BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3198841.2.2
(602 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU987196.1|BU987196 HF14B07r HF Hordeum vulgare subsp. v... 321 1e-086
gb|BU971843.1|BU971843 HB19N11r BC Hordeum vulgare subsp. v... 315 9e-085
gb|BG342914.2|BG342914 HVSMEg0001E07f Hordeum vulgare pre-a... 248 2e-064
gb|DW474945.1|DW474945 22-n221 Subtracted libraries from ep... 208 2e-052
gb|AV909143.1|AV909143 AV909143 K. Sato unpublished cDNA li... 196 6e-049
gb|AL505025.1|AL505025 AL505025 Hordeum vulgare Barke roots... 186 6e-046
gb|AV923246.1|AV923246 AV923246 K. Sato unpublished cDNA li... 186 6e-046
gb|CA012048.1|CA012048 HT04E17r HT Hordeum vulgare subsp. v... 161 3e-038
gb|CB882449.1|CB882449 HL01O07w HL Hordeum vulgare subsp. v... 111 3e-023
gb|BI950307.1|BI950307 HVSMEl0020C23f Hordeum vulgare spike... 103 7e-021
gb|AV936335.1|AV936335 AV936335 K. Sato unpublished cDNA li... 86 2e-015
gb|CD663353.1|CD663353 UCRHV18_05ah10_b1 Drought-stressed D... 64 6e-009
>gb|BU987196.1|BU987196 HF14B07r HF Hordeum vulgare subsp. vulgare cDNA clone HF14B07
5-PRIME, mRNA sequence
Length = 613
Score = 321 bits (162), Expect = 1e-086
Identities = 353/416 (84%), Gaps = 2/416 (0%)
Strand = Plus / Plus
Query: 175 tcaatggttcaaaccctgatgactatgagaaatttgagaaagggataatgcactatgggt 234
|||||||||| ||| ||||||| ||||| | ||||||||| || ||||| | ||||| |
Sbjct: 23 tcaatggttctaactctgatgagtatgaaagatttgagaagggattaatggagtatggat 82
Query: 235 gtccacattatagaaggagatgccgcataagagctccttgctgcaatgaaatttttgatt 294
|| ||||||| |||| |||||||||||| |||| |||||||||||||| ||||||||||
Sbjct: 83 gtgcacattaccgaagaagatgccgcatacgagcaccttgctgcaatgacatttttgatt 142
Query: 295 gccgacactgccacaatgaaactaagaattccattaaaattgataaaatgaagaggcatg 354
|||| ||||||||||||||| | |||||||| |||| |||||||| | | | ||||
Sbjct: 143 gccggcactgccacaatgaatcaaagaattcgattagaattgatactcctaggcgacatg 202
Query: 355 aacttccacgccatgaagtgcagcaggttgtatgctcattgtgtggcactgaacaggagg 414
||||||||||||||||| |||||||||| ||||||||||||||||||||||||| ||||
Sbjct: 203 aacttccacgccatgaactgcagcaggtcatatgctcattgtgtggcactgaacaagagg 262
Query: 415 tgcgacaagtatgtatcaattgcggtgtttgcatggggaagtacttctgtggtttgtgca 474
| |||||| |||| |||| || ||||| |||||||||||||| ||||||| ||||||
Sbjct: 263 tacgacaaatatgcatcagctgtggtgtatgcatggggaagtatttctgtgaagtgtgca 322
Query: 475 aactcttcgacgatgatgtctctaaacagcagtatcactgcaacggatgtggaatatgta 534
| ||||| || ||||||||||| |||||||||||||||||| | || ||||||||||| |
Sbjct: 323 agctctttgatgatgatgtctcaaaacagcagtatcactgccatggttgtggaatatgca 382
Query: 535 gaatcggaggcagggagaattccttccactgttcaaaa--gtggatgttgctattc 588
|||| || ||||||||||||| ||| ||||| ||||| |||||||||| |||||
Sbjct: 383 gaattggtggcagggagaatttctttcactgctcaaanatgtggatgttgttattc 438
>gb|BU971843.1|BU971843 HB19N11r BC Hordeum vulgare subsp. vulgare cDNA clone HB19N11
5-PRIME, mRNA sequence
Length = 633
Score = 315 bits (159), Expect = 9e-085
Identities = 333/391 (85%)
Strand = Plus / Plus
