BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3665460.2.1
(649 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM100490.2|BM100490 EBma01_SQ005_G02_R maternal, 4 DPA, ... 135 2e-030
gb|BF624819.3|BF624819 HVSMEa0017N07f Hordeum vulgare seedl... 90 1e-016
gb|BF255465.2|BF255465 HVSMEf0006O24f Hordeum vulgare seedl... 90 1e-016
gb|BF260744.3|BF260744 HVSMEf0022N07f Hordeum vulgare seedl... 90 1e-016
gb|BQ472572.1|BQ472572 HB09C12T BC Hordeum vulgare subsp. v... 82 3e-014
gb|BU968318.1|BU968318 HB07C12r BC Hordeum vulgare subsp. v... 82 3e-014
gb|CB876810.1|CB876810 HP02H19T HP Hordeum vulgare subsp. v... 82 3e-014
gb|CB879498.1|CB879498 HP02H19w HP Hordeum vulgare subsp. v... 82 3e-014
gb|BU969789.1|BU969789 HB12J02r BC Hordeum vulgare subsp. v... 68 4e-010
gb|CA001848.1|CA001848 HS05M10r HS Hordeum vulgare subsp. v... 62 2e-008
gb|BM373115.2|BM373115 EBma04_SQ003_C15_R maternal, 10 DPA,... 60 9e-008
gb|BQ758997.1|BQ758997 EBma07_SQ003_P08_R maternal, 21 DPA,... 60 9e-008
gb|BU970588.1|BU970588 HB15B14r BC Hordeum vulgare subsp. v... 60 9e-008
gb|BU970614.1|BU970614 HB15C18r BC Hordeum vulgare subsp. v... 60 9e-008
gb|CA007888.1|CA007888 HU09E19r HU Hordeum vulgare subsp. v... 60 9e-008
gb|CA017100.1|CA017100 HV14M18u HV Hordeum vulgare subsp. v... 60 9e-008
gb|CK122771.1|CK122771 BES1824105l12 BES1824 Hordeum vulgar... 54 6e-006
gb|BE454186.3|BE454186 HVSMEh0093C24f Hordeum vulgare 5-45 ... 40 0.088
gb|AW983406.3|AW983406 HVSMEg0010I08f Hordeum vulgare pre-a... 38 0.35
gb|BM817326.1|BM817326 HC105D12_T3.ab1 HC Hordeum vulgare s... 38 0.35
gb|BM370898.2|BM370898 EBro04_SQ002_L18_R root, 3 week, sal... 38 0.35
gb|CA001911.1|CA001911 HS05P10r HS Hordeum vulgare subsp. v... 38 0.35
gb|CA002073.1|CA002073 HS06G24r HS Hordeum vulgare subsp. v... 38 0.35
>gb|BM100490.2|BM100490 EBma01_SQ005_G02_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ005_G02 5', mRNA sequence
Length = 321
Score = 135 bits (68), Expect = 2e-030
Identities = 121/139 (87%)
Strand = Plus / Plus
Query: 1 gctcaacgatctgatcctgctcaacagcggcatctcctcctgcatcccgcccgagatcgg 60
|||||| || |||||||| ||| ||||||| |||||||||||||||||||| ||||||||
Sbjct: 170 gctcaatgagctgatcctcctctacagcggaatctcctcctgcatcccgccggagatcgg 229
Query: 61 caagctggccaagctcnccgtgctggacctcagcttcaccagcatcgccggcaccctgcc 120
|||||||| ||||||| ||||||| |||||||||| || | |||||| ||||| |||||
Sbjct: 230 caagctggacaagctcaccgtgctcgacctcagctccaacggcatcgtcggcaagctgcc 289
Query: 121 ggacacgatcggcaacatg 139
||| | ||||||||||||
Sbjct: 290 cgaccccatcggcaacatg 308
>gb|BF624819.3|BF624819 HVSMEa0017N07f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0017N07f, mRNA sequence
Length = 672
Score = 89.7 bits (45), Expect = 1e-016
Identities = 156/193 (80%)
Strand = Plus / Plus
Query: 143 gcgctggagcagctcgacgtcgcgcacaaccgcctcgccggcgagataccggagagcgtc 202
|||||||||||||||||||||||||||||| ||| |||| | ||||||||| | ||
Sbjct: 219 gcgctggagcagctcgacgtcgcgcacaacgagctcaccggacacataccggaggggatc 278
Query: 203 tgcggcctgccgcacctcaagaacttcacctactcgtacaacttcttctgcggcgagccg 262
