61.9 bits (31), Expect = 4e-008
Identities = 37/39 (94%)
Strand = Plus / Minus
Query: 482 gtactcggcgtagtagagcgtcttgaggccgaggtcgcc 520
||||||||||||||||||||||||||| |||| ||||||
Sbjct: 602 gtactcggcgtagtagagcgtcttgagcccgaagtcgcc 564
Score = 44.1 bits (22), Expect = 0.010
Identities = 31/34 (91%)
Strand = Plus / Minus
Query: 695 catgttggggtccgtccgcccttgcgccgtcacc 728
||||||||||||||| ||||| ||||| ||||||
Sbjct: 389 catgttggggtccgtgcgcccgtgcgcggtcacc 356
>gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023L12f, mRNA sequence
Length = 536
Score = 129 bits (65), Expect = 2e-028
Identities = 137/161 (85%)
Strand = Plus / Minus
Query: 765 atgaggcagttctggaacagcgccgccgagttgccgaagatgaagtccacggtgcccagc 824
||||||||||||||||||| |||||||||||||||||||||||||| | ||||||
Sbjct: 168 atgaggcagttctggaacacggccgccgagttgccgaagatgaagtcgatggtgccggag 109
Query: 825 acctcgcagtcgcggaagaactgccggttggcgtgcacgtacagcgtgtcctggtacccc 884
| |||||| ||||||||||||||| ||||||||||||||||||||||||| || |
Sbjct: 108 atgacgcagttgcggaagaactgccgccgggcgtgcacgtacagcgtgtcctggaacgcg 49
Query: 885 tcgaaccggcagttgtagaacaccgacttgtcgccctgcac 925
|| |||||||||||| |||| ||||| ||||||||||||
Sbjct: 48 tcaaaccggcagttgaagaaggccgacaggtcgccctgcac 8
Score = 117 bits (59), Expect = 8e-025
Identities = 83/91 (91%)
Strand = Plus / Minus
Query: 430 agcctggccagttgaccctcttgctggtgccggcgcccgggccggtgttggcgtactcgg 489
|||| |||||| ||||||||||||||||||||||||| |||||| |||| |||||||||
Sbjct: 503 agcccggccaggtgaccctcttgctggtgccggcgccggggccgcggttgccgtactcgg 444
Query: 490 cgtagtagagcgtcttgaggccgaggtcgcc 520
||||||||||||||||||| |||| ||||||
Sbjct: 443 cgtagtagagcgtcttgagcccgaagtcgcc 413
Score = 44.1 bits (22), Expect = 0.010
Identities = 31/34 (91%)
Strand = Plus / Minus
Query: 695 catgttggggtccgtccgcccttgcgccgtcacc 728
||||||||||||||| ||||| ||||| ||||||
Sbjct: 238 catgttggggtccgtgcgcccgtgcgcggtcacc 205
>gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ002_L07 5', mRNA sequence
Length = 372
Score = 69.9 bits (35), Expect = 2e-010
Identities = 101/123 (82%)
Strand = Plus / Minus
Query: 767 gaggcagttctggaacagcgccgccgagttgccgaagatgaagtccacggtgcccagcac 826
||||||||||||||||| || || | ||||||||||| |||||| |||||||| ||
Sbjct: 223 gaggcagttctggaacacggcggcggcgttgccgaagacgaagtcgacggtgccggcgac 164
Query: 827 ctcgcagtcgcggaagaactgccggttggcgtgcacgtacagcgtgtcctggtacccctc 886
|||||||||| ||||||| ||| | |||| |||||||||||||||||| ||||||
Sbjct: 163 gcggcagtcgcggtagaactggcggagcgagtgcgcgtacagcgtgtcctggtgcccctc 104
Query: 887 gaa 889
|||
Sbjct: 103 gaa 101
Score = 46.1 bits (23), Expect = 0.002
Identities = 38/43 (88%)
Strand = Plus / Minus
Query: 932 cgccaccgcctggtgcccctccggccccgccgtgttcacgaac 974
|||||||||||||||| ||||||||||||||||| |||||
Sbjct: 58 cgccaccgcctggtgcttggccggccccgccgtgttctcgaac 16
>gb|CK122193.1|CK122193 BES1824101p15 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P151 5-PRIME, mRNA sequence
Length = 641
Score = 61.9 bits (31), Expect = 4e-008