Query: 175 tcaatggttcaaaccctgatgactatgagaaatttgagaaagggataatgcactatgggt 234
|||||||||| ||| ||||||| ||||| | ||||||||| || ||||| | ||||| |
Sbjct: 240 tcaatggttctaactctgatgagtatgaaagatttgagaagggattaatggagtatggat 299
Query: 235 gtccacattatagaaggagatgccgcataagagctccttgctgcaatgaaatttttgatt 294
|| ||||||| |||| |||||||||||| |||| |||||||||||||| ||||||||||
Sbjct: 300 gtgcacattaccgaagaagatgccgcatacgagcaccttgctgcaatgacatttttgatt 359
Query: 295 gccgacactgccacaatgaaactaagaattccattaaaattgataaaatgaagaggcatg 354
|||| ||||||||||||||| | |||||||| |||| |||||||| | | | ||||
Sbjct: 360 gccggcactgccacaatgaatcaaagaattcgattagaattgatactcctaggcgacatg 419
Query: 355 aacttccacgccatgaagtgcagcaggttgtatgctcattgtgtggcactgaacaggagg 414
||||||||||||||||| |||||||||| ||||||||||||||||||||||||| ||||
Sbjct: 420 aacttccacgccatgaactgcagcaggtcatatgctcattgtgtggcactgaacaagagg 479
Query: 415 tgcgacaagtatgtatcaattgcggtgtttgcatggggaagtacttctgtggtttgtgca 474
| |||||| |||| |||| || ||||| |||||||||||||| ||||||| ||||||
Sbjct: 480 tacgacaaatatgcatcagctgtggtgtatgcatggggaagtatttctgtgaagtgtgca 539
Query: 475 aactcttcgacgatgatgtctctaaacagcagtatcactgcaacggatgtggaatatgta 534
| ||||| || ||||||||||| |||||||||||||||||| | || ||||||||||| |
Sbjct: 540 agctctttgatgatgatgtctcaaaacagcagtatcactgccatggttgtggaatatgca 599
Query: 535 gaatcggaggcagggagaattccttccactg 565
|||| || ||||||||||||| ||| |||||
Sbjct: 600 gaattggtggcagggagaatttctttcactg 630
>gb|BG342914.2|BG342914 HVSMEg0001E07f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0001E07f, mRNA sequence
Length = 823
Score = 248 bits (125), Expect = 2e-064
Identities = 344/416 (82%), Gaps = 2/416 (0%)
Strand = Plus / Plus
Query: 175 tcaatggttcaaaccctgatgactatgagaaatttgagaaagggataatgcactatgggt 234
|||||||||| ||| ||||||| ||||| | ||||||||| || ||||| | ||||| |
Sbjct: 117 tcaatggttctaactctgatgagtatgaaagatttgagaagggattaatggagtatggat 176
Query: 235 gtccacattatagaaggagatgccgcataagagctccttgctgcaatgaaatttttgatt 294
|| ||||||| |||| | |||||||||| ||| |||||||||||||| ||||||||||
Sbjct: 177 gtgcacattaccgaagaaaatgccgcataccagcaccttgctgcaatgacatttttgatt 236
Query: 295 gccgacactgccacaatgaaactaagaattccattaaaattgataaaatgaagaggcatg 354
|||| ||||||||||||||| | |||||||| |||| |||||||| | | | ||||
Sbjct: 237 gccggcactgccacaatgaatcaaagaattcgattagaattgatactcctaggcgacatg 296
Query: 355 aacttccacgccatgaagtgcagcaggttgtatgctcattgtgtggcactgaacaggagg 414
||||| ||||||||||| |||||||||| ||||||||||||||||||||||||| ||||
Sbjct: 297 aactttcacgccatgaactgcagcaggtcatatgctcattgtgtggcactgaacaagagg 356
Query: 415 tgcgacaagtatgtatcaattgcggtgtttgcatggggaagtacttctgtggtttgtgca 474
| |||||| |||| |||| || ||||| |||||||||||||| ||||||| || |||
Sbjct: 357 tacgacaaatatgcatcagctgtggtgtatgcatggggaagtatttctgtgaaatgagca 416
Query: 475 aactcttcgacgatgatgtctctaaacagcagtatcactgcaacggatgtggaatatgta 534
| ||||| || ||||||| ||| ||||||||| |||||||| | || ||||||||||| |
Sbjct: 417 agctctttgatgatgatggctcaaaacagcaggatcactgccatggttgtggaatatgca 476
