BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3712941.2.1
(1473 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ465022.1|BQ465022 HU02G23r HU Hordeum vulgare subsp. v... 341 4e-092
gb|AL505620.1|AL505620 AL505620 Hordeum vulgare Barke roots... 311 4e-083
gb|BE559029.2|BE559029 HV_CEb0022G04f Hordeum vulgare seedl... 291 3e-077
gb|BQ663389.1|BQ663389 HU02G23u HU Hordeum vulgare subsp. v... 272 3e-071
gb|AL502881.1|AL502881 AL502881 Hordeum vulgare Barke roots... 155 5e-036
gb|BM442847.2|BM442847 EBro02_SQ001_I13_R root, 3 week, hyd... 86 4e-015
gb|BM442973.2|BM442973 EBro02_SQ002_H11_R root, 3 week, hyd... 76 4e-012
gb|BI948612.1|BI948612 HVSMEl0010E03f Hordeum vulgare spike... 58 9e-007
gb|BQ762130.1|BQ762130 EBpi01_SQ005_L22_R pistil, 1 DPA, no... 58 9e-007
gb|BF258077.2|BF258077 HVSMEf0014L07f Hordeum vulgare seedl... 52 5e-005
gb|BF259816.3|BF259816 HVSMEf0020F12f Hordeum vulgare seedl... 52 5e-005
gb|AV926999.1|AV926999 AV926999 K. Sato unpublished cDNA li... 40 0.20
gb|AV930954.1|AV930954 AV930954 K. Sato unpublished cDNA li... 40 0.20
gb|AV931833.1|AV931833 AV931833 K. Sato unpublished cDNA li... 40 0.20
>gb|BQ465022.1|BQ465022 HU02G23r HU Hordeum vulgare subsp. vulgare cDNA clone HU02G23
5-PRIME, mRNA sequence
Length = 700
Score = 341 bits (172), Expect = 4e-092
Identities = 350/408 (85%), Gaps = 1/408 (0%)
Strand = Plus / Plus
Query: 27 cggcgatggcgagcctcaccctgccgccggcgccccccaacccacgccaggacgccatcg 86
|||| ||||||||||||||| |||||||||||||||| ||||| ||||||||||||||||
Sbjct: 294 cggcaatggcgagcctcaccttgccgccggcgcccccgaacccccgccaggacgccatcg 353
Query: 87 acctccacaaggccttcaaagggtttggatgtgacagtacaacagggataaacatactta 146
||||||| |||||||||||||||||||| ||||| |||||||||| ||| ||||||
Sbjct: 354 acctccaaaaggccttcaaagggtttggctgtgatagtacaacagtttcaaatatacttg 413
Query: 147 ctcatcgcgattcggggcagcgtggactcattcaacaggaatacagggtctatgtatcat 206
|||| || || || ||| |||||| ||||||||||||||||| ||||||||
Sbjct: 414 ctcaccgtgactcaatgcaacgtggatacattcaacaggaatacaaaa-ctatgtattcc 472
Query: 207 gaggaactctcccaccgtatttcatctgaactcagtggaaaccacaagaaagctatgtcg 266
|||||||| || | ||||| || ||||||||||||||||||||||||||||| ||||||
Sbjct: 473 gaggaactttcgcgtcgtatatcgtctgaactcagtggaaaccacaagaaagcaatgtcg 532
Query: 267 ctgtggattcttgatcctgctggacgtgatgcaactgttttgagagaagctctaaatggt 326
|| ||||||||||||||||||||||||||||| |||||| ||| ||||||||| | || |
Sbjct: 533 ctctggattcttgatcctgctggacgtgatgcgactgttctgaaagaagctctcagtgct 592
Query: 327 gacactatggatctgagagcagccactgagattatatgttccaggacaccatcacagttg 386
|| | | | ||||||| |||||| ||||| || |||||||| ||||||||||||||| ||
Sbjct: 593 gaaagtcttgatctgaaagcagctactgatataatatgttcgaggacaccatcacagctg 652
Query: 387 caaataatgaaacagacttattatgctagattcggtacttatcttgag 434
||||||||||||||||| |||||||| | || |||||||||||||||
Sbjct: 653 caaataatgaaacagacatattatgcaaagtttggtacttatcttgag 700