Identities = 34/35 (97%)
Strand = Plus / Minus
Query: 786 gccgccgagttgccgaagatgaagtccacggtgcc 820
||||||||||||||||||||||||||||| |||||
Sbjct: 398 gccgccgagttgccgaagatgaagtccaccgtgcc 364
Score = 58.0 bits (29), Expect = 6e-007
Identities = 35/37 (94%)
Strand = Plus / Minus
Query: 932 cgccaccgcctggtgcccctccggccccgccgtgttc 968
|||||||||||||||| | ||||||||||||||||||
Sbjct: 252 cgccaccgcctggtgcgcgtccggccccgccgtgttc 216
Score = 56.0 bits (28), Expect = 3e-006
Identities = 40/44 (90%)
Strand = Plus / Minus
Query: 841 agaactgccggttggcgtgcacgtacagcgtgtcctggtacccc 884
|||||||||| |||||||| |||||||||||||||||| ||||
Sbjct: 343 agaactgccgcatggcgtgcgcgtacagcgtgtcctggtgcccc 300
Score = 50.1 bits (25), Expect = 2e-004
Identities = 25/25 (100%)
Strand = Plus / Minus
Query: 590 gtactccttccacggccggccgagg 614
|||||||||||||||||||||||||
Sbjct: 619 gtactccttccacggccggccgagg 595
>gb|CK122985.1|CK122985 BES1824101k13 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010K131 5-PRIME, mRNA sequence
Length = 611
Score = 61.9 bits (31), Expect = 4e-008
Identities = 34/35 (97%)
Strand = Plus / Minus
Query: 786 gccgccgagttgccgaagatgaagtccacggtgcc 820
||||||||||||||||||||||||||||| |||||
Sbjct: 398 gccgccgagttgccgaagatgaagtccaccgtgcc 364
Score = 58.0 bits (29), Expect = 6e-007
Identities = 35/37 (94%)
Strand = Plus / Minus
Query: 932 cgccaccgcctggtgcccctccggccccgccgtgttc 968
|||||||||||||||| | ||||||||||||||||||
Sbjct: 252 cgccaccgcctggtgcgcgtccggccccgccgtgttc 216
Score = 56.0 bits (28), Expect = 3e-006
Identities = 40/44 (90%)
Strand = Plus / Minus
Query: 841 agaactgccggttggcgtgcacgtacagcgtgtcctggtacccc 884
|||||||||| |||||||| |||||||||||||||||| ||||
Sbjct: 343 agaactgccgcatggcgtgcgcgtacagcgtgtcctggtgcccc 300
>gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ007_C09 5', mRNA sequence
Length = 417
Score = 58.0 bits (29), Expect = 6e-007
Identities = 65/77 (84%)
Strand = Plus / Minus
Query: 426 tggtagcctggccagttgaccctcttgctggtgccggcgcccgggccggtgttggcgtac 485
||||||||||||||||| |||||| |||| ||| | | || | |||| |||| |||||
Sbjct: 177 tggtagcctggccagttcaccctcctgctcgtgtccgacccagccccggagttgtcgtac 118
Query: 486 tcggcgtagtagagcgt 502
|||||||||||||||||
Sbjct: 117 tcggcgtagtagagcgt 101
>gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 5', mRNA sequence
Length = 528
Score = 52.0 bits (26), Expect = 4e-005
Identities = 53/62 (85%)
Strand = Plus / Minus
Query: 828 tcgcagtcgcggaagaactgccggttggcgtgcacgtacagcgtgtcctggtacccctcg 887
||||| |||||||||||||| ||| || ||||||||| || ||||||||||| ||| ||
Sbjct: 356 tcgcactcgcggaagaactgacggagggagtgcacgtagagggtgtcctggtagcccacg 297
Query: 888 aa 889
||
Sbjct: 296 aa 295
>gb|BQ661505.1|BQ661505 HM03L14u HM Hordeum vulgare subsp. vulgare cDNA clone HM03L14
3-PRIME, mRNA sequence
Length = 298
Score = 50.1 bits (25), Expect = 2e-004
Identities = 40/45 (88%)
Strand = Plus / Plus
Query: 458 gccggcgcccgggccggtgttggcgtactcggcgtagtagagcgt 502