Query: 535 gaatcggaggcagggagaattccttccact--gttcaaaagtggatgttgctattc 588
|| || |||||||| |||| ||| |||| ||| ||||||||||| || |||||
Sbjct: 477 aaaatggtggcagggaaaatttctttcacttggttaaaaagtggatggtgttattc 532
>gb|DW474945.1|DW474945 22-n221 Subtracted libraries from epidermis of 7 day old barley
plants inoculated with R. secalis or the avirulence
protein NIP1 Hordeum vulgare subsp. vulgare cDNA clone
22-n221 similar to Best hit to zinc finger protein
ZFP-like in rice (BAD87761), mRNA sequence
Length = 689
Score = 208 bits (105), Expect = 2e-052
Identities = 207/241 (85%)
Strand = Plus / Plus
Query: 175 tcaatggttcaaaccctgatgactatgagaaatttgagaaagggataatgcactatgggt 234
|||||||||| ||| ||||||| ||||| | ||||||||| || ||||| | ||||| |
Sbjct: 449 tcaatggttctaactctgatgagtatgaaagatttgagaagggattaatggagtatggat 508
Query: 235 gtccacattatagaaggagatgccgcataagagctccttgctgcaatgaaatttttgatt 294
|| ||||||| |||| |||||||||||| |||| |||||||||||||| ||||||||||
Sbjct: 509 gtgcacattaccgaagaagatgccgcatacgagcaccttgctgcaatgacatttttgatt 568
Query: 295 gccgacactgccacaatgaaactaagaattccattaaaattgataaaatgaagaggcatg 354
|||| ||||||||||||||| | |||||||| |||| |||||||| | | | ||||
Sbjct: 569 gccggcactgccacaatgaatcaaagaattcgattagaattgatactcctaggcgacatg 628
Query: 355 aacttccacgccatgaagtgcagcaggttgtatgctcattgtgtggcactgaacaggagg 414
||||||||||||||||| |||||||||| ||||||||||||||||||||||||||||||
Sbjct: 629 aacttccacgccatgaactgcagcaggtcatatgctcattgtgtggcactgaacaggagg 688
Query: 415 t 415
|
Sbjct: 689 t 689
>gb|AV909143.1|AV909143 AV909143 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11m03 5', mRNA sequence
Length = 622
Score = 196 bits (99), Expect = 6e-049
Identities = 201/235 (85%)
Strand = Plus / Plus
Query: 175 tcaatggttcaaaccctgatgactatgagaaatttgagaaagggataatgcactatgggt 234
|||||||||| ||| ||||||| ||||| | ||||||||| || ||||| | ||||| |
Sbjct: 387 tcaatggttctaactctgatgagtatgaaagatttgagaagggattaatggagtatggat 446
Query: 235 gtccacattatagaaggagatgccgcataagagctccttgctgcaatgaaatttttgatt 294
|| ||||||| |||| |||||||||||| |||| |||||||||||||| ||||||||||
Sbjct: 447 gtgcacattaccgaagaagatgccgcatacgagcaccttgctgcaatgacatttttgatt 506
Query: 295 gccgacactgccacaatgaaactaagaattccattaaaattgataaaatgaagaggcatg 354
|||| ||||||||||||||| | |||||||| |||| |||||||| | | | ||||
Sbjct: 507 gccggcactgccacaatgaatcaaagaattcgattagaattgatactcctaggcgacatg 566
Query: 355 aacttccacgccatgaagtgcagcaggttgtatgctcattgtgtggcactgaaca 409
||||||||||||||||| |||||||||| |||||||||||||||||||||||||
Sbjct: 567 aacttccacgccatgaactgcagcaggtcatatgctcattgtgtggcactgaaca 621
>gb|AL505025.1|AL505025 AL505025 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07C11V 5', mRNA sequence
Length = 478
Score = 186 bits (94), Expect = 6e-046
Identities = 185/214 (86%), Gaps = 1/214 (0%)
Strand = Plus / Plus
Query: 376 agcaggttgtatgctcattgtgtggcactgaacaggaggtgcgacaagtatgtatcaatt 435
||||||| ||||||||||||||||||||||||| ||||| |||||| |||| |||| |
Sbjct: 7 agcaggtcatatgctcattgtgtggcactgaacaagaggtacgacaaatatgcatcagct 66