>gb|AL505620.1|AL505620 AL505620 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09A09V 5', mRNA sequence
Length = 468
Score = 311 bits (157), Expect = 4e-083
Identities = 379/453 (83%)
Strand = Plus / Plus
Query: 516 gaggttgatcctacaattgtgacacatgatgcaaaggatttgtacaaagctggtgagaaa 575
||||||||||| || || |||||||||||||| ||||| | |||||||||||||||||
Sbjct: 16 gaggttgatcccactatagtgacacatgatgcgaaggacctctacaaagctggtgagaag 75
Query: 576 aggctgggtacggacgagaagatttttatccgtgttttcactgaacgcagttgggcacac 635
| |||||| || || ||||| | || ||||| | |||||||||||||| |||||||||
Sbjct: 76 aagctgggcacagatgagaaaaccttcatccgcatcttcactgaacgcagctgggcacac 135
Query: 636 ttggcatctgtttcttctgcttaccatcatatgtatgaccggaaattagagaaggttatc 695
||||| ||||| ||||||||||||| || |||||||| ||| |||| |||||||| |
Sbjct: 136 atggcagctgttgcttctgcttaccagcacatgtatgatcggtcattacagaaggttgtg 195
Query: 696 aagagcgaaacttctggaaactttgaatttgcacttttgactatcctcagatgcgcggaa 755
||||| ||||| ||||||||||||||| |||| || | |||||||||||||| || ||
Sbjct: 196 aagagtgaaacatctggaaactttgaagttgctctgataactatcctcagatgtgctgag 255
Query: 756 aacccagcaaagtattttgctaagctcttacgaaaggccatgaaaggtttaggcactgat 815
|| ||||| ||||||||||||||| | ||| | ||| ||||||||||| |||| ||||||
Sbjct: 256 aatccagctaagtattttgctaaggtgttaaggaagtccatgaaaggtctaggtactgat 315
Query: 816 gacatgacacttataagggtggtggtgacgaggaccgagattgacatgcaatatatcaag 875
|||| ||||||| ||||||| ||||| |||||||| |||||||| ||||| |||||||||
Sbjct: 316 gacaagacacttgtaagggttgtggtaacgaggactgagattgatatgcagtatatcaag 375
Query: 876 gcagagtacttgaagaagtataagaagccattagctgaggctatcaactccgagacatca 935
||||| |||| ||||| || || ||||| |||||||| |||||| | || || ||||||
Sbjct: 376 gcagaatactacaagaaatacaaaaagccgttagctgatgctatccattcagaaacatca 435
Query: 936 ggaaactatcggacgttccttctttcacttgtt 968
||| |||||||||||||| ||||| ||||||
Sbjct: 436 ggaggttatcggacgttcctgctttctcttgtt 468
>gb|BE559029.2|BE559029 HV_CEb0022G04f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0022G04f, mRNA sequence
Length = 669
Score = 291 bits (147), Expect = 3e-077
Identities = 362/433 (83%), Gaps = 3/433 (0%)
Strand = Plus / Plus
Query: 558 tacaaagctggtgagaaaaggctgggtacggacgagaagatttttatccgtgttttcact 617
||||||||||||||||| | |||||| || || ||||| | || ||||| | ||||||
Sbjct: 6 tacaaagctggtgagaagaagctgggcacagatgagaaaaccttcatccgcatcttcact 65
Query: 618 gaacgcagttgggcacacttggcatctgtttcttctgcttaccatcatatgtatgaccgg 677
|||||||| ||||||||| ||||| ||||| ||||||||||||| || |||||||| |||
Sbjct: 66 gaacgcagctgggcacacatggcagctgttgcttctgcttaccagcacatgtatgatcgg 125
Query: 678 aaattagagaaggttatcaagagcgaaacttctggaaactttgaatttgcacttttgact 737
|||| |||||||| | ||||| ||||| ||||||||||||||| |||| || | |||
Sbjct: 126 tcattacagaaggttgtgaagagtgaaacatctggaaactttgaagttgctctgataact 185
Query: 738 atcctcagatgcgcggaaaacccagcaaagtattttgctaagctcttacgaaaggccatg 797
||||||||||| || || || ||||| ||||||||||||||| | ||| | ||| |||||
Sbjct: 186 atcctcagatgtgctgagaatccagctaagtattttgctaaggtgttaaggaagtccatg 245
