BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3185308.2.1
(1019 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF622346.3|BF622346 HVSMEa0002O08f Hordeum vulgare seedl... 50 1e-004
gb|BF626312.1|BF626312 HVSMEa0018F04f Hordeum vulgare seedl... 46 0.002
gb|BQ462905.1|BQ462905 HI02G03r HI Hordeum vulgare subsp. v... 46 0.002
gb|BQ768669.1|BQ768669 EBro08_SQ012_H11_R root, 3 week, dro... 46 0.002
gb|BU997443.1|BU997443 HI08A04r HI Hordeum vulgare subsp. v... 46 0.002
gb|CV058843.1|CV058843 BNEL41f11 Barley EST endosperm libra... 46 0.002
gb|AL503556.1|AL503556 AL503556 Hordeum vulgare Barke roots... 44 0.009
gb|BF621546.3|BF621546 HVSMEa0011F20f Hordeum vulgare seedl... 44 0.009
gb|BF256379.2|BF256379 HVSMEf0009H01f Hordeum vulgare seedl... 44 0.009
gb|BI958334.1|BI958334 HVSMEn0014H08f Hordeum vulgare rachi... 44 0.009
gb|BI960235.1|BI960235 HVSMEn0023N12f Hordeum vulgare rachi... 44 0.009
gb|BF253497.3|BF253497 HVSMEf0001B16f Hordeum vulgare seedl... 44 0.009
gb|BJ454545.1|BJ454545 BJ454545 K. Sato unpublished cDNA li... 44 0.009
gb|BJ462348.1|BJ462348 BJ462348 K. Sato unpublished cDNA li... 44 0.009
gb|BQ465629.1|BQ465629 HU04D11r HU Hordeum vulgare subsp. v... 44 0.009
gb|BQ762357.1|BQ762357 EBro01_SQ005_H18_R root, 3 week, hyd... 44 0.009
gb|CA005047.1|CA005047 HU12E04u HU Hordeum vulgare subsp. v... 44 0.009
gb|CA006626.1|CA006626 HU05D17r HU Hordeum vulgare subsp. v... 44 0.009
gb|CA020757.1|CA020757 HZ37J16r HZ Hordeum vulgare subsp. v... 44 0.009
gb|CA028316.1|CA028316 HZ61J24r HZ Hordeum vulgare subsp. v... 44 0.009
gb|BG368613.1|BG368613 HVSMEi0019P07f Hordeum vulgare 20 DA... 42 0.035
gb|BI957889.1|BI957889 HVSMEn0011P08f Hordeum vulgare rachi... 42 0.035
gb|AV916436.1|AV916436 AV916436 K. Sato unpublished cDNA li... 42 0.035
gb|AV917412.1|AV917412 AV917412 K. Sato unpublished cDNA li... 42 0.035
gb|AV922591.1|AV922591 AV922591 K. Sato unpublished cDNA li... 42 0.035
gb|CA001705.1|CA001705 HS05F12r HS Hordeum vulgare subsp. v... 42 0.035
gb|CA025455.1|CA025455 HZ52D12r HZ Hordeum vulgare subsp. v... 42 0.035
gb|BM099015.2|BM099015 EBpi05_SQ002_L08_R pistil, 8 DPA, no... 40 0.14
gb|BQ754216.1|BQ754216 EBca01_SQ003_D13_R carpel, pre-anthe... 40 0.14
gb|BQ766939.1|BQ766939 EBro08_SQ007_E13_R root, 3 week, dro... 40 0.14
gb|CA014430.1|CA014430 HT11F06r HT Hordeum vulgare subsp. v... 40 0.14
gb|CA021386.1|CA021386 HZ39P04r HZ Hordeum vulgare subsp. v... 40 0.14
gb|CA023510.1|CA023510 HZ46H12r HZ Hordeum vulgare subsp. v... 40 0.14
>gb|BF622346.3|BF622346 HVSMEa0002O08f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0002O08f, mRNA sequence
Length = 844
Score = 50.1 bits (25), Expect = 1e-004
Identities = 28/29 (96%)
Strand = Plus / Plus
Query: 572 ggtgaccaacgtgggcggcgccggggacg 600
|||||||||||||| ||||||||||||||
Sbjct: 620 ggtgaccaacgtggccggcgccggggacg 648
Score = 46.1 bits (23), Expect = 0.002
Identities = 59/71 (83%)
Strand = Plus / Plus
Query: 473 ggccggcatcgtgcccatcacctaccgcaaggtggcgtgcgagaggaaaggcggcatccg 532
|||||||||||| ||| || ||||| | | |||| |||||| || |||||| ||||||||
Sbjct: 521 ggccggcatcgtacccctctcctacagaagggtgccgtgcgtgaagaaagggggcatccg 580
Query: 533 gtacacgatca 543
|| ||| ||||
Sbjct: 581 gttcaccatca 591
>gb|BF626312.1|BF626312 HVSMEa0018F04f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0018F04f, mRNA sequence
Length = 773
Score = 46.1 bits (23), Expect = 0.002
Identities = 29/31 (93%)
Strand = Plus / Plus
Query: 572 ggtgaccaacgtgggcggcgccggggacgtg 602