Query: 436 gcggtgtttgcatggggaagtacttctgtggtttgtgcaaactcttcgacgatgatgtct 495
| ||||| |||||||||||||| ||||||| ||||||| ||||| || ||||||||||
Sbjct: 67 gtggtgtatgcatggggaagtatttctgtgaagtgtgcaagctctttgatgatgatgtct 126
Query: 496 ctaaacagcagtatcactgcaacggatgtggaatatgtagaatcggaggcagggagaatt 555
| |||||||||||||||||| | || ||||||||||| ||||| || |||||||||||||
Sbjct: 127 caaaacagcagtatcactgccatggttgtggaatatgcagaattggtggcagggagaatt 186
Query: 556 ccttccactgttcaaaa-gtggatgttgctattc 588
||| ||||| |||||| |||||||||| |||||
Sbjct: 187 tctttcactgctcaaaatgtggatgttgttattc 220
>gb|AV923246.1|AV923246 AV923246 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd12g02 5', mRNA sequence
Length = 683
Score = 186 bits (94), Expect = 6e-046
Identities = 200/234 (85%), Gaps = 1/234 (0%)
Strand = Plus / Plus
Query: 175 tcaatggttcaaaccctgatgactatgagaaatttgagaaagggataatgcactatggg- 233
|||||||||| ||| ||||||| ||||| | ||||||||| || ||||| | ||||||
Sbjct: 450 tcaatggttctaactctgatgagtatgaaagatttgagaagggattaatggagtatggga 509
Query: 234 tgtccacattatagaaggagatgccgcataagagctccttgctgcaatgaaatttttgat 293
||| ||||||| |||| |||||||||||| |||| |||||||||||||| |||||||||
Sbjct: 510 tgtgcacattaccgaagaagatgccgcatacgagcaccttgctgcaatgacatttttgat 569
Query: 294 tgccgacactgccacaatgaaactaagaattccattaaaattgataaaatgaagaggcat 353
||||| ||||||||||||||| | |||||||| |||| |||||||| | | | |||
Sbjct: 570 tgccggcactgccacaatgaatcaaagaattcgattagaattgatactcctaggcgacat 629
Query: 354 gaacttccacgccatgaagtgcagcaggttgtatgctcattgtgtggcactgaa 407
|||||||||||||||||| |||||||||| |||||||||||||||||||||||
Sbjct: 630 gaacttccacgccatgaactgcagcaggtcatatgctcattgtgtggcactgaa 683
>gb|CA012048.1|CA012048 HT04E17r HT Hordeum vulgare subsp. vulgare cDNA clone HT04E17
5-PRIME, mRNA sequence
Length = 554
Score = 161 bits (81), Expect = 3e-038
Identities = 183/217 (84%)
Strand = Plus / Plus
Query: 175 tcaatggttcaaaccctgatgactatgagaaatttgagaaagggataatgcactatgggt 234
|||||||||| ||| ||||||| ||||| | ||||||||| || ||||| | ||||| |
Sbjct: 338 tcaatggttctaactctgatgagtatgaaagatttgagaagggattaatggagtatggat 397
Query: 235 gtccacattatagaaggagatgccgcataagagctccttgctgcaatgaaatttttgatt 294
|| ||||||| |||| |||||||||||| |||| |||||||||||||| ||||||||||
Sbjct: 398 gtgcacattaccgaagaagatgccgcatacgagcaccttgctgcaatgacatttttgatt 457
Query: 295 gccgacactgccacaatgaaactaagaattccattaaaattgataaaatgaagaggcatg 354
|||| ||||||||||||||| | |||||||| |||| |||||||| | | | ||||
Sbjct: 458 gccggcactgccacaatgaatcaaagaattcgattagaattgatactcctaggcgacatg 517
Query: 355 aacttccacgccatgaagtgcagcaggttgtatgctc 391
||||||||||||||||| |||||||||| |||||||
Sbjct: 518 aacttccacgccatgaactgcagcaggtcatatgctc 554
>gb|CB882449.1|CB882449 HL01O07w HL Hordeum vulgare subsp. vulgare cDNA clone HL01O07
3-PRIME, mRNA sequence
Length = 593
Score = 111 bits (56), Expect = 3e-023
Identities = 105/120 (87%), Gaps = 1/120 (0%)
Strand = Plus / Plus
Query: 470 gtgcaaactcttcgacgatgatgtctctaaacagcagtatcactgcaacggatgtggaat 529