|| | ||||||| || |||||||||||||| ||||||| ||||||||||||||||
Sbjct: 279 tgtgcgctgccgcggctggccaacttcacctactcatacaactacttctgcggcgagccg 338
Query: 263 caccggtgcctcgaggtgccgcgcgtcgacgaccgccagaactgcatcgccgggcgcccc 322
| |||||| | | | | |||| ||||||||| ||||||||||||||||| || ||
Sbjct: 339 gagcggtgcacggcgcttcggcgcaacgacgaccggcagaactgcatcgccggccggccg 398
Query: 323 gaccagcgcccgg 335
|||||||| ||||
Sbjct: 399 gaccagcggccgg 411
>gb|BF255465.2|BF255465 HVSMEf0006O24f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0006O24f, mRNA sequence
Length = 603
Score = 89.7 bits (45), Expect = 1e-016
Identities = 156/193 (80%)
Strand = Plus / Plus
Query: 143 gcgctggagcagctcgacgtcgcgcacaaccgcctcgccggcgagataccggagagcgtc 202
|||||||||||||||||||||||||||||| ||| |||| | ||||||||| | ||
Sbjct: 320 gcgctggagcagctcgacgtcgcgcacaacgagctcaccggacacataccggaggggatc 379
Query: 203 tgcggcctgccgcacctcaagaacttcacctactcgtacaacttcttctgcggcgagccg 262
|| | ||||||| || |||||||||||||| ||||||| ||||||||||||||||
Sbjct: 380 tgtgcgctgccgcggctggccaacttcacctactcatacaactacttctgcggcgagccg 439
Query: 263 caccggtgcctcgaggtgccgcgcgtcgacgaccgccagaactgcatcgccgggcgcccc 322
| |||||| | | | | |||| ||||||||| ||||||||||||||||| || ||
Sbjct: 440 gagcggtgcacggcgcttcggcgcaacgacgaccggcagaactgcatcgccggccggccg 499
Query: 323 gaccagcgcccgg 335
|||||||| ||||
Sbjct: 500 gaccagcggccgg 512
>gb|BF260744.3|BF260744 HVSMEf0022N07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0022N07f, mRNA sequence
Length = 674
Score = 89.7 bits (45), Expect = 1e-016
Identities = 156/193 (80%)
Strand = Plus / Plus
Query: 143 gcgctggagcagctcgacgtcgcgcacaaccgcctcgccggcgagataccggagagcgtc 202
|||||||||||||||||||||||||||||| ||| |||| | ||||||||| | ||
Sbjct: 219 gcgctggagcagctcgacgtcgcgcacaacgagctcaccggacacataccggaggggatc 278
Query: 203 tgcggcctgccgcacctcaagaacttcacctactcgtacaacttcttctgcggcgagccg 262
|| | ||||||| || |||||||||||||| ||||||| ||||||||||||||||
Sbjct: 279 tgtgcgctgccgcggctggccaacttcacctactcatacaactacttctgcggcgagccg 338
Query: 263 caccggtgcctcgaggtgccgcgcgtcgacgaccgccagaactgcatcgccgggcgcccc 322
| |||||| | | | | |||| ||||||||| ||||||||||||||||| || ||
Sbjct: 339 gagcggtgcacggcgcttcggcgcaacgacgaccggcagaactgcatcgccggccggccg 398
Query: 323 gaccagcgcccgg 335
|||||||| ||||
Sbjct: 399 gaccagcggccgg 411
>gb|BQ472572.1|BQ472572 HB09C12T BC Hordeum vulgare subsp. vulgare cDNA clone HB09C12
5-PRIME, mRNA sequence
Length = 486
Score = 81.8 bits (41), Expect = 3e-014
Identities = 155/193 (80%)
Strand = Plus / Plus
Query: 143 gcgctggagcagctcgacgtcgcgcacaaccgcctcgccggcgagataccggagagcgtc 202
|||||||||||||||||||||||||||||| ||| |||| | ||||||||| | ||
Sbjct: 13 gcgctggagcagctcgacgtcgcgcacaacgagctcaccggacacataccggaggggatc 72
Query: 203 tgcggcctgccgcacctcaagaacttcacctactcgtacaacttcttctgcggcgagccg 262
|| | ||||||| || |||||||||||||| ||||||| |||||||||||||| |
Sbjct: 73 tgtgcgctgccgcggctggccaacttcacctactcatacaactacttctgcggcgagctg 132
Query: 263 caccggtgcctcgaggtgccgcgcgtcgacgaccgccagaactgcatcgccgggcgcccc 322
| |||||| | | | | |||| ||||||||| ||||||||||||||||| || ||
Sbjct: 133 gagcggtgcacggcgcttcggcgcaacgacgaccggcagaactgcatcgccggccggccg 192