Query: 798 aaaggtttaggcactgatgacatgacacttataagggtggtggtgacgaggaccgagatt 857
|||||| |||| |||||||||| ||||||| ||||||| ||||| |||||||| ||||||
Sbjct: 246 aaaggtctaggtactgatgacaagacacttgtaagggttgtggtaacgaggactgagatt 305
Query: 858 gacatgcaatatatcaaggcagagtacttgaagaagtataagaagccattagctgaggct 917
|| ||||| |||||||||||||| |||| ||||| || || ||||| |||||||| |||
Sbjct: 306 gatatgcagtatatcaaggcagaatactacaagaaatacaaaaagccgttagctgatgct 365
Query: 918 atcaactccgagacatcaggaaactatcggacgttccttctttcacttgttggtcatggc 977
||| | || || ||||||||| |||||||||||||| ||||| |||||||| | ||
Sbjct: 366 atccattcagaaacatcaggaggttatcggacgttcctgctttctcttgttgg---tagc 422
Query: 978 cattaggctatgt 990
|||||||||||||
Sbjct: 423 cattaggctatgt 435
>gb|BQ663389.1|BQ663389 HU02G23u HU Hordeum vulgare subsp. vulgare cDNA clone HU02G23
3-PRIME, mRNA sequence
Length = 495
Score = 272 bits (137), Expect = 3e-071
Identities = 319/379 (84%), Gaps = 3/379 (0%)
Strand = Plus / Minus
Query: 612 ttcactgaacgcagttgggcacacttggcatctgtttcttctgcttaccatcatatgtat 671
|||||||||||||| ||||||||| ||||| ||||| ||||||||||||| || ||||||
Sbjct: 479 ttcactgaacgcagctgggcacacatggcagctgttgcttctgcttaccagcacatgtat 420
Query: 672 gaccggaaattagagaaggttatcaagagcgaaacttctggaaactttgaatttgcactt 731
|| ||| |||| |||||||| | ||||| ||||| ||||||||||||||| |||| ||
Sbjct: 419 gatcggtcattacagaaggttgtgaagagtgaaacatctggaaactttgaagttgctctg 360
Query: 732 ttgactatcctcagatgcgcggaaaacccagcaaagtattttgctaagctcttacgaaag 791
| |||||||||||||| || || || ||||| ||||||||||||||| | ||| | |||
Sbjct: 359 ataactatcctcagatgtgctgagaatccagctaagtattttgctaaggtgttaaggaag 300
Query: 792 gccatgaaaggtttaggcactgatgacatgacacttataagggtggtggtgacgaggacc 851
||||||||||| |||| |||||||||| ||||||| ||||||| ||||| ||||||||
Sbjct: 299 tccatgaaaggtctaggtactgatgacaagacacttgtaagggttgtggtaacgaggact 240
Query: 852 gagattgacatgcaatatatcaaggcagagtacttgaagaagtataagaagccattagct 911
|||||||| ||||| |||||||||||||| |||| ||||| || || ||||| ||||||
Sbjct: 239 gagattgatatgcagtatatcaaggcagaatactacaagaaatacaaaaagccgttagct 180
Query: 912 gaggctatcaactccgagacatcaggaaactatcggacgttccttctttcacttgttggt 971
|| |||||| | || | ||||||||| |||||||||||||| ||||| ||||||||
Sbjct: 179 gatgctatccattcaaaaacatcaggaggttatcggacgttcctgctttctcttgttgg- 121
Query: 972 catggccattaggctatgt 990
| |||||||||||||||
Sbjct: 120 --tagccattaggctatgt 104
>gb|AL502881.1|AL502881 AL502881 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09A09u 3', mRNA sequence
Length = 658
Score = 155 bits (78), Expect = 5e-036
Identities = 191/227 (84%), Gaps = 4/227 (1%)
Strand = Plus / Minus
Query: 765 aagtattttgctaagctcttacgaaaggccatgaaaggt-ttaggcactgatgacatgac 823
||||||||||||||| | ||| | ||| ||||||||||| |||| |||||||||| |||
Sbjct: 534 aagtattttgctaaggtgttaaggaagtccatgaaaggtcntaggtactgatgacaagac 475
Query: 824 acttataagggtggtggtgacgaggaccgagattgacatgcaatatatcaaggcagagta 883