|||||||||||||| ||||||||| ||||||
Sbjct: 558 ggtgaccaacgtggccggcgccggcgacgtg 588
>gb|BQ462905.1|BQ462905 HI02G03r HI Hordeum vulgare subsp. vulgare cDNA clone HI02G03
5-PRIME, mRNA sequence
Length = 663
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 109 aggaggaggaggacggcggcggc 131
|||||||||||||||||||||||
Sbjct: 87 aggaggaggaggacggcggcggc 65
>gb|BQ768669.1|BQ768669 EBro08_SQ012_H11_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ012_H11 5', mRNA sequence
Length = 590
Score = 46.1 bits (23), Expect = 0.002
Identities = 29/31 (93%)
Strand = Plus / Plus
Query: 572 ggtgaccaacgtgggcggcgccggggacgtg 602
|||||||||||||| ||||||||| ||||||
Sbjct: 559 ggtgaccaacgtggccggcgccggcgacgtg 589
>gb|BU997443.1|BU997443 HI08A04r HI Hordeum vulgare subsp. vulgare cDNA clone HI08A04
5-PRIME, mRNA sequence
Length = 557
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 110 ggaggaggaggacggcggcggca 132
|||||||||||||||||||||||
Sbjct: 327 ggaggaggaggacggcggcggca 305
>gb|CV058843.1|CV058843 BNEL41f11 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL41f11 5' similar to putative
armadillo repeat containing protein, mRNA sequence
Length = 553
Score = 46.1 bits (23), Expect = 0.002
Identities = 23/23 (100%)
Strand = Plus / Minus
Query: 110 ggaggaggaggacggcggcggca 132
|||||||||||||||||||||||
Sbjct: 460 ggaggaggaggacggcggcggca 438
>gb|AL503556.1|AL503556 AL503556 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW02J12T 5', mRNA sequence
Length = 700
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Plus
Query: 573 gtgaccaacgtgggcggcgccggggacgtg 602
||||||||||||| ||||||||| ||||||
Sbjct: 486 gtgaccaacgtggccggcgccggcgacgtg 515
>gb|BF621546.3|BF621546 HVSMEa0011F20f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0011F20f, mRNA sequence
Length = 825
Score = 44.1 bits (22), Expect = 0.009
Identities = 27/29 (93%)
Strand = Plus / Plus
Query: 572 ggtgaccaacgtgggcggcgccggggacg 600
|||||||||||||| ||||||||| ||||
Sbjct: 620 ggtgaccaacgtggncggcgccggcgacg 648
>gb|BF256379.2|BF256379 HVSMEf0009H01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0009H01f, mRNA sequence
Length = 255
Score = 44.1 bits (22), Expect = 0.009
Identities = 22/22 (100%)
Strand = Plus / Minus
Query: 108 gaggaggaggaggacggcggcg 129
||||||||||||||||||||||
Sbjct: 84 gaggaggaggaggacggcggcg 63
>gb|BI958334.1|BI958334 HVSMEn0014H08f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0014H08f, mRNA sequence
Length = 634
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Plus
Query: 573 gtgaccaacgtgggcggcgccggggacgtg 602
||||||||||||| ||||||||| ||||||
Sbjct: 582 gtgaccaacgtggccggcgccggcgacgtg 611
>gb|BI960235.1|BI960235 HVSMEn0023N12f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0023N12f, mRNA sequence
Length = 667
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Plus
Query: 573 gtgaccaacgtgggcggcgccggggacgtg 602
||||||||||||| ||||||||| ||||||
Sbjct: 364 gtgaccaacgtggccggcgccggcgacgtg 393
>gb|BF253497.3|BF253497 HVSMEf0001B16f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0001B16f, mRNA sequence
Length = 574
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Plus
Query: 573 gtgaccaacgtgggcggcgccggggacgtg 602
||||||||||||| ||||||||| ||||||
Sbjct: 346 gtgaccaacgtggccggcgccggcgacgtg 375
>gb|BJ454545.1|BJ454545 BJ454545 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak24n09 5', mRNA sequence
Length = 654
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Plus
Query: 573 gtgaccaacgtgggcggcgccggggacgtg 602
||||||||||||| ||||||||| ||||||