||||||||| ||||||| | || |||||||| |||||||||||||
Sbjct: 219 gccggcgccggggccggcgctgccgtactcgccgtagtagagcgt 263
>gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. vulgare cDNA clone HI09G17
3-PRIME, mRNA sequence
Length = 565
Score = 50.1 bits (25), Expect = 2e-004
Identities = 25/25 (100%)
Strand = Plus / Plus
Query: 590 gtactccttccacggccggccgagg 614
|||||||||||||||||||||||||
Sbjct: 350 gtactccttccacggccggccgagg 374
Score = 50.1 bits (25), Expect = 2e-004
Identities = 40/45 (88%)
Strand = Plus / Plus
Query: 458 gccggcgcccgggccggtgttggcgtactcggcgtagtagagcgt 502
||||||||| ||||||| | || |||||||| |||||||||||||
Sbjct: 218 gccggcgccggggccggcgctgccgtactcgccgtagtagagcgt 262
>gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. vulgare cDNA clone HI12E03
3-PRIME, mRNA sequence
Length = 521
Score = 50.1 bits (25), Expect = 2e-004
Identities = 25/25 (100%)
Strand = Plus / Plus
Query: 590 gtactccttccacggccggccgagg 614
|||||||||||||||||||||||||
Sbjct: 351 gtactccttccacggccggccgagg 375
Score = 50.1 bits (25), Expect = 2e-004
Identities = 40/45 (88%)
Strand = Plus / Plus
Query: 458 gccggcgcccgggccggtgttggcgtactcggcgtagtagagcgt 502
||||||||| ||||||| | || |||||||| |||||||||||||
Sbjct: 219 gccggcgccggggccggcgctgccgtactcgccgtagtagagcgt 263
>gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM022cC.01, mRNA sequence
Length = 1239
Score = 42.1 bits (21), Expect = 0.038
Identities = 27/29 (93%)
Strand = Plus / Minus
Query: 590 gtactccttccacggccggccgaggtagg 618
||||| ||||||||||||||| |||||||
Sbjct: 146 gtactgcttccacggccggccaaggtagg 118
>gb|AL450714.1|AL450714 AL450714 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK03O09u 5', mRNA sequence
Length = 681
Score = 42.1 bits (21), Expect = 0.038
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 366 gacgcgccgtcgatgaaggcg 386
|||||||||||||||||||||
Sbjct: 160 gacgcgccgtcgatgaaggcg 140
>gb|BI951306.1|BI951306 HVSMEl0025H05f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0025H05f, mRNA sequence
Length = 587
Score = 42.1 bits (21), Expect = 0.038
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 366 gacgcgccgtcgatgaaggcg 386
|||||||||||||||||||||
Sbjct: 121 gacgcgccgtcgatgaaggcg 101
>gb|BE216143.1|BE216143 HV_CEb0009J07f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0009J07f, mRNA sequence
Length = 569
Score = 42.1 bits (21), Expect = 0.038
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 366 gacgcgccgtcgatgaaggcg 386
|||||||||||||||||||||
Sbjct: 163 gacgcgccgtcgatgaaggcg 143
>gb|BF262355.2|BF262355 HV_CEa0003M01f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0003M01f, mRNA sequence
Length = 614
Score = 42.1 bits (21), Expect = 0.038
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 366 gacgcgccgtcgatgaaggcg 386
|||||||||||||||||||||
Sbjct: 145 gacgcgccgtcgatgaaggcg 125
>gb|BF260419.3|BF260419 HVSMEf0021P03f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0021P03f, mRNA sequence
Length = 832