|||| ||||||| ||||| |||||||| |||||||| ||||| |||||||||||||| ||
Sbjct: 474 acttgtaagggttgtggtaacgaggactgagattgatatgcagtatatcaaggcagaata 415
Query: 884 cttgaagaagtataagaagccattagctgaggctatcaactccgagacatcaggaaacta 943
|| ||||| || || ||||| |||||||| |||||| | || || ||||||||| ||
Sbjct: 414 ctacaagaaatacaaaaagccgttagctgatgctatccattcagaaacatcaggaggtta 355
Query: 944 tcggacgttccttctttcacttgttggtcatggccattaggctatgt 990
|||||||||||| ||||| |||||||| | |||||||||||||||
Sbjct: 354 tcggacgttcctgctttctcttgttgg---tagccattaggctatgt 311
>gb|BM442847.2|BM442847 EBro02_SQ001_I13_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ001_I13 5', mRNA sequence
Length = 445
Score = 85.7 bits (43), Expect = 4e-015
Identities = 114/137 (83%), Gaps = 3/137 (2%)
Strand = Plus / Plus
Query: 854 gattgacatgcaatatatcaaggcagagtacttgaagaagtataagaagccattagctga 913
|||||| ||||| |||||||||||||| |||| ||||| || || ||||| ||||||||
Sbjct: 1 gattgatatgcagtatatcaaggcagaatactacaagaaatacaaaaagccgttagctga 60
Query: 914 ggctatcaactccgagacatcaggaaactatcggacgttccttctttcacttgttggtca 973
|||||| | || || ||||||||| |||||||||||||| ||||| ||||||||
Sbjct: 61 tgctatccattcagaaacatcaggaggttatcggacgttcctgctttctcttgttgg--- 117
Query: 974 tggccattaggctatgt 990
| |||||||||||||||
Sbjct: 118 tagccattaggctatgt 134
>gb|BM442973.2|BM442973 EBro02_SQ002_H11_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ002_H11 5', mRNA sequence
Length = 424
Score = 75.8 bits (38), Expect = 4e-012
Identities = 103/124 (83%), Gaps = 3/124 (2%)
Strand = Plus / Plus
Query: 867 tatatcaaggcagagtacttgaagaagtataagaagccattagctgaggctatcaactcc 926
|||||||||||||| |||| ||||| || || ||||| |||||||| |||||| | ||
Sbjct: 2 tatatcaaggcagaatactacaagaaatacaaaaagccgttagctgatgctatccattca 61
Query: 927 gagacatcaggaaactatcggacgttccttctttcacttgttggtcatggccattaggct 986
|| ||||||||| |||||||||||||| ||||| |||||||| | |||||||||||
Sbjct: 62 gaaacatcaggaggttatcggacgttcctgctttctcttgttgg---tagccattaggct 118
Query: 987 atgt 990
||||
Sbjct: 119 atgt 122
>gb|BI948612.1|BI948612 HVSMEl0010E03f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0010E03f, mRNA sequence
Length = 812
Score = 58.0 bits (29), Expect = 9e-007
Identities = 47/53 (88%)
Strand = Plus / Plus
Query: 71 cgccaggacgccatcgacctccacaaggccttcaaagggtttggatgtgacag 123
||||| |||||||||| |||||||| |||||||||||| ||||| || |||||
Sbjct: 89 cgccacgacgccatcgccctccacagggccttcaaaggttttggttgcgacag 141
>gb|BQ762130.1|BQ762130 EBpi01_SQ005_L22_R pistil, 1 DPA, no treatment, cv Optic, EBpi01
Hordeum vulgare subsp. vulgare cDNA clone
EBpi01_SQ005_L22 5', mRNA sequence
Length = 420
Score = 58.0 bits (29), Expect = 9e-007
Identities = 76/91 (83%), Gaps = 3/91 (3%)
Strand = Plus / Plus
Query: 900 aagccattagctgaggctatcaactccgagacatcaggaaactatcggacgttccttctt 959
||||| |||||||| |||||| | || || ||||||||| |||||||||||||| |||
Sbjct: 5 aagccgttagctgatgctatccattcagaaacatcaggaggttatcggacgttcctgctt 64