Query: 323 gaccagcgcccgg 335
|||||||| ||||
Sbjct: 193 gaccagcggccgg 205
>gb|BU968318.1|BU968318 HB07C12r BC Hordeum vulgare subsp. vulgare cDNA clone HB07C12
5-PRIME, mRNA sequence
Length = 504
Score = 81.8 bits (41), Expect = 3e-014
Identities = 155/193 (80%)
Strand = Plus / Plus
Query: 143 gcgctggagcagctcgacgtcgcgcacaaccgcctcgccggcgagataccggagagcgtc 202
|||||||||||||||||||||||||||||| ||| |||| | ||||||||| | ||
Sbjct: 13 gcgctggagcagctcgacgtcgcgcacaacgagctcaccggacacataccggaggggatc 72
Query: 203 tgcggcctgccgcacctcaagaacttcacctactcgtacaacttcttctgcggcgagccg 262
|| | ||||||| || |||||||||||||| ||||||| |||||||||||||| |
Sbjct: 73 tgtgcgctgccgcggctggccaacttcacctactcatacaactacttctgcggcgagctg 132
Query: 263 caccggtgcctcgaggtgccgcgcgtcgacgaccgccagaactgcatcgccgggcgcccc 322
| |||||| | | | | |||| ||||||||| ||||||||||||||||| || ||
Sbjct: 133 gagcggtgcacggcgcttcggcgcaacgacgaccggcagaactgcatcgccggccggccg 192
Query: 323 gaccagcgcccgg 335
|||||||| ||||
Sbjct: 193 gaccagcggccgg 205
>gb|CB876810.1|CB876810 HP02H19T HP Hordeum vulgare subsp. vulgare cDNA clone HP02H19
5-PRIME, mRNA sequence
Length = 648
Score = 81.8 bits (41), Expect = 3e-014
Identities = 155/193 (80%)
Strand = Plus / Plus
Query: 143 gcgctggagcagctcgacgtcgcgcacaaccgcctcgccggcgagataccggagagcgtc 202
|||||||||||||||||||||||||||||| ||| |||| | ||||||||| | ||
Sbjct: 344 gcgctggagcagctcgacgtcgcgcacaacgagctcaccggacacataccggaggggatc 403
Query: 203 tgcggcctgccgcacctcaagaacttcacctactcgtacaacttcttctgcggcgagccg 262
|| | ||||||| || |||||||||||||| ||||||| |||||||||||||| |
Sbjct: 404 tgtgcgctgccgcggctggccaacttcacctactcatacaactacttctgcggcgagctg 463
Query: 263 caccggtgcctcgaggtgccgcgcgtcgacgaccgccagaactgcatcgccgggcgcccc 322
| |||||| | | | | |||| ||||||||| ||||||||||||||||| || ||
Sbjct: 464 gagcggtgcacggcgcttcggcgcaacgacgaccggcagaactgcatcgccggccggccg 523
Query: 323 gaccagcgcccgg 335
|||||||| ||||
Sbjct: 524 gaccagcggccgg 536
>gb|CB879498.1|CB879498 HP02H19w HP Hordeum vulgare subsp. vulgare cDNA clone HP02H19
3-PRIME, mRNA sequence
Length = 648
Score = 81.8 bits (41), Expect = 3e-014
Identities = 155/193 (80%)
Strand = Plus / Minus
Query: 143 gcgctggagcagctcgacgtcgcgcacaaccgcctcgccggcgagataccggagagcgtc 202
|||||||||||||||||||||||||||||| ||| |||| | ||||||||| | ||
Sbjct: 356 gcgctggagcagctcgacgtcgcgcacaacgagctcaccggacacataccggaggggatc 297
Query: 203 tgcggcctgccgcacctcaagaacttcacctactcgtacaacttcttctgcggcgagccg 262
|| | ||||||| || |||||||||||||| ||||||| |||||||||||||| |
Sbjct: 296 tgtgcgctgccgcggctggccaacttcacctactcatacaactacttctgcggcgagctg 237
Query: 263 caccggtgcctcgaggtgccgcgcgtcgacgaccgccagaactgcatcgccgggcgcccc 322
| |||||| | | | | |||| ||||||||| ||||||||||||||||| || ||
Sbjct: 236 gagcggtgcacggcgcttcggcgcaacgacgaccggcagaactgcatcgccggccggccg 177
Query: 323 gaccagcgcccgg 335
|||||||| ||||
Sbjct: 176 gaccagcggccgg 164
>gb|BU969789.1|BU969789 HB12J02r BC Hordeum vulgare subsp. vulgare cDNA clone HB12J02
5-PRIME, mRNA sequence
Length = 421
Score = 67.9 bits (34), Expect = 4e-010
Identities = 49/54 (90%)
Strand = Plus / Plus