Sbjct: 283 gtgaccaacgtggccggcgccggcgacgtg 312
>gb|BJ462348.1|BJ462348 BJ462348 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak24n09 3', mRNA sequence
Length = 720
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Minus
Query: 573 gtgaccaacgtgggcggcgccggggacgtg 602
||||||||||||| ||||||||| ||||||
Sbjct: 484 gtgaccaacgtggccggcgccggcgacgtg 455
>gb|BQ465629.1|BQ465629 HU04D11r HU Hordeum vulgare subsp. vulgare cDNA clone HU04D11
5-PRIME, mRNA sequence
Length = 625
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Plus
Query: 573 gtgaccaacgtgggcggcgccggggacgtg 602
||||||||||||| ||||||||| ||||||
Sbjct: 221 gtgaccaacgtggccggcgccggcgacgtg 250
>gb|BQ762357.1|BQ762357 EBro01_SQ005_H18_R root, 3 week, hydroponic grown, no treatment, cv
Optic, EBro01 Hordeum vulgare subsp. vulgare cDNA clone
EBro01_SQ005_H18 5', mRNA sequence
Length = 535
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Plus
Query: 573 gtgaccaacgtgggcggcgccggggacgtg 602
||||||||||||| ||||||||| ||||||
Sbjct: 85 gtgaccaacgtggccggcgccggcgacgtg 114
>gb|CA005047.1|CA005047 HU12E04u HU Hordeum vulgare subsp. vulgare cDNA clone HU12E04
3-PRIME, mRNA sequence
Length = 529
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Plus
Query: 573 gtgaccaacgtgggcggcgccggggacgtg 602
||||||||||||| ||||||||| ||||||
Sbjct: 207 gtgaccaacgtggccggcgccggcgacgtg 236
>gb|CA006626.1|CA006626 HU05D17r HU Hordeum vulgare subsp. vulgare cDNA clone HU05D17
5-PRIME, mRNA sequence
Length = 534
Score = 44.1 bits (22), Expect = 0.009
Identities = 28/30 (93%)
Strand = Plus / Plus
Query: 573 gtgaccaacgtgggcggcgccggggacgtg 602
||||||||||||| ||||||||| ||||||
Sbjct: 426 gtgaccaacgtggccggcgccggcgacgtg 455
>gb|CA020757.1|CA020757 HZ37J16r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ37J16
5-PRIME, mRNA sequence
Length = 544
Score = 44.1 bits (22), Expect = 0.009
Identities = 22/22 (100%)
Strand = Plus / Minus
Query: 108 gaggaggaggaggacggcggcg 129
||||||||||||||||||||||
Sbjct: 78 gaggaggaggaggacggcggcg 57
>gb|CA028316.1|CA028316 HZ61J24r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ61J24
5-PRIME, mRNA sequence
Length = 548
Score = 44.1 bits (22), Expect = 0.009
Identities = 22/22 (100%)
Strand = Plus / Minus
Query: 108 gaggaggaggaggacggcggcg 129
||||||||||||||||||||||
Sbjct: 112 gaggaggaggaggacggcggcg 91
>gb|BG368613.1|BG368613 HVSMEi0019P07f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0019P07f, mRNA sequence
Length = 619
Score = 42.1 bits (21), Expect = 0.035
Identities = 27/29 (93%)
Strand = Plus / Plus
Query: 572 ggtgaccaacgtgggcggcgccggggacg 600
|||||||||||||| ||||||||| ||||
Sbjct: 165 ggtgaccaacgtggccggcgccggcgacg 193
>gb|BI957889.1|BI957889 HVSMEn0011P08f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0011P08f, mRNA sequence
Length = 1124
Score = 42.1 bits (21), Expect = 0.035
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 337 cgtgctacgagctcaagggcc 357
|||||||||||||||||||||
Sbjct: 321 cgtgctacgagctcaagggcc 341
>gb|AV916436.1|AV916436 AV916436 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags17d09 5', mRNA sequence
Length = 536
Score = 42.1 bits (21), Expect = 0.035
Identities = 27/29 (93%)
Strand = Plus / Plus
Query: 572 ggtgaccaacgtgggcggcgccggggacg 600
|||||||||||||| ||||||||| ||||
Sbjct: 328 ggtgaccaacgtggccggcgccggcgacg 356
>gb|AV917412.1|AV917412 AV917412 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags18m24 5', mRNA sequence
Length = 626
Score = 42.1 bits (21), Expect = 0.035
Identities = 27/29 (93%)
Strand = Plus / Plus