Query: 960 tcacttgttggtcatggccattaggctatgt 990
|| |||||||| | |||||||||||||||
Sbjct: 65 tctcttgttgg---tagccattaggctatgt 92
>gb|BF258077.2|BF258077 HVSMEf0014L07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0014L07f, mRNA sequence
Length = 573
Score = 52.0 bits (26), Expect = 5e-005
Identities = 53/62 (85%)
Strand = Plus / Plus
Query: 823 cacttataagggtggtggtgacgaggaccgagattgacatgcaatatatcaaggcagagt 882
|||| ||| ||||||||||||||||||||||| | |||||| | || ||||| | |||||
Sbjct: 489 cactcatacgggtggtggtgacgaggaccgaggtcgacatgaagtacatcaaagtagagt 548
Query: 883 ac 884
||
Sbjct: 549 ac 550
>gb|BF259816.3|BF259816 HVSMEf0020F12f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020F12f, mRNA sequence
Length = 502
Score = 52.0 bits (26), Expect = 5e-005
Identities = 53/62 (85%)
Strand = Plus / Plus
Query: 823 cacttataagggtggtggtgacgaggaccgagattgacatgcaatatatcaaggcagagt 882
|||| ||| ||||||||||||||||||||||| | |||||| | || ||||| | |||||
Sbjct: 83 cactcatacgggtggtggtgacgaggaccgaggtcgacatgaagtacatcaaagtagagt 142
Query: 883 ac 884
||
Sbjct: 143 ac 144
>gb|AV926999.1|AV926999 AV926999 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd26g03 5', mRNA sequence
Length = 364
Score = 40.1 bits (20), Expect = 0.20
Identities = 40/46 (86%), Gaps = 3/46 (6%)
Strand = Plus / Plus
Query: 945 cggacgttccttctttcacttgttggtcatggccattaggctatgt 990
||||||||||| ||||| |||||||| | |||||||||||||||
Sbjct: 2 cggacgttcctgctttctcttgttgg---tagccattaggctatgt 44
>gb|AV930954.1|AV930954 AV930954 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd25p16 3', mRNA sequence
Length = 308
Score = 40.1 bits (20), Expect = 0.20
Identities = 40/46 (86%), Gaps = 3/46 (6%)
Strand = Plus / Minus
Query: 945 cggacgttccttctttcacttgttggtcatggccattaggctatgt 990
||||||||||| ||||| |||||||| | |||||||||||||||
Sbjct: 307 cggacgttcctgctttctcttgttgg---tagccattaggctatgt 265
>gb|AV931833.1|AV931833 AV931833 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd26g03 3', mRNA sequence
Length = 329
Score = 40.1 bits (20), Expect = 0.20
Identities = 40/46 (86%), Gaps = 3/46 (6%)
Strand = Plus / Minus
Query: 945 cggacgttccttctttcacttgttggtcatggccattaggctatgt 990
||||||||||| ||||| |||||||| | |||||||||||||||
Sbjct: 328 cggacgttcctgctttctcttgttgg---tagccattaggctatgt 286
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 128,000
Number of Sequences: 312970
Number of extensions: 128000
Number of successful extensions: 35745
Number of sequences better than 0.5: 14
Number of HSP's better than 0.5 without gapping: 11
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 35715
Number of HSP's gapped (non-prelim): 20
length of query: 1473
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1454
effective length of database: 169,188,109
effective search space: 245999510486
effective search space used: 245999510486
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)