|||||| ||||| || ||||||||||| |||||||||||||||||| | || ||||||||
Sbjct: 6 gtgcaagctctttgatgatgatgtctcaaaacagcagtatcactgccatggttgtggaat 65
Query: 530 atgtagaatcggaggcagggagaattccttccactgttcaaaa-gtggatgttgctattc 588
||| ||||| || ||||||||||||| ||| ||||| |||||| |||||||||| |||||
Sbjct: 66 atgcagaattggtggcagggagaatttctttcactgctcaaaatgtggatgttgttattc 125
>gb|BI950307.1|BI950307 HVSMEl0020C23f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0020C23f, mRNA sequence
Length = 466
Score = 103 bits (52), Expect = 7e-021
Identities = 117/139 (84%)
Strand = Plus / Plus
Query: 175 tcaatggttcaaaccctgatgactatgagaaatttgagaaagggataatgcactatgggt 234
|||||||||| ||| ||||||| ||||| | ||||||||| || ||||| | || | |
Sbjct: 328 tcaatggttctaactctgatgagtatgaaagatttgagaagggattaatggagtaatgat 387
Query: 235 gtccacattatagaaggagatgccgcataagagctccttgctgcaatgaaatttttgatt 294
|| ||||||| |||| |||||||||||| |||| |||||||||||||| ||| ||||||
Sbjct: 388 gtgcacattaccgaagaagatgccgcatacgagcaccttgctgcaatgacattnttgatt 447
Query: 295 gccgacactgccacaatga 313
|||| ||||||||||||||
Sbjct: 448 gccggcactgccacaatga 466
>gb|AV936335.1|AV936335 AV936335 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal10p08 3', mRNA sequence
Length = 678
Score = 85.7 bits (43), Expect = 2e-015
Identities = 106/123 (86%), Gaps = 3/123 (2%)
Strand = Plus / Minus
Query: 469 tgtgcaaactcttcgacgatgatgt-ctctaaacagcag-tatcactgcaacggatgtgg 526
||||||| ||||| || |||||||| ||| ||||||||| ||||||||| | || |||||
Sbjct: 674 tgtgcaagctctttgatgatgatgtnctcaaaacagcaggtatcactgccatggttgtgg 615
Query: 527 aatatgtagaatcggaggcagggagaattccttccactgttcaaaa-gtggatgttgcta 585
|||||| ||||| || ||||||||||||| ||| ||||| |||||| |||||||||| ||
Sbjct: 614 aatatgcagaattggtggcagggagaatttctttcactgctcaaaatgtggatgttgtta 555
Query: 586 ttc 588
|||
Sbjct: 554 ttc 552
>gb|CD663353.1|CD663353 UCRHV18_05ah10_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_05ah10, mRNA sequence
Length = 706
Score = 63.9 bits (32), Expect = 6e-009
Identities = 60/68 (88%), Gaps = 1/68 (1%)
Strand = Plus / Minus
Query: 522 tgtggaatatgtagaatcggaggcagggagaattccttccactgttcaaaa-gtggatgt 580
||||||||||| ||||| || ||||||||||||| ||| ||||| |||||| ||||||||
Sbjct: 696 tgtggaatatgcagaattggtggcagggagaatttctttcactgctcaaaatgtggatgt 637
Query: 581 tgctattc 588
|| |||||
Sbjct: 636 tgttattc 629
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 50,747
Number of Sequences: 312970
Number of extensions: 50747
Number of successful extensions: 12842
Number of sequences better than 0.5: 12
Number of HSP's better than 0.5 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 12822
Number of HSP's gapped (non-prelim): 19
length of query: 602
length of database: 175,134,539
effective HSP length: 19
effective length of query: 583
effective length of database: 169,188,109
effective search space: 98636667547
effective search space used: 98636667547
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)