Query: 572 ggtgaccaacgtgggcggcgccggggacg 600
|||||||||||||| ||||||||| ||||
Sbjct: 139 ggtgaccaacgtggccggcgccggcgacg 167
>gb|AV922591.1|AV922591 AV922591 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags18m24 3', mRNA sequence
Length = 701
Score = 42.1 bits (21), Expect = 0.035
Identities = 27/29 (93%)
Strand = Plus / Minus
Query: 572 ggtgaccaacgtgggcggcgccggggacg 600
|||||||||||||| ||||||||| ||||
Sbjct: 643 ggtgaccaacgtggccggcgccggcgacg 615
>gb|CA001705.1|CA001705 HS05F12r HS Hordeum vulgare subsp. vulgare cDNA clone HS05F12
5-PRIME, mRNA sequence
Length = 520
Score = 42.1 bits (21), Expect = 0.035
Identities = 27/29 (93%)
Strand = Plus / Plus
Query: 572 ggtgaccaacgtgggcggcgccggggacg 600
|||||||||||||| ||||||||| ||||
Sbjct: 402 ggtgaccaacgtggccggcgccggcgacg 430
>gb|CA025455.1|CA025455 HZ52D12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ52D12
5-PRIME, mRNA sequence
Length = 475
Score = 42.1 bits (21), Expect = 0.035
Identities = 27/29 (93%)
Strand = Plus / Plus
Query: 572 ggtgaccaacgtgggcggcgccggggacg 600
|||||||||||||| ||||||||| ||||
Sbjct: 44 ggtgaccaacgtggccggcgccggcgacg 72
>gb|BM099015.2|BM099015 EBpi05_SQ002_L08_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ002_L08 5', mRNA sequence
Length = 523
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 107 cgaggaggaggaggacggcg 126
||||||||||||||||||||
Sbjct: 139 cgaggaggaggaggacggcg 120
>gb|BQ754216.1|BQ754216 EBca01_SQ003_D13_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ003_D13 5', mRNA sequence
Length = 304
Score = 40.1 bits (20), Expect = 0.14
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 108 gaggaggaggaggacggcggcggc 131
||||||||||||| ||||||||||
Sbjct: 8 gaggaggaggaggccggcggcggc 31
>gb|BQ766939.1|BQ766939 EBro08_SQ007_E13_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ007_E13 5', mRNA sequence
Length = 372
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 110 ggaggaggaggacggcggcg 129
||||||||||||||||||||
Sbjct: 155 ggaggaggaggacggcggcg 136
>gb|CA014430.1|CA014430 HT11F06r HT Hordeum vulgare subsp. vulgare cDNA clone HT11F06
5-PRIME, mRNA sequence
Length = 667
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 110 ggaggaggaggacggcggcg 129
||||||||||||||||||||
Sbjct: 61 ggaggaggaggacggcggcg 42
>gb|CA021386.1|CA021386 HZ39P04r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ39P04
5-PRIME, mRNA sequence
Length = 407
Score = 40.1 bits (20), Expect = 0.14
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 107 cgaggaggaggaggacggcg 126
||||||||||||||||||||
Sbjct: 49 cgaggaggaggaggacggcg 30
>gb|CA023510.1|CA023510 HZ46H12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ46H12
5-PRIME, mRNA sequence
Length = 429
Score = 40.1 bits (20), Expect = 0.14
Identities = 26/28 (92%)
Strand = Plus / Plus
Query: 575 gaccaacgtgggcggcgccggggacgtg 602
||||||||||| ||||||||| ||||||
Sbjct: 323 gaccaacgtggccggcgccggcgacgtg 350
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 194,808
Number of Sequences: 312970
Number of extensions: 194808
Number of successful extensions: 68274
Number of sequences better than 0.5: 33
Number of HSP's better than 0.5 without gapping: 33
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 68194
Number of HSP's gapped (non-prelim): 80
length of query: 1019
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1000
effective length of database: 169,188,109
effective search space: 169188109000
effective search space used: 169188109000
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)