Score = 42.1 bits (21), Expect = 0.038
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 366 gacgcgccgtcgatgaaggcg 386
|||||||||||||||||||||
Sbjct: 150 gacgcgccgtcgatgaaggcg 130
>gb|CA012886.1|CA012886 HT06L22r HT Hordeum vulgare subsp. vulgare cDNA clone HT06L22
5-PRIME, mRNA sequence
Length = 658
Score = 42.1 bits (21), Expect = 0.038
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 366 gacgcgccgtcgatgaaggcg 386
|||||||||||||||||||||
Sbjct: 153 gacgcgccgtcgatgaaggcg 133
>gb|BG416061.2|BG416061 HVSMEk0009J20f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0009J20f, mRNA sequence
Length = 465
Score = 40.1 bits (20), Expect = 0.15
Identities = 38/44 (86%)
Strand = Plus / Minus
Query: 459 ccggcgcccgggccggtgttggcgtactcggcgtagtagagcgt 502
|||||||| || |||||||| ||||||| |||||||||||||
Sbjct: 129 ccggcgccaggcccggtgttcccgtactccccgtagtagagcgt 86
>gb|BG417076.2|BG417076 HVSMEk0016B22f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0016B22f, mRNA sequence
Length = 401
Score = 40.1 bits (20), Expect = 0.15
Identities = 38/44 (86%)
Strand = Plus / Minus
Query: 459 ccggcgcccgggccggtgttggcgtactcggcgtagtagagcgt 502
|||||||| || |||||||| ||||||| |||||||||||||
Sbjct: 88 ccggcgccaggcccggtgttcccgtactccccgtagtagagcgt 45
>gb|BU986911.1|BU986911 HF13E07r HF Hordeum vulgare subsp. vulgare cDNA clone HF13E07
5-PRIME, mRNA sequence
Length = 354
Score = 40.1 bits (20), Expect = 0.15
Identities = 38/44 (86%)
Strand = Plus / Minus
Query: 459 ccggcgcccgggccggtgttggcgtactcggcgtagtagagcgt 502
|||||||| || |||||||| ||||||| |||||||||||||
Sbjct: 54 ccggcgccaggcccggtgttcccgtactccccgtagtagagcgt 11
>gb|BU990192.1|BU990192 HF24F16r HF Hordeum vulgare subsp. vulgare cDNA clone HF24F16
5-PRIME, mRNA sequence
Length = 463
Score = 40.1 bits (20), Expect = 0.15
Identities = 38/44 (86%)
Strand = Plus / Minus
Query: 459 ccggcgcccgggccggtgttggcgtactcggcgtagtagagcgt 502
|||||||| || |||||||| ||||||| |||||||||||||
Sbjct: 162 ccggcgccaggcccggtgttcccgtactccccgtagtagagcgt 119
>gb|CA029858.1|CA029858 HX05G15r HX Hordeum vulgare subsp. vulgare cDNA clone HX05G15
5-PRIME, mRNA sequence
Length = 638
Score = 40.1 bits (20), Expect = 0.15
Identities = 26/28 (92%)
Strand = Plus / Minus
Query: 794 gttgccgaagatgaagtccacggtgccc 821
|||||||||||||||||| |||| ||||
Sbjct: 168 gttgccgaagatgaagtcgacggagccc 141
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 134,426
Number of Sequences: 312970
Number of extensions: 134426
Number of successful extensions: 38451
Number of sequences better than 0.5: 22
Number of HSP's better than 0.5 without gapping: 22
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 38394
Number of HSP's gapped (non-prelim): 56
length of query: 1085
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1066
effective length of database: 169,188,109
effective search space: 180354524194
effective search space used: 180354524194
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)