Query: 14 atcctgctcaacagcggcatctcctcctgcatcccgcccgagatcggcaagctg 67
||||||||||||| | ||||| ||||||||||||||| |||||||||||||||
Sbjct: 41 atcctgctcaacaccagcatcagctcctgcatcccgccggagatcggcaagctg 94
>gb|CA001848.1|CA001848 HS05M10r HS Hordeum vulgare subsp. vulgare cDNA clone HS05M10
5-PRIME, mRNA sequence
Length = 451
Score = 61.9 bits (31), Expect = 2e-008
Identities = 97/119 (81%)
Strand = Plus / Plus
Query: 145 gctggagcagctcgacgtcgcgcacaaccgcctcgccggcgagataccggagagcgtctg 204
||||||||||||| ||||||||||||||| ||| | || |||||| || ||| ||
Sbjct: 294 gctggagcagctcaacgtcgcgcacaacctgctctctgggccgataccccaggccgtgtg 353
Query: 205 cggcctgccgcacctcaagaacttcacctactcgtacaacttcttctgcggcgagccgc 263
|| ||||||| |||||||||||||||| ||| |||||||||||| ||| |||||||
Sbjct: 354 cgcgctgccgcgactcaagaacttcaccttctcatacaacttcttcaccggggagccgc 412
>gb|BM373115.2|BM373115 EBma04_SQ003_C15_R maternal, 10 DPA, no treatment, cv Optic, EBma04
Hordeum vulgare subsp. vulgare cDNA clone
EBma04_SQ003_C15 5', mRNA sequence
Length = 486
Score = 60.0 bits (30), Expect = 9e-008
Identities = 42/46 (91%)
Strand = Plus / Plus
Query: 218 ctcaagaacttcacctactcgtacaacttcttctgcggcgagccgc 263
|||||||||||||||| ||| |||||||||||| |||||||||||
Sbjct: 31 ctcaagaacttcaccttctcctacaacttcttcaccggcgagccgc 76
>gb|BQ758997.1|BQ758997 EBma07_SQ003_P08_R maternal, 21 DPA, no treatment, cv Optic, EBma07
Hordeum vulgare subsp. vulgare cDNA clone
EBma07_SQ003_P08 5', mRNA sequence
Length = 279
Score = 60.0 bits (30), Expect = 9e-008
Identities = 42/46 (91%)
Strand = Plus / Plus
Query: 218 ctcaagaacttcacctactcgtacaacttcttctgcggcgagccgc 263
|||||||||||||||| ||| |||||||||||| |||||||||||
Sbjct: 15 ctcaagaacttcaccttctcctacaacttcttcaccggcgagccgc 60
>gb|BU970588.1|BU970588 HB15B14r BC Hordeum vulgare subsp. vulgare cDNA clone HB15B14
5-PRIME, mRNA sequence
Length = 508
Score = 60.0 bits (30), Expect = 9e-008
Identities = 30/30 (100%)
Strand = Plus / Plus
Query: 143 gcgctggagcagctcgacgtcgcgcacaac 172
||||||||||||||||||||||||||||||
Sbjct: 444 gcgctggagcagctcgacgtcgcgcacaac 473
>gb|BU970614.1|BU970614 HB15C18r BC Hordeum vulgare subsp. vulgare cDNA clone HB15C18
5-PRIME, mRNA sequence
Length = 511
Score = 60.0 bits (30), Expect = 9e-008
Identities = 30/30 (100%)
Strand = Plus / Plus
Query: 143 gcgctggagcagctcgacgtcgcgcacaac 172
||||||||||||||||||||||||||||||
Sbjct: 444 gcgctggagcagctcgacgtcgcgcacaac 473
>gb|CA007888.1|CA007888 HU09E19r HU Hordeum vulgare subsp. vulgare cDNA clone HU09E19
5-PRIME, mRNA sequence
Length = 516
Score = 60.0 bits (30), Expect = 9e-008
Identities = 42/46 (91%)
Strand = Plus / Plus
Query: 218 ctcaagaacttcacctactcgtacaacttcttctgcggcgagccgc 263
|||||||||||||||| ||| |||||||||||| |||||||||||
Sbjct: 118 ctcaagaacttcaccttctcctacaacttcttcaccggcgagccgc 163
>gb|CA017100.1|CA017100 HV14M18u HV Hordeum vulgare subsp. vulgare cDNA clone HV14M18
3-PRIME, mRNA sequence
Length = 576
Score = 60.0 bits (30), Expect = 9e-008
Identities = 42/46 (91%)
Strand = Plus / Plus
Query: 218 ctcaagaacttcacctactcgtacaacttcttctgcggcgagccgc 263
|||||||||||||||| ||| |||||||||||| |||||||||||
Sbjct: 397 ctcaagaacttcaccttctcctacaacttcttcaccggcgagccgc 442
>gb|CK122771.1|CK122771 BES1824105l12 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010L125 5-PRIME, mRNA sequence
Length = 786
Score = 54.0 bits (27), Expect = 6e-006
Identities = 36/39 (92%)
Strand = Plus / Plus
Query: 197 agcgtctgcggcctgccgcacctcaagaacttcacctac 235
|||||||||||||||||| ||||| |||||||||||||
Sbjct: 4 agcgtctgcggcctgccgagcctcaggaacttcacctac 42
>gb|BE454186.3|BE454186 HVSMEh0093C24f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0093C24f, mRNA sequence
Length = 910
Score = 40.1 bits (20), Expect = 0.088
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 142 ggcgctggagcagctcgacg 161
||||||||||||||||||||
Sbjct: 234 ggcgctggagcagctcgacg 253
>gb|AW983406.3|AW983406 HVSMEg0010I08f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0010I08f, mRNA sequence
Length = 821
Score = 38.2 bits (19), Expect = 0.35
Identities = 22/23 (95%)
Strand = Plus / Plus
Query: 408 cttcgccacctccacctccacct 430
|||||||||||||| ||||||||
Sbjct: 242 cttcgccacctccaactccacct 264
>gb|BM817326.1|BM817326 HC105D12_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC105D12_T3.ab1 similar to
Glucokinase,Calcium/calmodulin-dependent protein
kinase,[Myosin light-chain] kinase,Protein C
(activated),Protein kinase,, mRNA sequence
Length = 877
Score = 38.2 bits (19), Expect = 0.35
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 412 gccacctccacctccacct 430
|||||||||||||||||||
Sbjct: 68 gccacctccacctccacct 86
>gb|BM370898.2|BM370898 EBro04_SQ002_L18_R root, 3 week, salt-stressed, cv Optic, EBro04
Hordeum vulgare subsp. vulgare cDNA clone
EBro04_SQ002_L18 5', mRNA sequence
Length = 702
Score = 38.2 bits (19), Expect = 0.35
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 411 cgccacctccacctccacc 429
|||||||||||||||||||
Sbjct: 90 cgccacctccacctccacc 72
>gb|CA001911.1|CA001911 HS05P10r HS Hordeum vulgare subsp. vulgare cDNA clone HS05P10
5-PRIME, mRNA sequence
Length = 569
Score = 38.2 bits (19), Expect = 0.35
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 412 gccacctccacctccacct 430
|||||||||||||||||||
Sbjct: 224 gccacctccacctccacct 242
>gb|CA002073.1|CA002073 HS06G24r HS Hordeum vulgare subsp. vulgare cDNA clone HS06G24
5-PRIME, mRNA sequence
Length = 522
Score = 38.2 bits (19), Expect = 0.35
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 412 gccacctccacctccacct 430
|||||||||||||||||||
Sbjct: 225 gccacctccacctccacct 243
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 94,624
Number of Sequences: 312970
Number of extensions: 94624
Number of successful extensions: 30331
Number of sequences better than 0.5: 23
Number of HSP's better than 0.5 without gapping: 23
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 30270
Number of HSP's gapped (non-prelim): 58
length of query: 649
length of database: 175,134,539
effective HSP length: 19
effective length of query: 630
effective length of database: 169,188,109
effective search space: 106588508670
effective search space used: 106588508670
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)